Neoantigen DNA Vaccine in Combination With Nivolumab/Ipilimumab and PROSTVAC in Metastatic Hormone-Sensitive Prostate Cancer

NCT ID: NCT03532217

Last Updated: 2022-08-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

19 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-09-14

Study Completion Date

2022-07-25

Brief Summary

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This study study aims to elucidate the immune responses to a shared antigen vaccine (PROSTVAC) and tumor specific antigens generated DNA vaccine in combination with checkpoint blockade using nivolumab (anti-PD-1), and ipilimumab (anti-CTLA-4). Additionally, the investigators will study the impact of the combination immunotherapy on peripheral T cell activation, as well as immune response in the tumor microenvironment. Finally, the investigators will evaluate the safety and tolerability to this novel personalized immunotherapy in combination with checkpoint blockade.

Detailed Description

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Conditions

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Metastatic Hormone-Sensitive Prostate Cancer

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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PROSTVAC/Ipilimumab/Nivolumab/Neoantigen DNA vaccine

* Within 60 days after the last chemo, patients will start a priming dose of PROSTVAC-V, and subsequent doses of PROSTVAC- F (weeks 0, 2, 5, 8, 11, 14, and 17). During "Treatment A" phase, subjects should receive nivolumab intravenously on Day 1 of each cycle every 3 weeks for 6 doses. Ipilimumab on Day 1 of each cycle every 3 weeks for 2 doses.
* Patients will then receive a neoantigen DNA vaccine with continuous nivolumab treatment. The vaccine will be administered starting approximately week 21 by intramuscular injection for a total of 6 treatments every 28 days +/-7 days. Each DNA vaccination will be 4 mg vaccine administered intramuscularly using a TriGrid electroporation device. Patients will receive nivolumab at 480 mg every 28 days concurrently with neoantigen DNA vaccine. This is Treatment B. In the event the DNA vaccine production is delayed, patients will receive single agent nivolumab every 4 weeks beginning week 21 until the vaccine is ready

Group Type EXPERIMENTAL

PROSTVAC-V

Intervention Type BIOLOGICAL

-Replication-competent vaccinia virus which has been engineered to encode the sequences for a modified human prostate-specific antigen (PSA) and a triad of co-stimulatory molecules (TRICOM)

PROSTVAC-F

Intervention Type BIOLOGICAL

-Fowlpox virus which does not replicate in human cells and has been engineered to encode the same sequences present in PROSTVAC-V.

Nivolumab

Intervention Type DRUG

-Nivolumab is a human monoclonal antibody (mAb)

Ipilimumab

Intervention Type DRUG

-Ipilimumab is a mAb blocking the inhibitory signal mediated by cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4), a protein receptor that downregulates the immune system.

Neoantigen DNA vaccine

Intervention Type BIOLOGICAL

Each DNA vaccination will be 1 mL vaccine administered intramuscularly. At each vaccination time point, patients will receive two injections at separate sites.

TriGrid Delivery System

Intervention Type DEVICE

-Electroporation device

Tumor biopsy

Intervention Type PROCEDURE

-Pre-treatment, post-treatment A (optional), and end of treatment

Peripheral blood

Intervention Type PROCEDURE

-At the time of pre-treatment biopsy, mid-treatment of chemo-ADT, at time of enrollment (prior to POSTVAC administration), mid-treatment A, mid-treatment B (multiple)

Fecal samples

Intervention Type PROCEDURE

-Post chemo/pre-treatment A, post-treatment A, pre-treatment B, post-treatment B

Leukapheresis

Intervention Type PROCEDURE

* Post-treatment A/pre-treatment B
* Mid-Treatment B, after Cycle 9 Day 1 and prior to Cycle 10 Day 1
* End of treatment

Interventions

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PROSTVAC-V

-Replication-competent vaccinia virus which has been engineered to encode the sequences for a modified human prostate-specific antigen (PSA) and a triad of co-stimulatory molecules (TRICOM)

Intervention Type BIOLOGICAL

PROSTVAC-F

-Fowlpox virus which does not replicate in human cells and has been engineered to encode the same sequences present in PROSTVAC-V.

Intervention Type BIOLOGICAL

Nivolumab

-Nivolumab is a human monoclonal antibody (mAb)

Intervention Type DRUG

Ipilimumab

-Ipilimumab is a mAb blocking the inhibitory signal mediated by cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4), a protein receptor that downregulates the immune system.

Intervention Type DRUG

Neoantigen DNA vaccine

Each DNA vaccination will be 1 mL vaccine administered intramuscularly. At each vaccination time point, patients will receive two injections at separate sites.

