Evaluating the Infectivity, Safety, and Immunogenicity of a Respiratory Syncytial Virus Vaccine (RSV 6120/∆NS2/1030s) in RSV-Seropositive Children and RSV-Seronegative Infants and Children

NCT ID: NCT03387137

Last Updated: 2024-12-11

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

45 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-10-13

Study Completion Date

2021-05-31

Brief Summary

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The purpose of this study is to evaluate the infectivity, safety, and immunogenicity of a single dose of a recombinant, live-attenuated respiratory syncytial virus (RSV) vaccine (RSV 6120/∆NS2/1030s) in RSV-seropositive children 12 to 59 months of age and RSV-seronegative infants and children 6 to 24 months of age.

Detailed Description

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This study will evaluate the infectivity, safety, and immunogenicity of a single dose of a recombinant, live-attenuated RSV vaccine (RSV 6120/∆NS2/1030s) in RSV-seropositive children 12 to 59 months of age and RSV-seronegative infants and children 6 to 24 months of age.

The vaccine will be evaluated in a stepwise fashion beginning in RSV-seropositive children (Group 1), and then in RSV-seronegative infants and children (Group 2). In each group, participants will be randomly assigned to receive a single dose of RSV 6120/∆NS2/1030s vaccine or placebo at study entry (Day 0).

Participants will be enrolled in the study between April 1 and October 31, outside of the RSV season. Group 1 (RSV-seropositive children) will be followed for 28 days. Group 2 (RSV-seronegative infants and children) will remain on study until they complete the post-RSV season visit.

Group 2 participants may participate in an optional second season of RSV surveillance during November to March of the second year following enrollment.

Study visits for all participants may include clinical assessments, blood collection, and nasal washes. Additionally, participants' parents or guardians will be contacted by study staff at various times during the study to monitor participants' health.

Conditions

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RSV Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Group 1: RSV 6120/∆NS2/1030s Vaccine

RSV-seropositive children will receive a single dose of 10\^5.7 plaque-forming units (PFUs) of RSV 6120/∆NS2/1030s vaccine at study entry (Day 0).

Group Type EXPERIMENTAL

RSV 6120/∆NS2/1030s

Intervention Type BIOLOGICAL

Delivered as nose drops

Group 1: Placebo

RSV-seropositive children will receive a single dose of placebo at study entry (Day 0).

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Delivered as nose drops

Group 2: RSV 6120/∆NS2/1030s Vaccine

RSV-seronegative infants and children will receive a single dose of 10\^5.0 PFUs of RSV 6120/∆NS2/1030s vaccine at study entry (Day 0).

Group Type EXPERIMENTAL

RSV 6120/∆NS2/1030s

Intervention Type BIOLOGICAL

Delivered as nose drops

Group 2: Placebo

RSV-seronegative infants and children will receive a single dose of placebo at study entry (Day 0).

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Delivered as nose drops

Interventions

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RSV 6120/∆NS2/1030s

Delivered as nose drops

Intervention Type BIOLOGICAL

Placebo

Delivered as nose drops

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Greater than or equal to 12 months of age and less than 60 months of age at the time of inoculation
* Screening serum specimen for RSV neutralizing antibody is obtained within the calendar year of inoculation
* Seropositive for RSV antibody, defined as serum RSV neutralizing antibody titer greater than or equal to 1:40
* Pre-inoculation serum sample for RSV neutralizing antibody testing is obtained no more than 56 days prior to inoculation
* In good health based on review of the medical record, history, and physical examination at the time of inoculation
* Received routine immunizations appropriate for age based on the Advisory Committee on Immunization Practices (ACIP) Recommended Immunization Schedule for Children and Adolescents Aged 18 Years or Younger
* Growing normally for age as demonstrated on a standard growth chart and has a current height and weight above the 3rd percentile for age
* Expected to be available for the duration of the study
* Parent/guardian is willing and able to provide written informed consent


