Trial Outcomes & Findings for Absorption and Elimination of Radiolabeled Daprodustat (NCT NCT03239522)

NCT ID: NCT03239522

Last Updated: 2019-12-03

Results Overview

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Pharmacokinetic Population comprised of all participants in the Safety Population who had at least 1 non-missing pharmacokinetic assessment (Non-quantifiable \[NQ\] values were considered as non-missing values).

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

6 participants

Primary outcome timeframe

Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Results posted on

2019-12-03

Participant Flow

This study evaluated the excretion balance of daprodustat(GSK1278863) using radiolabeled 14 Carbon \[14C\]-drug substance administered orally, and as an intravenous (IV) infusion, administered as a micro tracer dose (concomitant with an oral, non-radiolabeled dose) in healthy male participants.

Participants were enrolled at a single center in United Kingdom. A total of 6 participants were enrolled in the study. All the enrolled participants were randomized to receive study treatment.

Participant milestones

Participant milestones
Measure
All Participants Receiving GSK1278863
Participants received a single 6 milligram (mg) oral dose of GSK1278863 on Day 1 of treatment period 1, after an overnight fast of at least 8 hours. After approximately 1 hour, participants received 50 microgram (µg) \[14C\]-GSK1278863 by IV infusion over 1 hour. It was followed by a washout period of 7 days. On Day 1 of treatment period 2, each participant received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours.
Treatment Period 1 (Up to 7 Days)
STARTED
6
Treatment Period 1 (Up to 7 Days)
COMPLETED
6
Treatment Period 1 (Up to 7 Days)
NOT COMPLETED
0
Washout Period (Up to 7 Days)
STARTED
6
Washout Period (Up to 7 Days)
COMPLETED
6
Washout Period (Up to 7 Days)
NOT COMPLETED
0
Treatment Period 2 (Up to 15 Days)
STARTED
6
Treatment Period 2 (Up to 15 Days)
COMPLETED
6
Treatment Period 2 (Up to 15 Days)
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Absorption and Elimination of Radiolabeled Daprodustat

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Participants Receiving GSK1278863
n=6 Participants
Participants received a single 6 milligram (mg) oral dose of GSK1278863 on Day 1 of treatment period 1, after an overnight fast of at least 8 hours. After approximately 1 hour, participants received 50 microgram (µg) \[14C\]-GSK1278863 by IV infusion over 1 hour. It was followed by a washout period of 7 days. On Day 1 of treatment period 2, each participant received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours.
Age, Continuous
40.7 Years
STANDARD_DEVIATION 8.57 • n=5 Participants
Sex: Female, Male
Female
0 Participants
n=5 Participants
Sex: Female, Male
Male
6 Participants
n=5 Participants
Race/Ethnicity, Customized
WHITE - ARABIC/NORTH AFRICAN HERITAGE
1 Participants
n=5 Participants
Race/Ethnicity, Customized
WHITE - WHITE/CAUCASIAN/EUROPEAN HERITAGE
5 Participants
n=5 Participants

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Pharmacokinetic Population comprised of all participants in the Safety Population who had at least 1 non-missing pharmacokinetic assessment (Non-quantifiable \[NQ\] values were considered as non-missing values).

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=5 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Area Under the Concentration-time Curve From Time Zero (Pre-dose) Extrapolated to Infinite Time (AUC [0-Inf]) of Total Drug-related Material (Radioactivity) in Plasma Following Administration of GSK1278863
6.6864 Hour*nanogram equivalent per milliliter
Geometric Coefficient of Variation 19.8
2278.8914 Hour*nanogram equivalent per milliliter
Geometric Coefficient of Variation 18.2

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation). Data were not analyzed for radiolabeled oral dose GSK1278863 as there were not enough data points captured for a terminal slope required to calculate AUC (0-inf).

Blood samples were planned to be collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error. Data were not analyzed for radiolabeled oral dose of GSK1278863 as there were not enough data points captured for a terminal slope required to calculate AUC (0-inf). The blood assay could not detect radiation levels at the time points blood was drawn to go into these calculations.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC From Time Zero (Pre-dose) to Last Time of Quantifiable Concentration Within a Participant Across All Treatments (AUC [0-t]) of Total Drug-related Material (Radioactivity) in Plasma Following Administration of GSK1278863
6.5252 Hour*nanogram equivalent per milliliter
Geometric Coefficient of Variation 18.9
2206.7099 Hour*nanogram equivalent per milliliter
Geometric Coefficient of Variation 18.5

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation).

Blood samples were collected from participants at indicated time points after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC (0-t) of Total Drug-related Material (Radioactivity) in Blood Following Administration of GSK1278863
824.6102 Hour*nanogram equivalent per milliliter
Geometric Coefficient of Variation 17.2

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Maximum Observed Plasma Concentration (Cmax) of Total Drug-related Material (Radioactivity) in Plasma Following Administration of GSK1278863
3.0114 Nanogram equivalent per milliliter
Geometric Coefficient of Variation 15.8
623.9059 Nanogram equivalent per milliliter
Geometric Coefficient of Variation 34.8

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation).

