The Neuroinflammatory Response and Biomarkers in Acute Traumatic Spinal Cord Injury

NCT ID: NCT03505463

Last Updated: 2020-11-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

30 participants

Study Classification

OBSERVATIONAL

Study Start Date

2018-05-01

Study Completion Date

2020-12-01

Brief Summary

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The study is a prospective cohort study designed to assess the diagnostic and prognostic potential of biomarker measurement in acute traumatic spinal cord injury (TSCI), and to examine the neuroinflammatory response to acute TSCI.

Detailed Description

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TSCI is an incurable condition with devastating consequences. The physical, psychosocial and financial implications in addition to a variable recovery and an uncertain prognosis have a profound impact on quality of life.The pathophysiology of TSCI is dual consisting of a primary injury and a secondary injury. The immediate trauma to the spinal cord and nerve roots causes the primary injury, while a prolonged cascade of events causes the secondary injury. The neuroinflammatory response is considered to be an important event. Current clinical measures to evaluate acute TSCI consist of clinical examination and routine imaging modalities. These measurements are inaccurate to assess injury severity, prognosis, and therapeutic efficacy, especially in the early acute phase. Biomarker measurement may provide a more accurate measure.

This study aim to assess the diagnostic and prognostic potential of biomarker measurement in acute TSCI, and to examine the neuroinflammatory response to acute TSCI. To achieve this, immune cells, cytokines, autoantibodies and structural proteins will be analysed in blood and cerebrospinal fluid by Flow Cytometry and assay techniques. These analyses will be correlated to clinical outcome assessed according to international standards.

Hopefully, this will contribute with new knowledge of the neuroinflammatory response and biomarkers in acute TSCI, and provide the needed knowledge for conducting futures trials in novel therapeutic interventions.

Conditions

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Spinal Cord Injury, Acute

Keywords

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Spinal cord injury Biomarkers Inflammation Trauma

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Injured participants

Blood samples

Intervention Type OTHER

Three blood samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

One blood sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Cerebrospinal fluid sample

Intervention Type OTHER

Three cerebrospinal fluid samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

The collected samples will be analysed by Flow Cytometry and assay techniques. One cerebrospinal fluid sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Clinical examination

Intervention Type OTHER

Clinical examinations will be performed at \<72 hours, 7 days and 365 days post-injury.

Healthy participants

Blood samples

Intervention Type OTHER

Three blood samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

One blood sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Cerebrospinal fluid sample

Intervention Type OTHER

Three cerebrospinal fluid samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

The collected samples will be analysed by Flow Cytometry and assay techniques. One cerebrospinal fluid sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Interventions

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Blood samples

Three blood samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

One blood sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Intervention Type OTHER

Cerebrospinal fluid sample

Three cerebrospinal fluid samples will be collected from the injured participants. The assessment points are \<72 hours, 7 days and 365 days post-injury.

The collected samples will be analysed by Flow Cytometry and assay techniques. One cerebrospinal fluid sample will be collected from the healthy participants. The collection will be performed as part of clinical diagnostic at the Department of Neurology at Aarhus University Hospital.

Laboratory analysis of the collected samples comprise Flow Cytometry and assay techniques.

Intervention Type OTHER

Clinical examination

Clinical examinations will be performed at \<72 hours, 7 days and 365 days post-injury.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Traumatic spinal cord injury (Bony level C1-L1)
* ISNCSCI score A-C upon inclusion.
* Age \>18-70 years.

Exclusion Criteria

* Prior major spine surgery in the traumatised location.
* Major co-morbidities or co-injuries.
* Penetrating spinal cord injury.
* \>72 hours from injury to sample collection.
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Aarhus University Hospital

OTHER

Sponsor Role collaborator

Spinal Cord Injury Centre of Western Denmark

OTHER

Sponsor Role collaborator

University of Aarhus

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Aarhus University Hospital

Aarhus, , Denmark

Site Status

Countries

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Denmark

References

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Wichmann TO, Kasch H, Dyrskog S, Hoy K, Moller BK, Krog J, Hoffmann HJ, Hviid CVB, Rasmussen MM. Glial fibrillary acidic protein is a robust biomarker in cerebrospinal fluid and peripheral blood after traumatic spinal cord injury: a prospective pilot study. Acta Neurochir (Wien). 2023 Jun;165(6):1417-1425. doi: 10.1007/s00701-023-05520-x. Epub 2023 Feb 15.

Reference Type DERIVED
PMID: 36790588 (View on PubMed)

Other Identifiers

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490-13-3632

Identifier Type: -

Identifier Source: org_study_id