Trial Outcomes & Findings for Effect of Propolis or Metformin Administration on Glycemic Control in Patients With Type 2 Diabetes Mellitus (NCT NCT03416127)

NCT ID: NCT03416127

Last Updated: 2022-11-07

Results Overview

The fasting glucose levels were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the fasting glucose level at week 12

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

36 participants

Primary outcome timeframe

Week 12

Results posted on

2022-11-07

Participant Flow

Participant milestones

Participant milestones
Measure
Propolis
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Overall Study
STARTED
12
12
12
Overall Study
COMPLETED
12
11
11
Overall Study
NOT COMPLETED
0
1
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Propolis
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Overall Study
Withdrawal by Subject
0
1
1

Baseline Characteristics

Effect of Propolis or Metformin Administration on Glycemic Control in Patients With Type 2 Diabetes Mellitus

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=12 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=12 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Total
n=36 Participants
Total of all reporting groups
Age, Continuous
46 years
STANDARD_DEVIATION 7 • n=5 Participants
51 years
STANDARD_DEVIATION 4 • n=7 Participants
50 years
STANDARD_DEVIATION 5 • n=5 Participants
49 years
STANDARD_DEVIATION 6 • n=4 Participants
Sex: Female, Male
Female
4 Participants
n=5 Participants
7 Participants
n=7 Participants
7 Participants
n=5 Participants
18 Participants
n=4 Participants
Sex: Female, Male
Male
8 Participants
n=5 Participants
5 Participants
n=7 Participants
5 Participants
n=5 Participants
18 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
12 Participants
n=5 Participants
12 Participants
n=7 Participants
12 Participants
n=5 Participants
36 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Region of Enrollment
Mexico
12 participants
n=5 Participants
12 participants
n=7 Participants
12 participants
n=5 Participants
36 participants
n=4 Participants
Fasting serum glucose
7.3 mmol/L
STANDARD_DEVIATION 1.4 • n=5 Participants
8.0 mmol/L
STANDARD_DEVIATION 1.6 • n=7 Participants
7.7 mmol/L
STANDARD_DEVIATION 1.2 • n=5 Participants
7.7 mmol/L
STANDARD_DEVIATION 1.4 • n=4 Participants
2 hours after OGTT
14.6 mmol/L
STANDARD_DEVIATION 2.1 • n=5 Participants
15.8 mmol/L
STANDARD_DEVIATION 2.8 • n=7 Participants
13.2 mmol/L
STANDARD_DEVIATION 1.9 • n=5 Participants
14.5 mmol/L
STANDARD_DEVIATION 2.5 • n=4 Participants
Glycosylated Hemoglobin (A1C)
6.6 percentage
STANDARD_DEVIATION 0.8 • n=5 Participants
6.8 percentage
STANDARD_DEVIATION 0.9 • n=7 Participants
6.8 percentage
STANDARD_DEVIATION 0.9 • n=5 Participants
6.7 percentage
STANDARD_DEVIATION 0.8 • n=4 Participants
Total insulin secretion
0.5 index
STANDARD_DEVIATION 0.1 • n=5 Participants
0.4 index
STANDARD_DEVIATION 0.2 • n=7 Participants
0.5 index
STANDARD_DEVIATION 0.1 • n=5 Participants
0.5 index
STANDARD_DEVIATION 0.1 • n=4 Participants
Insulin sensitivity
1.0 index
STANDARD_DEVIATION 0.4 • n=5 Participants
1.1 index
STANDARD_DEVIATION 0.3 • n=7 Participants
0.8 index
STANDARD_DEVIATION 0.3 • n=5 Participants
1.0 index
STANDARD_DEVIATION 0.3 • n=4 Participants
First phase of insulin secretion
2092 index
STANDARD_DEVIATION 653 • n=5 Participants
1608 index
STANDARD_DEVIATION 736 • n=7 Participants
2221 index
STANDARD_DEVIATION 566 • n=5 Participants
1984 index
STANDARD_DEVIATION 686 • n=4 Participants
AUC glucose
965 mmol*min/L
STANDARD_DEVIATION 137 • n=5 Participants
1039 mmol*min/L
STANDARD_DEVIATION 174 • n=7 Participants
930 mmol*min/L
STANDARD_DEVIATION 90 • n=5 Participants
978 mmol*min/L
STANDARD_DEVIATION 133.6 • n=4 Participants
AUC insulin
52541 pmol*min/L
STANDARD_DEVIATION 9165 • n=5 Participants
45323 pmol*min/L
STANDARD_DEVIATION 9005 • n=7 Participants
53869 pmol*min/L
STANDARD_DEVIATION 5053 • n=5 Participants
