Clinical Trial Multi-analyte Blood Test

NCT ID: NCT03694600

Last Updated: 2024-05-31

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

1900 participants

Study Classification

OBSERVATIONAL

Study Start Date

2019-02-04

Study Completion Date

2024-06-30

Brief Summary

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This is a clinical trial designed to evaluate the performance of a multi-analyte blood test alone to ultrasound alone for the detection of HCC within a population that is at high risk for HCC due to liver cirrhosis.

Detailed Description

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This multi-site, prospective study is designed to compare the sensitivity and specificity of a multi-analyte blood test alone to ultrasound alone for the detection of HCC within a population at high risk of HCC due to liver cirrhosis. Subjects will be enrolled until the pre-determined number of subjects are enrolled.

Subjects at high risk for developing HCC due to liver cirrhosis and who are eligible for liver cancer surveillance as determined by the patient's physician and who meet all inclusion and exclusion eligibility criteria as described in this protocol, will be invited to participate in this study. Subjects will then read, understand and sign the Informed Consent Form and the HIPAA Authorization Agreement for Medical Records Form.

For each subject upon enrollment, the following blood analytes will also be determined: creatinine, prothrombin time, bilirubin, blood platelet count, ALT, AST and ALP. The results of all clinical laboratory tests will be recorded by use of the subject's Case Report Form. Whole blood samples drawn for the multi-analyte blood test will be collected (according to the instructions provided with each sample collection kit) by using the multi-analyte Sample Collection, Stabilization and Shipping Kit, and shipped to a central LAM laboratory for testing. Samples will be assayed by laboratory technicians blinded to the results of any other testing. Within the same clinical visit as the blood draws (when possible), subjects will undergo conventional ultrasound to examine the liver. Every subject will then go on to diagnostic imaging by multiphasic MRI. The results of diagnostic imaging will primarily be scored by LI-RADS score and the number and size of any malignant lesions identified will be recorded. The images of all MRI, CT, or ultrasound will be saved and uploaded for evaluation by a blinded, centralized team of radiologists to confirm diagnosis. Any biopsy results or surgical pathology results that are generated for study subjects as part of current clinical practice will also be recorded.

Study procedures will consist of conventional ultrasound to examine the liver, providing blood samples for testing with the multi-analyte blood test and other conventional blood analytes, and diagnostic imaging by multiphasic MRI.

Upon enrolling in the study, subjects will commence the Initial Surveillance Visit (t=0 months). During the Initial Surveillance Visit, all enrolled subjects will undergo ultrasound to examine the liver and provide blood samples for the multi-analyte blood test and for determining conventional blood analytes. Every subject will then go on to diagnostic imaging by multiphasic MRI. The results of diagnostic imaging will primarily be scored by a Liver Reporting and Data System (LI-RADS) score. The data of all diagnostic imaging by MRI will be saved and uploaded for evaluation by a blinded, centralized team of radiologists, which will be used as the basis of the clinical truth for all subjects.

After the Initial Surveillance Visit (t=0 months), subjects with an indeterminant HCC finding by MRI (LI-RADS 3) will be recommended to up to three Follow-Up Visits (t=6 months, t=12 months, and t=18 months) to attempt to resolve the clinical truth for these subjects. Each Follow-Up Visit will consist of an ultrasound to examine the liver, providing blood samples for the multi-analyte blood test , as well as diagnostic imaging by multiphasic MRI.

Although not required by this clinical protocol, any biopsy or surgical pathology results, or any additional imaging (such as multiphasic CT) that are generated for study subjects as part of current clinical practice will also be recorded for each subject and the results shall be made available to the Sponsor if performed within 6 months of a scheduled Visit. Biopsy and surgical pathology results that indicate a malignancy will be used in place of diagnostic imaging as the clinical truth for each subject if performed within 6 months of a scheduled visit and prior to database lock.

All study-related procedures will occur during the Study Duration Period, which consists of the Initial Surveillance Visit for all subjects and up to three Follow-Up Visits for subjects with an initial indeterminant HCC finding (LI-RADS 3) by diagnostic imaging. After this Study Duration Period, no study related blood draws, imaging or procedures will occur for these subjects.

Conditions

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Liver Cirrhosis

Study Design

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Observational Model Type

OTHER

Study Time Perspective

PROSPECTIVE

Study Groups

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men or women between 21-84

Multi-analyte blood test alone versus ultrasound in subjects diagnosed with liver cirrhosis

Multi-analyte blood Test

Intervention Type DIAGNOSTIC_TEST

intended for the qualitative detection of DNA methylation profiles associated with hepatocellular carcinoma in cell-free DNA derived from patient whole blood specimens.

