HER2 Directed Dendritic Cell Vaccine During Neoadjuvant Therapy of HER2+Breast Cancer

NCT ID: NCT03387553

Last Updated: 2025-11-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

EARLY_PHASE1

Total Enrollment

31 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-06-06

Study Completion Date

2026-08-29

Brief Summary

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The purpose of this study is to learn more about how to treat patients with HER-2/neu positive invasive breast cancer (IBC). HER-2/neu is a type of protein that is known to be over-expressed in aggressive breast cancer.

The study drug for this trial is DC1 study vaccine which is a HER2-sensitized dendritic cell (DC) study vaccine. This study vaccine is made from the participant's blood cells collected from a procedure called leukapheresis. Dendritic cells are immune cells that can tell the immune system to fight infection. In laboratory testing and from previous studies in participants, these cells may also help the immune system attack tumors such as breast cancer.

Detailed Description

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The trial will consist of two phases. The first lead in phase will enroll 12 participants evenly divided into two arms (alternating enrollment) with different initial priming study vaccination schedules.

Arm A: One DC1 study vaccination per week x 3 weeks. Arm B: Two DC1 study vaccinations per week (given 3 days apart for example Mon and Thurs or Tues and Friday) x 3 weeks.

Following accrual of this initial group of 12 participants, HER2 ELISPOT post study vaccination responses will be assessed to determine which of the two sequences provides the greater increase in anti HER2 response at week 4 over baseline. This will determine which sequence will be used in the second expansion phase of accrual. If both arms are determined equal then Arm A will be selected as the default sequence.

Second phase of accrual will consist of 14 additional participants to undergo study vaccination using the optimal schedule declared in the first phase of the trial. The trial will consist of two phases. The first lead in phase will enroll 12 participants evenly divided into two arms (alternating enrollment) with different initial priming study vaccination schedules.

Arm A: One DC1 study vaccination per week x 3 weeks Arm B: Two DC1 study vaccinations per week (given 3 days apart for example Mon and Thurs or Tues and Friday) x 3 weeks.

Following accrual of this initial group of 12 participants, HER2 ELISPOT post study vaccination responses will be assessed to determine which of the two sequences provides the greater increase in anti HER2 response at week 4 over baseline. This will determine which sequence will be used in the second expansion phase of accrual. If both arms are determined equal then Arm A will be selected as the default sequence.

Second phase of accrual will consist of 14 additional participants to undergo study vaccination using the optimal schedule declared in the first phase of the trial.

Conditions

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Breast Cancer Breast Cancer Female Breast Cancer, Male Invasive Breast Cancer HER2-positive Breast Cancer HER2 Positive Breast Carcinoma Stage II Breast Cancer Stage III Breast Cancer

Keywords

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Immunotherapy Dendritic Cell Vaccine DC1

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

The trial will consist of two phases. The first lead in phase will enroll 12 participants evenly divided into two arms (alternating enrollment) with different initial priming vaccination schedules.

Following accrual of this initial group of 12 patients, HER2 ELISPOT post vaccination responses will be assessed to determine which of the two sequences provides the greater increase in anti HER2 response at week 4 over baseline. This will determine which sequence will be used in the second expansion phase of accrual. If both arms are determined equal then Arm A will be selected as the default sequence.
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Lead In Phase - Arm A

Arm A: One Dendritic Cell Vaccine (DC1) per week x 3 weeks.

Group Type ACTIVE_COMPARATOR

Neoadjuvant Chemotherapy

Intervention Type DRUG

Upon completion of the 3 week series of vaccinations participants will then undergo neoadjuvant chemotherapy treatment with the TCH-P Taxotere (docetaxel), Carboplatin, Herceptin (trastuzumab), Perjeta (pertuzumab) standard of care neoadjuvant chemotherapy regimen given intravenously once every 3 weeks for up to 6 cycles. The treating physician will have the discretion to delay, modify, or shorten the neoadjuvant chemotherapy as per routine practice guidelines and physician discretion.

