Recognition of Circulating Tumor DNA in Soft Tissue Sarcoma

NCT ID: NCT03896620

Last Updated: 2025-12-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

50 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-06-18

Study Completion Date

2026-06-01

Brief Summary

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A tissue biopsy is currently the only clinical test or procedure that is able to confirm the initial diagnosis of soft tissue or metastatic sarcoma and evaluate its progress during treatment. However, tissue biopsy collection can be challenging (depending on the location of the tumor), and this procedure poses physical risks to the patient. A tissue biopsy also needs to be recollected at various time points in order to assess if the patient is responding to treatment.

In this project, the investigator would like to collect blood samples in addition to the sample of the tumor that will be collected before treatment is started. The investigator would like to analyze both blood and tumor, in hopes of identifying new biomarkers of sarcoma that can help the study doctors better diagnose sarcoma for patients in the future without needing to collect a piece of their tumor.

A biomarker is something found in the blood, other body fluids, or tissues that can be used to measure the progress of disease, how a treatment is working, or its likelihood of being successful. In this project, the investigator would like to compare ctDNA from blood to the DNA in the tumor. ctDNA, or circulating tumor DNA, is DNA originating from the tumor that is present in the blood. It can be assessed by taking an additional sample of blood when it will be collected for normal laboratory tests. These biomarkers may also help the study doctors detect how a patient is responding to their treatment or help predict their response to future treatment.

Detailed Description

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In the proposed study, the investigators wish to prospectively collect blood samples from 25 patients with localized soft tissue sarcomas and 25 patients with metastatic sarcoma (schema III) at various time points outlined. The presence/absence, as well as the change of ctDNA in circulation, will then be measured after the therapies administered and correlated with overall survival, progression-free survival and local control.

The risks to the subjects enrolled on this study include the general risks of undergoing the standard procedures, such as blood draws, imaging, radiation therapy, chemotherapy and surgery, which will be discussed prior to the therapies and images each patient undergoes. The risks of the procedures are not risks of the study with exception to blood draws. There is also a risk of loss of confidentiality, but the data will be deidentified once acquired. Thus, this risk is minimal.

Conditions

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Sarcoma

Keywords

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Sarcoma ctDNA

Study Design

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Observational Model Type

OTHER

Study Time Perspective

OTHER

Study Groups

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Stage II-III Sarcomas undergoing preoperative radiation therapy (RT)

This group will have preoperative chemotherapy (if administered), preoperative radiation and surgery.

Preoperative RT

Intervention Type DEVICE

Radiation delivered before surgery.

Chemotherapy

Intervention Type DRUG

Physician's choice of chemotherapy.

Surgery

Intervention Type OTHER

Surgical excision of tumor.

Stage II-III Sarcomas undergoing postoperative RT

This group will have surgery, postoperative radiation, post operative chemotherapy (if administered).

Postoperative RT

Intervention Type DEVICE

Radiation delivered after surgery.

Chemotherapy

Intervention Type DRUG

Physician's choice of chemotherapy.

Surgery

Intervention Type OTHER

Surgical excision of tumor.

Stage IV Sarcomas

This group will only have chemotherapy.

Chemotherapy

Intervention Type DRUG

Physician's choice of chemotherapy.

Interventions

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Preoperative RT

Radiation delivered before surgery.

Intervention Type DEVICE

Postoperative RT

Radiation delivered after surgery.

Intervention Type DEVICE

Chemotherapy

Physician's choice of chemotherapy.

Intervention Type DRUG

Surgery

Surgical excision of tumor.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Have signed an Institutional Review Board (IRB)/Independent Ethics Committee (IEC)-approved informed consent form (ICF) prior to any study-specific evaluation.
* Diagnosis of soft tissue sarcoma.
* ≥ 18 years of age.
* Planned biopsy obtained within four weeks after registration.
* History and physical within eight weeks prior to registration.
* For stage II-III patients, no prior therapy to primary site of sarcoma.
* Computerized tomography (CT), positron emission tomography (PET) /CT or MR imaging of the affected primary site obtained in stage II-III or metastatic site in stage IV patients within eight weeks prior to registration.
* CT chest or PET /CT acquired to assess distant disease within eight weeks prior to registration for stage II-III patients.
* Karnofsky Performance Scale (KPS) 60 or above within four weeks prior to registration.
* Documentation of stage within eight weeks prior to registration.
* Biopsy of primary or metastatic disease site must be safe, feasible and in concordance with standard of care per the treating physician and/or radiologist.
* Life expectancy ≥ 12 weeks.

Exclusion Criteria

* Pregnant women.
* Patients with a history of metastatic disease from a primary other than sarcoma.
* Patients who cannot undergo imaging as part of treatment planning or surveillance.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Medical College of Wisconsin

OTHER

Sponsor Role lead

Responsible Party

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Ciani M Ellison, MD

Assistant Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Meena Bedi

Role: PRINCIPAL_INVESTIGATOR

Medical College of Wisconsin

Locations

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Froedtert Hospital & the Medical College of Wisconsin

Milwaukee, Wisconsin, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Medical College of Wisconsin Cancer Center Clinical Trials Office

Role: CONTACT

Phone: 414-805-8900

Email: [email protected]

Facility Contacts

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Meena Bedi, MD

Role: primary

Other Identifiers

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PRO00034441

Identifier Type: -

Identifier Source: org_study_id