A Study to Evaluate the Safety and Immunogenicity for Regimen Selection of Ad26.RSV.preF and/or RSV preF Protein Combinations Followed by Expanded Safety Evaluation in Adults Aged 60 Years and Older

NCT ID: NCT03502707

Last Updated: 2025-05-25

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

669 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-07-06

Study Completion Date

2022-05-16

Brief Summary

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The purpose of this study for:

Cohort 1 and Cohort 2: to assess the safety and reactogenicity of the intramuscular one- and two-dose regimens, with a booster at Month 12 (Cohort 1) and to select a regimen for Cohort 3.

Cohort 2 and part of Cohort 1: to assess respiratory syncytial virus (RSV) neutralizing antibody levels of the regimens containing RSV pre-fusion (preF) protein compared to the one-dose adenovirus serotype 26 respiratory syncytial virus pre-fusion (Ad26.RSV.preF) regimen.

Cohort 3: to assess the safety and reactogenicity of the selected regimen and a booster at Month 12 and/or Month 24.

Detailed Description

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Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Cohort (C)1 Group (G)1: Placebo for RSV preF Protein

Participants will receive intramuscular injection of placebo on Day 1, Day 57 and at Month 12.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

C1 G2: RSV preF Protein

Participants will receive intramuscular injection of 50 microgram (mcg) RSV preF protein on Day 1, Day 57 and at Month 12.

Group Type EXPERIMENTAL

RSV preF Protein 50 mcg

Intervention Type BIOLOGICAL

RSV preF will be administered as a solution for intramuscular injection at a dose of 50 mcg.

C1 G3: Placebo for Ad26.RSV.preF/RSV preF or RSV preF Protein

Participants will receive intramuscular injection of placebo on Day 1, Day 57 and at Month 12.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

C1 G4: Mixture of Ad26.RSV.preF/RSV preF Protein

Participants will receive intramuscular injection of a mixture of 5\*10\^10 viral particles (vp) of Ad26.RSV.preF/RSV preF 50 mcg protein on Day 1, Day 57 and at Month 12.

Group Type EXPERIMENTAL

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 50 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (50 mcg) will be administered as a solution for intramuscular injection.

C1 G5: RSV preF Protein

Participants will receive intramuscular injection of 150 mcg RSV preF protein on Day 1, Day 57 and at Month 12.

Group Type EXPERIMENTAL

RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

RSV preF will be administered as a solution for intramuscular injection at a dose of 150 mcg.

C1 G6: Mixture of Placebo for Ad26.RSV.preF/RSV preF Protein

Participants will receive intramuscular injection of placebo on Day 1, Day 57 and at Month 12.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

C1 G7: Mixture of Ad26.RSV.preF/RSV preF Protein

Participants will receive intramuscular injection of a mixture of 5\*10\^10 vp of Ad26.RSV.preF plus 150 mcg RSV preF protein on Day 1, Day 57 and at Month 12.

Group Type EXPERIMENTAL

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

C1 G8: Placebo for Ad26.RSV.preF/Placebo for RSV preF Protein

Participants will receive intramuscular injection of placebo on Day 1 and at Month 12 and in only 1 arm on Day 57.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

C1 G9: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Participants will receive intramuscular injection of a mixture of 1\*10\^11 vp of Ad26.RSV.preF plus 150 mcg RSV preF protein in 1 arm on Day 1 and 57 and at Month 12 and placebo in another arm on Day 1 and at Month 12.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

C1 G10: Ad26.RSV.preF, RSV preF Protein and Placebo

Participants will receive separate intramuscular injections of 1\*10\^11 vp of Ad26.RSV.preF in 1 Arm and 150 mcg RSV preF protein in another arm on Day 1 and at Month 12 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

RSV preF will be administered as a solution for intramuscular injection at a dose of 150 mcg.

Ad26.RSV.preF 1*10^11 vp

Intervention Type BIOLOGICAL

Ad26.RSV.preF will be administered as a solution for intramuscular injection at a dose of 1\*10\^11 vp.

C2 G11: Ad26.RSV.preF and Placebo

Participants will receive intramuscular injection of 1\*10\^11 vp of Ad26.RSV.preF in 1 arm and placebo in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Ad26.RSV.preF 1*10^11 vp

Intervention Type BIOLOGICAL

Ad26.RSV.preF will be administered as a solution for intramuscular injection at a dose of 1\*10\^11 vp.

