Neoantigen-based Personalized Vaccine Combined With Immune Checkpoint Blockade Therapy in Patients With Newly Diagnosed, Unmethylated Glioblastoma

NCT ID: NCT03422094

Last Updated: 2021-10-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE1

Total Enrollment

3 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-10-31

Study Completion Date

2020-12-31

Brief Summary

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This is a single institution, open-label, multi-arm, pilot study assessing the safety, feasibility, and immunogenicity of a personalized neoantigen-based vaccine plus poly-ICLC (NeoVax) combined with immune checkpoint inhibitors in subjects with newly diagnosed, unmethylated glioblastoma.

Detailed Description

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Conditions

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Glioblastoma

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Cohort A: NeoVax+Nivolumab (start at time of progression)

* NeoVax given on Days 1, 8, 15, and 22 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)
* Nivolumab 480 mg i.v. given on Day 1 of each cycle beginning at time of progression

Group Type EXPERIMENTAL

NeoVax

Intervention Type BIOLOGICAL

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Nivolumab

Intervention Type BIOLOGICAL

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Research blood draw

Intervention Type PROCEDURE

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Leukapheresis for research

Intervention Type PROCEDURE

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Cohort B: NeoVax+Nivolumab (start with Cycle 2)

* NeoVax given on Days 1, 8, 15, and 22 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)
* Nivolumab 480 mg i.v. given on Day 1 of each cycle beginning with Cycle 2 (start of boosting phase)

Group Type EXPERIMENTAL

NeoVax

Intervention Type BIOLOGICAL

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Nivolumab

Intervention Type BIOLOGICAL

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Research blood draw

Intervention Type PROCEDURE

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Leukapheresis for research

Intervention Type PROCEDURE

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Cohort C: NeoVax + Nivolumab (start with Cycle 1)

* NeoVax given on Days 1, 8, 15, and 22 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)
* Nivolumab 480 mg i.v. given on Day 1 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)

Group Type EXPERIMENTAL

NeoVax

Intervention Type BIOLOGICAL

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Nivolumab

Intervention Type BIOLOGICAL

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Research blood draw

Intervention Type PROCEDURE

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Leukapheresis for research

Intervention Type PROCEDURE

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Cohort D: NeoVax+Ipilimumab+Nivolumab (start with Cycle 3)

* NeoVax given on Days 1, 8, 15, and 22 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)
* Ipilimumab 1 mg/kg i.v. given on Days 1 and 22 of Cycle 1 (priming phase)
* Nivolumab 480 mg i.v. given on Day 1 of Cycle 3 and then on Day 1 of each subsequent cycle

Group Type EXPERIMENTAL

NeoVax

Intervention Type BIOLOGICAL

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Nivolumab

Intervention Type BIOLOGICAL

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Ipilimumab

Intervention Type BIOLOGICAL

Ipilimumab is a recombinant, human monoclonal antibody that binds to the cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4)

Research blood draw

Intervention Type PROCEDURE

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Leukapheresis for research

Intervention Type PROCEDURE

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Cohort E: NeoVax+Ipilimumab+Nivolumab (day 1&15 each cycle)

* NeoVax given on Days 1, 8, 15, and 22 of Cycle 1 (priming phase), and then on Day 1 of each subsequent cycle (boosting phase)
* Ipilimumab 1 mg/kg i.v. given every 6 weeks beginning on Day 1 of Cycle 1 (C1D1, C2D15, C4D1, C5D15, C7D1, C8D15 …)
* Nivolumab 3 mg/kg i.v. given on Days 1 and 15 of each cycle (q2w) beginning on Day 1 of Cycle 1

Group Type EXPERIMENTAL

NeoVax

Intervention Type BIOLOGICAL

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Nivolumab

Intervention Type BIOLOGICAL

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Ipilimumab

Intervention Type BIOLOGICAL

Ipilimumab is a recombinant, human monoclonal antibody that binds to the cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4)

Research blood draw

Intervention Type PROCEDURE

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Leukapheresis for research

Intervention Type PROCEDURE

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Interventions

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NeoVax

At each vaccination time point, patients will receive up to 20 synthetic long peptides co-administered with 1.5 mg of poly-ICLC divided into a maximum of four injections (pools). Each pool (of vaccine + poly IC:LC) will be administered to one of the four limbs (right axilla, left axilla, right inguina, left inguina) by subcutaneous injection.

