Designing Biofunctional Dairy Foods: Matrix Structure of Dairy Products in Relation to Lipaemia

NCT ID: NCT03656367

Last Updated: 2021-11-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

25 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-09-10

Study Completion Date

2019-03-21

Brief Summary

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The overall aim of this study is to investigate how dairy products with different structures and textures affect lipid absorption kinetics in the acute postprandial period via blood lipid biochemistry.

The study will be conducted as a randomized acute cross-over meal study. Apparently healthy men will be recruited in the study. They will on 4 test days consume the 4 dairy products and blood will be drawn the following 8 hours.

Detailed Description

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The overall aim of this study is to investigate how dairy products with similar nutrient content but different structures and textures affect lipid absorption kinetics in the acute postprandial period via blood lipid biochemistry, as well as nuclear magnetic resonance (NMR) and liquid chromatography mass spectrometry (LCMS)-based metabolomics.

The study will be conducted as a randomized acute cross-over meal study. On four different test days (with a minimum of 2 weeks washout period) four dairy products with similar nutrient composition but different structures or textures will be tested. During each meal test a single dairy product (+ bread and water) is served and postprandial blood samples are collected the following 8 hours. Subjective appetite measurements (VAS) are filled in during the 8-hour period and an ad libitum meal is served in the end of the day.

25 apparently healthy men (weight stable, 18-40 years, 18.5-24.9 kg/m2) will be recruited.

Conditions

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Lipaemia

Keywords

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glycaemia insulinemia lipaemia appetite

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Outcome Assessors
Due to the characteristics of the study design, blinding of project personnel and participants is not possible. However, statistical analyses of the results will be performed in a blinded fashion.

Study Groups

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All subjects

Cross-over study (all subjects receive all interventions)

* Cheddar cheese
* Blended and homogenized cheddar cheese
* An analog milk
* An analog cheese

Group Type EXPERIMENTAL

Cheddar cheese

Intervention Type DIETARY_SUPPLEMENT

The reference product is a commercial matured cheddar cheese

Blended and homogenized cheddar cheese

Intervention Type DIETARY_SUPPLEMENT

A blended and homogenized cheddar cheese (a spread) which, compared to the reference, has a minimal protein network and has smaller fat droplet

An analog milk

Intervention Type DIETARY_SUPPLEMENT

An anolog milk (i.e. milk with a cheese-like nutritional composition) which, compared to the reference, does not contain a protein network

An analog cheese

Intervention Type DIETARY_SUPPLEMENT

This analog cheese (a gel/yoghurt) is produced from the analog milk which, compared to the reference, has a protein network, however it is a network that differs from the reference

Healthy subjects only (subgroup)

If any subjects (against our hypothesis) have indices of metabolic syndrome i.e. raised fasting glucose or TG concentration, secondary analyses will be conducted to assess results without these subjects.

All subjects receive all interventions)

* Cheddar cheese
* Blended and homogenized cheddar cheese
* An analog milk
* An analog cheese

Group Type OTHER

Cheddar cheese

Intervention Type DIETARY_SUPPLEMENT

The reference product is a commercial matured cheddar cheese

Blended and homogenized cheddar cheese

Intervention Type DIETARY_SUPPLEMENT

A blended and homogenized cheddar cheese (a spread) which, compared to the reference, has a minimal protein network and has smaller fat droplet

An analog milk

Intervention Type DIETARY_SUPPLEMENT

An anolog milk (i.e. milk with a cheese-like nutritional composition) which, compared to the reference, does not contain a protein network

An analog cheese

Intervention Type DIETARY_SUPPLEMENT

This analog cheese (a gel/yoghurt) is produced from the analog milk which, compared to the reference, has a protein network, however it is a network that differs from the reference

Interventions

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Cheddar cheese

The reference product is a commercial matured cheddar cheese

Intervention Type DIETARY_SUPPLEMENT

Blended and homogenized cheddar cheese

A blended and homogenized cheddar cheese (a spread) which, compared to the reference, has a minimal protein network and has smaller fat droplet

