Trial Outcomes & Findings for A Study of the Safety, Engraftment, and Action of NB01 in Adults With Moderate Acne (NCT NCT03450369)

NCT ID: NCT03450369

Last Updated: 2020-08-03

Results Overview

Engraftment is defined as the percentage of bacteria recovered from hair follicles that are health-associated (NB01) as measured with proprietary quantitative polymerase chain assays. Successful follicular engraftment, defined as both deoR/PanBac \>40% and Cas5/PanBac \>40% in the Day 2 (24H) ) Biore Strip samples. Cas5/PanBac and deoR/PanBac are the percentages of bacteria in a sample containing the CRISPR associated protein Cas5 and a repressor of porphyrin production deoR, respectively. Pan-Bacterial (PanBac) is a loci found in nearly all bacteria found on the face and thus serves as the denominator to calculate the percentage of bacteria with any loci.

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

10 participants

Primary outcome timeframe

2 Months

Results posted on

2020-08-03

Participant Flow

Participant milestones

Participant milestones
Measure
Low Dose
10\^4 CFU/cm\^2 NB01 colony forming unit (CFU)
High Dose
10\^5 CFU/cm\^2 NB01 colony forming unit (CFU)
Overall Study
STARTED
5
5
Overall Study
COMPLETED
5
5
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

A Study of the Safety, Engraftment, and Action of NB01 in Adults With Moderate Acne

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Total
n=10 Participants
Total of all reporting groups
Age, Continuous
22.6 years
STANDARD_DEVIATION 4.77 • n=5 Participants
26.6 years
STANDARD_DEVIATION 3.78 • n=7 Participants
24.6 years
STANDARD_DEVIATION 4.58 • n=5 Participants
Sex: Female, Male
Female
5 Participants
n=5 Participants
4 Participants
n=7 Participants
9 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
2 Participants
n=5 Participants
0 Participants
n=7 Participants
2 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
3 Participants
n=5 Participants
5 Participants
n=7 Participants
8 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
2 Participants
n=7 Participants
2 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
White
4 Participants
n=5 Participants
2 Participants
n=7 Participants
6 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
5 participants
n=5 Participants
5 participants
n=7 Participants
10 participants
n=5 Participants
Reproductive Status
Able to bear children
5 Participants
n=5 Participants
4 Participants
n=7 Participants
9 Participants
n=5 Participants
Reproductive Status
Total abstinence (male)
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Method of Contraception (Female)
Total abstinence
2 Participants
n=5 Participants
0 Participants
n=7 Participants
2 Participants
n=5 Participants
Method of Contraception (Female)
Barrier method with spermicide
3 Participants
n=5 Participants
0 Participants
n=7 Participants
3 Participants
n=5 Participants
Method of Contraception (Female)
Oral, injected or implanted combined hormonal meth
0 Participants
n=5 Participants
4 Participants
n=7 Participants
4 Participants
n=5 Participants
Method of Contraception (Female)
male subject: not recorded
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Investigator's Global Assessment (IGA) Score - Face
3 units on a scale
STANDARD_DEVIATION 0 • n=5 Participants
3 units on a scale
STANDARD_DEVIATION 0 • n=7 Participants
3 units on a scale
STANDARD_DEVIATION 0 • n=5 Participants

PRIMARY outcome

Timeframe: 2 Months

Engraftment is defined as the percentage of bacteria recovered from hair follicles that are health-associated (NB01) as measured with proprietary quantitative polymerase chain assays. Successful follicular engraftment, defined as both deoR/PanBac \>40% and Cas5/PanBac \>40% in the Day 2 (24H) ) Biore Strip samples. Cas5/PanBac and deoR/PanBac are the percentages of bacteria in a sample containing the CRISPR associated protein Cas5 and a repressor of porphyrin production deoR, respectively. Pan-Bacterial (PanBac) is a loci found in nearly all bacteria found on the face and thus serves as the denominator to calculate the percentage of bacteria with any loci.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Successful Engraftment of Probiotic Following a Single Application.
2 Participants
1 Participants

