Rescuing the Microbiome Effectively With Different Doses of B. Infantis in Infants

NCT ID: NCT03476447

Last Updated: 2019-04-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-04-06

Study Completion Date

2019-04-03

Brief Summary

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The REMEDI Study is a single-center, prospective, double-blind, randomized, placebo-controlled trial of multiple doses of B. infantis EVC001 supplementation in healthy term breastfed infants.

Detailed Description

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Mother-infant dyads will be enrolled when the infant is 2-4 months of age. A 1-week lead-in period will be utilized to collect baseline microbiome samples and for the completion of daily and weekly feeding and health logs. Subjects that meet eligibility criteria on Day 7 will be randomized into 1 of 4 treatment arms. Supplementation will begin on Day 8 and will continue for a total of 28 consecutive days. Subjects will be followed for an additional 4 weeks after the cessation of the supplement. The total duration of the study will be approximately 9 weeks. Infant stool and urine samples, as well as maternal breast milk samples, will be collected on multiple occasions during the study. Mothers will be asked to complete questionnaires and health logs during the course of the study as well.

Conditions

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Gut Microbiome

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Subjects will be enrolled and randomized 1:1:1:1 to the following treatment arms (daily dose of B. infantis EVC001): high dose, medium dose, low dose, and placebo.
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors
Participants, investigators and data evaluators will be blinded to the treatment assignments.

Study Groups

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High dose B. infantis EVC001

10 participants will receive powdered B. infantis EVC001 at a high dose per daily oral feeding

Group Type ACTIVE_COMPARATOR

B. infantis EVC001

Intervention Type OTHER

A once-daily oral feeding of B. infantis EVC001 will be mixed with breast milk and provided to infants for 28 consecutive days.

Medium dose B. infantis EVC001

10 participants will receive powdered B. infantis EVC001 at a medium dose per daily oral feeding

Group Type ACTIVE_COMPARATOR

B. infantis EVC001

Intervention Type OTHER

A once-daily oral feeding of B. infantis EVC001 will be mixed with breast milk and provided to infants for 28 consecutive days.

Low dose B. infantis EVC001

10 participants will receive powdered B. infantis EVC001 at a low dose per daily oral feeding

Group Type ACTIVE_COMPARATOR

B. infantis EVC001

Intervention Type OTHER

A once-daily oral feeding of B. infantis EVC001 will be mixed with breast milk and provided to infants for 28 consecutive days.

Lactose Placebo

10 participants will receive powdered lactose per daily oral feeding

Group Type PLACEBO_COMPARATOR

Lactose

Intervention Type OTHER

A once-daily oral feeding of a lactose placebo will be mixed with breast milk and provided to infants for 28 consecutive days.

Interventions

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B. infantis EVC001

A once-daily oral feeding of B. infantis EVC001 will be mixed with breast milk and provided to infants for 28 consecutive days.

Intervention Type OTHER

Lactose

A once-daily oral feeding of a lactose placebo will be mixed with breast milk and provided to infants for 28 consecutive days.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Age of subjects: Infants: 60 to 125 days old, Mothers: 21 years old and greater
* Mother-infant dyads who live within a 40-mile radius from the clinical site
* Infants predominately breastfed with maternal intent to continue exclusive breastfeeding for at least 9 additional weeks
* Term infants born \>37 weeks gestation
* Healthy infants without medical complications
* Generally healthy women
* Mothers who are willing to refrain from feeding their infants infant formula, solid foods, and probiotic or iron supplements (confounding variables of the intestinal microbiome) before the end of the study

Exclusion Criteria

* Mothers diagnosed with any metabolic, endocrine, liver, or kidney disease, any autoimmune disease, cirrhosis, hepatitis C, HIV, AIDs, cancer, obesity (pre-pregnancy BMI \>34.9 kg/m2), Crohn's disease, heart disease, type 1 or type 2 diabetes (type 1 diabetes is acceptable if mother's blood glucose has been well-regulated)
* Infants born in a multiple birth
* Infants born with medical complications such as: respiratory distress syndrome or birth defects
* Infants with any GI tract abnormalities
* Infants who have taken antibiotics within 4 weeks of enrollment or during the Lead-in Period
* Infants who have taken iron supplements within 4 weeks of enrollment or during the Lead-in Period
* Infants who have consumed any infant formula within 4 weeks of enrollment or more than 10 times between birth and enrollment or during the Lead-in Period
* Infants whose parents plan to feed them any infant formula any time throughout the duration of the study
* Infants who have consumed any probiotics containing B. infantis since birth
* Infants who have consumed any Bifidobacterium-containing probiotics within 4 weeks of enrollment or during the Lead-in Period
* Infants whose parents plan to administer probiotics to them any time throughout the duration of the study (not including the study supplement)
* Infants who have consumed any solid foods or beverages between birth and enrollment or during the Lead-in Period (liquid medicines, supplements, and sugar water are ok)
* Infants whose parents plan to feed them solid foods or beverages any time throughout the duration of the study
* Infants whose mothers have changed their minds about their plans to exclusively breastfeed for at least 8 additional weeks by Day 7
* For infants born vaginally, maternal intake of probiotics containing B. infantis during the last trimester of pregnancy
* Mother-infant dyads who live in more than one location
* Infants who have been diagnosed with any medical or nutritional condition that would require iron supplementation
* Mothers who smoked cigarettes during pregnancy, currently smoke, or plan to initiate smoking during the study duration
* Anyone the investigator feels isn't an applicable subject
Minimum Eligible Age

2 Months

Maximum Eligible Age

4 Months

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of California, Davis

OTHER

Sponsor Role collaborator

Evolve BioSystems, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jennifer Smilowitz, PhD

Role: PRINCIPAL_INVESTIGATOR

University of California, Davis

Locations

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University of California, Davis

Davis, California, United States

Site Status

Countries

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United States

Other Identifiers

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1166403

Identifier Type: -

Identifier Source: org_study_id

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