* Greater than or equal to 6 months of age and less than 25 months of age at the time of inoculation
* Screening and pre-inoculation serum specimens for RSV-neutralizing antibody are obtained no more than 42 days prior to inoculation
* Seronegative for RSV antibody, defined as serum RSV-neutralizing antibody titer less than 1:40
* In good health based on review of the medical record, history, and physical examination at the time of inoculation
* Received routine immunizations appropriate for age based on the ACIP Recommended Immunization Schedule for Children and Adolescents Aged 18 Years or Younger
* Growing normally for age as demonstrated on a standard growth chart, AND

* If less than 1 year of age: has a current height and weight above the 5th percentile for age
* If greater than or equal to 1 year of age: has a current height and weight above the 3rd percentile for age
* Expected to be available for the duration of the study
* Parent/guardian is willing and able to provide written informed consent


* RSV-seronegative participants who have completed the initial RSV season surveillance portion of the study and whose parent/guardian is willing and able to provide written informed consent
* Expected to be available during the second year of RSV surveillance

Exclusion Criteria

* Born at less than 34 weeks gestation
* Maternal history of positive HIV test
* Evidence of chronic disease
* Known or suspected impairment of immune function
* Bone marrow/solid organ transplant recipient
* Major congenital malformations, including congenital cleft palate or cytogenetic abnormalities
* Suspected or documented developmental disorder, delay, or other developmental problem
* Cardiac abnormality requiring treatment
* Lung disease or reactive airway disease
* More than one episode of wheezing in the first year of life
* Wheezing episode or received bronchodilator therapy within the past 12 months
* Wheezing episode or received bronchodilator therapy after the age of 12 months
* Previous receipt of supplemental oxygen therapy in a home setting
* Previous receipt of an investigational RSV vaccine
* Previous receipt or planned administration of anti-RSV drug (ribavirin) or RSV antibody product including RSV immune globulin (RSV Ig) or RSV monoclonal antibody (RSV mAb)
* Previous receipt of immunoglobulin or any antibody products within the past 6 months
* Previous receipt of any other blood products within the past 6 months
* Previous anaphylactic reaction
* Previous vaccine-associated adverse reaction that was Grade 3 or above
* Known hypersensitivity to any vaccine component
* Member of a household that contains an infant who is less than 12 months of age at the date of inoculation through the 10th day after inoculation
* Member of a household that, at the date of inoculation through the 10th day after inoculation, contains an immunocompromised individual including but not limited to:

* a person who is HIV-infected
* a person who has received chemotherapy within the 12 months prior to enrollment
* a person receiving immunosuppressant agents
* a person living with a solid organ or bone marrow transplant
* Will attend a daycare facility that does not separate children by age and contains an infant who is less than 12 months of age at the date of inoculation through the 10th day after inoculation
* Receipt of any of the following prior to enrollment:

* any inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days prior, or
* any live vaccine, other than rotavirus vaccine, within the 28 days prior, or
* another investigational vaccine or investigational drug within 28 days prior, or
* salicylate (aspirin) or salicylate-containing products within the past 28 days
* Scheduled administration of any of the following after planned inoculation:

* inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days after, or
* any live vaccine other than rotavirus in the 28 days after, or
* another investigational vaccine or investigational drug in the 28 days after
* Receipt of any of the following medications within 3 days of study enrollment:

* systemic antibacterial, antiviral, antifungal, anti-parasitic, or antituberculous agents, whether for treatment or prophylaxis, or
* intranasal medications, or
* other prescription medications except the permitted concomitant medications listed below:
* Permitted concomitant medications (prescription or non-prescription) include nutritional supplements, medications for gastroesophageal reflux, eye drops, and topical medications, including (but not limited to) cutaneous (topical) steroids, topical antibiotics, and topical antifungal agents.
* Any of the following events at the time of enrollment:

* fever (temporal or rectal temperature of greater than or equal to 100.4°F), or
* upper respiratory signs or symptoms (rhinorrhea, cough, or pharyngitis) or
* nasal congestion significant enough to interfere with successful inoculation, or
* otitis media