PRIMARY outcome

Timeframe: From Baseline to 48 hours after application

To determine longevity of the live biotherapeutic after a single application, swab samples were collected at Baseline, 6, 24 and 48 hours after application, and genotyped as above. For each participant the absolute change, from Baseline, in the percentage of the two loci present in the live biotherapeutic (deoR and Cas5) were calculated. A significant positive change is indicative of the continued presence of the live biotherapeutic.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 1 (deoR), 6 hours
-0.4 % of total bacteria w/ genotype
Standard Deviation 36.3
51.2 % of total bacteria w/ genotype
Standard Deviation 28.6
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 1 (deoR), 24 hours
-4.2 % of total bacteria w/ genotype
Standard Deviation 25.7
5.2 % of total bacteria w/ genotype
Standard Deviation 7.6
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 1 (deoR), 48 hours
13.8 % of total bacteria w/ genotype
Standard Deviation 33.5
2.8 % of total bacteria w/ genotype
Standard Deviation 10.9
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 2 (Cas 5), 6 hours
12.8 % of total bacteria w/ genotype
Standard Deviation 27.9
48.4 % of total bacteria w/ genotype
Standard Deviation 21.7
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 2 (Cas 5), 24 hours
3.4 % of total bacteria w/ genotype
Standard Deviation 15.6
4.4 % of total bacteria w/ genotype
Standard Deviation 8.8
Dose Schedule Determination Based on Time to Peak deoR and Cas5
Genotype 2 (Cas 5), 48 hours
3.2 % of total bacteria w/ genotype
Standard Deviation 12.2
1.8 % of total bacteria w/ genotype
Standard Deviation 12.1

PRIMARY outcome

Timeframe: 28 Days

Number of participants with a changed Investigator Global Assessment (IGA) score after single application of NB01; positive number if grade is reduced (e.g. from 3 to 2) or negative number is IGA grade increased. The Investigator Global Assessment 5-Point Score is a standard FDA acne assessment tool with scoring as follows: Grade 0-Clear (Clear skin with no inflammatory or non-inflammatory lesions. The category of clear should represent true absence of disease) Grade1-Almost Clear (A few scattered comedones and no more than one small papule) Grade 2-Mild (Some comedones, some papules and pustules; no nodules) Grade 3-Moderate (Many comedones, papules and pustules; one nodule may be present) Grade 4-Severe (Covered with comedones, numerous papules and pustules and no more than a few nodular lesions)

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Global Assessment (IGA) Score
Participants with changed IGA @ 6 hours
0 Participants
0 Participants
Number of Participants With Change in Investigator Global Assessment (IGA) Score
Participants with changed IGA @ 1 day
0 Participants
0 Participants
Number of Participants With Change in Investigator Global Assessment (IGA) Score
Participants with changed IGA @ 2 days
0 Participants
0 Participants
Number of Participants With Change in Investigator Global Assessment (IGA) Score
Participants with changed IGA @ 6 days
0 Participants
0 Participants
Number of Participants With Change in Investigator Global Assessment (IGA) Score
Participants with changed IGA @ 28 days
2 Participants
0 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Absolute Lesion Count: All Lesions for the Whole Face; with higher numbers of lesion representing worsening or more severe acne

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Screening
49.4 count of lesions
Standard Deviation 21.08
38.8 count of lesions
Standard Deviation 16.60
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 1 (0 hours)
22.6 count of lesions
Standard Deviation 13.26
42.6 count of lesions
Standard Deviation 3.8
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 1 (6 hours)
21.0 count of lesions
Standard Deviation 9.22
39.8 count of lesions
Standard Deviation 25.41
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 2
31.6 count of lesions
Standard Deviation 3.51
38.2 count of lesions
Standard Deviation 25.29
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 3
32.2 count of lesions
Standard Deviation 6.18
35.4 count of lesions
Standard Deviation 24.17
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 7
25.6 count of lesions
Standard Deviation 4.72
34.2 count of lesions
Standard Deviation 19.11
Safety Profile and Tolerability of a Singly Applied NB01 Using Acne Lesion Counts.
Day 28
25.8 count of lesions
Standard Deviation 14.31
32.6 count of lesions
Standard Deviation 59.22

