Trial Outcomes & Findings for Automated Method for Breast Cancer Detection (NCT NCT03863522)

NCT ID: NCT03863522

Last Updated: 2021-11-19

Results Overview

Number of participants in whom DNA methylation profile of 10 genes in the Cancer Detection cartridge correlates with diagnosis based on the gold standard of histopathology of the core biopsy or resected sample.

Recruitment status

TERMINATED

Study phase

NA

Target enrollment

116 participants

Primary outcome timeframe

up to 2 years

Results posted on

2021-11-19

Participant Flow

Participant milestones

Participant milestones
Measure
Fine Needle Aspiration
Participants will receive fine needle aspiration (FNA) later read by the cytopathologist and the cartridge. All patients will receive standard of care (Ultrasound-guided core needle biopsy and diagnosis). Fine needle aspiration: Fine needle aspiration is a type of biopsy procedure to diagnose suspicious lesions. After giving numbing medicine, a thin needle is inserted into the breast to obtain a sample. This procedure is followed by the standard of care and will last about 10 minutes. The sample will be analyzed by a diagnostic cartridge and by the cytopathologist. Cancer Detection cartridge: The Cancer Detection cartridge has the ability to measure methylated gene changes in breast cells. The device will not be in contact with the patients. The FNA samples will be smeared on a slide which will be placed into the cartridge for analysis.
Overall Study
STARTED
116
Overall Study
COMPLETED
116
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Automated Method for Breast Cancer Detection

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Fine Needle Aspiration
n=116 Participants
Participants will receive fine needle aspiration (FNA) later read by the cytopathologist and the cartridge. All patients will receive standard of care (Ultrasound-guided core needle biopsy and diagnosis). Fine needle aspiration: Fine needle aspiration is a type of biopsy procedure to diagnose suspicious lesions. After giving numbing medicine, a thin needle is inserted into the breast to obtain a sample. This procedure is followed by the standard of care and will last about 10 minutes. The sample will be analyzed by a diagnostic cartridge and by the cytopathologist. Cancer Detection cartridge: The Cancer Detection cartridge has the ability to measure methylated gene changes in breast cells. The device will not be in contact with the patients. The FNA samples will be smeared on a slide which will be placed into the cartridge for analysis.
Age, Continuous
48 years
n=5 Participants
Sex: Female, Male
Female
116 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
1 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
71 Participants
n=5 Participants
Race (NIH/OMB)
White
40 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
3 Participants
n=5 Participants
Region of Enrollment
United States
62 Participants
n=5 Participants
Region of Enrollment
South Africa
54 Participants
n=5 Participants

PRIMARY outcome

Timeframe: up to 2 years

Population: Inadequate cell counts prevented analysis of samples. Data was not generated.

Number of participants in whom DNA methylation profile of 10 genes in the Cancer Detection cartridge correlates with diagnosis based on the gold standard of histopathology of the core biopsy or resected sample.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: up to 2 years

Population: Inadequate cell counts prevented analysis of samples. Data was not generated.

Number of participants in whom DNA methylation profile of 10 genes as determined in the Cancer Detection cartridge correlates with FNA cytology

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: up to 2 years

Population: Inadequate cell counts prevented analysis of samples. Data was not generated.

Number of participants in whom gene-methylation prediction of FNA in the Cancer Detection cartridge is malignant or benign when compared to gene-methylation prediction of FNA using our known laboratory assay (QM-MSP)

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: up to 2 years

Population: Inadequate cell counts prevented analysis of samples. Data was not generated.

Number of participants in whom gene-methylation prediction of benign or malignant using our known laboratory assay (QM-MSP) matches for both the FNA sample and the core biopsy sample

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: up to 2 years

Population: Inadequate cell counts prevented analysis of samples. Data was not generated.

Number of participants with same expression of ER/PR/Her2 and Ki67 in FNAs of methylation positive breast lesions as with expression of ER/PR/Her2 and Ki67 in Formalin-Fixed Paraffin-Embedded (FFPE) sections of tumors using cartridge based quantitative evaluation

Outcome measures

Outcome data not reported

Adverse Events

Fine Needle Aspiration

Serious events: 0 serious events
Other events: 3 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Fine Needle Aspiration
n=62 participants at risk
Participants will receive fine needle aspiration (FNA) later read by the cytopathologist and the cartridge. All patients will receive standard of care (Ultrasound-guided core needle biopsy and diagnosis). Fine needle aspiration: Fine needle aspiration is a type of biopsy procedure to diagnose suspicious lesions. After giving numbing medicine, a thin needle is inserted into the breast to obtain a sample. This procedure is followed by the standard of care and will last about 10 minutes. The sample will be analyzed by a diagnostic cartridge and by the cytopathologist. Cancer Detection cartridge: The Cancer Detection cartridge has the ability to measure methylated gene changes in breast cells. The device will not be in contact with the patients. The FNA samples will be smeared on a slide which will be placed into the cartridge for analysis.
Injury, poisoning and procedural complications
Bruising
0.00%
0/62 • 2 weeks
The only intervention is FNA, which is immediately preceded by a core needle biopsy. Thus the cause, FNA vs core biopsy, of the AE will be impossible to determine. Via phone 1-2 days post-procedure and again 10-14 days post-procedure, patients were asked about any bruising and pain, but could report other symptoms if any. Please note only the 62 Johns Hopkins patients were called; the remaining 54 South African patients were not followed for AEs by this study team.
Injury, poisoning and procedural complications
Pain
4.8%
3/62 • Number of events 3 • 2 weeks
The only intervention is FNA, which is immediately preceded by a core needle biopsy. Thus the cause, FNA vs core biopsy, of the AE will be impossible to determine. Via phone 1-2 days post-procedure and again 10-14 days post-procedure, patients were asked about any bruising and pain, but could report other symptoms if any. Please note only the 62 Johns Hopkins patients were called; the remaining 54 South African patients were not followed for AEs by this study team.
Injury, poisoning and procedural complications
Other
0.00%
0/62 • 2 weeks
The only intervention is FNA, which is immediately preceded by a core needle biopsy. Thus the cause, FNA vs core biopsy, of the AE will be impossible to determine. Via phone 1-2 days post-procedure and again 10-14 days post-procedure, patients were asked about any bruising and pain, but could report other symptoms if any. Please note only the 62 Johns Hopkins patients were called; the remaining 54 South African patients were not followed for AEs by this study team.

Additional Information

Dr. Sara Sukumar

Johns Hopkins University

Phone: 4106142479

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place