Measurements of Water in Breath and Saliva

NCT ID: NCT03233256

Last Updated: 2025-12-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ENROLLING_BY_INVITATION

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-02-27

Study Completion Date

2026-12-31

Brief Summary

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This study proposes to demonstrate the feasibility of measuring 2H:1H and 18O:16O in saliva and breath by comparing the natural abundances of 2H:1H and 18O:16O in saliva and urine of un-dosed humans.

Detailed Description

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The scientific premises for this line of research are 1) challenges related to costs, the complexity of sample collection and preparation, and the level of technical skills required for Isotope-ratio mass spectrometry (IRMS) analysis have limited the adoption of the doubly labeled water (DLW) method; and 2) the investigators preliminary data demonstrates that Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS) provides a valid, more rapid, and less technically challenging approach to isotopic analysis of human samples.

Conditions

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Human Energy Expenditure

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Healthy adults (18-45 yrs.) will be recruited from the local Denver area. The investigators have elected to study a relatively homogenous sample to limit the impact of age, weight, and chronic disease on isotope fractionation in breath.
Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Healthy adults (18-45 yrs.)

Healthy adults (18-45 yrs.) will be recruited from the local Denver area. The investigators have elected to study a relatively homogenous sample to limit the impact of age, weight, and chronic disease on isotope fractionation in breath.

Group Type EXPERIMENTAL

Feasibility of measuring 2H:1H and 18O:16O in saliva and breath.

Intervention Type PROCEDURE

The investigators propose to demonstrate the feasibility of measuring 2H:1H and 18O:16O in saliva and breath. The natural abundances of 2H:1H and 18O:16O of urine, saliva, and breath samples will be determined from samples obtained simultaneously from 50 relatively healthy human subjects. We will also measure 2H:1H and 18O:16O enrichments in urine, saliva, and breath of 10 relatively healthy human subjects obtained after consuming a standard DLW dose and compare the estimates of TDEE using these different matrices.

Interventions

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Feasibility of measuring 2H:1H and 18O:16O in saliva and breath.

The investigators propose to demonstrate the feasibility of measuring 2H:1H and 18O:16O in saliva and breath. The natural abundances of 2H:1H and 18O:16O of urine, saliva, and breath samples will be determined from samples obtained simultaneously from 50 relatively healthy human subjects. We will also measure 2H:1H and 18O:16O enrichments in urine, saliva, and breath of 10 relatively healthy human subjects obtained after consuming a standard DLW dose and compare the estimates of TDEE using these different matrices.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Healthy adult men and women, aged 18-45 yrs

Exclusion Criteria

* Obesity (body mass index (BMI) \> 30 kg/m2)
* Pregnant or lactating women
* Self-reported acute or chronic disease (diabetes, heart diseases, thyroid diseases)
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

NIH

Sponsor Role collaborator

University of Colorado, Denver

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Edward L Melanson, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Colorado, Denver

Locations

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Edward Melanson

Aurora, Colorado, United States

Site Status

Countries

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United States

Other Identifiers

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R21DK113401

Identifier Type: NIH

Identifier Source: secondary_id

View Link

16-2203

Identifier Type: -

Identifier Source: org_study_id