Intervention Type BIOLOGICAL

Nivolumab

Nivolumab is a programmed death receptor-1 (PD-1) blocking antibody

Intervention Type BIOLOGICAL

Ipilimumab

Ipilimumab is a recombinant, human monoclonal antibody that binds to the cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4)

Intervention Type BIOLOGICAL

Research blood draw

-Baseline, cycle 2 day 1, cycle 4 day 1, and time of progression or discontinuation of treatment

Intervention Type PROCEDURE

Leukapheresis for research

-Baseline, cycle 4 day 1, and time of progression or discontinuation of treatment

Intervention Type PROCEDURE

Other Intervention Names

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Synthetic long peptides plus poly-ICLC Opdivo Yervoy

Eligibility Criteria

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Inclusion Criteria

* Newly diagnosed histologically confirmed unmethylated glioblastoma multiforme (WHO grade IV). Patients with secondary glioblastoma, in particular those who are IDH1 or IDH2 mutant, will not be excluded. Unmethylated MGMT must be confirmed by a PCR-based assay.
* Patients who had craniotomy with biopsy, subtotal resection, total gross resection, or re-resection will be permitted.
* Consented to genome sequencing and dbGaP-based data sharing and has provided or will provide germline (PBMC) and tumor DNA/RNA samples of adequate quality for sequencing. (Acquisition of specimens for sequencing and the sequencing itself may be done as part of routine care or another research project.)
* At least 18 years of age.
* Karnofsky performance status ≥ 60%
* Normal bone marrow and organ function as defined below:

* Absolute neutrophil count ≥ 1,500/mcL
* Platelets ≥ 100,000/mcL
* Total bilirubin ≤ 1.5 x institutional upper limit of normal (IULN)
* AST(SGOT)/ALT(SGPT) ≤ 3.0 x IULN
* Creatinine ≤ IULN OR creatinine clearance ≥ 60 mL/min/1.73 m2 for patients with creatinine levels above institutional normal
* Systemic corticosteroid therapy is permitted provided dosing is no greater than 4 mg per day (dexamethasone or equivalent) on the day of vaccine administration.
* Bevacizumab will be allowed if given for symptomatic control of vasogenic edema and to avoid high dose of corticosteroids.
* Women of childbearing potential and men must agree to use adequate contraception (hormonal or barrier method of birth control, abstinence) prior to study entry and for the duration of study participation, including at least 5 months (for women of childbearing potential) and at least 7 months (for men) after last dose of study drug. Should a woman become pregnant or suspect she is pregnant while participating in this study, she must inform her treating physician immediately.
* Ability to understand and willingness to sign an IRB approved written informed consent document (or that of legally authorized representative, if applicable).

Exclusion Criteria

* As this study aims to assess the immunogenicity of various vaccine plus adjuvant combinations, no prior immunotherapy will be permitted.
* Inadequate tissue acquisition to allow for neoantigen screening.
* No candidate neoantigen identified during screening.
* A history of other malignancy ≤ 3 years previous with the exception of non-melanoma skin cancer, any in situ cancer that has been successfully resected and cured, treated superficial bladder cancer, or any early-stage solid tumor that was successfully resected without need for adjuvant radiation or chemotherapy.
* Receiving any other investigational agents within 4 weeks of beginning study treatment.
* Known allergy, or history of serious adverse reaction to, vaccines such as anaphylaxis, hives, or respiratory difficulty.
* A history of allergic reactions attributed to compounds of similar chemical or biologic composition to poly-ICLC or other agents used in the study.
* Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements.
* History of pre-existing immunodeficiency disorder or autoimmune condition requiring immunosuppressive therapy. This includes inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic vasculitis, scleroderma, psoriasis, multiple sclerosis, hemolytic anemia, immune-mediated thrombocytopenia, rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome, sarcoidosis, or other rheumatologic disease or any other medical condition or use of medication which might make it difficult for the patient to complete the full course of treatments or to generate an immune response to vaccines.
* Presence of clinically significant increased intracranial pressure (e.g. impending herniation) or hemorrhage, uncontrolled seizures, or requirement for immediate palliative treatment.
* Pregnant and/or breastfeeding. Women of childbearing potential must have a negative pregnancy test within 7 days of first dose of vaccine.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Bristol-Myers Squibb

INDUSTRY

Sponsor Role collaborator

Washington University School of Medicine

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Tanner Johanns, M.D., Ph.D.

Role: PRINCIPAL_INVESTIGATOR

Washington University School of Medicine

Locations

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Washington University School of Medicine

St Louis, Missouri, United States

Site Status

Countries

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United States

Provided Documents

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Document Type: Informed Consent Form

View Document

Related Links

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http://www.siteman.wustl.edu

Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine

Other Identifiers

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201804195

Identifier Type: -

Identifier Source: org_study_id