Trial Outcomes & Findings for Effects of Platelet Rich Plasma Injections on Biomarkers After Anterior Cruciate Ligament Tears (NCT NCT04088227)

NCT ID: NCT04088227

Last Updated: 2025-02-17

Results Overview

The presence of the Synovial Interleukin (IL) -1 Receptor Antagonist biomarker in the synovial fluid will be assessed.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

24 participants

Primary outcome timeframe

Timepoint 1: initial visit (within 10 days of injury) Timepoint 2: at the time of surgery (within 4 weeks of injury)

Results posted on

2025-02-17

Participant Flow

Participant milestones

Participant milestones
Measure
Control Group
The control group will have a joint aspiration performed at an initial visit (within the first 10 days after injury), and at the time of surgery (within 4 weeks of injury). Control Group: Patients will have their knee aspirated at their initial physician visit and at the time of surgery.
Experimental Group
The experimental group will have a joint aspiration performed at an initial visit (within the first 10 days after injury) and receive an injection of leukocyte poor platelet rich plasma injection in their knee. At a second visit 5-12 days after the initial visit, the patient will receive a joint aspiration and platelet rich plasma injection. A final joint aspiration will be performed at the time of surgery (within 4 weeks of injury). Experimental Group: Patients will receive platelet rich plasma injection two times prior to surgery.
Overall Study
STARTED
12
12
Overall Study
COMPLETED
10
12
Overall Study
NOT COMPLETED
2
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Control Group
The control group will have a joint aspiration performed at an initial visit (within the first 10 days after injury), and at the time of surgery (within 4 weeks of injury). Control Group: Patients will have their knee aspirated at their initial physician visit and at the time of surgery.
Experimental Group
The experimental group will have a joint aspiration performed at an initial visit (within the first 10 days after injury) and receive an injection of leukocyte poor platelet rich plasma injection in their knee. At a second visit 5-12 days after the initial visit, the patient will receive a joint aspiration and platelet rich plasma injection. A final joint aspiration will be performed at the time of surgery (within 4 weeks of injury). Experimental Group: Patients will receive platelet rich plasma injection two times prior to surgery.
Overall Study
Withdrawal by Subject
1
0
Overall Study
Surgery postponed >5weeks
1
0

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Control Group
n=12 Participants
The control group will have a joint aspiration performed at an initial visit (within the first 10 days after injury), and at the time of surgery (within 4 weeks of injury). Control Group: Patients will have their knee aspirated at their initial physician visit and at the time of surgery.
Experimental Group
n=12 Participants
The experimental group will have a joint aspiration performed at an initial visit (within the first 10 days after injury) and receive an injection of leukocyte poor platelet rich plasma injection in their knee. At a second visit 5-12 days after the initial visit, the patient will receive a joint aspiration and platelet rich plasma injection. A final joint aspiration will be performed at the time of surgery (within 4 weeks of injury). Experimental Group: Patients will receive platelet rich plasma injection two times prior to surgery.
Total
n=24 Participants
Total of all reporting groups
Age, Continuous
19.4 years
STANDARD_DEVIATION 8 • n=12 Participants
20 years
STANDARD_DEVIATION 6.7 • n=12 Participants
20 years
STANDARD_DEVIATION 7.7 • n=24 Participants
Sex: Female, Male
Female
3 Participants
n=12 Participants
5 Participants
n=12 Participants
8 Participants
n=24 Participants
Sex: Female, Male
Male
9 Participants
n=12 Participants
7 Participants
n=12 Participants
16 Participants
n=24 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Weight
87.6 kg
STANDARD_DEVIATION 26.7 • n=12 Participants
68.5 kg
STANDARD_DEVIATION 12.8 • n=12 Participants
80.8 kg
STANDARD_DEVIATION 21.56 • n=24 Participants
Height
173 cm
STANDARD_DEVIATION 11.5 • n=12 Participants
174.6 cm
STANDARD_DEVIATION 8.3 • n=12 Participants
174.6 cm
STANDARD_DEVIATION 11.63 • n=24 Participants
BMI
29.1 kg/m^2
STANDARD_DEVIATION 6.8 • n=12 Participants
22.5 kg/m^2
STANDARD_DEVIATION 4 • n=12 Participants
26.2 kg/m^2
STANDARD_DEVIATION 6.48 • n=24 Participants
Time to Surgery (days)
16.9 days
STANDARD_DEVIATION 6.8 • n=12 Participants
27 days
STANDARD_DEVIATION 13.1 • n=12 Participants
20.74 days
STANDARD_DEVIATION 9.34 • n=24 Participants

PRIMARY outcome

Timeframe: Timepoint 1: initial visit (within 10 days of injury) Timepoint 2: at the time of surgery (within 4 weeks of injury)

The presence of the Synovial Interleukin (IL) -1 Receptor Antagonist biomarker in the synovial fluid will be assessed.