Curative Surgery

Intervention Type PROCEDURE

Planned definitive curative surgery at 26 to 28 weeks.

Dendritic Cell Vaccine (DC1)

Intervention Type BIOLOGICAL

Study Vaccine:

Lead In Phase - Weekly as outlined in each treatment Arm.

Expansion Phase - At the optimal schedule determined at the end of the Lead In Phase.

Pre-surgery - Booster Vaccine at week 25 prior to receiving surgery.

Post-surgery - Participants will receive a series of 3 booster intranodal study vaccines given once every 6 months.

Lead In Phase - Arm B

Arm B: Two DC1 vaccinations per week (given 3 days apart i.e., Mon and Thurs or Tues and Friday) x 3 weeks.

Group Type ACTIVE_COMPARATOR

Neoadjuvant Chemotherapy

Intervention Type DRUG

Upon completion of the 3 week series of vaccinations participants will then undergo neoadjuvant chemotherapy treatment with the TCH-P Taxotere (docetaxel), Carboplatin, Herceptin (trastuzumab), Perjeta (pertuzumab) standard of care neoadjuvant chemotherapy regimen given intravenously once every 3 weeks for up to 6 cycles. The treating physician will have the discretion to delay, modify, or shorten the neoadjuvant chemotherapy as per routine practice guidelines and physician discretion.

Curative Surgery

Intervention Type PROCEDURE

Planned definitive curative surgery at 26 to 28 weeks.

Dendritic Cell Vaccine (DC1)

Intervention Type BIOLOGICAL

Study Vaccine:

Lead In Phase - Weekly as outlined in each treatment Arm.

Expansion Phase - At the optimal schedule determined at the end of the Lead In Phase.

Pre-surgery - Booster Vaccine at week 25 prior to receiving surgery.

Post-surgery - Participants will receive a series of 3 booster intranodal study vaccines given once every 6 months.

Expansion Phase

DC1 vaccinations according to optimal vaccination schedule. Participants will receive a booster intranodal study vaccine at week 25 prior to receiving surgery. Participants will then undergo definitive curative surgery following completion of the neoadjuvant therapy, additional adjuvant locoregional/systemic therapy (as deemed appropriate by their treating physicians).

Group Type EXPERIMENTAL

Neoadjuvant Chemotherapy

Intervention Type DRUG

Upon completion of the 3 week series of vaccinations participants will then undergo neoadjuvant chemotherapy treatment with the TCH-P Taxotere (docetaxel), Carboplatin, Herceptin (trastuzumab), Perjeta (pertuzumab) standard of care neoadjuvant chemotherapy regimen given intravenously once every 3 weeks for up to 6 cycles. The treating physician will have the discretion to delay, modify, or shorten the neoadjuvant chemotherapy as per routine practice guidelines and physician discretion.

Curative Surgery

Intervention Type PROCEDURE

Planned definitive curative surgery at 26 to 28 weeks.

Dendritic Cell Vaccine (DC1)

Intervention Type BIOLOGICAL

Study Vaccine:

Lead In Phase - Weekly as outlined in each treatment Arm.

Expansion Phase - At the optimal schedule determined at the end of the Lead In Phase.

Pre-surgery - Booster Vaccine at week 25 prior to receiving surgery.

Post-surgery - Participants will receive a series of 3 booster intranodal study vaccines given once every 6 months.

Interventions

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Neoadjuvant Chemotherapy

Upon completion of the 3 week series of vaccinations participants will then undergo neoadjuvant chemotherapy treatment with the TCH-P Taxotere (docetaxel), Carboplatin, Herceptin (trastuzumab), Perjeta (pertuzumab) standard of care neoadjuvant chemotherapy regimen given intravenously once every 3 weeks for up to 6 cycles. The treating physician will have the discretion to delay, modify, or shorten the neoadjuvant chemotherapy as per routine practice guidelines and physician discretion.

Intervention Type DRUG

Curative Surgery

Planned definitive curative surgery at 26 to 28 weeks.

Intervention Type PROCEDURE

Dendritic Cell Vaccine (DC1)

Study Vaccine:

Lead In Phase - Weekly as outlined in each treatment Arm.