Blood samples were collected from participants at indicated time points after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Cmax of Total Drug-related Material (Radioactivity) in Blood Following Administration of GSK1278863
289.9998 Nanogram equivalent per milliliter
Geometric Coefficient of Variation 30.7

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Time of Occurrence of Cmax (Tmax) of Total Drug-related Material (Radioactivity) in Plasma Following Administration of GSK1278863
0.9833 Hour
Interval 0.983 to 1.25
0.7583 Hour
Interval 0.5 to 3.0

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation).

Blood samples were collected from participants at indicated time points after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Tmax of Total Drug-related Material (Radioactivity) in Blood Following Administration of GSK1278863
1.0000 Hour
Interval 1.0 to 3.0

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=5 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Apparent Terminal Phase Half-life (t1/2) of Total Drug-related Material (Radioactivity) in Plasma Following Administration of GSK1278863
6.7699 Hour
Geometric Coefficient of Variation 106.4
61.8251 Hour
Geometric Coefficient of Variation 15.1

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation). Data were not analyzed for radiolabeled oral dose GSK1278863 as there were not enough data points captured for a terminal slope required to calculate t1/2.

Blood samples were planned to be collected from participants at indicated time points in each of the treatment period 1 and 2, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error. Data were not analyzed for radiolabeled oral dose of GSK1278863 as there were not enough data points captured for a terminal slope required to calculate t1/2. The blood assay could not detect radiation levels at the time points blood was drawn to go into these calculations.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points in treatment period 1, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=5 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Volume of Distribution at Steady State (Vss) of Total Drug-related Material (Radioactivity) in Plasma Following IV Dose of GSK1278863
32.2355 Liters
Geometric Coefficient of Variation 59.1

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation).

Blood samples were planned to be collected from participants at indicated time points in treatment period 1, after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points in treatment period 1 after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=5 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Total Systemic Clearance (CL) of Total Drug-related Material (Radioactivity) in Plasma Following IV Dose of GSK1278863
7.4779 Liters per hour
Geometric Coefficient of Variation 19.8

PRIMARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Data were not collected for radiolabeled IV dose of GSK1278863 because of an error (deviation).

Blood samples were planned to be collected from participants at indicated time points in treatment period 1 after administration of study treatment to investigate the pharmacokinetics of GSK1278863 in blood. Data were not collected for blood total radioactivity concentration following administration of radiolabeled IV dose of GSK1278863 because of an error (deviation). The deviation is due to a processing error: labels for whole blood draws, and aliquots for shipment were not generated in error.

Outcome measures

Outcome data not reported

PRIMARY outcome

Timeframe: Pre-dose and then over 24 hours collection periods as follows: 0-24, 24-48, 48-72, 72-96, 96-120,120-144 and 144-168 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Urine samples were collected at the indicated time points to determine the rate and extent of excretion of total radioactivity in urine. All participants were asked to void their bladders before study treatment administration.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
Pre-dose; n=6
0.000 Percent dose excreted
Standard Deviation 0.0000
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
0-24 hours; n=6
20.450 Percent dose excreted
Standard Deviation 2.7441
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
24-48 hours; n=6
20.838 Percent dose excreted
Standard Deviation 2.8280
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
48-72 hours; n=6
21.003 Percent dose excreted
Standard Deviation 2.8723
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
72-96 hours; n=6
21.065 Percent dose excreted
Standard Deviation 2.8744
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
96-120 hours; n=6
21.065 Percent dose excreted
Standard Deviation 2.8744
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
120-144 hours; n=6
21.065 Percent dose excreted
Standard Deviation 2.8744
Percentage of the Total Radioactive Dose Excreted in Urine Over Time Following a Single, Oral Dose of [14C]-GSK1278863
144-168 hours; n=5
21.434 Percent dose excreted
Standard Deviation 3.0507

PRIMARY outcome

Timeframe: Pre-dose and then over 24 hour collection periods as follows: 0-24, 24-48, 48-72, 72-96, 96-120, 120-144 and 144-168 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Fecal samples were collected at the indicated time points to determine the rate and extent of excretion of total radioactivity in feces.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
Pre-dose; n=6
0.000 Percent dose excreted
Standard Deviation 0.0000
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
0-24 hours; n=5
0.096 Percent dose excreted
Standard Deviation 0.1292
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
24-48 hours; n=5
22.298 Percent dose excreted
Standard Deviation 31.0164
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
48-72 hours; n=6
49.988 Percent dose excreted
Standard Deviation 25.3857
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
72-96 hours; n=6
68.312 Percent dose excreted
Standard Deviation 6.0872
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
96-120 hours; n=5
73.450 Percent dose excreted
Standard Deviation 3.1046
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
120-144 hours; n=5
74.698 Percent dose excreted
Standard Deviation 2.1027
Percentage of the Total Radioactive Dose Excreted in Feces Over Time Following a Single, Oral Dose of [14C]-GSK1278863
144-168 hours; n=4
73.553 Percent dose excreted
Standard Deviation 3.6611