50577.6 pmol*min/L
STANDARD_DEVIATION 7741 • n=4 Participants
Glucose 30 min after oral glucose tolerance test
12.4 mmol/L
STANDARD_DEVIATION 2.3 • n=5 Participants
13.7 mmol/L
STANDARD_DEVIATION 3.8 • n=7 Participants
12.0 mmol/L
STANDARD_DEVIATION 1.3 • n=5 Participants
12.7 mmol/L
STANDARD_DEVIATION 2.7 • n=4 Participants
Glucose 60 min after oral glucose tolerance test
15.2 mmol/L
STANDARD_DEVIATION 3.0 • n=5 Participants
17.2 mmol/L
STANDARD_DEVIATION 2.9 • n=7 Participants
14.7 mmol/L
STANDARD_DEVIATION 1.9 • n=5 Participants
15.6 mmol/L
STANDARD_DEVIATION 2.7 • n=4 Participants
Glucose 90 min after oral glucose tolerance test
14.7 mmol/L
STANDARD_DEVIATION 2.0 • n=5 Participants
17.5 mmol/L
STANDARD_DEVIATION 2.4 • n=7 Participants
14.3 mmol/L
STANDARD_DEVIATION 2.8 • n=5 Participants
15.5 mmol/L
STANDARD_DEVIATION 2.8 • n=4 Participants
Waist circumference
101.2 cm
STANDARD_DEVIATION 9.6 • n=5 Participants
103.4 cm
STANDARD_DEVIATION 11.7 • n=7 Participants
103.2 cm
STANDARD_DEVIATION 10.2 • n=5 Participants
102.6 cm
STANDARD_DEVIATION 10.3 • n=4 Participants
Body weight
76.4 kg
STANDARD_DEVIATION 11.5 • n=5 Participants
80.7 kg
STANDARD_DEVIATION 14.4 • n=7 Participants
78.6 kg
STANDARD_DEVIATION 13.1 • n=5 Participants
78.6 kg
STANDARD_DEVIATION 12.8 • n=4 Participants
Body mass index
29.0 kg/m^2
STANDARD_DEVIATION 4.4 • n=5 Participants
29.1 kg/m^2
STANDARD_DEVIATION 3.7 • n=7 Participants
30.2 kg/m^2
STANDARD_DEVIATION 3.13 • n=5 Participants
29.5 kg/m^2
STANDARD_DEVIATION 3.7 • n=4 Participants
Percentage of fat mass
32.8 % fat mass
STANDARD_DEVIATION 9.7 • n=5 Participants
30.7 % fat mass
STANDARD_DEVIATION 6.8 • n=7 Participants
33.8 % fat mass
STANDARD_DEVIATION 8.3 • n=5 Participants
32.4 % fat mass
STANDARD_DEVIATION 8.2 • n=4 Participants
Total cholesterol
5.0 mmol/L
STANDARD_DEVIATION 0.8 • n=5 Participants
4.7 mmol/L
STANDARD_DEVIATION 1.1 • n=7 Participants
4.7 mmol/L
STANDARD_DEVIATION 0.5 • n=5 Participants
4.8 mmol/L
STANDARD_DEVIATION 0.8 • n=4 Participants
Triglycerides
2.2 mmol/L
STANDARD_DEVIATION 1.1 • n=5 Participants
1.7 mmol/L
STANDARD_DEVIATION 0.6 • n=7 Participants
2.0 mmol/L
STANDARD_DEVIATION 1.0 • n=5 Participants
2.0 mmol/L
STANDARD_DEVIATION 0.9 • n=4 Participants
High Density Lipoprotein cholesterol (c-HDL)
1.0 mmol/L
STANDARD_DEVIATION 0.3 • n=5 Participants
0.9 mmol/L
STANDARD_DEVIATION 0.3 • n=7 Participants
0.9 mmol/L
STANDARD_DEVIATION 0.2 • n=5 Participants
0.9 mmol/L
STANDARD_DEVIATION 0.2 • n=4 Participants
Low-Density Lipoproteins-cholesterol (c-LDL)
3.0 mmol/L
STANDARD_DEVIATION 0.8 • n=5 Participants
3.0 mmol/L
STANDARD_DEVIATION 0.8 • n=7 Participants
2.9 mmol/L
STANDARD_DEVIATION 0.5 • n=5 Participants
3.0 mmol/L
STANDARD_DEVIATION 0.7 • n=4 Participants
Very low density lipoprotein (VLDL)
1.0 mmol/L
STANDARD_DEVIATION 0.5 • n=5 Participants
0.8 mmol/L
STANDARD_DEVIATION 0.3 • n=7 Participants
0.9 mmol/L
STANDARD_DEVIATION 0.5 • n=5 Participants
0.9 mmol/L
STANDARD_DEVIATION 0.4 • n=4 Participants
Creatinine
72.5 mmol/L
STANDARD_DEVIATION 31.8 • n=5 Participants
65.4 mmol/L
STANDARD_DEVIATION 21.2 • n=7 Participants
69.8 mmol/L
STANDARD_DEVIATION 27.4 • n=5 Participants
70.7 mmol/L
STANDARD_DEVIATION 26.5 • n=4 Participants
Uric acid
356.9 mmol/L
STANDARD_DEVIATION 71.4 • n=5 Participants
315.2 mmol/L
STANDARD_DEVIATION 65.4 • n=7 Participants
309.2 mmol/L
STANDARD_DEVIATION 89.2 • n=5 Participants
327.1 mmol/L
STANDARD_DEVIATION 77.3 • n=4 Participants
Systolic blood pressure
116.9 mmHg
STANDARD_DEVIATION 8.7 • n=5 Participants
118.4 mmHg
STANDARD_DEVIATION 10.6 • n=7 Participants
115.7 mmHg
STANDARD_DEVIATION 7.2 • n=5 Participants
117.0 mmHg
STANDARD_DEVIATION 8.8 • n=4 Participants
Diastolic blood pressure
76.5 mmHg
STANDARD_DEVIATION 7.8 • n=5 Participants
76.2 mmHg
STANDARD_DEVIATION 7.0 • n=7 Participants
73.4 mmHg
STANDARD_DEVIATION 6.7 • n=5 Participants
75.4 mmHg
STANDARD_DEVIATION 7.2 • n=4 Participants

PRIMARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The fasting glucose levels were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the fasting glucose level at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Fasting Serum Glucose
6.6 mmol/L
Standard Deviation 0.9
7.3 mmol/L
Standard Deviation 1.1
8.2 mmol/L
Standard Deviation 1.7

PRIMARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

2-h serum glucose levels were evaluated at week 12 after a oral glucose tolerance test with enzymatic/colorimetric techniques and the entered values reflect the insulin sensitivity at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
2 Hours After Oral Glucose Tolerance Test in Week 12
13.1 mmol/L
Standard Deviation 1.9
13.7 mmol/L
Standard Deviation 2.7
13.2 mmol/L
Standard Deviation 2.1

PRIMARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Glycosylated hemoglobin was evaluated at week 12 by high pressure liquid chromatography (HPLC) and the entered values reflect the glycosylated hemoglobin at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Glycosylated Hemoglobin (A1C)
6.3 % of A1C
Standard Deviation 0.9
6.6 % of A1C
Standard Deviation 0.8
7.0 % of A1C
Standard Deviation 1.0

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Total insulin secretion was calculated at week 12 with insulinogenic index and the entered values reflect the total insulin secretion at week 12. The insulinogenic index is a ratio that relates enhancement of circulating insulin to the magnitude of the corresponding glycemic stimulus. Total insulin secretion was calculated with the insulinogenic index (ΔAUC insulin/ΔAUC glucose), the entered values reflect the total insulin secretion

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Total Insulin Secretion
0.5 index
Standard Deviation 0.2
0.3 index
Standard Deviation 0.2
0.5 index
Standard Deviation 0.2

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Insulin sensitivity was calculated at week 12 with Matsuda index and the entered values reflect the insulin sensitivity at week 12. Matsuda Index value is used to indicate insulin resistance on diabetes. Insulin sensitivity was calculated with Matsuda index \[10,000 / √glucose 0' x insulin 0') (mean glucose oral glucose tolerance test (OGTT) x mean insulin OGTT)\]. The entered values reflect the insulin sensitivity

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Insulin Sensitivity
1.5 index
Standard Deviation 0.6
1.8 index
Standard Deviation 1.0
1.0 index
Standard Deviation 0.5