Outcome measures

Outcome measures
Measure
Control Group
n=12 Participants
The control group will have a joint aspiration performed at an initial visit (within the first 10 days after injury), and at the time of surgery (within 4 weeks of injury). Control Group: Patients will have their knee aspirated at their initial physician visit and at the time of surgery.
Experimental Group
n=12 Participants
The experimental group will have a joint aspiration performed at an initial visit (within the first 10 days after injury) and receive an injection of leukocyte poor platelet rich plasma injection in their knee. At a second visit 5-12 days after the initial visit, the patient will receive a joint aspiration and platelet rich plasma injection. A final joint aspiration will be performed at the time of surgery (within 4 weeks of injury). Experimental Group: Patients will receive platelet rich plasma injection two times prior to surgery.
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-1ß Timepoint 1
13.8 pg/ml
Standard Deviation 4.1
16 pg/ml
Standard Deviation 4.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-1ß Timepoint 2
9.9 pg/ml
Standard Deviation 4.9
7.5 pg/ml
Standard Deviation 4.5
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-10 Timepoint 1
53.1 pg/ml
Standard Deviation 16.4
64.3 pg/ml
Standard Deviation 21.4
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-10 Timepoint 2
36.1 pg/ml
Standard Deviation 20.3
31.7 pg/ml
Standard Deviation 19.2
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-6 Timepoint 1
7668.6 pg/ml
Standard Deviation 4092.9
8650.8 pg/ml
Standard Deviation 3983.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-6 Timepoint 2
3940.2 pg/ml
Standard Deviation 3809.2
2196.2 pg/ml
Standard Deviation 2994.2
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
GM- CSF Timepoint 1
20.6 pg/ml
Standard Deviation 7.1
25.3 pg/ml
Standard Deviation 9.1
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
GM- CSF Timepoint 2
16 pg/ml
Standard Deviation 10.1
12.4 pg/ml
Standard Deviation 9.5
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-5 Timepoint 1
30.6 pg/ml
Standard Deviation 16.7
48.8 pg/ml
Standard Deviation 23.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-5 Timepoint 2
27.9 pg/ml
Standard Deviation 23.3
24.7 pg/ml
Standard Deviation 19.7
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IFN-γ Timepoint 1
2.5 pg/ml
Standard Deviation 1.1
4.0 pg/ml
Standard Deviation 1.7
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IFN-γ Timepoint 2
2.6 pg/ml
Standard Deviation 1.5
1.7 pg/ml
Standard Deviation 1.3
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
TNF-⍺ Timepoint 1
11.2 pg/ml
Standard Deviation 7.0
21.6 pg/ml
Standard Deviation 9.5
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
TNF-⍺ Timepoint 2
10.4 pg/ml
Standard Deviation 9.1
10.9 pg/ml
Standard Deviation 7.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-2 Timepoint 1
29.2 pg/ml
Standard Deviation 24.9
54.6 pg/ml
Standard Deviation 34.3
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-2 Timepoint 2
32.7 pg/ml
Standard Deviation 32.5
28.6 pg/ml
Standard Deviation 25.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-4 Timepoint 1
278.2 pg/ml
Standard Deviation 144.4
398.5 pg/ml
Standard Deviation 189.4
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-4 Timepoint 2
234.5 pg/ml
Standard Deviation 161.6
200.2 pg/ml
Standard Deviation 167.7
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-8 Timepoint 1
315.1 pg/ml
Standard Deviation 80.3
1295.2 pg/ml
Standard Deviation 1075.9
Change in Synovial Interleukin (IL) -1 Receptor Antagonist Concentration
IL-8 Timepoint 2
124.8 pg/ml
Standard Deviation 49.7
222.6 pg/ml
Standard Deviation 113.4

Adverse Events

Control Group

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Experimental Group

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Director of Research

Andrews Research & Education Foundation

Phone: 8509168570

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place