Contraceptive/HIV Affecting Risk in Adolescents

NCT ID: NCT03301480

Last Updated: 2019-02-07

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Total Enrollment

120 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-07-31

Study Completion Date

2018-09-17

Brief Summary

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This study will evaluate the impact of hormonal contraceptives on HIV risk associated with changes to the innate immunity in the female genital tract in a cross-sectional study. HIV risk will be evaluated by the capacity of cervical tissue to replicate HIV when challenged ex vivo and correlated to the number of CD4 T cells, DCs, and macrophages; the capacity of cervicovaginal fluid to inhibit HIV will be correlated to soluble mucosal mediators, and the vaginal microbiota. The lower genital tract samples will be collected from 120 adolescents aged 18-19 (40 using no hormonal contraception, 40 using ENG-I, 40 using LNG-IUS). For comparison, 90 adult women aged 25-45 will be equally distributed between the same groupings.

Detailed Description

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Conditions

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Contraception Hiv

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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No contraception/18-19 years old

No interventions assigned to this group

Use of ENG-I/18 - 19 years old

No interventions assigned to this group

LNG-IUS/18-19 years old

No interventions assigned to this group

No Contraception/ 25 - 45 years old

No interventions assigned to this group

Use of ENG-I/25 - 45 years old

No interventions assigned to this group

LNG-IUS/25-45 years old

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

1. Meets one of the following age groups (at the time of sample collection):

1. Adolescent aged 18-19
2. Adult aged 25-45
2. Meets one of the following contraception groups:

1. Regular menstrual cycles by participant history (if not using hormonal contraception)
2. Use of ENG-I (i.e. Implanon/Nexplanon) for at least 30 days by participant history
3. Use of LNG-IUS (Mirena or Skyla) for at least 30 days by participant history
3. HIV uninfected Note: HIV testing will be done as part of screening. However, if a woman participated in a recent research study and has an HIV test result available from that study, this will suffice provided the date of the test is within 6 months of the study visit.
4. Willing to undergo pelvic exam and collection of cervical biopsies
5. Willing to provided written informed consent
6. Agree to be sexually abstinent for 48 hours prior to the cervical biopsies and for one week after the biopsies.

Exclusion Criteria

1. Use of any other hormonal contraception other than ENG-I or LNG-IUS
2. Use of a diaphragm or spermicide for contraception
3. Pregnant or pregnancy within 90 days
4. Currently breastfeeding
5. Currently menstruating/bleeding (at time of specimen collection) Note: participant can return after cessation of bleeding for specimen collection
6. Past participation in more than one study involving cervical biopsies per participant report
7. Cervical biopsies within one month of specimen collection visit
8. Abnormal vaginal discharge or other genital tract symptoms at the time of specimen collection
9. Pelvic findings on the day of specimen collection consistent with cervicitis (i.e. MPC, erythema, edema) or anatomy making cervical biopsies difficult
10. Menopausal
11. Hysterectomy
12. History of malignancy of the genital tract (including cervix, uterus, vagina, and vulva)
13. History of immunosuppression (including HIV infection, diabetes, and chronic steroid use)
14. Known history of a platelet/bleeding/clotting disorder
15. Use of a systemic or vaginal antimicrobial agent within 7 days of specimen collection
16. Use of any vaginal product (i.e. douching, spermicide, lubricant) or device within 7 days of specimen collection Note: tampon use is acceptable
17. Any other condition that in the opinion of the Study Investigator would preclude provision of informed consent or make study participation unsafe, complicate the interpretation of study outcome data, or otherwise interfere with achieving the study objectives.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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National Institutes of Health (NIH)

NIH

Sponsor Role collaborator

University of Pittsburgh

OTHER

Sponsor Role lead

Responsible Party

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Katherine Bunge

Assistant Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Katherine Bunge, MD

Role: PRINCIPAL_INVESTIGATOR

University of Pittsburgh

Locations

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Magee-Womens Hospital of UPMC

Pittsburgh, Pennsylvania, United States

Site Status

Countries

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United States

Other Identifiers

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PRO17050002

Identifier Type: -

Identifier Source: org_study_id

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