C2 G12: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Participants will receive intramuscular injection of a mixture of 5\*10\^10 vp Ad26.RSV.preF plus 50 mcg RSV preF protein in 1 arm and placebo in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 50 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (50 mcg) will be administered as a solution for intramuscular injection.

C2 G13: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Participants will receive intramuscular injection of a mixture of 1\*10\^11 vp Ad26.RSV.preF plus 50 mcg RSV preF protein in 1 arm and placebo in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 50 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (50 mcg) will be administered as a solution for intramuscular injection.

C2 G14: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Participants will receive intramuscular injection of a mixture of 1\*10\^11 vp Ad26.RSV.preF plus 150 mcg RSV preF protein in 1 arm and placebo in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

C2 G15: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Participants will receive intramuscular injection of a mixture of 5\*10\^10 vp Ad26.RSV.preF plus 150 mcg RSV preF protein in 1 arm and placebo in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

C2 G16: Ad26.RSV.preF, RSV preF Protein and Placebo

Data from C1 G10 will be pooled with those of C2 G16. Participants will receive separate intramuscular injections of 1\*10\^11 vp of Ad26.RSV.preF in 1 Arm and 150 mcg RSV preF protein in another arm on Day 1 and placebo in 1 arm on Day 57.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

RSV preF will be administered as a solution for intramuscular injection at a dose of 150 mcg.

Ad26.RSV.preF 1*10^11 vp

Intervention Type BIOLOGICAL

Ad26.RSV.preF will be administered as a solution for intramuscular injection at a dose of 1\*10\^11 vp.

C2 G17: Mixture of Ad26.RSV.preF/RSV preF Protein and Placebo

Data from C1 G9 will be pooled with those of C2 G17. Participants will receive intramuscular injection of a mixture of 1\*10\^11 vp of Ad26.RSV.preF plus 150 mcg RSV preF protein in 1 arm on Day 1 and 57 and placebo in another arm on Day 1.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 150 mcg

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

C2 G18: Placebo for Ad26.RSV.preF/Placebo for RSV preF Protein

Participants will receive intramuscular injection of placebo in separate arms on Day 1 and in only 1 arm on Day 57.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

C3 G19: Selected Regimen (SR)

If a one-dose regimen is selected, participants in this group will receive SR on Day 1 and a booster (the SR) at Month 12 and month 24. The participants who are randomized to two-dose regimen will receive SR on Day 1 and Day 57, and a booster (the selected regimen) at Month 12.

Group Type EXPERIMENTAL

Selected Regimen

Intervention Type BIOLOGICAL

A regimen from Cohort 1 or Cohort 2 will be selected and administered as a solution for intramuscular injection at the selected dose.

C3 G20: SR + Placebo for SR

If a one-dose regimen is selected, participants in this group will receive SR on Day 1 and Month 24, and a placebo at Month 12. The participants who are randomized to two-dose regimen will receive selected regimen on Day 1 and Day 57, and a placebo at Month 12.

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Selected Regimen

Intervention Type BIOLOGICAL

A regimen from Cohort 1 or Cohort 2 will be selected and administered as a solution for intramuscular injection at the selected dose.

C3 G21: Placebo for SR

If a one-dose regimen is selected, participants in this group will receive placebo for SR on Day 1 and at Month 12 and Month 24. The participants who are randomized to two-dose regimen will receive placebo for SR on Day 1, Day 57, and Month 12.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Interventions

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Placebo

Placebo for Ad26.RSV.preF, RSV preF protein, Ad26.RSV.preF/RSV preF protein mixture and selected regimen will be administered as sterile saline for intramuscular injection.

Intervention Type BIOLOGICAL

RSV preF Protein 50 mcg

RSV preF will be administered as a solution for intramuscular injection at a dose of 50 mcg.

Intervention Type BIOLOGICAL

RSV preF Protein 150 mcg

RSV preF will be administered as a solution for intramuscular injection at a dose of 150 mcg.

Intervention Type BIOLOGICAL

Ad26.RSV.preF 1*10^11 vp

Ad26.RSV.preF will be administered as a solution for intramuscular injection at a dose of 1\*10\^11 vp.

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 50 mcg

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (50 mcg) will be administered as a solution for intramuscular injection.