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The first phase of insulin secretion was calculated at week 12 with Stumvoll index and the entered values reflect the frst phase of insulin secretion at week 12. Human studies support the critical physiologic role of the first-phase of insulin secretion in the maintenance of postmeal glucose homeostasis. First phase of insulin secretion was estimated using the Stumvoll index (1283+ 1.829 x insulin 30' - 138.7 x glucose 30' + 3.772 x insulin 0'), the entered values reflect the first phase of insulin secretion

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
First Phase of Insulin Secretion
1346 index
Standard Deviation 784
996 index
Standard Deviation 742
1652 index
Standard Deviation 863

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Area under the curve (AUC) of glucose was calculated with the polygonal formula. The area under the curve (AUC) of glucose, (0.5 \* glucose (G) 0´ + (G 30´+G 60´ + G 90´) + 0.5 \* G 120´) \* 30; has been widely used for calculating the glycemic index and for evaluating the efficacy of medications for postprandial hyperglycemia.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
AUC Glucose
921 mmol*min/L
Standard Deviation 126
967 mmol*min/L
Standard Deviation 116
939 mmol*min/L
Standard Deviation 126.4

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Area under the curve (AUC) of insulin was calculated with the polygonal formula. The area under the curve (AUC) of insulin, (0.5 \* Insulin (I) 0´ + (I 30´+I 60´ + I 90´) + 0.5 \* I 120´) \* 30; has been widely used for calculating the glycemic index and for evaluating the efficacy of medications for postprandial hyperinsulinemia.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
AUC Insulin
44123 pmol*min/L
Standard Deviation 13381
27603 pmol*min/L
Standard Deviation 15935
51287 pmol*min/L
Standard Deviation 7440

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The glucose levels 30' were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the glucose 30' at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Glucose 30 Min After Oral Glucose Tolerance Test at 12 Week
12.3 mmol/L
Standard Deviation 2.0
12.5 mmol/L
Standard Deviation 2.1
12.0 mmol/L
Standard Deviation 1.8

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The glucose levels 60' were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the glucose 60' at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Glucose 60 Min After Oral Glucose Tolerance Test at 12 Week
15.1 mmol/L
Standard Deviation 2.8
15.9 mmol/L
Standard Deviation 2.5
14.2 mmol/L
Standard Deviation 2.6

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The glucose levels 90' were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the glucose 90' at week 12.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Glucose 90 Min After Oral Glucose Tolerance Test at 12 Week
14.2 mmol/L
Standard Deviation 2.6
16.6 mmol/L
Standard Deviation 2.1
14.9 mmol/L
Standard Deviation 2.9

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Waist circumference was evaluated at week 12 with a flexible tape

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Waist Circumference
100.7 cm
Standard Deviation 10.0
102.2 cm
Standard Deviation 11.9
101.5 cm
Standard Deviation 10.2

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The body weight was measured at week 12 with a bioimpedance balance and the entered values reflect the body weight at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Body Weight
75.3 kg
Standard Deviation 11.3
81.0 kg
Standard Deviation 15.2
77.8 kg
Standard Deviation 13.5

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Body Mas Index was calculated at week 12 with the Quetelet index formula and the entered values reflect the body mass index at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Body Mass Index
28.6 kg/m2
Standard Deviation 4.3
29.2 kg/m2
Standard Deviation 3.8
30.0 kg/m2
Standard Deviation 2.9

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Percentage of fat mass was evaluated through bioimpedance.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Percentage of Fat Mass
32.8 % of fat mass
Standard Deviation 10.3
28.4 % of fat mass
Standard Deviation 6.8
33.6 % of fat mass
Standard Deviation 8.2

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Total cholesterol levels were evaluated at week 12 by enzymatic/colorimetric techniques and the entered values reflect the total cholesterol level at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Total Cholesterol
5.1 mmol/L
Standard Deviation 0.7
4.6 mmol/L
Standard Deviation 0.9
4.7 mmol/L
Standard Deviation 0.7

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Triglycerides levels were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the triglycerides level at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Triglycerides
2.0 mmol/L
Standard Deviation 0.8
2.0 mmol/L
Standard Deviation 1.0
1.9 mmol/L
Standard Deviation 0.8