PRIMARY outcome

Timeframe: Pre-dose and then over 24 hour collection periods as follows: 0-24, 24-48, 48-72, 72-96, 96-120, 120-144 and 144-168 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Urine and fecal samples were collected at the indicated time points to determine the rate and extent of cumulative excretion of total radioactivity in urine and feces.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
Pre-dose; n=6
0.000 Percent dose excreted
Standard Deviation 0.0000
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
0-24 hours; n=6
20.530 Percent dose excreted
Standard Deviation 2.7223
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
24-48 hours; n=6
39.433 Percent dose excreted
Standard Deviation 28.2968
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
48-72 hours; n=6
70.992 Percent dose excreted
Standard Deviation 25.9980
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
72-96 hours; n=6
89.377 Percent dose excreted
Standard Deviation 6.3351
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
96-120 hours; n=6
94.177 Percent dose excreted
Standard Deviation 2.1428
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
120-144 hours; n=6
94.705 Percent dose excreted
Standard Deviation 2.0982
Percentage of the Total Radioactive Dose Excreted in Urine and Feces Determined as Total Excretion Over Time
144-168 hours; n=5
94.582 Percent dose excreted
Standard Deviation 2.3066

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points, after administration of IV dose, both radiolabeled and non-radiolabeled oral doses of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=3 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC (0-Inf) of GSK1278863 in Plasma Following Administration IV and Both Oral Doses
199.2552 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.9
2.6509 Hour*nanogram per milliliter
Geometric Coefficient of Variation 27.4
940.2011 Hour*nanogram per milliliter
Geometric Coefficient of Variation 28.6

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points, after administration of IV dose, both radiolabeled and non-radiolabeled oral doses of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC(0-t) of GSK1278863 in Plasma Following Administration of IV and Both Oral Doses
198.1658 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.8
2.6368 Hour*nanogram per milliliter
Geometric Coefficient of Variation 20.4
938.5541 Hour*nanogram per milliliter
Geometric Coefficient of Variation 28.7

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points, after administration of IV dose, both radiolabeled and non-radiolabeled oral doses of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Cmax of GSK1278863 in Plasma Following Administration of IV and Both Oral Doses
96.1190 Nanogram per milliliter
Geometric Coefficient of Variation 17.2
2.0058 Nanogram per milliliter
Geometric Coefficient of Variation 25.5
544.7022 Nanogram per milliliter
Geometric Coefficient of Variation 34.1

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population

Plasma samples were collected from participants at indicated time points, after administration of IV dose, both radiolabeled and non-radiolabeled oral doses of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Tmax of GSK1278863 in Plasma Following Administration of IV and Both Oral Doses
2.1167 Hour
Interval 1.417 to 4.0
0.983 Hour
Interval 0.983 to 0.983
0.5000 Hour
Interval 0.5 to 0.517

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points, after administration of IV dose, both radiolabeled and non-radiolabeled oral doses of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=3 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
T1/2 of GSK1278863 in Plasma Following Administration of IV and Both Oral Doses
1.8786 Hour
Geometric Coefficient of Variation 21.6
2.0653 Hour
Geometric Coefficient of Variation 6.6
2.5037 Hour
Geometric Coefficient of Variation 30.2

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were collected from participants at indicated time points, for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863.Pharmacokinetic analysis was conducted using standard non-compartmental methods. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles). Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2391220; n=6,0,5
33.7699 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.1
139.7713 Hour*nanogram per milliliter
Geometric Coefficient of Variation 19.0
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2487818; n=6,0,6
21.7209 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.3
92.0554 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.7
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506102; n=6,0,6
8.6207 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.5
33.1332 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.6
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506104; n=6,0,5
34.1180 Hour*nanogram per milliliter
Geometric Coefficient of Variation 11.9
140.3785 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.7
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531398; n=6,0,6
15.0285 Hour*nanogram per milliliter
Geometric Coefficient of Variation 13.1
61.7760 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.4
AUC (0-Inf) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531401; n=6,0,6
34.4699 Hour*nanogram per milliliter
Geometric Coefficient of Variation 21.9
124.1320 Hour*nanogram per milliliter
Geometric Coefficient of Variation 25.5

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were collected from participants at indicated time points, for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2391220
32.2574 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.0
142.2907 Hour*nanogram per milliliter
Geometric Coefficient of Variation 18.9
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2487818
21.2933 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.8
90.9953 Hour*nanogram per milliliter
Geometric Coefficient of Variation 16.7
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506102
8.0798 Hour*nanogram per milliliter
Geometric Coefficient of Variation 14.1
31.8897 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.9
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506104
32.3120 Hour*nanogram per milliliter
Geometric Coefficient of Variation 11.9
142.3372 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.6
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531398
14.4743 Hour*nanogram per milliliter
Geometric Coefficient of Variation 13.4
60.2615 Hour*nanogram per milliliter
Geometric Coefficient of Variation 15.5
AUC(0-t) of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531401
31.7836 Hour*nanogram per milliliter
Geometric Coefficient of Variation 18.8
122.7102 Hour*nanogram per milliliter
Geometric Coefficient of Variation 25.8

