Streptoccus Pseudopneumoniae atAssiut UniversityHospitals

NCT ID: NCT03528759

Last Updated: 2019-02-05

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 100 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2019-07-01
• Study Completion Date: 2019-08-03

Brief Summary

Streptococcus pseudopneumoniae as suggested by DNA-DNA homology studies is recorded as a species of the Streptococcus mitis/oralis members of viridans group Streptococci; they have some similar features to the Streptococcus mitis/oralis members of viridans Streptococcus pneumoniae

Detailed Description

principle characteristics of Streptococcus. pseudopneumoniae are the lacking of pneumococcal capsule, bile insolubility, the indeterminate sensitivity Or resistance to optochin after incubation in Carbon dioxide (5%), while sensitivity to it after incubation in ambient air Streptococcus . pseudopneumoniae was firstly described in sputum samples of patients with lower respiratory tract infection, particularly those with Chronic Obstructive PulmonaryDiseases pseudopneumoniae is usually misidentified in our laboratories as we usually rely on optochin sensitivity testing with 5% Carbon dioxide detection of Streptococcus. pneumoniae and miss the incubation in ambient air which is the key point for identification of Streptococcus. pseudopneumoniae. Biochemical reactions are usually used to differentiate typical pneumoniae from Streptococc Streptococcus . pseudopneumoniae and other related Streptococci; several molecular techniques have also been used There are several polymerase chain reaction based assays that target specific pneumococcal virulence factors for the typical Streptococc Streptococcus . pneumoniae, such as autolysin A which is the major pneumococcal autolysin and the capsular polysaccharide biosynthesis gene A which is a conservedpneumococcal capsular polysaccharide gene , also aliB-like ORF2 (a gene that usually found in the capsular region of non-capsulated pneumococci; these genetic markers have been used to differentiate S. pneumoniae from other related species.

Conditions

Pneumonia; Streptococcus Infection

Study Design

• Observational Model Type: OTHER
• Study Time Perspective: CROSS_SECTIONAL

Interventions

phenotypic and genotypic Method

Intervention Type: DIAGNOSTIC_TEST

Other Intervention Names

Bile solubility test

Eligibility Criteria

Inclusion Criteria

• All patients showing signs of respiratory tract infection

• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Zeinab Abdelfadeel

Principle Investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

References

Raddaoui A, Simoes AS, Baaboura R, Felix S, Achour W, Ben Othman T, Bejaoui M, Sa-Leao R, Ben Hassen A. Serotype Distribution, Antibiotic Resistance and Clonality of Streptococcus pneumoniae Isolated from Immunocompromised Patients in Tunisia. PLoS One. 2015 Oct 13;10(10):e0140390. doi: 10.1371/journal.pone.0140390. eCollection 2015.

Reference Type: RESULT

PMID: 26461259 (View on PubMed)

Charalambous BM. Identifying divergent streptococcal strains using pherotype: implications for pathogen emergence and epidemiology. Future Microbiol. 2012 Dec;7(12):1349-54. doi: 10.2217/fmb.12.114.

Reference Type: RESULT

PMID: 23231485 (View on PubMed)

Leung MH, Ling CL, Ciesielczuk H, Lockwood J, Thurston S, Charalambous BM, Gillespie SH. Streptococcus pseudopneumoniae identification by pherotype: a method to assist understanding of a potentially emerging or overlooked pathogen. J Clin Microbiol. 2012 May;50(5):1684-90. doi: 10.1128/JCM.00131-12. Epub 2012 Feb 29.

Reference Type: RESULT

PMID: 22378913 (View on PubMed)

Other Identifiers

ZIS

Identifier Type: -

Identifier Source: org_study_id