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

c-HDL levels were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the c-HDL level at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
High Density Lipoprotein Cholesterol (c-HDL)
1.0 mmol/L
Standard Deviation 0.3
0.9 mmol/L
Standard Deviation 0.2
0.9 mmol/L
Standard Deviation 0.1

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

c-LDL levels were evaluated at week 12 with enzymatic/colorimetric techniques and the entered values reflect the c-LDL level at week 12

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Low Density Lipoproteins Cholesterol (c-LDL)
3.1 mmol/L
Standard Deviation 0.5
2.7 mmol/L
Standard Deviation 0.5
3.0 mmol/L
Standard Deviation 0.5

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Very low density lipoprotein (c-VLDL) was analyzed with the cholesterol oxidase/peroxidase technique by enzymatic colorimetric methods on an automatic analyzer.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Very Low Density Lipoprotein (c-VLDL)
0.9 mmol/L
Standard Deviation 0.4
0.9 mmol/L
Standard Deviation 0.5
0.9 mmol/L
Standard Deviation 0.4

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Creatinine levels were evaluated at week 12 with enzymatic/colorimetric techniques

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Creatinine
55.7 mmol/L
Standard Deviation 10.6
74.2 mmol/L
Standard Deviation 16.8
63.6 mmol/L
Standard Deviation 26.5

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

Uric acid levels were evaluated at week 12 with enzymatic/colorimetric techniques

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Uric Acid
339.0 mmol/L
Standard Deviation 77.3
327.1 mmol/L
Standard Deviation 71.4
333.1 mmol/L
Standard Deviation 95.2

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The systolic blood pressure was evaluated at week 12 with a digital sphygmomanometer and the entered values reflect the systolic blood pressure at week 12.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Systolic Blood Pressure
114.6 mmHg
Standard Deviation 7.4
119.2 mmHg
Standard Deviation 11.4
112.8 mmHg
Standard Deviation 6.4

SECONDARY outcome

Timeframe: Week 12

Population: Two of the study groups have one less patient due to withdrawal by subject, as mentioned in the participant flow, so the analysis of this outcome is calculated with those who finished the intervention

The diastolic blood pressure was evaluated at week 12 with a digital sphygmomanometer and the entered values reflect the diastolic blood pressure at week 12.

Outcome measures

Outcome measures
Measure
Propolis
n=12 Participants
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=11 Participants
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=11 Participants
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Diastolic Blood Pressure
74.8 mmHg
Standard Deviation 7.4
74.4 mmHg
Standard Deviation 7.9
72.3 mmHg
Standard Deviation 3.8

Adverse Events

Propolis

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Metformin

Serious events: 0 serious events
Other events: 3 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 5 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Propolis
n=12 participants at risk
Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks. Propolis: Propolis capsules, 300 mg, two times per day before break-fast and dinner during 12 weeks.
Metformin
n=12 participants at risk
Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks. Metformin: Metformin capsules, 850 mg, two times per day before break-fast and dinner during 12 weeks.
Placebo
n=12 participants at risk
Placebo capsules, two times per day before break-fast and dinner during 12 weeks. Placebo: Calcined magnesium, two times per day before break-fast and dinner during 12 weeks.
Gastrointestinal disorders
Diarrhea
0.00%
0/12 • Throughout the study, an average of 12 weeks
8.3%
1/12 • Number of events 1 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
Gastrointestinal disorders
Loose stools
0.00%
0/12 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
16.7%
2/12 • Number of events 2 • Throughout the study, an average of 12 weeks
Gastrointestinal disorders
Nausea
0.00%
0/12 • Throughout the study, an average of 12 weeks
8.3%
1/12 • Number of events 1 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
Metabolism and nutrition disorders
Hyperphagia
0.00%
0/12 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
16.7%
2/12 • Number of events 2 • Throughout the study, an average of 12 weeks
Nervous system disorders
Headache
8.3%
1/12 • Number of events 1 • Throughout the study, an average of 12 weeks
8.3%
1/12 • Number of events 1 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
Gastrointestinal disorders
Constipation
0.00%
0/12 • Throughout the study, an average of 12 weeks
0.00%
0/12 • Throughout the study, an average of 12 weeks
8.3%
1/12 • Number of events 1 • Throughout the study, an average of 12 weeks

Additional Information

DR. MANUEL GONZALEZ ORTIZ

INSTITUTO DE TERAPEUTICA EXPERIMENTAL Y CLINICA

Phone: +52 3310585200

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place