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population.Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were collected from participants at indicated time points, for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2391220
7.5219 Nanogram per milliliter
Geometric Coefficient of Variation 14.4
31.5278 Nanogram per milliliter
Geometric Coefficient of Variation 30.4
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2487818
6.0391 Nanogram per milliliter
Geometric Coefficient of Variation 19.1
25.4542 Nanogram per milliliter
Geometric Coefficient of Variation 33.0
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506102
1.8233 Nanogram per milliliter
Geometric Coefficient of Variation 16.6
6.8139 Nanogram per milliliter
Geometric Coefficient of Variation 25.7
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506104
7.3607 Nanogram per milliliter
Geometric Coefficient of Variation 13.5
30.4258 Nanogram per milliliter
Geometric Coefficient of Variation 27.6
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531398
3.5279 Nanogram per milliliter
Geometric Coefficient of Variation 12.7
13.5590 Nanogram per milliliter
Geometric Coefficient of Variation 25.8
Cmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531401
6.4247 Nanogram per milliliter
Geometric Coefficient of Variation 23.6
21.9493 Nanogram per milliliter
Geometric Coefficient of Variation 36.8

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were collected from participants at indicated time points, for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2391220
3.5000 Hour
Interval 2.983 to 6.0
2.0000 Hour
Interval 1.5 to 4.0
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2487818
3.5000 Hour
Interval 1.983 to 6.0
2.0000 Hour
Interval 1.5 to 4.0
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506102
3.5000 Hour
Interval 2.983 to 6.0
2.0000 Hour
Interval 2.0 to 4.0
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506104
4.0000 Hour
Interval 2.983 to 6.0
2.0000 Hour
Interval 2.0 to 4.0
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531398
3.5000 Hour
Interval 2.983 to 6.0
2.0000 Hour
Interval 2.0 to 4.0
Tmax of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531401
4.0000 Hour
Interval 3.0 to 6.0
2.0000 Hour
Interval 2.0 to 4.0

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1; Pre-dose, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 2

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were collected from participants at indicated time points, for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles). Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2391220; n=6,0, 5
1.9805 Hour
Geometric Coefficient of Variation 7.9
3.1506 Hour
Geometric Coefficient of Variation 26.1
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2487818; n=6,0, 6
1.5744 Hour
Geometric Coefficient of Variation 12.5
1.8644 Hour
Geometric Coefficient of Variation 10.7
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506102; n=6,0, 6
2.0037 Hour
Geometric Coefficient of Variation 15.9
2.2738 Hour
Geometric Coefficient of Variation 5.1
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2506104; n=6,0, 5
2.0849 Hour
Geometric Coefficient of Variation 8.0
3.3870 Hour
Geometric Coefficient of Variation 10.9
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531398; n=6,0, 6
1.7006 Hour
Geometric Coefficient of Variation 14.7
1.9767 Hour
Geometric Coefficient of Variation 3.8
T1/2 of GSK1278863 Metabolites in Plasma Following Administration of IV and Both Oral Doses
GSK2531401; n=6,0, 6
2.3217 Hour
Geometric Coefficient of Variation 12.0
2.9197 Hour
Geometric Coefficient of Variation 9.9

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points, after administration of radiolabeled IV dose of GSK1278863 to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=3 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Vss of GSK1278863 in Plasma Following IV Dose Administration
14.3387 Liter
Geometric Coefficient of Variation 23.0

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were planned to be collected from participants at indicated time points, after administration of radiolabeled IV dose of GSK1278863 for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

Plasma samples were collected from participants at indicated time points, after administration of radiolabeled IV dose of GSK1278863 to investigate the pharmacokinetics of GSK1278863 in plasma. Pharmacokinetic analysis was conducted using standard non-compartmental methods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=3 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
CL of GSK1278863 in Plasma Following IV Dose Administration
18.8612 Liter per hour
Geometric Coefficient of Variation 27.4

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Plasma samples were planned to be collected from participants at indicated time points, after administration of radiolabeled IV dose of GSK1278863 for metabolite profiling. GSK2391220, GSK2506104, GSK2487818, GSK2506102, GSK2531398 and GSK2531401 were metabolites of GSK1278863. Pharmacokinetic analysis was conducted using standard non-compartmental methods. Data were not collected for GSK1278863 metabolites following IV dose as analysis of pharmacokinetic parameters of only parent compound following IV dose was of interest to calculate the bioavailability and not the metabolites.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Pre-dose, 0.5, 1, 1.25, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120 and 144 hours post-dose in treatment period 1

