Seminal Level of Clusterin Before Testicular Sperm Extraction

NCT ID: NCT03857828

Last Updated: 2020-12-21

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

88 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-12-16

Study Completion Date

2021-05-01

Brief Summary

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Measurement of clusterin level in the semen of infertile males undergoing testicular sperm extraction.

Detailed Description

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Clusterin, known as apolipoprotein J, sulphated glycoprotein-2 or testosterone - repressed prostate message-2.

It plays important roles in several pathophysiological processes, including tissue remodelling, lipid transport, reproduction, complement regulation and apoptotic cell death .

As clusterin expression is markedly upregulated in various normal and malignant tissues undergoing apoptosis, it has been regarded as a marker for cell death.

There is a conflicting findings concerning the relationship between elevated clusterin expression and enhanced induction of apoptosis; that is, clusterin has appeared to have a powerful anti-apoptotic function through a chaperone-like activity.

In addition to the anti-apoptotic activity, seminal clusterin was reported to promote a tolerogenic response to male antigens, thereby contributing to female tolerance to seminal antigens.

In the testis, clusterin is secreted by Sertoli cells into the fluid of the seminiferous epithelium and deposited onto the membranes of elongating spermatids and mature spermatozoa.

To date, however, there has been little information with respect to the functional roles of clusterin in the male reproductive tract under physiological conditions.

In particular, it remains controversial as to whether or not clusterin helps assist the normal spermatogenesis.

Nonobstructive azoospermia (NOA) males are characterised by impaired spermatogenesis.

Although NOA patients have impaired global spermatogenic function, focal areas of spermatogenesis may still exist in their testes.

Focal spermatogenesis could possibly be obtained by testicular sperm extraction (TESE) technique .

A number of factors have been suggested to be of predictive value for patients with a good chance to retrieve sperm cell such as testicular volume, serum FSH levels , serum total testosterone and serum inhibin B levels.

Conditions

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Azoospermia, Nonobstructive

Study Design

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Observational Model Type

OTHER

Study Time Perspective

PROSPECTIVE

Interventions

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semen sample

• seminal sample for measuring clusterin level.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Infertile males , Azoospermia undergoing TESE as a preliminary step for ICSI .
* Age: 20-50 year

Exclusion Criteria

* males \<20 years and \>50years
* Cryptorchidism .
* Testicular Agenesis and testicular atrophy .
Minimum Eligible Age

20 Years

Maximum Eligible Age

50 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Assiut University

OTHER

Sponsor Role lead

Responsible Party

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Azza Sheshaey Hassan Abdelfadel Ahmad

Principal investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Central Contacts

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Azza Sheshaey, M.B.B.CH

Role: CONTACT

Phone: 01096906812

Email: [email protected]

Emad Eldien Kamal, Ph.D

Role: CONTACT

Phone: 01004026100

Other Identifiers

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CLu before TESE

Identifier Type: -

Identifier Source: org_study_id