Non Invasive Prenatal Test of Rare Genetic Diseases: Application to Rare Intellectual Disabilities

NCT ID: NCT03688594

Last Updated: 2018-09-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-05-22

Study Completion Date

2019-05-23

Brief Summary

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The aim of this study is to evaluate performances of a NIPT test based onto the study of the maternal blood to search known genetic mutations already detected in the family and potentially inherited by the fetus. This test will avoid an invasive prenatal diagnosis in those families with a known genetic risk.

The performance of this test will be evaluated in terms of sensitivity and specificity with an adapted statistic model.

Secondary objectives of the protocol are

* To adapt NIPT to small DNA quantity (5-50 ng)
* To adapt bioinformatics pipeline to low rate of mosaicism
* To develop a tool to quantify the fetal fraction
* To evaluate the robustness of the method

This test is based onto capture and high throw put sequencing adapted to cell free plasmatic DNA of pregnant women in order to detect point mutation present in her fetus. This approach has been previously described for others clinical applications such as liquid biopsy in cancers but not for NIPT analysis.

Detailed Description

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Conditions

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Genetic Disorders in Pregnancy

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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couple : man and pregnant women

Group Type EXPERIMENTAL

NIPT Test

Intervention Type DIAGNOSTIC_TEST

This test is based onto capture and high throw put sequencing adapted to cell free plasmatic DNA of pregnant women in order to detect point mutation present in her fetus. This approach has been previously described for others clinical applications such as liquid biopsy in cancers but not for NIPT analysis.

Interventions

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NIPT Test

This test is based onto capture and high throw put sequencing adapted to cell free plasmatic DNA of pregnant women in order to detect point mutation present in her fetus. This approach has been previously described for others clinical applications such as liquid biopsy in cancers but not for NIPT analysis.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Couple (father, mother) \> 18 ans
* Pregnant woman (\> 12-15 weeks of gestation) with a fetal sampling needed in standard care.
* informed consent obtained
* couple affiliated to the social insurance in France

Exclusion Criteria

* DNA extraction failure
* Absence of informed consent
* Father or mother placed under judicial protection or under guardianship or tutorship
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Strasbourg, France

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Hôpitaux Universitaires de Strasbourg

Strasbourg, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Bénédicte GERARD, PharmD, PhD

Role: CONTACT

Phone: 03 69 55 07 77

Email: [email protected]

Facility Contacts

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bénédicte GERARD, PharmD, PhD

Role: primary

Other Identifiers

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6792

Identifier Type: -

Identifier Source: org_study_id