A Study to Evaluate the Safety, PK, PD, Immunogenicity of N-Rephasin® SAL200 in Healthy Male Volunteers

NCT ID: NCT03446053

Last Updated: 2021-11-03

Study Results

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

33 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-02-07

Study Completion Date

2019-02-07

Brief Summary

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To evaluate the safety, pharmacokinetics, pharmacodynamics and immunogenicity of N-Rephasin® SAL200 following single and multiple ascending doses in healthy male volunteers after continuous intravenous infusion over 60 minutes.

Detailed Description

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* Forty subjects will be randomly assigned to receive either N-Rephasin® SAL200 injection or a placebo administered by a 60-min intravenous infusion. Within each group, 8 subjects (6 active and 2 placebo) will receive a single dose of 6 mg/kg, followed by multiple ascending dose of 3, 6, 9, and 12 mg/kg/day. The clinical trial will be performed in sequence from the lowest level, and the progress of the next dose level will be determined based on the safety results in the previous dose level.
* Examinations by interview, physical examinations, and screening tests such as clinical laboratory tests and allergenicity will be conducted in volunteers within 4 weeks (-28\~-2d) from the previous day ot this clinical trial (-1d) to select subjects who are judged eligible for the study.
* Eligible subjects will be called in the afternoon (-1d) to examine the allergenicity in the clinical center of Seoul National Unviersity Hospital.
* Allergen-free subjects will be hospitalized on -1d and assigned with each subject number. All subjects are advised to fast from 10:00 p.m. overnight to the next morning, except drinking water. In the morning (09:00 a.m.) on 1d, subjects in each group will be given N-Rephasin® SAL200 injection or a placebo administered by a 60-min intravenous infusion, After that, the clinical trial will be conducted according to the designated schedule.
* All subjects receiving at least one dose will undergo a post-study visit test after a certain period of time. Immunogenicity testing will be performed until approximately 50 days after treatment.

Conditions

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Healthy Volunteers Anti-Bacterial Agents Methicillin-Resistant Staphylococcus Aureus Methicillin-Sensitive Staphylococcus Aureus Infection

Keywords

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Methicillin-Resistant Staphylococcus Aureus Methicillin-Sensitive Staphylococcus Aureus N-Rephasin SAL200

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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N-Rephasin® SAL200

Forty subjects will be randomly assigned to receive either N-Rephasin® SAL200 injection or a placebo administered by a 60-min intravenous infusion. Within each group, 8 subjects (6 active and 2 placebo) will receive a single dose of 6 mg/kg, followed by multiple ascending dose of 3, 6, 9, and 12 mg/kg/day.

Group Type EXPERIMENTAL

N-Rephasin® SAL200

Intervention Type BIOLOGICAL

continuous intravenous infusion over 60 minutes

INT200-Placebo

Saline

Group Type PLACEBO_COMPARATOR

INT200-Placebo

Intervention Type OTHER

Formulation buffer except active ingredient for continuous intravenous infusion over 60 minutes

Interventions

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N-Rephasin® SAL200

continuous intravenous infusion over 60 minutes

Intervention Type BIOLOGICAL

INT200-Placebo

Formulation buffer except active ingredient for continuous intravenous infusion over 60 minutes

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Healthy male subjects aged between 20 and 45 years at screening
* Those whose body weight is between 50kg and 90kg, and BMI is between 18.0 and 27.0
* Subjects who have fully understood this clinical trial via detailed explanation, are willing to voluntarily participate in this study, and agree to give written informed consent and to follow all of trial-related rules.

