A Clinical Study of CAR-T Cells Treatment for Children With CD19+/CD22+ R/R ALL and Lymphoma

NCT ID: NCT04204161

Last Updated: 2021-02-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE1

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-10-08

Study Completion Date

2024-10-08

Brief Summary

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This is a single arm, open-label, uni-center, phase I study . In this study, Children withCD19+/CD22+ R/R B-cell acute lymphoblastic leukemia or lymphoma will be treated with CAR-T19/CAR-T22 Immunotherapy to determine the safety and efficacy of treatment.

Detailed Description

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Conditions

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Relapsed B-cell Acute Lymphoblastic Leukemia, Childhood Refractory B-cell Acute Lymphoblastic Leukemia, Childhood Relapsed/Refractory B-cell Lymphoma, Childhood

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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CAR-T19/CAR-T22

CAR-T19/CAR-T22 (autologous T cells transduced with CD19 / 22 CAR-ζ/4-1BB vector) will be administered to children with R/R B cell Acute Lymphoblastic Leukemia (ALL) or Lymphoma as an IV infusion on days 0, 1 and 2 in the absence of disease progression or unacceptable toxicity.

Group Type EXPERIMENTAL

CAR-T19/CAR-T22

Intervention Type BIOLOGICAL

According to tumor burden and other conditions, patients will be treated with cyclophosphamide or fludarabine,then,CAR-T cells will be infused 48-72 hours later.The recommand dose is 1x10\^5/kg-2.5x10\^8/kg .

Interventions

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CAR-T19/CAR-T22

According to tumor burden and other conditions, patients will be treated with cyclophosphamide or fludarabine,then,CAR-T cells will be infused 48-72 hours later.The recommand dose is 1x10\^5/kg-2.5x10\^8/kg .

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Male and female subjects with CD19+/CD22+ B cell malignancies who have limited prognosis (several months to \< 2 year survival) with currently available therapies will be enrolled.

1. no available curative treatment options (such as autologous or allogeneic SCT)
2. If patients had receive immunotherapy, they should reach requirments:tumor recurrency or the number of B cells recovered.
3. Patients with recurrence after hematopoietic stem cell transplantation need additional satisfaction: 1) no GvHD and not require immunosuppression;2) stem cell transplantation was completed for at least 4 months, and at least 6 months before the CART reinfusion;
4. Patients must be willing to sign an informed consent.
5. Age:≤18 years.
6. survival\>12 weeks
7. Flow cytometry or IHC showed positive expression of CD19/ CD22 in tumor cells within two months.
8. Routine blood test:hemoglobin\>=90 g/L; platelet\>=50×10\^9/L.
9. Liver function: ALT and AST≤2.5 (ULN) times the upper limits of normal (if abnormal liver function is mainly caused by tumor infiltration, it can ≤5 ULN), bilirubin \<2.0 mg/dl.
10. Renal function:BUN: 9-20mg / dl; serum creatinine\<= 1.5 times upper limits of normal; endogenous creatinine clearance rate\>=50 ml/min
11. Negative serum antibody for EBV, CMV, HIV , syphilis, HBVa nd HCV.
12. Cardiac function: stable hemodynamic and left ventricular ejection fraction (LVEF)\>=55%.
13. ECOG score ≤2。
14. Adequate venous access for apheresis, and no other contraindications for leukapheresis

Exclusion Criteria

1. ECOG \>= 3.
2. Patients with history of T cell tumors .
3. organ failure:heart failure Ⅲ and Ⅳ;The liver reached grade C of child-turcotte .Renal failure and uremia;Respiratory failure;People with impaired consciousness.
4. Acute or chronic GVHD after allogeneic hematopoiesis. Hormone or immunosuppressant was used within 30 days.
5. steroid hormoneswere used before and after blood collection and infusion.
6. HIV infection or active hepatitis B or hepatitis C infection.
7. Uncontrolled active infection.
8. Enrolled to other clinical study in the last 4 weeks.
9. Subjects with systemic auto-immune disease or immunodeficiency.
10. Allergic to cytokines.
11. Definite neuropathic or psychotic patients, including authors of dementia or seizures, history of psychotropic substance abuse and unable to quit, or other substantial lesions that may increase central neurotoxicity.
12. Patients with malignant tumors of the central nervous system.
13. Lung, brain or intestinal tumor infiltrates.
14. The second tumor was found.
15. Allergic to cytokine antagonists.
16. Other patients that researchers considered unsuitable for inclusion.
Minimum Eligible Age

1 Month

Maximum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Xiangya Hospital of Central South University

OTHER

Sponsor Role collaborator

Shenzhen BinDeBio Ltd.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Yang Zhonghua

Role: STUDY_DIRECTOR

Shenzhen BinDeBio Tech Co.,Ltd

Locations

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Xiangya Hospital Central South University

Changsha, Hunan, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Yang Zhonghua

Role: CONTACT

18938688105

Facility Contacts

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Yang Minghua, PhD

Role: primary

13973135843

Other Identifiers

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2019XY-BDB011

Identifier Type: -

Identifier Source: org_study_id

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