Trial Outcomes & Findings for Immunologic Determinants of Response to Pembrolizumab (MK-3475) in Advanced Melanoma (MK-3475-161/KEYNOTE-161) (NCT NCT03407170)

NCT ID: NCT03407170

Last Updated: 2020-07-15

Results Overview

FCT is defined as the fraction of CD8+ T-cells expressing a predefined single-cell ribonucleic acid (RNA) gene signature to the total tumor infiltrating CD8+T-cells isolated from tumor biopsies.

Recruitment status

TERMINATED

Study phase

PHASE2

Target enrollment

1 participants

Primary outcome timeframe

Up to approximately 59 weeks

Results posted on

2020-07-15

Participant Flow

Participant milestones

Participant milestones
Measure
Pembrolizumab
Participants received pembrolizumab 200 mg by intravenous (IV) infusion every 3 weeks (Q3W).
Overall Study
STARTED
1
Overall Study
COMPLETED
0
Overall Study
NOT COMPLETED
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Pembrolizumab
Participants received pembrolizumab 200 mg by intravenous (IV) infusion every 3 weeks (Q3W).
Overall Study
Adverse Event
1

Baseline Characteristics

Immunologic Determinants of Response to Pembrolizumab (MK-3475) in Advanced Melanoma (MK-3475-161/KEYNOTE-161)

Baseline characteristics by cohort

Baseline data not reported

PRIMARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

FCT is defined as the fraction of CD8+ T-cells expressing a predefined single-cell ribonucleic acid (RNA) gene signature to the total tumor infiltrating CD8+T-cells isolated from tumor biopsies.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

ASCTFR is defined as the arithmetic average of the log10 ratio of the frequency of individual specific cytotoxic T-Cell Receptor (TCR) clones of on-treatment to pre-treatment.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

The fold change from baseline in FCT. FCT is defined as the fraction of CD8+ T-cells expressing a predefined single-cell RNA gene signature to the total tumor infiltrating CD8+T-cells isolated from tumor biopsies.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: The analysis population consisted of all participants who received ≥1 dose of study treatment.

An AE is any untoward medical occurrence in a participant temporally associated with the use of study treatment, whether or not considered related to the study treatment. The number of participants who experienced an AE is presented.

Outcome measures

Outcome measures
Measure
Pembrolizumab
n=1 Participants
Participants received pembrolizumab 200 mg by IV infusion Q3W.
Number of Participants Who Experienced an Adverse Event (AE)
1 Participants

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: The analysis population consisted of all participants who received ≥1 dose of study treatment.

The number of participants who discontinued any study treatment due to an AE is presented.

Outcome measures

Outcome measures
Measure
Pembrolizumab
n=1 Participants
Participants received pembrolizumab 200 mg by IV infusion Q3W.
Number of Participants Who Discontinued Any Study Drug Due to an Adverse Event (AE)
1 Participants

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

ORR is defined as the percentage of participants in the analysis population who have a Complete Response (CR: Disappearance of all target lesions) or a Partial Response (PR: ≥30% decrease in the sum of diameters of target lesions) per RECIST Version 1.1 as assessed by the investigator.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

PFS is defined as the time from start of treatment to the first documented progressive disease (PD) or death due to any cause, whichever occurs first, per RECIST Version 1.1 as assessed by the investigator.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

OS is defined as the time from the start of treatment to death due to any cause.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

Neoepitope sequencing will be generated based on single cell ribonucleic acid (RNA) sequencing (scRNAseq), whole exome sequencing, and an epitope prediction algorithm to obtain neoepitope burden.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to approximately 59 weeks

Population: No data were collected or analyzed for this outcome measure due to early termination of the study.

T-cell receptors (TCRs) from CD8+ T-cell clones will be identified by single cell ribonucleic acid (RNA) sequencing (scRNAseq) and their killing function will be confirmed by TCR-transduced T-cells recognizing autologous tumor-derived cell lines.

Outcome measures

Outcome data not reported

Adverse Events

Pembrolizumab

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Senior Vice President, Global Clinical Development

Merck Sharp & Dohme Corp.

Phone: 1-800-672-6372

Results disclosure agreements

  • Principal investigator is a sponsor employee The Sponsor will generally support publication of multicenter studies only in their entirety and not as individual site data. In this case, a coordinating investigator will be designated by mutual agreement. If publication activity is not directed by the Sponsor, the investigator agrees to submit all manuscripts or abstracts to the Sponsor before submission. This allows the Sponsor to protect proprietary information and to provide comments.
  • Publication restrictions are in place

Restriction type: OTHER