Trial Outcomes & Findings for Assessing a Risk Model for G6PD Deficiency (NCT NCT03337152)

NCT ID: NCT03337152

Last Updated: 2021-11-12

Results Overview

The change in haemoglobin from baseline on exposure to primaquine for P.vivax treatment over treatment course to hemoglobin level at day 28.

Recruitment status

TERMINATED

Study phase

PHASE4

Target enrollment

54 participants

Primary outcome timeframe

28 days after enrollment

Results posted on

2021-11-12

Participant Flow

6 participants were enrolled into the trial but had not been randomized at the time of the study halt. Those 6 participants were thus never assigned to a study group.

Participant milestones

Participant milestones
Measure
1A: Primaquine
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Overall Study
STARTED
24
24
Overall Study
COMPLETED
13
13
Overall Study
NOT COMPLETED
11
11

Reasons for withdrawal

Reasons for withdrawal
Measure
1A: Primaquine
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Overall Study
Physician Decision
1
0
Overall Study
Lost to Follow-up
1
3
Overall Study
Adverse Event
3
1
Overall Study
Stopped drug dosing due to study halt
6
7

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
1A: Primaquine
n=24 Participants
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=24 Participants
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Total
n=48 Participants
Total of all reporting groups
Region of Enrollment
Thailand
24 participants
n=24 Participants
24 participants
n=24 Participants
48 participants
n=48 Participants
G6PD Status
Heterozygous Female
5 Participants
n=24 Participants
4 Participants
n=24 Participants
9 Participants
n=48 Participants
Age, Continuous
33.5 years
n=24 Participants
38.5 years
n=24 Participants
36 years
n=48 Participants
Sex: Female, Male
Female
16 Participants
n=24 Participants
16 Participants
n=24 Participants
32 Participants
n=48 Participants
Sex: Female, Male
Male
8 Participants
n=24 Participants
8 Participants
n=24 Participants
16 Participants
n=48 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
G6PD Status
Homozygous Female
11 Participants
n=24 Participants
12 Participants
n=24 Participants
23 Participants
n=48 Participants
G6PD Status
Hemizygous Male
8 Participants
n=24 Participants
8 Participants
n=24 Participants
16 Participants
n=48 Participants
Hemoglobin
13.2 g/dL
n=24 Participants
12.65 g/dL
n=24 Participants
12.9 g/dL
n=48 Participants
G6PD Concentration
7.66 IU/g Hb
n=24 Participants
7.515 IU/g Hb
n=24 Participants
7.58 IU/g Hb
n=48 Participants

PRIMARY outcome

Timeframe: 28 days after enrollment

Population: Among those with available data at baseline and at least one timepoint after study treatment began.

The change in haemoglobin from baseline on exposure to primaquine for P.vivax treatment over treatment course to hemoglobin level at day 28.

Outcome measures

Outcome measures
Measure
1A: Primaquine
n=24 Participants
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=24 Participants
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Change in Haemoglobin
-1.1 g/dL
Interval -4.1 to 0.0
-1.05 g/dL
Interval -4.5 to 0.0

PRIMARY outcome

Timeframe: 28 days after enrollment

Population: Among those with available data at baseline and at least one timepoint after study treatment began.

The haemoglobin-related change in G6PD concentration, as determined by spectrometer, over treatment course. Change is determined from baseline to day 28

Outcome measures

Outcome measures
Measure
1A: Primaquine
n=24 Participants
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=24 Participants
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Change in G6PD Concentration
-1.23 IU/g Hb
Interval -2.99 to -0.34
-1.94 IU/g Hb
Interval -6.09 to -0.39

SECONDARY outcome

Timeframe: 28 days after enrollment

Population: Analysis not possible due to the study termination as the planned risk model could not be developed without sufficient data. Relevance of CYP2D6 results in the model thus unable to be determined.

relevance of Dextromethorphan assay results to risk of haemolysis models

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Days 1,2,3,5,7,9,11,14,17,21

Population: This association was dependent on developing a model which requires completion of the study for an appropriate sample size. The incompletion of the study resulted in it not being possible to generate this model and therefore assess the association of drug levels with hematological and G6PD activity levels

Association of chloroquine and primaquine drug levels at the time of sampling for haematological and G6PD profiles.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: 28 days after enrollment

Population: All randomized women heterozygous for G6PD.

frequency of serious adverse events in women heterozygous for G6PD

Outcome measures

Outcome measures
Measure
1A: Primaquine
n=5 Participants
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=4 Participants
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Serious Adverse Events
3 Participants
1 Participants