Exclusion Criteria

* Those who have clinically significant liver, kidney, nervous system, endocrine system, respiratory system, hemato-oncology, cardiovascular system, mental diseases or past history.
* Those who have been diagnosed or suspected infectious disease within 30 days prior to the first dose of study medication
* Those who have history of hypersensitivity to drugs containing N-Rephasin® SAL200 or other drugs (aspirin and antibiotics)
* Those who have taken other drugs containing N-Rephasin® SAL200.
* Those who are antibody-positive to N-Rephasin® SAL200
* Those who have SBP \<90mmHg or DBP \<50mmHg (otherwise SBP \> 150mmHg or DBP \> 100mmHg) in vital signs, when measured after a 3-minute rest in sitting position.
* Those who have medical history of drug abuse or positive to urine drug screening
* Has taken any prescription drugs or herbal medicines within 14 days prior to first dose of study medication; otherwise, has taken over-the-counter drugs or vitamins within 7 days prior to the first dose of study medication (However, if other conditions are appropriate upon judgment of the investigator, the subject may participate in this study.)
* Those who has taken other study medications within 3 months prior to the study medication
* Those who have donated whole blood within 2 months prior to the first dose of study medication or apheresis within 1 month, or received blood transfusion within 1 months prior to the first dose of study medication
* Those who smoke cigarettes or are found to be nicotine metabolite-positive in urinalysis
* Those who cannot continuously abstain from drinking alcohol (exceeding 21 units/week, 1 unit = 10g of pure alcohol) or smoking cigarettes during hospitalization
* Those who are judged ineligible for the clinical study by the investigator due to other reasons, including the results of clinical laboratory tests
* Those who do not agree to use medically accepted contraceptive measures for 60 days after the first dose of study medication, or those who are unwilling to report the partner's pregnancy until 90 days after the first dose of study medication
Minimum Eligible Age

20 Years

Maximum Eligible Age

45 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Intron Biotechnology, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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In Jin Jang, M.D., Ph. D.

Role: PRINCIPAL_INVESTIGATOR

Seoul National University Hospital

Locations

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Seoul National University Hospital

Seoul, , South Korea

Site Status

Countries

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South Korea

References

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Etienne J, Fleurette J, Ninet JF, Favet P, Gruer LD. Staphylococcal endocarditis after dental extraction. Lancet. 1986 Aug 30;2(8505):511-2. doi: 10.1016/s0140-6736(86)90377-6. No abstract available.

Reference Type BACKGROUND
PMID: 2875256 (View on PubMed)

Kundsin RB. Documentation of airborne infection during surgery. Ann N Y Acad Sci. 1980;353:255-61. doi: 10.1111/j.1749-6632.1980.tb18928.x. No abstract available.

Reference Type BACKGROUND
PMID: 6939390 (View on PubMed)

van Hal SJ, Jensen SO, Vaska VL, Espedido BA, Paterson DL, Gosbell IB. Predictors of mortality in Staphylococcus aureus Bacteremia. Clin Microbiol Rev. 2012 Apr;25(2):362-86. doi: 10.1128/CMR.05022-11.

Reference Type BACKGROUND
PMID: 22491776 (View on PubMed)

Wire MB, Jun SY, Jang IJ, Lee SH, Hwang JG, Huang DB. A Phase 1 Study To Evaluate Safety and Pharmacokinetics following Administration of Single and Multiple Doses of the Antistaphylococcal Lysin LSVT-1701 in Healthy Adult Subjects. Antimicrob Agents Chemother. 2022 Mar 15;66(3):e0184221. doi: 10.1128/AAC.01842-21. Epub 2022 Jan 10.

Reference Type DERIVED
PMID: 35007129 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Related Links

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https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001432619

Seung-Ho Han et al., Monitoring of Methicillin-Resistant Staphylococcus aureus in Nasal Swabs Obtained fron Dental Clinic Healthcare Providers and Medical Environments Nurses. Int J Oral Biology. 2010;35:7-12

https://www.semanticscholar.org/paper/Analysis-of-Economic-Outcomes-of-Staphylococcus-Park-Chae/f848ff1bc9cf6e346dbbc24bd4b4bf669dd70b43

Eun Ja Park et al., Analysis of Economic Outcome of Methicillin-Resistant Staphylococcus aureus(MRSA) Bacteremia Using Retrospective Case-Control Study, Kor. J. Clin. Pharm 2007;17:59-64

https://ir.ymlib.yonsei.ac.kr/handle/22282913/147505

Hyuk Min Lee, Dong Eun Yong, Kyungwon Lee., Antimicrobial Resistance of Clinically Important Bacteria Isolated from 12 Hospitals in Korea in 2004 . Korean Journal of Clinical Microbiology 2005;8(1):66-73

https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-38-2-168

Frency J, Brun Y, Bes M, Meugnier H, Grimont F, Grimon PAD, Newi C, Fleurette J. Staphylococcus lugdunensis sp. and Staphylococcus schleiferi sp. novel two species from human clinical specimens. Int Syst Bacteriol. 1998;38:168-172

Other Identifiers

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ITB-101_1b

Identifier Type: -

Identifier Source: org_study_id