Correlation Between Tissue and Plasmatic EGFR in CBNPC With EGFR Mutation or Predictive Factor of EGFR Mutation

NCT ID: NCT03265496

Last Updated: 2021-10-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

NA

Total Enrollment

21 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-07-07

Study Completion Date

2021-04-13

Brief Summary

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Condorde main objective is to evaluate the correlation between EGFR mutational status determined after tumor biopsy and EGFR mutational status analyzed on ctDNA after liquid biopsy. EGFR status will be assessed by real time PCR (rtPCR), digital PCR (dPCR) and Next Generation Sequencing (NGS) in patients with chemotherapy naive lung carcinoma.

Detailed Description

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Newest therapeutic breakthrough are often based on molecular analysis of tumoral tissue before treatment initiation or after emergence of resistance. Tumoral tissue is commonly obtained by biopsy. However, tumor biopsy is an invasive, scarcely repeatable and costly technics. Moreover, tumor samples, obtained by biopsy, doesn't represent tumor heterogeneity and cannot inform about tumor evolution over time.

Recent improvement have been done in detection and characterization of blood circulating tumoral DNA (ctDNA). ctDNA reach regularly the blood stream after tumoral cell apoptosis or necrosis and could be extract and sequenced by some molecular biology technics such as real time PCR (rtPCR), digital PCR (dPCR) or next generation sequencing (NGS). Interesting, Several studies demonstrate that some genomic alterations of solid cancer can be characterized after sequencing of ctDNA. Other experiments pointed that ctDNA level could be linked to tumor stage and patient prognostic.

These progress lead to the development of a new non invasive method for extraction of ctDNA called liquid biopsy (LB). LB could be useful for monitor tumoral genotype, assess tumor response to treatment and detect residual tumor cells after curative treatment. Moreover LB could be an essential method for study of tumor cells molecular alterations mechanisms during targeted cancer therapies, when clinical resistance occurs.

Non-small cell lung cancer (NSCLC) is the most frequently diagnosticated type of lung cancer. Regular first line chemotherapy is based on the use of platinum salts. However, some mutations in the EGFR gene could add sensitivity of NSCLC to tyrosine kinase inhibitors such as gefitinib, erlotinib or afatinib. Consequently, the search for molecular mutations in genome of NSCLC cells is of prior interest for patients with clinically advanced NSCLC.

Recently, some studies demonstrate that mutational EGFR status of NSCLC was sharply correlated between tumoral tissue, obtained by classical biopsy, and ctDNA, collected by liquid biopsy.

These results provide promising data encouraging the use of LB for study of NSCLC ctDNA. However some experimentations are needed to ensure these data.

For that reason, CONCORDE clinical trial will evaluate the correlation between EGFR mutational status determined after tumor biopsy and EGFR mutational status analyzed after liquid biopsy. EGFR status will be assessed by real time PCR (rtPCR), digital PCR (dPCR) and Next Generation Sequencing (NGS) in patients with chemotherapy naive lung carcinoma.

Conditions

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Nsclc

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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study procedure

Clinical exam. Liquid biopsy. Diagnostic exam (biopsy and imagery). 1st line treatment. tumor evaluation. Biopsy

Group Type EXPERIMENTAL

Clinical exam

Intervention Type PROCEDURE

Clinical exam is performed before treatment start

Liquid biopsy

Intervention Type PROCEDURE

Liquid biopsy will be performed at baseline and every 3 cycles of chemotherapy until progression disease

Diagnostic exam

Intervention Type PROCEDURE

Diagnostic exam (biopsy and imagery exam) will be performed at baseline if not previously done or incompletely done.

1st line treatment

Intervention Type DRUG

1st line chemotherapy (chemotherapy or EGFR targeted therapy) will be performed until progression disease.

tumor evaluation

Intervention Type PROCEDURE

Tumor evaluation will be performed every 3 cycles of chemotherapy

Biopsy

Intervention Type BIOLOGICAL

Biopsy will be performed at the end of study, after progression disease, if a mutation of EGFR is detected in tumor DNA.

Interventions

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Clinical exam

Clinical exam is performed before treatment start

Intervention Type PROCEDURE

Liquid biopsy

Liquid biopsy will be performed at baseline and every 3 cycles of chemotherapy until progression disease

Intervention Type PROCEDURE

Diagnostic exam

Diagnostic exam (biopsy and imagery exam) will be performed at baseline if not previously done or incompletely done.

Intervention Type PROCEDURE

1st line treatment

1st line chemotherapy (chemotherapy or EGFR targeted therapy) will be performed until progression disease.

Intervention Type DRUG

tumor evaluation

Tumor evaluation will be performed every 3 cycles of chemotherapy

Intervention Type PROCEDURE

Biopsy

Biopsy will be performed at the end of study, after progression disease, if a mutation of EGFR is detected in tumor DNA.

Intervention Type BIOLOGICAL

Other Intervention Names

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1st line chemotherapy

Eligibility Criteria

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Inclusion Criteria

* At least 18 years
* Metastatic lung carcinoma
* With: - EGFR gene mutation
* Or at least 2 predictive factors of addictive mutation (Women, non smocking or cessation \> 3 years, Asiatic, lung adenocarcinoma)
* Eligible for 1st line treatment
* Performance status ≤ 3
* Available tumor sample or tumor reachable for biopsy
* With informed and signed consent
* Affiliation to the National Social Security System

Exclusion Criteria

* Previous radiotherapy treatment in months preceding initials samples
* Pregnant or breastfeeding women
* Patient not able to give consent or unwilling to provide consent
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Lille

OTHER

Sponsor Role collaborator

Centre Oscar Lambret

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Kotecki Nuria, MD

Role: PRINCIPAL_INVESTIGATOR

Centre Oscar Lambret

Cortot Alexis, Pr

Role: PRINCIPAL_INVESTIGATOR

CHRU LILLE

Locations

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Centre Oscar Lambret

Lille, , France

Site Status

CHRU Lille

Lille, , France

Site Status

Countries

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France

Other Identifiers

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CONCORDE-1510

Identifier Type: -

Identifier Source: org_study_id