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Skin Peeling. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Screening, scale = 1 (slight)
2 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 28, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Screening, scale = 0 (none)
3 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 1 (0 hours), scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 1 (0 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 1 (6 hours), scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 1 (6 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 3, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Peeling
Day 7, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Irritant/Allergic Contact Dermatitis. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Screening, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 1 (6 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 3, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 1 (0 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Irritant/Allergic Contact Dermatitis
Day 28, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Hyperpigmentation. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 1 (0 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Screening, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 1 (6 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 3, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Hyperpigmentation
Day 28, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Ocular Irritation. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 28, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Screening, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 1 (0 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 1 (6 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Ocular Irritation
Day 3, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Conjunctival Injection. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 1 (6 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Screening, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 1 (0 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 3, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Conjunctival Injection
Day 28, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Mucosal Toxicity. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Screening, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 1 (0 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 1 (6 hours), scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 2, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 3, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Mucosal Toxicity
Day 28, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Skin Erythema. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Screening, scale = 0 (none)
2 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Screening, scale = 1 (slight)
3 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (0 hours), scale = 0 (none)
3 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (0 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (0 hours), scale = 2 (moderate)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (6 hours), scale = 0 (none)
3 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (6 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 1 (6 hours), scale = 2 (moderate)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 2, scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 2, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 3, scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 3, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 7, scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 7, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Erythema.
Day 28, scale = 0 (none)
5 Participants
5 Participants

PRIMARY outcome

Timeframe: From Screening to 28 days after application, typically 2 months

Population: All participants who received at least one dose of treatment.

Safety profile and tolerability of a singly applied NB01 using the Investigator Assessment of Tolerability Scoring (0 = None; 1 = Slight; 2 = Moderate; 3 = Intense) for Skin Dryness. Reference: ClinicalTrials.gov: Investigator Assessment Tolerability Scoring: A Study to Evaluate Tolerability of Two Topical Drug Products in the Treatment of Acne.

Outcome measures

Outcome measures
Measure
Low Dose
n=5 Participants
10\^4 CFU/cm\^2 NB01
High Dose
n=5 Participants
10\^5 CFU/cm\^2 NB01
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Screening, scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Screening, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 1 (0 hours), scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 3, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 7, scale = 0 (none)
5 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 28, scale = 0 (none)
3 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 28, scale = 1 (slight)
2 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 1 (0 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 1 (6 hours), scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 1 (6 hours), scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 2, scale = 0 (none)
4 Participants
5 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 2, scale = 1 (slight)
1 Participants
0 Participants
Number of Participants With Change in Investigator Assessment of Tolerability Score: Skin Dryness.
Day 3, scale = 0 (none)
4 Participants
5 Participants

Adverse Events

Low Dose

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

High Dose

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Low Dose
n=5 participants at risk
10\^4 CFU/cm\^2 NB01
High Dose
n=5 participants at risk
10\^5 CFU/cm\^2 NB01
General disorders
Application site dermatitis
20.0%
1/5 • Number of events 1 • From Screening to 28 days after application, typically 2 months
0.00%
0/5 • From Screening to 28 days after application, typically 2 months
Skin and subcutaneous tissue disorders
Dry skin
0.00%
0/5 • From Screening to 28 days after application, typically 2 months
20.0%
1/5 • Number of events 1 • From Screening to 28 days after application, typically 2 months

Additional Information

Emma Taylor, MD CEO

Naked Biome

Phone: 626-260-1230

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place