Expansion Phase - At the optimal schedule determined at the end of the Lead In Phase.

Pre-surgery - Booster Vaccine at week 25 prior to receiving surgery.

Post-surgery - Participants will receive a series of 3 booster intranodal study vaccines given once every 6 months.

Intervention Type BIOLOGICAL

Other Intervention Names

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Immunotherapy TCHP

Eligibility Criteria

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Inclusion Criteria

* Participants must have histologically confirmed clinical stage II or III ERPR- HER2+ (per CAP criteria) invasive carcinoma of the breast
* Medically and surgically appropriate to undergo neoadjuvant chemotherapy with TCH-P Taxotere (docetaxel), Carboplatin, Herceptin (trastuzumab), Perjeta (pertuzumab) regimen followed by standard of care local therapy as determined by their treating physician
* Age ≥18 years.
* Eastern Cooperative Oncology Group (ECOG) performance status less than 2
* Patients must have normal organ and marrow function as defined below:

* leukocytes ≥3,000/μL
* absolute neutrophil count ≥1,500/μL
* platelets ≥100,000/μL
* total bilirubin within normal institutional limits
* AST(SGOT)/ALT(SGPT) less than or equal to 2.5 X institutional upper limit of normal
* creatinine within normal institutional limits - OR -
* creatinine clearance ≥60 mL/min/1.73 m\^2 for patients with creatinine levels above institutional normal
* Cardiac ejection fraction within institutional normal limits by either MUGA or ECHO at baseline.
* Women of child-bearing potential and their male partners must agree to use adequate contraception (hormonal or barrier method of birth control; abstinence) prior to study entry and for the duration of study participation. Should a woman become pregnant or suspect she is pregnant while participating in this study, she should inform her treating physician immediately. Sexually active male participants should use a barrier method or exercise abstinence during chemotherapy administration until surgery.
* Ability to understand and the willingness to sign a written informed consent document.

Exclusion Criteria

* Patients with inflammatory breast cancer, widespread locally advanced unresectable disease involving the chest wall/nodal basins in which a curative surgical resection cannot be performed, or those in whom de novo metastatic disease is suspected or confirmed
* May not be receiving any other investigational agents for the treatment of their breast cancer.
* History of allergic reactions attributed to compounds of similar chemical or biologic composition to the study vaccine components and any of the chemotherapy drugs (docetaxel, carboplatin, trastuzumab, pertuzumab)
* Unwilling or unable to undergo an apheresis for production of their vaccine
* Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements.
* Women who are pregnant or breastfeeding
* Known congenital or acquired immune deficiency (including those patients who require systemic immunosuppressant drugs for autoimmune disease or organ transplant).
* Pre-existing peripheral neuropathy that would limit treatment with taxanes and platinum agents
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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United States Department of Defense

FED

Sponsor Role collaborator

H. Lee Moffitt Cancer Center and Research Institute

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Haten Soliman, M.D.

Role: PRINCIPAL_INVESTIGATOR

H. Lee Moffitt Cancer Center and Research Institute

Locations

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H. Lee Moffitt Cancer Center and Research Institute

Tampa, Florida, United States

Site Status

Countries

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United States

References

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Soliman H, Aldrich A, Abdo N, Han H, Soyano A, Costa R, Armaghani A, Kiluk J, Khakpour N, Lee MC, Hoover S, Laronga C, Niell B, Mooney B, Weinfurtner RJ, Rosa M, Czerniecki B. A pilot study incorporating HER2-directed dendritic cells into neoadjuvant therapy of early stage HER2+ER- breast cancer. NPJ Breast Cancer. 2025 Mar 17;11(1):29. doi: 10.1038/s41523-025-00742-x.

Reference Type DERIVED
PMID: 40097486 (View on PubMed)

Related Links

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https://moffitt.org/clinical-trials-research/

Moffitt Cancer Center Clinical Trials website

Other Identifiers

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MCC-19337

Identifier Type: -

Identifier Source: org_study_id