Metabolomic Profile of Urine Samples From Neonates Fed With Breast Milk and an Infant Formula Enriched With Synbiotics

NCT ID: NCT03320837

Last Updated: 2023-11-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

108 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-08-01

Study Completion Date

2022-12-15

Brief Summary

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The aim of this trial is to verify - based on metabolomics and other anthropometric parameters - whether exclusive nutrition of infant formula enriched with synbiotics (probiotics B.lactis and prebiotics FOS), Long-Chain Polyunsaturated Fatty Acids (AA, DHA) and nucleotides, is comparable to breast milk and to mixed nutrition including breast milk together with the enriched infant formula.

Detailed Description

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Normal full-term infants aged 0-28 days, from singlet pregnancy, born by vaginal delivery or by caesarian section, with Apgar score \>7 in the 1st and 5th minute, not requiring resuscitation during delivery, and with a normal intrauterine growth, i.e. between 11th and 89th percentile (AGA- appropriate for gestational age). The mother's medical history should be free of any chronic diseases and allergies, while the gestation history should be free of any complications and of any pharmaceutical treatment administration prior to delivery.

Exclusion criteria: insufficient weight gain and/or semiology of possible allergy to cow milk, hospitalization in NICU (Neonatal Intensive Care Unit), treatment of breastfeeding mother having given birth recently or of the neonate with antibiotics after delivery.

Method: 36 Infants in exclusive breastfeeding, 36 infants feeding exclusively on infant formula with synbiotics and 36 infants following mixed nutrition with breast milk and infant formula with synbiotics, for a period of 3 months have been selected on randomized basis. For all these three groups, the anthropometric parameters should be measured (length, weight, head perimeter), since the first day of newborn's inclusion in the trial and at the age of 15 days, 2 and 3 months. Moreover, at the age of 15 days, 2 months, 3 months and on the infant's inclusion day in the trial, newborns-infants urine sample should be collected by the attending doctor using a ball of cotton inserted into the disposable diaper and with the aid of an aspiration syringe the urine sample (2-3 ml) will be transferred to a sterile vial and finally stored at -80 °C. Then frozen urine samples will be sent in batches to a contracted diagnostic center in order to examine the metabolic profile of the three diets described above (exclusive breastfeeding, infant formula with synbiotics and mixed nutrition with breast milk and infant formula with synbiotics), with the help of NMR and selectively liquid chromatography /mass spectrometry (LC/MS). This urine collection gives reliable results and can be used in infants without causing any discomfort or side-effect.

Conditions

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Infant Development

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Breastfeeding group

Infant are exclusively fed with breast milk

Breast milk

Intervention Type OTHER

Breast milk

Mixed feeding group

Infants are fed with mixed nutrition with breast milk and Rontamil Complete 1®

Infant formula

Intervention Type DIETARY_SUPPLEMENT

Breast milk substitute containing synbiotics

Breast milk

Intervention Type OTHER

Breast milk

Infant formula group

Infant are fed exclusively with Rontamil Complete 1®

Infant formula

Intervention Type DIETARY_SUPPLEMENT

Breast milk substitute containing synbiotics

Interventions

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Infant formula

Breast milk substitute containing synbiotics

Intervention Type DIETARY_SUPPLEMENT

Breast milk

Breast milk

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Normal full-term infants aged 0-28 days,
* From singlet pregnancy,
* Born by vaginal delivery or by caesarian section, with Apgar score \>7 in the 1st and 5th minute,
* Not requiring resuscitation during delivery, and with a normal intrauterine growth, i.e. between 11th and 89th percentile (AGA- appropriate for gestational age).

Exclusion Criteria

* Insufficient weight gain and/or semiology of possible allergy to cow milk,
* Hospitalization in NICU (Neonatal Intensive Care Unit),
* Treatment of breastfeeding mother having given birth recently or of the neonate with antibiotics after delivery.
Minimum Eligible Age

1 Day

Maximum Eligible Age

28 Days

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National and Kapodistrian University of Athens

OTHER

Sponsor Role collaborator

National Hellenic Research Foundation

OTHER

Sponsor Role collaborator

Rontis Hellas SA

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Nicoletta Iacovidou, PhD

Role: STUDY_DIRECTOR

National and Kapodistrian University of Athens

Locations

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Aretaieio Hospital

Athens, Attica, Greece

Site Status

Countries

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Greece

Other Identifiers

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metabolomicsurinesynbio3x36nmr

Identifier Type: -

Identifier Source: org_study_id