Trial Outcomes & Findings for Impact of Steady State Cobicistat and Darunavir/Cobicistat on the Pharmacokinetics and Pharmacodynamics of Oral Anticoagulants (Rivaroxaban, Apixaban) in Health Volunteers (NCT NCT03864406)

NCT ID: NCT03864406

Last Updated: 2023-12-01

Results Overview

Area under the concentration versus time curve from 0 to infinity (AUC0-∞) following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 to measure the total drug exposure was analyzed by the linear up/log down trapezoidal rule using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA).

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

12 participants

Primary outcome timeframe

Total drug exposure at time point zero to infinity on days 1, 7, & 13

Results posted on

2023-12-01

Participant Flow

Participant milestones

Participant milestones
Measure
Pharmacokinetic Study in Healthy Volunteers
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Overall Study
STARTED
12
Overall Study
COMPLETED
12
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Impact of Steady State Cobicistat and Darunavir/Cobicistat on the Pharmacokinetics and Pharmacodynamics of Oral Anticoagulants (Rivaroxaban, Apixaban) in Health Volunteers

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Age, Categorical
<=18 years
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
12 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
Sex: Female, Male
Female
1 Participants
n=5 Participants
Sex: Female, Male
Male
11 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
4 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
8 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
2 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
2 Participants
n=5 Participants
Race (NIH/OMB)
White
6 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=5 Participants

PRIMARY outcome

Timeframe: Total drug exposure at time point zero to infinity on days 1, 7, & 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Area under the concentration versus time curve from 0 to infinity (AUC0-∞) following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 to measure the total drug exposure was analyzed by the linear up/log down trapezoidal rule using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA).

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Area Under the Concentration Versus Time Curve (AUC0-∞) for Rivaroxaban
Day 13 (phase 3)
2226.38 hr*ng/ml
Standard Deviation 918.07
Area Under the Concentration Versus Time Curve (AUC0-∞) for Rivaroxaban
Day 1 (phase 1)
1049.19 hr*ng/ml
Standard Deviation 401.58
Area Under the Concentration Versus Time Curve (AUC0-∞) for Rivaroxaban
Day 7 (phase 2)
2274.3 hr*ng/ml
Standard Deviation 766.51

PRIMARY outcome

Timeframe: 0 to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Area under the concentration versus time curve from (AUC0-24hr) following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 to measure total drug exposure was analyzed by the linear up/log down trapezoidal rule using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA).

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Area Under the Concentration Versus Time Curve (AUC0-24hr) for Rivaroxaban
Day 1 (phase 1)
991.25 hr*ng/ml
Standard Deviation 378.02
Area Under the Concentration Versus Time Curve (AUC0-24hr) for Rivaroxaban
Day 7 (phase 2)
1929.78 hr*ng/ml
Standard Deviation 631.58
Area Under the Concentration Versus Time Curve (AUC0-24hr) for Rivaroxaban
Day 13 (phase 3)
1939 hr*ng/ml
Standard Deviation 748.78

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Maximum total plasma concentration (Cmax) following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA). Cmax for rivaroxaban was obtained directly by visual inspection of the plasma concentration versus time over 24 hours postdose.

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Maximum Total Plasma Concentration (Cmax) for Rivaroxaban
Day 1 (phase 1)
128.6 ng/mL
Standard Deviation 42.39
Maximum Total Plasma Concentration (Cmax) for Rivaroxaban
Day 7 (phase 2)
192.25 ng/mL
Standard Deviation 61.72
Maximum Total Plasma Concentration (Cmax) for Rivaroxaban
Day 13 (phase 3)
196.17 ng/mL
Standard Deviation 62.27

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Time to maximum plasma concentration for Rivaroxaban following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA). Tmax for rivaroxaban was obtained directly by visual inspection of the plasma concentration versus time over 24 hours postdose.

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Time to Maximum Plasma Concentration (Tmax) for Rivaroxaban
Day 1 (phase 1)
2.5 Hours
Standard Deviation 1.38
Time to Maximum Plasma Concentration (Tmax) for Rivaroxaban
Day 7 (phase 2)
4.1 Hours
Standard Deviation 1.26
Time to Maximum Plasma Concentration (Tmax) for Rivaroxaban
Day 13 (phase 3)
3.02 Hours
Standard Deviation 1.29

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Terminal elimination half-life (t½) for Rivaroxaban following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA). The apparent elimination rate constant (λZ) was determined by calculating the absolute value of the slope of the log-linear regression of at least 3 points of the plasma concentration-time plot. The t½ was calculated as 0.693/apparent elimination rate constant (λZ).