Intervention Type BIOLOGICAL

TriGrid Delivery System

-Electroporation device

Intervention Type DEVICE

Tumor biopsy

-Pre-treatment, post-treatment A (optional), and end of treatment

Intervention Type PROCEDURE

Peripheral blood

-At the time of pre-treatment biopsy, mid-treatment of chemo-ADT, at time of enrollment (prior to POSTVAC administration), mid-treatment A, mid-treatment B (multiple)

Intervention Type PROCEDURE

Fecal samples

-Post chemo/pre-treatment A, post-treatment A, pre-treatment B, post-treatment B

Intervention Type PROCEDURE

Leukapheresis

* Post-treatment A/pre-treatment B
* Mid-Treatment B, after Cycle 9 Day 1 and prior to Cycle 10 Day 1
* End of treatment

Intervention Type PROCEDURE

Other Intervention Names

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TDS-IM

Eligibility Criteria

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Inclusion Criteria

* Histologically confirmed adenocarcinoma of the prostate.
* High risk/volume metastatic disease, as defined by 4 or more sites of disease or the presence of visceral metastases.
* Must have completed an adequate course of chemo-hormonal, first line therapy for metastatic hormone-sensitive prostate cancer, as determined by the investigator. Patients must remain on stable dose of ADT with castrate levels of testosterone (defined as testosterone \< 50 ng/dL)
* At least 18 years of age.
* PSA may be undetectable after initial chemo-ADT.
* ECOG performance status ≤ 2
* Normal bone marrow and organ function as defined below:

* Leukocytes ≥ 2,000/ul
* Absolute neutrophil count ≥ 1,500/ul
* Platelets ≥ 100,000/ul
* Hemoglobin ≥ 9.0 g/ul
* Total bilirubin ≤ 1.5 x ULN (except subjects with Gilbert's syndrome who must have a total bilirubin level of ≤ 3.0 ULN)
* AST(SGOT) ≤ 3.0 x ULN
* ALT(SGPT) ≤ 3.0 x ULN
* Creatinine ≤ 1.5 x ULN OR calculated creatinine clearance ≥ 40 mL/min using the Cockcroft-Gault formula
* Ability to understand and willingness to sign an IRB approved written informed consent document (or that of legally authorized representative, if applicable).
* Must have a biopsy of a metastatic site of disease (may be archival) available and adequate for evaluation and determination of neoantigens by genomic analyses.
* Must have all AEs resolved to baseline prior to chemo-ADT, or if treatment related, resolved to grade 1 prior to enrollment.

Exclusion Criteria

* Significant small cell or neuroendocrine component or histology, as determined by the institution's reading pathologist.
* Progression of disease as defined by a rising PSA (3 sequential values, at least 1 week apart) or radiographic progression based on RECIST1.1 or PCWG3 criteria.
* Prior treatment with a checkpoint inhibitor, neoantigen vaccine, or PROSTVAC.
* Participants with active, known or suspected autoimmune disease. Participants with type I diabetes mellitus, hypothyroidism only requiring hormone replacement, skin disorders (such as vitiligo, psoriasis, or alopecia) not requiring systemic treatment are permitted to enroll.
* Diagnosis of atopic dermatitis or other active exfoliative skin condition
* History of concurrent second cancers requiring active, ongoing systemic treatment
* Currently receiving any other investigational agents.
* Known brain metastases. Prostate cancer patients with known brain metastases must be excluded from this clinical trial because of their poor prognosis, and because they often develop progressive neurologic dysfunction that would confound the evaluation of neurologic and other adverse events.
* A history of allergic reactions attributed to compounds of similar chemical or biologic composition to anti-PD-1, anti-CTLA-4, Prostvac-VF Tricom, DNA vaccines, or other agents used in the study.
* Prior allergy or significant systemic reaction to vaccinia.
* Prior reactions to monoclonal antibodies.
* Received hematopoietic stem cell transplant \< 24 months prior to enrollment to this study, or received hematopoietic stem cell transplant ≥ 24 months prior to enrollment to this study but has graft-versus-host disease or disease relapse.
* Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, clinically significant congestive heart failure (NYHA Class III, IV), unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that in the opinion of the investigator would limit compliance with study requirements.
* Immunosuppressed status (e.g. HIV/AIDS, active HCV/HBV, high dose systemic steroids, etc.) as determined by the investigator; topical or inhaled steroids are acceptable.
* History of syncopal or vasovagal episode as determined by medical record and history in the 12 month period prior to first vaccination administration.
* Individuals in whom a skinfold measurement of the cutaneous and subcutaneous tissue for eligible injection sites (left and right medial deltoid region) exceeds 40 mm.
* Individuals in whom the ability to observe possible local reactions at the eligible injection sites (deltoid region) is, in the opinion of the investigator, unacceptably obscured due to a physical condition or permanent body art.
* Therapeutic or traumatic metal implant in the skin or muscle of either deltoid region.
* Any chronic or active neurologic disorder, including seizures and epilepsy, excluding a single febrile seizure as a child.
* Current use of any electronic stimulation device, such as cardiac demand pacemakers, automatic implantable cardiac defibrillator, nerve stimulators, or deep brain stimulators.
Minimum Eligible Age

18 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Bristol-Myers Squibb

INDUSTRY

Sponsor Role collaborator

Prostate Cancer Foundation

OTHER

Sponsor Role collaborator

The Foundation for Barnes-Jewish Hospital

OTHER

Sponsor Role collaborator

Bavarian Nordic

INDUSTRY

Sponsor Role collaborator

Washington University School of Medicine

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Russell Pachynski, M.D.

Role: PRINCIPAL_INVESTIGATOR

Washington University School of Medicine

Locations

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Washington University School of Medicine

St Louis, Missouri, United States

Site Status

Countries

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United States

Related Links

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http://www.siteman.wustl.edu

Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine

Other Identifiers

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CA209-9MW

Identifier Type: OTHER

Identifier Source: secondary_id

201808043

Identifier Type: -

Identifier Source: org_study_id

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