Population: Pharmacokinetic Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Absolute bioavailability is the amount of drug from a formulation that reaches the systemic circulation relative to an IV dose, computed as ratio of AUC(oral)/Dose(oral) with AUC(IV)/Dose(IV). Plasma samples were collected from participants at indicated time points. Absolute bioavailability from the oral tablet and IV doses administered in treatment period 1 was analyzed using AUC(0-inf) and AUC(0-t) pharmacokinetic parameters.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Absolute Bioavailability of GSK1278863 Following Oral Dosing
AUC (0-inf); n=3
0.6575 Ratio of dose normalized AUC
Geometric Coefficient of Variation 11.8
Absolute Bioavailability of GSK1278863 Following Oral Dosing
AUC (0-t); n=6
0.6263 Ratio of dose normalized AUC
Geometric Coefficient of Variation 10.7

SECONDARY outcome

Timeframe: 0-8 hours, 10-12 hours in period 2

Population: Pharmacokinetic Population

Blood samples were collected in treatment period 2 to measure total radioactivity and to characterize the metabolite profiling of GSK1278863 following a single oral dose of \[14C\]-GSK1278863 at 25 mg. Potential metabolites were M3 (GSK2506104), M2 (GSK2391220) and M33 combined, M4 (GSK2487818), M6 (GSK2531398), M13 (GSK2531401), M5 (GSK2506102) and M14 combined. 2 pooled plasma samples were prepared. Aliquots of plasma samples collected between 0 to 8 hours post-dose from each participant were pooled in proportion to the time intervals. An equal amount of the individual pools from each participant was then pooled to create 1 plasma sample that was representative of the mean area under curve over the range of 0-8 hour. The second pool (10-12 hours pool) was obtained by mixing equal volume of the plasma samples at 10 and 12 hour across all participants. Percent radioactivity recovered for each metabolite in plasma following a single oral dose of \[14C\]-GSK1278863 at 25 mg is presented.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M2 (GSK2391220) and M33 combined, 0-8 hours
10.4 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M2 (GSK2391220) and M33 combined, 10-12 hours
14.4 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M3 (GSK2506104), 0-8 hours
7.6 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M3 (GSK2506104), 10-12 hours
11.8 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M4 (GSK2487818), 0-8 hours
5.7 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M4 (GSK2487818), 10-12 hours
NA Percent radioactivity
Data is not available since concentration of metabolite was not detected in the sample.
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M6 (GSK2531398), 0-8 hours
3.6 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M6 (GSK2531398), 10-12 hours
NA Percent radioactivity
Data is not available as all concentration values were less than the lower limit of quantification.
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M13 (GSK2531401), 0-8 hours
8.3 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M13 (GSK2531401), 10-12 hours
16.1 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M5 (GSK2506102) and M14 combined, 0-8 hours
4.5 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Plasma Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M5 (GSK2506102) and M14 combined, 10-12 hours
NA Percent radioactivity
Data is not available as all concentration values were less than the lower limit of quantification.

SECONDARY outcome

Timeframe: 0-24 hours in period 2

Population: Pharmacokinetic Population

Urine samples were collected in treatment period 2 to measure total radioactivity and to characterize the metabolite profiling of GSK1278863 following a single oral dose of \[14C\]-GSK1278863 at 25 mg. Potential metabolites were M3 (GSK2506104), M2 (GSK2391220) and M33 combined, M4 (GSK2487818), M6 (GSK2531398), M13 (GSK2531401), M5 (GSK2506102) and M14 combined. One pooled urine sample was prepared by urine collected from 0 to 24 hours post dose from all participants. Percent radioactivity recovered for each metabolite in urine following a single oral dose of \[14C\]-GSK1278863 at 25 mg is presented.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M2 (GSK2391220) and M33 combined
15.8 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M3 (GSK2506104)
16.0 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M4 (GSK2487818)
7.9 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M6 (GSK2531398)
5.8 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M13 (GSK2531401)
16.5 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Urine Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M5 (GSK2506102) and M14 combined
10.4 Percent radioactivity

SECONDARY outcome

Timeframe: 0-120 hours in period 2

Population: Pharmacokinetic Population

Feces samples were collected in treatment period 2 to measure total radioactivity and to characterize the metabolite profiling of GSK1278863 following a single oral dose of \[14C\]-GSK1278863 at 25 mg. Potential metabolites were M3 (GSK2506104), M2 (GSK2391220), M4 (GSK2487818), M6 (GSK2531398), M13 (GSK2531401), M5 (GSK2506102) and M14 combined. One pooled feces sample was prepared by samples collected from 0 to 120 hours post dose from all participants. Percent radioactivity recovered for each metabolite in feces following a single oral dose of \[14C\]-GSK1278863 at 25 mg is presented.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M2 (GSK2391220)
19.8 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M3 (GSK2506104)
14.1 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M4 (GSK2487818)
17.0 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M6 (GSK2531398)
6.2 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M13 (GSK2531401)
4.9 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Feces Following a Single Oral Dose of [14C]-GSK1278863 at 25 mg
M5 (GSK2506102) and M14 combined
7.7 Percent radioactivity

SECONDARY outcome

Timeframe: 3 hours post-oral dose in period 1

Population: Pharmacokinetic Population. Only those participants with data available at specified time point were analyzed.