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF 5*10^10 vp Plus RSV preF Protein 150 mcg

Mixture of Ad26.RSV.preF (5\*10\^10 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 50 mcg

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (50 mcg) will be administered as a solution for intramuscular injection.

Intervention Type BIOLOGICAL

Mixture of Ad26.RSV.preF 1*10^11 vp Plus RSV preF Protein 150 mcg

Mixture of Ad26.RSV.preF (1\*10\^11 vp) and RSV preF protein (150 mcg) will be administered as a solution for intramuscular injection.

Intervention Type BIOLOGICAL

Selected Regimen

A regimen from Cohort 1 or Cohort 2 will be selected and administered as a solution for intramuscular injection at the selected dose.

Intervention Type BIOLOGICAL

Other Intervention Names

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JNJ-64213175 JNJ-64213175 JNJ-64400141

Eligibility Criteria

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Inclusion Criteria

* Before randomization, a woman must be postmenopausal (postmenopausal state is defined as no menses for 12 months without an alternative medical cause) and not intending to conceive by any methods
* In the investigator's clinical judgment, participant must be either in good or stable health. Participants may have underlying illnesses such as hypertension, type 2 diabetes mellitus, hyperlipoproteinemia, or hypothyroidism, as long as their symptoms and signs are medically controlled. If they are on medication for a condition, the medication dose must have been stable for at least 12 weeks preceding vaccination and expected to remain stable for the duration of the study. Participants will be included on the basis of physical examination, medical history, vital signs, and 12-lead electrocardiogram (ECG) performed at screening
* For participants in Cohorts 1 and 2 only: Participant must be healthy on the basis of clinical laboratory tests performed at screening. If the results of the laboratory screening tests are outside the central laboratory normal reference ranges and additionally within the limits of toxicity Grade 2 according to the United States (US) Food and Drug Administration (FDA) toxicity, the participant may be included only if the investigator judges the abnormalities or deviations from normal to be not clinically significant and appropriate and reasonable for the population under study. This determination must be recorded in the participant's source documents and initialed by the investigator
* From the time of each vaccination through 3 months after each vaccination, participant agrees not to donate blood
* Participant must be willing to provide verifiable identification, have means to be contacted and to contact the investigator during the study

Exclusion Criteria

* Per serology testing in Cohorts 1 and 2 and per medical history in Cohort 3: Participant has chronic active hepatitis B or hepatitis C infection, documented by hepatitis B surface antigen and hepatitis C antibody, respectively
* Per serology testing in Cohorts 1 and 2 and per medical history in Cohort 3: Participant has human immunodeficiency virus (HIV) type 1 or type 2 infection
* Participant has had major psychiatric illness and/or drug or alcohol abuse which in the investigator's opinion would compromise the participant's safety and/or compliance with the study procedures
* Participant has a known allergy, or history of anaphylaxis or other serious adverse reactions to vaccines or vaccine components (including any of the constituents of the study vaccine)
* Participant has received respiratory syncytial virus (RSV) vaccine in a previous RSV vaccine study at any time prior to randomization
Minimum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Janssen Vaccines & Prevention B.V.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Janssen Vaccines & Prevention B.V. Clinical Trial

Role: STUDY_DIRECTOR

Janssen Vaccines & Prevention B.V.

Locations

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Optimal Research

Huntsville, Alabama, United States

Site Status

Optimal Research

San Diego, California, United States

Site Status

Optimal Research

Melbourne, Florida, United States

Site Status

Optimal Research

Peoria, Illinois, United States

Site Status

Optimal Research

Rockville, Maryland, United States

Site Status

Optimal Research

Austin, Texas, United States

Site Status

Countries

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United States

References

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Comeaux CA, Bart S, Bastian AR, Klyashtornyy V, De Paepe E, Omoruyi E, van der Fits L, van Heesbeen R, Heijnen E, Callendret B, Sadoff J. Safety, Immunogenicity, and Regimen Selection of Ad26.RSV.preF-Based Vaccine Combinations: A Randomized, Double-blind, Placebo-Controlled, Phase 1/2a Study. J Infect Dis. 2024 Jan 12;229(1):19-29. doi: 10.1093/infdis/jiad220.

Reference Type DERIVED
PMID: 37433021 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Other Identifiers

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VAC18193RSV1004

Identifier Type: OTHER

Identifier Source: secondary_id

CR108456

Identifier Type: -

Identifier Source: org_study_id

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