* Born at less than 34 weeks gestation
* Born at less than 37 weeks gestation, and at the date of inoculation less than 1 year of age
* Maternal history of a positive HIV test
* Evidence of chronic disease
* Known or suspected infection or impairment of immunological functions
* Bone marrow/solid organ transplant recipient
* Major congenital malformations, including congenital cleft palate or cytogenetic abnormalities
* Suspected or documented developmental disorder, delay, or other developmental problem
* Cardiac abnormality requiring treatment
* Lung disease or reactive airway disease
* More than one episode of wheezing in the first year of life
* Wheezing episode or received bronchodilator therapy within the past 12 months
* Wheezing episode or received bronchodilator therapy after the age of 12 months
* Previous receipt of supplemental oxygen therapy in a home setting
* Previous receipt of an investigational RSV vaccine
* Previous receipt or planned administration of anti-RSV antibody product including ribavirin, RSV Ig, or RSV mAb
* Previous receipt of immunoglobulin or any antibody products within the past 6 months
* Previous receipt of any blood products within the past 6 months
* Previous anaphylactic reaction
* Previous vaccine-associated adverse reaction that was Grade 3 or above
* Known hypersensitivity to any study product component
* Member of a household that contains an infant who is less than 6 months of age at the date of inoculation through the 28th day after inoculation
* Member of a household that, at the date of inoculation through the 28th day after inoculation, contains an immunocompromised individual including but not limited to:

* a person who is HIV-infected
* a person who has cancer and has received chemotherapy within the 12 months prior to enrollment
* a person living with a solid organ or bone marrow transplant
* Attends a daycare facility that does not separate children by age and contains an infant less than 6 months of age at the date of inoculation through the 28th day after inoculation
* Receipt of any of the following prior to enrollment:

* any inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days prior, or
* any live vaccine, other than rotavirus vaccine, within the 28 days prior, or
* another investigational vaccine or investigational drug within 28 days prior, or
* salicylate (aspirin) or salicylate-containing products within the past 28 days
* Scheduled administration of any of the following after planned inoculation

* inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days after, or
* any live vaccine other than rotavirus in the 28 days after, or
* another investigational vaccine or investigational drug in the 56 days after
* Receipt of any of the following medications within 3 days of study enrollment:

* systemic antibacterial, antiviral, antifungal, anti-parasitic, or antituberculous agents, whether for treatment or prophylaxis, or
* intranasal medications, or
* other prescription medications except the permitted concomitant medications listed below:
* Permitted concomitant medications (prescription or non-prescription) include nutritional supplements, medications for gastroesophageal reflux, eye drops, and topical medications, including (but not limited to) cutaneous (topical) steroids, topical antibiotics, and topical antifungal agents.
* Any of the following events at the time of enrollment:

* fever (temporal or rectal temperature of greater than or equal to 100.4°F), or
* upper respiratory signs or symptoms (rhinorrhea, cough, or pharyngitis) or
* nasal congestion significant enough to interfere with successful inoculation, or
* otitis media


* Currently enrolled in another RSV study
Minimum Eligible Age

6 Months

Maximum Eligible Age

60 Months

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Ruth A. Karron, MD

Role: PRINCIPAL_INVESTIGATOR

Johns Hopkins Bloomberg School of Public Health (JHSPH)

Locations

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Center for Immunization Research, Johns Hopkins Bloomberg School of Public Health

Baltimore, Maryland, United States

Site Status

Countries

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United States

References

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Karron RA, Luongo C, Woods S, Oliva J, Collins PL, Buchholz UJ; RSVPed Team. Evaluation of the Live-Attenuated Intranasal Respiratory Syncytial Virus (RSV) Vaccine RSV/6120/DeltaNS2/1030s in RSV-Seronegative Young Children. J Infect Dis. 2024 Feb 14;229(2):346-354. doi: 10.1093/infdis/jiad281.

Reference Type RESULT
PMID: 37493269 (View on PubMed)

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form: RSV-Seronegative

View Document

Document Type: Informed Consent Form: RSV-Seropositive

View Document

Other Identifiers

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CIR 322

Identifier Type: -

Identifier Source: org_study_id