The bile string was swallowed by participants prior to oral dose and was removed 3 hours after the oral dose (1 hour after the end of the IV infusion) in treatment period-1. Duodenal bile string extracts were pooled to create a single pool sample to measure total radioactivity and to characterize the metabolite profiling of GSK1278863 following a single dose of \[14C\]-GSK1278863 50 µg IV infusion. Potential metabolites were M3 (GSK2506104), M2 (GSK2391220), M4 (GSK2487818), M6 (GSK2531398), M13 (GSK2531401), M5 (GSK2506102). Mean percent radioactivity recovered for each metabolite in bile following a single dose of \[14C\]-GSK1278863 50 µg IV infusion is presented.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=3 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M2 (GSK2391220)
12.3 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M3 (GSK2506104)
20.0 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M4 (GSK2487818)
15.5 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M6 (GSK2531398)
6.3 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M13 (GSK2531401)
2.4 Percent radioactivity
Percent Radioactivity Recovered for Each Metabolite in Duodenal Bile Following a Single Dose of [14C]-GSK1278863 50 µg IV Infusion
M5
11.1 Percent radioactivity

SECONDARY outcome

Timeframe: Up to 43 days

Population: Safety Population

An AE is any untoward medical occurrence in a clinical study participant, temporally associated with the use of a study treatment, whether or not considered related to the study treatment. A SAE is defined as any untoward medical occurrence that, at any dose which results in death, is life- threatening, requires inpatient hospitalization or prolongation of existing hospitalization, results in persistent disability/incapacity, is a congenital anomaly/birth defect, associated with liver injury and impaired liver function or any other situations as per Medical or scientific judgment. Safety Population comprised of all participants who take at least 1 dose of study treatment. Participants were analyzed according to the treatment they actually received.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With Adverse Events (AEs) and Serious AEs (SAEs)
Any AE
2 Participants
2 Participants
Number of Participants With Adverse Events (AEs) and Serious AEs (SAEs)
Any SAE
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Up to 43 days

Population: Safety Population

An AE is any untoward medical occurrence in a clinical study participant, temporally associated with the use of a study treatment, whether or not considered related to the study treatment. Severity was categorized as mild, moderate and severe. The number of participants with AEs at any type of severity (mild, moderate and severe) has been presented.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With AEs at a Particular Severity
Moderate
1 Participants
0 Participants
Number of Participants With AEs at a Particular Severity
Mild
2 Participants
2 Participants
Number of Participants With AEs at a Particular Severity
Severe
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Up to 43 days

Population: Safety Population

Blood samples were collected from participants at indicated time points for the analysis of clinical chemistry parameters including Alanine Aminotransferase (ALT), Alkaline phosphatase (Alk Phos), Aspartate Aminotransferase (AST), Bilirubin, Calcium, Creatinine, Direct Bilirubin, Glucose, Potassium, Protein, Sodium and Urea. Participants were counted in the worst case category that their value changes to (low, normal or high), unless there was no change in their category. Participants whose lab value category was unchanged (example given \[e.g.\], High to High), or whose value became normal, are recorded in the "To Normal or No Change" category. Participants were counted twice if the participant had values that changed 'To Low' and 'To High', so the percentages may not add to 100%. High and low indicated that the participants had values flagged as high and low respectively for the particular parameter any time on-treatment.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Urea; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
ALT; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
ALT;To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
ALT; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Alk Phos; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Alk Phos; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Alk Phos; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
AST; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
AST;To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
AST; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Bilirubin; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Bilirubin; To Normal or No Change
5 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Bilirubin; To High
1 Participants
1 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Calcium; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Calcium; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Calcium; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Creatinine; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Creatinine; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Creatinine; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Direct Bilirubin; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Direct Bilirubin; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Direct Bilirubin; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Glucose; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Glucose; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Glucose; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Potassium; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Potassium; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Potassium; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Protein; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Protein; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Protein; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Sodium; To Low
1 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Sodium; To Normal or No Change
5 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Sodium; To High
0 Participants
0 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Urea;To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Clinical Chemistry Results Relative to Normal Range
Urea; To High
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Up to 43 days