SECONDARY outcome

Timeframe: Days 1,2,3,5,7,9,11,14,17,21

Population: The relevance of reticulocyte count was dependent on developing a model which requires completion of the study for an appropriate sample size. The incompletion of the study resulted in it not being possible to generate this model and therefore assess the relevance of reticulocyte count on risk of hemolysis

relevance of reticulocyte count to risk of haemolysis models

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Days 1,2,3,5,7,9,11,14,17,21

Population: The relevance of urobilinogen tests of was dependent on developing a model which requires completion of the study for an appropriate sample size. The incompletion of the study resulted in it not being possible to generate this model and therefore assess the relevance of urobilinogen tests on hemolytic risk models

relevance of urobilinogen tests to risk of haemolysis models

Outcome measures

Outcome data not reported

Adverse Events

1A: Primaquine

Serious events: 3 serious events
Other events: 8 other events
Deaths: 0 deaths

1B: Chloroquine + Primaquine

Serious events: 1 serious events
Other events: 17 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
1A: Primaquine
n=24 participants at risk
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=24 participants at risk
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Blood and lymphatic system disorders
Hemolysis
12.5%
3/24 • Number of events 3 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment

Other adverse events

Other adverse events
Measure
1A: Primaquine
n=24 participants at risk
primaquine: Participants receive primaquine for 14 days at 0.5 mg/Kg.
1B: Chloroquine + Primaquine
n=24 participants at risk
chloroquine + primaquine: Participants will receive chloroquine for 3 days concomitant with primaquine for 14 days at 0.5 mg/Kg.
Blood and lymphatic system disorders
Anemia
12.5%
3/24 • Number of events 3 • 28 days after enrollment
0.00%
0/24 • 28 days after enrollment
Eye disorders
Blurred vision
4.2%
1/24 • Number of events 1 • 28 days after enrollment
25.0%
6/24 • Number of events 6 • 28 days after enrollment
Injury, poisoning and procedural complications
Wound
4.2%
1/24 • Number of events 1 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Respiratory, thoracic and mediastinal disorders
Common cold
8.3%
2/24 • Number of events 3 • 28 days after enrollment
8.3%
2/24 • Number of events 2 • 28 days after enrollment
Gastrointestinal disorders
Decreased appetite
8.3%
2/24 • Number of events 2 • 28 days after enrollment
20.8%
5/24 • Number of events 6 • 28 days after enrollment
General disorders
Difficulty sleeping
8.3%
2/24 • Number of events 2 • 28 days after enrollment
20.8%
5/24 • Number of events 5 • 28 days after enrollment
General disorders
Dizziness
4.2%
1/24 • Number of events 1 • 28 days after enrollment
45.8%
11/24 • Number of events 12 • 28 days after enrollment
General disorders
Fatigue
8.3%
2/24 • Number of events 2 • 28 days after enrollment
16.7%
4/24 • Number of events 4 • 28 days after enrollment
Gastrointestinal disorders
Gastritis
4.2%
1/24 • Number of events 1 • 28 days after enrollment
0.00%
0/24 • 28 days after enrollment
Renal and urinary disorders
Glucosuria
4.2%
1/24 • Number of events 1 • 28 days after enrollment
0.00%
0/24 • 28 days after enrollment
General disorders
Headache
16.7%
4/24 • Number of events 4 • 28 days after enrollment
12.5%
3/24 • Number of events 3 • 28 days after enrollment
Blood and lymphatic system disorders
Methemoglobinemia
4.2%
1/24 • Number of events 1 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Respiratory, thoracic and mediastinal disorders
Pharyngitis
4.2%
1/24 • Number of events 1 • 28 days after enrollment
0.00%
0/24 • 28 days after enrollment
Respiratory, thoracic and mediastinal disorders
Viral infection
4.2%
1/24 • Number of events 2 • 28 days after enrollment
0.00%
0/24 • 28 days after enrollment
Eye disorders
Conjunctivitis
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Psychiatric disorders
Depression
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Gastrointestinal disorders
Diarrhea
0.00%
0/24 • 28 days after enrollment
12.5%
3/24 • Number of events 3 • 28 days after enrollment
Renal and urinary disorders
Elevated liver function test
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Blood and lymphatic system disorders
Hematuria
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Blood and lymphatic system disorders
Hemolysis
0.00%
0/24 • 28 days after enrollment
8.3%
2/24 • Number of events 2 • 28 days after enrollment
General disorders
Pain
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Metabolism and nutrition disorders
Vitamin B1 deficiency
0.00%
0/24 • 28 days after enrollment
4.2%
1/24 • Number of events 1 • 28 days after enrollment
Gastrointestinal disorders
Nausea
0.00%
0/24 • 28 days after enrollment
8.3%
2/24 • Number of events 2 • 28 days after enrollment

Additional Information

Gonzalo Domingo

PATH

Phone: 206-285-3500

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place