Heritability of Polycystic Ovary Syndrome: Role of Antimullerian Hormone, Steroids and Leptin

NCT ID: NCT03483792

Last Updated: 2025-12-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

58 participants

Study Classification

OBSERVATIONAL

Study Start Date

2018-04-20

Study Completion Date

2022-06-02

Brief Summary

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Polycystic ovary syndrome (PCOS) is the most common cause of ovulation disorders and affects 10 to 15% of women. Despite its frequency, its physiopathology remains unknown.

In women, Anti-Müllerian hormone (AMH) is secreted by granulosa cells located in the ovaries within the follicles. Compared to control women, serum AMH level is higher in PCOS women and could play a role in its pathophysiology. The severity of the PCOS phenotype is correlated with the production of AMH.

It is currently described in the literature that daughters of women with PCOS have a 50% risk of developing PCOS, but no genetic cause of transmission is known. In mice (article in press), pregnant females injected with AMH give birth to offspring with PCOS symptoms. The AMH could thus also play a role in the heritability of PCOS in women. Our team demonstrated that AMH, in its active cleaved form, had a direct central action on the hypothalamus by increasing the pulsatility of GnRH, inducing LH hypersecretion. The hypothesis is that AMH remains higher in pregnant women with PCOS and may affect the fetus by altering fetal and maternal hypothalamic secretions or by modifying placental steroid production.

Leptin has a role in reproduction, through its receptors located at the central (hypothalamus) and peripheral (granulosa cells) levels. In excessively high serum concentration, as observed in obesity, it would lead to a dysregulation of GnRH secretion, an alteration of ovarian steroidogenesis and a dysregulation of folliculogenesis.

Will be compare leptin levels in first trimester patients with and without PCOS to look for possible correlations between AMH and leptin and eliminate possible bias.

Detailed Description

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Conditions

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Polycystic Ovary Syndrome

Keywords

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PCOS AMH Pregnancy Heritability LH

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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PCOS group

Plasma dosage

Intervention Type BIOLOGICAL

4 x 7 ml of blood punction at each control visit of the three trimesters of pregnancy

placental biopsy

Intervention Type GENETIC

Immediately following delivery (\<12h postpartum), placental biopsies (Collection of 4 placental fragments)

Control group

Plasma dosage

Intervention Type BIOLOGICAL

4 x 7 ml of blood punction at each control visit of the three trimesters of pregnancy

placental biopsy

Intervention Type GENETIC

Immediately following delivery (\<12h postpartum), placental biopsies (Collection of 4 placental fragments)

Interventions

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Plasma dosage

4 x 7 ml of blood punction at each control visit of the three trimesters of pregnancy

Intervention Type BIOLOGICAL

placental biopsy

Immediately following delivery (\<12h postpartum), placental biopsies (Collection of 4 placental fragments)

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Having a pre-conceptional infertility assessment in the gynecology-Endocrinology department of University Hospital of Lille
* in the first trimester of mono fetal pregnancy (between 5 and 10 weeks of gestation), obtained spontaneously, after induction of ovulation or Assisted Reproductive Techniques (ART)
* Pregnancy followed at University Hospital of Lille
* PCOS group: defined according to modified Rotterdam criteria (2003 and 2011)
* At least 2 of the following 3 criterion:

* Cycle disorder
* Clinical and / or biological hyperandrogenism
* Ovarian volume \> 10cm³ and/or more than 19 follicles from 2 to 9 mm per ovary
* After exclusion of other causes of cycle disorder or hyperandrogenism
* Control group: patient with severe male and / or tubal infertility, no cycling disorder, normal ovarian reserve (FSH\<10 IU / L, E2\<50 pg / ml, AMH\>7 and \<35 pmol / L and Follicles count between \>5 and \<20 per ovary at day 3 of the cycle).

In the group of female controls, the fertility problem is not related to a female pathology of the hypothalamic-pituitary-ovarian axis (tubal or male infertility). They are women without ovarian personal pathology. The problem of fertility being of other origin.

Exclusion Criteria

* Multiple pregnancy
* Pregnancy after egg donation
* Long-term drug therapy (excluding routine pregnancy supplementation)
* Previous Diabetes
* Bariatric surgery
* Patients with ovulatory infertility of central or idiopathic origin
* Patients already included in another protocol
Minimum Eligible Age

18 Years

Maximum Eligible Age

43 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Lille

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Sophie Catteau-Jonard, MD,PhD

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Lille

Locations

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Hôpital Jeanne de Flandres, CHU

Lille, , France

Site Status

Countries

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France

References

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Peigne M, Simon V, Pigny P, Mimouni NEH, Martin C, Dewailly D, Catteau-Jonard S, Giacobini P. Changes in circulating forms of anti-Muullerian hormone and androgens in women with and without PCOS: a systematic longitudinal study throughout pregnancy. Hum Reprod. 2023 May 2;38(5):938-950. doi: 10.1093/humrep/dead050.

Reference Type RESULT
PMID: 36921289 (View on PubMed)

Other Identifiers

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2017-A02628-45

Identifier Type: OTHER

Identifier Source: secondary_id

2017_11

Identifier Type: -

Identifier Source: org_study_id