Intervention Type DIETARY_SUPPLEMENT

An analog milk

An anolog milk (i.e. milk with a cheese-like nutritional composition) which, compared to the reference, does not contain a protein network

Intervention Type DIETARY_SUPPLEMENT

An analog cheese

This analog cheese (a gel/yoghurt) is produced from the analog milk which, compared to the reference, has a protein network, however it is a network that differs from the reference

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Apparently healthy men
* 18-40 years of age
* Body mass index (BMI): 18.5-24.9 kg/m\^2
* Non-smoker
* Haemoglobin concentration ≥8.4 mmol/L

Exclusion Criteria

* Dislike for dairy products such as milk, cheese and yoghurt
* Weight change \>3 kg 2 months prior to study
* Any other blood donation \< 3 month prior to study and during study, than the blood donation that is included in this study
* Intensive physical training (\>10 hours of per week)
* Self-reported eating disorders or irregular eating schedules (e.g. skipping breakfast) and uncommon diets (vegetarians, vegans etc.)
* Alcohol intake above the recommendation from the Danish Health and Medicines Authority (\>21 units of alcohol per week)
* Night- or shift work
* Food intolerance and allergies related to test products e.g. lactose and gluten
* Dietary supplements, if not taken on a regular basis
* Currently use, or use within previously 3 months of prescription medication, except non-prescription primary analgetica (NSAID and paracetamol) to relieve e.g. head ache and stomach pain, and glucocorticoider for topical use e.g. in ointment and creams.
* Chronic diseases e.g. cancer within the past 5 years (except adequately-treated localized basal cell skin cancer), asthma, back pain, thyroid disease, heart disease, diabetes, inflammation
* Disorders: neurological, sleep, diagnosed psychiatric disorder, and gastro intestinal and liver disorders.
* Surgical treatment of obesity and abdominal surgery
* Inability, physically or mental, to comply with the procedures required by the study protocol as evaluated by the daily study manager, principal investigator or clinical responsible
* Subject's general condition contraindicates continuing the study as evaluated by the daily study manager, principal investigator or clinical responsible
* Simultaneous participation in other clinical intervention studies
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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University of Aarhus

OTHER

Sponsor Role collaborator

Arla Foods

INDUSTRY

Sponsor Role collaborator

Anne Birgitte Raben

OTHER

Sponsor Role lead

Responsible Party

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Anne Birgitte Raben

professor

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Anne B Raben, prof

Role: PRINCIPAL_INVESTIGATOR

University of Copenhagen

Locations

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Department of Nutrition, Exercise and Sports

Frederiksberg, , Denmark

Site Status

Countries

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Denmark

References

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Kjolbaek L, Schmidt JM, Rouy E, Jensen KJ, Astrup A, Bertram HC, Hammershoj M, Raben A. Matrix structure of dairy products results in different postprandial lipid responses: a randomized crossover trial. Am J Clin Nutr. 2021 Nov 8;114(5):1729-1742. doi: 10.1093/ajcn/nqab220.

Reference Type RESULT
PMID: 34477812 (View on PubMed)

Thogersen R, Lindahl IEI, Khakimov B, Kjolbaek L, Juhl Jensen K, Astrup A, Hammershoj M, Raben A, Bertram HC. Progression of Postprandial Blood Plasma Phospholipids Following Acute Intake of Different Dairy Matrices: A Randomized Crossover Trial. Metabolites. 2021 Jul 14;11(7):454. doi: 10.3390/metabo11070454.

Reference Type RESULT
PMID: 34357348 (View on PubMed)

Schmidt JM, Kjolbaek L, Jensen KJ, Rouy E, Bertram HC, Larsen T, Raben A, Astrup A, Hammershoj M. Influence of type of dairy matrix micro- and macrostructure on in vitro lipid digestion. Food Funct. 2020 Jun 24;11(6):4960-4972. doi: 10.1039/d0fo00785d.

Reference Type RESULT
PMID: 32500911 (View on PubMed)

Other Identifiers

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B346 (H-18024447)

Identifier Type: -

Identifier Source: org_study_id