Trial Outcomes & Findings for Assessing the Endometrial Environment in Recurrent Pregnancy Loss and Unexplained Infertility (NCT NCT03401918)

NCT ID: NCT03401918

Last Updated: 2025-04-01

Results Overview

The primary outcome will abnormal microbiome results. An abnormal microbiome will be defined as less then 90% lactobacilli on DNA sequencing

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

41 participants

Primary outcome timeframe

1 month

Results posted on

2025-04-01

Participant Flow

Participant milestones

Participant milestones
Measure
Patients With Recurrent Pregnancy Loss or Unexplained Infertility
Patients with recurrent pregnancy loss or unexplained infertility will have an assessment of the uterine environment at the time of implantation, followed by testing of uterine endometrial gene expression using the ERA test and the uterine micro biome. Those who have abnormal results (an abnormal microbiome or an abnormal ERA) will have the option to undergo treatment followed by retesting of the uterine environment. For an abnormal ERA suggesting a pre-receptive result, luteal phase vaginal progesterone supplementation will be offered prior to re-testing of the ERA. For an abnormal microbiome a combination of oral antibiotics and vaginal probiotics will be offered prior to re-testing the uterine microbiome.
Healthy Control Patients
Patients who have had a normal delivery and no history of infertility or recurrent pregnancy loss will have assessment of the uterine environment at the time of implantation.
Overall Study
STARTED
25
16
Overall Study
COMPLETED
25
16
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Assessing the Endometrial Environment in Recurrent Pregnancy Loss and Unexplained Infertility

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Patients With Recurrent Pregnancy Loss or Unexplained Infertility
n=25 Participants
Patients with recurrent pregnancy loss or unexplained infertility will have an assessment of the uterine environment at the time of implantation, followed by testing of uterine endometrial gene expression using the ERA test and the uterine micro biome. Those who have abnormal results (an abnormal microbiome or an abnormal ERA) will have the option to undergo treatment followed by retesting of the uterine environment. For an abnormal ERA suggesting a pre-receptive result, luteal phase vaginal progesterone supplementation will be offered prior to re-testing of the ERA. For an abnormal microbiome a combination of oral antibiotics and vaginal probiotics will be offered prior to re-testing the uterine microbiome.
Healthy Control Patients
n=16 Participants
Patients who have had a normal delivery and no history of infertility or recurrent pregnancy loss will have assessment of the uterine environment at the time of implantation.
Total
n=41 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
25 Participants
n=5 Participants
16 Participants
n=7 Participants
41 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Sex: Female, Male
Female
25 Participants
n=5 Participants
16 Participants
n=7 Participants
41 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race/Ethnicity, Customized
Asian
13 Participants
n=5 Participants
2 Participants
n=7 Participants
15 Participants
n=5 Participants
Race/Ethnicity, Customized
Caucasian
12 Participants
n=5 Participants
12 Participants
n=7 Participants
24 Participants
n=5 Participants
Race/Ethnicity, Customized
Black
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Race/Ethnicity, Customized
Hispanic
0 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
Region of Enrollment
United States
25 Participants
n=5 Participants
16 Participants
n=7 Participants
41 Participants
n=5 Participants

PRIMARY outcome

Timeframe: 1 month

Population: No participants were analyzed because the assay failed validation and the results are uninterpretable.

The primary outcome will abnormal microbiome results. An abnormal microbiome will be defined as less then 90% lactobacilli on DNA sequencing

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: 1 month

Population: No endometrial receptivity array data were collected because the microbiome assay failed validation and further testing was not done.

The second primary outcome will be abnormal endometrial receptivity array defined as non-receptive (pre or post receptive) based on RNA sequencing of an endometrial gene array.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: 3-6 months

Population: Data were not collected for this outcome measure as the study did not continue to the treatment phase.

The primary outcome will be normal microbiome results defined as \>90% lactobacilli after oral antibiotics and vaginal probiotics after initially abnormal microbiome results

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: 3-6 months

Population: Data were not collected for this outcome measure as the study did not continue to the treatment phase.

The primary outcome will be normal endometrial receptivity array results defined as receptive after luteal phase progesterone support after initially abnormal endometrial receptivity array results

Outcome measures

Outcome data not reported

Adverse Events

Patients With Recurrent Pregnancy Loss or Unexplained Infertility

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Healthy Control Patients

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Ruth Bunker Lathi

Stanford University

Phone: (650) 498-7911

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place