Population: Safety Population

Blood samples were collected from participants at indicated time points for the analysis of hematology parameters including Basophils, Eosinophils, Erythrocyte Mean Corpuscular Hemoglobin (MCH), Erythrocyte Mean Corpuscular Volume (MCV), Erythrocytes, Hematocrit, Hemoglobin, Leukocytes, Lymphocytes, Monocytes, Neutrophils, Platelets, Reticulocytes, and Reticulocytes/Erythrocytes. Participants were counted in the worst case category that their value changes to (low, normal or high), unless there was no change in their category. Participants whose lab value category was unchanged (e.g., High to High), or whose value became normal, are recorded in the "To Normal or No Change" category. Participants were counted twice if the participant had values that changed 'To Low' and 'To High', so the percentages may not add to 100%. High and low indicated that the participants had values flagged as high and low respectively for the particular parameter any time on-treatment.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Leukocytes; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Leukocytes; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Leukocytes; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Lymphocytes; To Low
1 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Lymphocytes; To Normal or No Change
5 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Lymphocytes; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Monocytes; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Monocytes; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Monocytes; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Neutrophils; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Neutrophils; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Neutrophils; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Platelets; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Platelets; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Platelets; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes; To High
0 Participants
1 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes/Erythrocytes; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes/Erythrocytes; To Normal or No Change
6 Participants
5 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Reticulocytes/Erythrocytes; To High
0 Participants
2 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Basophils; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Basophils; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Basophils; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Eosinophils; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Eosinophils; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Eosinophils; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCH; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCH; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCH; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCV; To Low
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCV; To Normal or No Change
6 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
MCV; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Erythrocytes; To Low
1 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Erythrocytes; To Normal or No Change
5 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Erythrocytes; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hematocrit; To Low
1 Participants
1 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hematocrit; To Normal or No Change
5 Participants
5 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hematocrit; To High
0 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hemoglobin; To Low
1 Participants
0 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hemoglobin; To Normal or No Change
5 Participants
6 Participants
Number of Participants With Worst Case Hematology Results Relative to Normal Range
Hemoglobin; To High
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Up to 43 days

Population: Safety Population

Urine samples were collected at indicated time points for the analysis of urinalysis parameters including specific gravity and PH of urine, presence of glucose, protein, blood, ketones, bilirubin, urobilinogen, nitrite, leukocyte esterase in urine.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With Abnormal Urinalysis Findings
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Pre-dose (on Day 1) and Day 8 in treatment period 2; 144 hours (Day 7) in treatment period 1

Population: Safety Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Full 12-lead ECGs were recorded with the participant in a supine position. The number of participants with abnormal ECG findings at indicated time points were presented. Data has been presented for participants with respect to the actual treatment received in respective treatment periods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Number of Participants With Abnormal Electrocardiogram (ECG) Findings
Pre-dose (Day 1); n=0,6
0 Participants
Number of Participants With Abnormal Electrocardiogram (ECG) Findings
144 hours (Day 7); n= 6, 0
0 Participants
Number of Participants With Abnormal Electrocardiogram (ECG) Findings
Day 8; n=0, 6
0 Participants

SECONDARY outcome

Timeframe: Baseline (average of Pre-dose on Day 1 in treatment period 1 and 2); 3 hours, 144 hours (Day 7) in treatment period 1; 3 hours, 144 hours (Day 7) and Day 8 in treatment period 2

Population: Safety Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Vital sign including systolic and diastolic blood pressure were measured in a semi-supine position after 5 minutes rest. Baseline is defined as the mean of the 3 pre-dose measurements (Average \[Avg\] Pre-dose) taken on Day 1 in each treatment period. Change from Baseline was defined as any visit value minus the Baseline value. Data has been presented for participants with respect to the actual treatment received in respective treatment periods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Change From Baseline in Blood Pressure
SBP; Avg 3 hours; n=6, 6
-0.17 Millimeters of mercury
Standard Deviation 5.722
-1.22 Millimeters of mercury
Standard Deviation 3.468
Change From Baseline in Blood Pressure
SBP; Avg 144 hours (Day 7); n=6, 6
5.06 Millimeters of mercury
Standard Deviation 10.521
-2.00 Millimeters of mercury
Standard Deviation 9.541
Change From Baseline in Blood Pressure
SBP; Avg Day 8; n=0,6
4.22 Millimeters of mercury
Standard Deviation 4.075
Change From Baseline in Blood Pressure
DBP; Avg 3 hours; n=6, 6
1.22 Millimeters of mercury
Standard Deviation 5.484
0.89 Millimeters of mercury
Standard Deviation 4.834
Change From Baseline in Blood Pressure
DBP; Avg 144 hours (Day 7); n=6, 6
3.61 Millimeters of mercury
Standard Deviation 9.365
-1.00 Millimeters of mercury
Standard Deviation 9.814
Change From Baseline in Blood Pressure
DBP; Avg Day 8; n=0,6
3.39 Millimeters of mercury
Standard Deviation 5.170

SECONDARY outcome

Timeframe: Baseline (average of Pre-dose on Day 1 in treatment period 1 and 2); 3 hours, 144 hours (Day 7) in treatment period 1; 3 hours, 144 hours (Day 7) and Day 8 in treatment period 2

Population: Safety Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Heart rate was measured in a semi-supine position after 5 minutes rest. Baseline is defined as the mean of the 3 pre-dose measurements (Avg Pre-dose) taken on Day 1 in each treatment period. Change from Baseline was defined as any visit value minus the Baseline value. Data has been presented for participants with respect to the actual treatment received in respective treatment periods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Change From Baseline in Heart Rate
Avg 3 hours; n=6, 6
0.22 Beats per minute
Standard Deviation 3.526
1.67 Beats per minute
Standard Deviation 3.425
Change From Baseline in Heart Rate
Avg 144 hours (Day 7); n=6, 6
1.28 Beats per minute
Standard Deviation 6.571
3.78 Beats per minute
Standard Deviation 5.500
Change From Baseline in Heart Rate
Avg Day 8; n=0,6
4.72 Beats per minute
Standard Deviation 5.535