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Terminal Elimination Half-life (t½) for Rivaroxaban
Day 1 (phase 1)
5.65 Hours
Standard Deviation 0.84
Terminal Elimination Half-life (t½) for Rivaroxaban
Day 7 (phase 2)
8.19 Hours
Standard Deviation 2.52
Terminal Elimination Half-life (t½) for Rivaroxaban
Day 13 (phase 3)
7.49 Hours
Standard Deviation 1.68

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Apparent oral clearance (CL/F) for Rivaroxaban following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA). CL/F was calculated as 10 mg ÷ AUC0-tau for rivaroxaban.

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Apparent Oral Clearance (CL/F) for Rivaroxaban
Day 1 (phase 1)
11.04 L/hr
Standard Deviation 4.69
Apparent Oral Clearance (CL/F) for Rivaroxaban
Day 7 (phase 2)
4.92 L/hr
Standard Deviation 1.77
Apparent Oral Clearance (CL/F) for Rivaroxaban
Day 13 (phase 3)
5.19 L/hr
Standard Deviation 2.11

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Apparent volume of distribution (V/F) for Rivaroxaban following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA).

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Apparent Volume of Distribution (V/F) for Rivaroxaban
Day 7 (phase 2)
56.22 Liter
Standard Deviation 23.07
Apparent Volume of Distribution (V/F) for Rivaroxaban
Day 13 (phase 3)
52.84 Liter
Standard Deviation 16.32
Apparent Volume of Distribution (V/F) for Rivaroxaban
Day 1 (phase 1)
89.94 Liter
Standard Deviation 42.32

PRIMARY outcome

Timeframe: Up to 24 hours postdose on days 1, 7, and 13

Population: All participants who completed at least one intensive pharmacokinetic visit were included in the pharmacokinetics analysis

Minimum total plasma concentration (Cmin) for Rivaroxaban following a single dose of rivaroxaban 10 mg orally on days 1, 7, and 13 was analyzed using noncompartmental methods with Phoenix WinNonlin® (version 8.0; Pharsight Corporation, Mountain View, CA). Cmin for rivaroxaban was obtained directly by visual inspection of the plasma concentration versus times over 24 hours postdose.

Outcome measures

Outcome measures
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 Participants
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Minimum Total Plasma Concentration (Cmin) for Rivaroxaban
Day 1 (phase 1)
6.93 ng/mL
Standard Deviation 3.43
Minimum Total Plasma Concentration (Cmin) for Rivaroxaban
Day 7 (phase 2)
27.32 ng/mL
Standard Deviation 13.25
Minimum Total Plasma Concentration (Cmin) for Rivaroxaban
Day 13 (phase 3)
24.43 ng/mL
Standard Deviation 14.38

Adverse Events

Pharmacokinetic Study in Healthy Volunteers

Serious events: 0 serious events
Other events: 8 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Pharmacokinetic Study in Healthy Volunteers
n=12 participants at risk
Healthy participants received a single dose of rivaroxaban 10 mg orally on day 1 (phase 1) followed by cobicistat 150 mg once daily from days 2 - 7 and a single dose of rivaroxaban 10 mg on day 7 (phase 2). Participants received darunavir /cobicistat 800/150 mg once daily from days 8 - 13 followed by a single dose of rivaroxaban 10mg on day 13 (phase 3). Serial blood sampling was done on days 1, 7, and 13.
Cardiac disorders
Dizziness
16.7%
2/12 • 23 days
Gastrointestinal disorders
Nausea
8.3%
1/12 • 23 days
General disorders
Fatigue
8.3%
1/12 • 23 days
Investigations
Activated partial thromboplastin time prolonged
8.3%
1/12 • 23 days
Investigations
Blood bicarbonate decreased
41.7%
5/12 • 23 days
Investigations
Blood bilirubin increased
8.3%
1/12 • 23 days
Investigations
Blood phosphorus decreased
8.3%
1/12 • 23 days
Investigations
Blood potassium decreased
8.3%
1/12 • 23 days
Investigations
Glomerular filtration rate decreased
16.7%
2/12 • 23 days
Investigations
Haemoglobin decreased
8.3%
1/12 • 23 days
Musculoskeletal and connective tissue disorders
Neck pain
8.3%
1/12 • 23 days
Nervous system disorders
Headache
16.7%
2/12 • 23 days

Additional Information

Hadigan, Colleen

Clinical Center

Phone: +1 301 594 5754

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place