Interventions

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Multi-analyte blood Test

intended for the qualitative detection of DNA methylation profiles associated with hepatocellular carcinoma in cell-free DNA derived from patient whole blood specimens.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Subject is age 21 to 84 (inclusive)
* Subject is able to read, comprehend and sign the Informed Consent Document
* Subject is willing and able to undergo liver cancer surveillance by ultrasound and the multi-analyte blood Test
* Subject is able and willing to undergo diagnostic imaging by multiphasic MRI with contrast according to the Study Protocol
* Subject has been diagnosed with liver cirrhosis by one or more of the following methods:

1. Clinical diagnosis by blood analytes (APRI ≥ 1.5 or Bonacini cirrhosis discriminant score ≥ 8 or Lok index \> 0.5)
2. Ultrasound and Elastography \> 12.5 kPa (Vibration-controlled transient elastography, Point shear wave elastography, Two-dimensional shear wave ultrasound and transient elastography or Magnetic Resonance Elastography)
3. Diagnostic imaging by CT or MRI (liver cirrhosis indicated on radiology report)
4. Liver biopsy (liver cirrhosis indicated on pathology report)

Exclusion Criteria

* The study investigator deems the subject's participation to be unsafe due to an underlying medical condition
* Subject has previously been diagnosed with a primary liver cancer or a non-liver cancer that has metastasized
* Subject has previously submitted a blood sample to Helio Health (HELIO) through a separate clinical protocol
* Subject is unable or unwilling to submit blood samples for testing, undergo ultrasound or undergo diagnostic imaging by multiphasic MRI with contrast as required for the Study Protocol
* Subject has any internal metallic device or fragment, including but not limited to: A pacemaker, artificial joint or valve, surgical clips, pins, plates, screws, wire mesh or shrapnel
* It is unsafe for the subject to receive an MRI contrast dye due to pregnancy or severe kidney disease
* Subject would not routinely be recommended for HCC surveillance
* Subject received an abdominal ultrasound or diagnostic imaging by MRI or CT to image the liver within the past 5 months
Minimum Eligible Age

21 Years

Maximum Eligible Age

84 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Helio Genomics

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Taggart, PhD

Role: STUDY_DIRECTOR

Helio Genomics

Locations

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AZ Liver Health (Chandler)

Chandler, Arizona, United States

Site Status

AZ Liver Health (Glendale, Peoria)

Peoria, Arizona, United States

Site Status

AZ Liver Health (Tucson)

Tucson, Arizona, United States

Site Status

Banner University Hospital (University of Arizona Tucson)

Tucson, Arizona, United States

Site Status

Alliance Research Centers

Irvine, California, United States

Site Status

University of South California

Los Angeles, California, United States

Site Status

Cedars-Sinai Medical Center

Los Angeles, California, United States

Site Status

Stanford University

Palo Alto, California, United States

Site Status

University of Colorado Hospital - Anschutz Cancer Pavilion

Aurora, Colorado, United States

Site Status

Covenant Metabolic LLC

Fort Myers, Florida, United States

Site Status

University of Florida Clinical and Translational Science Institute

Gainesville, Florida, United States

Site Status

University of Miami - Schiff Center for Liver Diseases

Miami, Florida, United States

Site Status

Gastroenterology Group of Naples

Naples, Florida, United States

Site Status

Covenant Metabolics LLC

Sarasota, Florida, United States

Site Status

Atlanta Gastroenterology

Atlanta, Georgia, United States

Site Status

Piedmont Research Institute

Atlanta, Georgia, United States

Site Status

Loyola University Medical Center

Maywood, Illinois, United States

Site Status

St Luke's Hospital of Kansas City

Kansas City, Kansas, United States

Site Status

University of Louisville

Louisville, Kentucky, United States

Site Status

Tulane University Health Sciences Center

New Orleans, Louisiana, United States

Site Status

Ochsner Clinic Foundation

New Orleans, Louisiana, United States

Site Status

Louisiana Research Center, LLC

Shreveport, Louisiana, United States

Site Status

Kansas City Research Institute (KCRI)

Kansas City, Missouri, United States

Site Status

PMG Research of Winston-Salem

Winston-Salem, North Carolina, United States

Site Status

University of Cincinnati College of Medicine

Cincinnati, Ohio, United States

Site Status

Gastro One in Memphis

Memphis, Tennessee, United States

Site Status

Quality Medical

Nashville, Tennessee, United States

Site Status

Liver Center of Texas

Dallas, Texas, United States

Site Status

Methodist Dallas Medical Center

Dallas, Texas, United States

Site Status

SouthTexas Research Institute

Edinburg, Texas, United States

Site Status

Texas Gastro Clinic

El Paso, Texas, United States

Site Status

Konkord Research

Houston, Texas, United States

Site Status

Texas Liver Institute - American Research Corp.

San Antonio, Texas, United States

Site Status

Impact Research Institute

Waco, Texas, United States

Site Status

Digestive & Liver Disease Specialists (DLDS) - Norfolk

Norfolk, Virginia, United States

Site Status

VA Richmond

Richmond, Virginia, United States

Site Status

Harborview Medical Center (University of Washington)

Seattle, Washington, United States

Site Status

Liver Institute Northwest

Seattle, Washington, United States

Site Status

Countries

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United States

Other Identifiers

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LAM-2018-01

Identifier Type: -

Identifier Source: org_study_id

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