SECONDARY outcome

Timeframe: Baseline (Pre-dose on Day 1 in treatment period 1 and 2); 3 hours, 144 hours (Day 7) in treatment period 1; 3 hours, 144 hours (Day 7) and Day 8 in treatment period 2

Population: Safety Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Respiratory rate was measured in a semi-supine position after 5 minutes rest. Baseline is defined as the pre-dose on Day 1 in each treatment period. Change from Baseline was defined as any visit value minus the Baseline value. Data has been presented for participants with respect to the actual treatment received in respective treatment periods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Change From Baseline in Respiratory Rate
3 hours; n=6, 6
-0.67 Breaths per minute
Standard Deviation 2.422
-0.33 Breaths per minute
Standard Deviation 4.082
Change From Baseline in Respiratory Rate
144 hours (Day 7); n=6, 6
-1.33 Breaths per minute
Standard Deviation 2.066
-0.33 Breaths per minute
Standard Deviation 1.506
Change From Baseline in Respiratory Rate
Day 8; n=0,6
0.00 Breaths per minute
Standard Deviation 2.530

SECONDARY outcome

Timeframe: Baseline (Pre-dose on Day 1 in treatment period 1 and 2); 3 hours, 144 hours (Day 7) in treatment period 1; 3 hours, 144 hours (Day 7) and Day 8 in treatment period 2

Population: Safety Population. Only those participants with data available at the specified data points were analyzed (represented by n= X in the category titles).

Body temperature measurement was performed in participants at indicated time points. Baseline is defined as the pre-dose on Day 1 in each treatment period. Change from Baseline was defined as any visit value minus the Baseline value. Data has been presented for participants with respect to the actual treatment received in respective treatment periods.

Outcome measures

Outcome measures
Measure
[14C]-GSK1278863 50 µg IV Infusion
n=6 Participants
Participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour in treatment period 1, after approximately 1 hour of receiving GSK1278863 6 mg Oral tablet.
[14C]-GSK1278863 25 mg Oral Solution
n=6 Participants
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
[14C]-GSK1278863 25 mg Oral Solution
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Change From Baseline in Body Temperature
3 hours; n=6, 6
0.08 Celsius
Standard Deviation 0.183
0.25 Celsius
Standard Deviation 0.455
Change From Baseline in Body Temperature
144 hours (Day 7); n=6, 6
0.07 Celsius
Standard Deviation 0.446
0.12 Celsius
Standard Deviation 0.588
Change From Baseline in Body Temperature
Day 8; n=0,6
0.30 Celsius
Standard Deviation 0.693

Adverse Events

GSK1278863 6 mg Oral Tablet+[14C]-GSK1278863 50 µg IV Infusion

Serious events: 0 serious events
Other events: 2 other events
Deaths: 0 deaths

[14C]-GSK1278863 25 mg Oral Solution

Serious events: 0 serious events
Other events: 2 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
GSK1278863 6 mg Oral Tablet+[14C]-GSK1278863 50 µg IV Infusion
n=6 participants at risk
Participants received a single 6 mg oral dose of GSK1278863 on Day 1 of treatment period 1, after an overnight fast of at least 8 hours. After approximately 1 hour, participants received 50 µg \[14C\]-GSK1278863 by IV infusion over 1 hour.
[14C]-GSK1278863 25 mg Oral Solution
n=6 participants at risk
Participants received 25 mg \[14C\]-GSK1278863 as an oral solution, after an overnight fast of at least 8 hours in treatment period 2.
Nervous system disorders
Headache
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
16.7%
1/6 • Number of events 2 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
Musculoskeletal and connective tissue disorders
Back pain
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
0.00%
0/6 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
Metabolism and nutrition disorders
Decreased appetite
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
0.00%
0/6 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
Musculoskeletal and connective tissue disorders
Musculoskeletal chest pain
0.00%
0/6 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
Musculoskeletal and connective tissue disorders
Myalgia
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
0.00%
0/6 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
0.00%
0/6 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment
16.7%
1/6 • Number of events 1 • Serious adverse events (SAEs) and non-serious adverse events (Non-SAEs) were collected from the start of the study treatment up to 43 days.
SAEs and Non-SAEs were reported by treatment for the Safety Population which comprised of all participants who had taken at least 1 dose of study treatment

Additional Information

GSK Response Center

GlaxoSmithKline

Phone: 866-435-7343

Results disclosure agreements

  • Principal investigator is a sponsor employee GSK agreements may vary with individual investigators, but will not prohibit any investigator from publishing. GSK supports the publication of results from all centers of a multi-center trial but requests that reports based on single-site data not precede the primary publication of the entire clinical trial.
  • Publication restrictions are in place

Restriction type: OTHER