Ethanol Induces Skeletal Muscle Autophagy

NCT ID: NCT03209791

Last Updated: 2026-01-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-05-18

Study Completion Date

2027-12-31

Brief Summary

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In this study we plan to demonstrate that ethanol induces skeletal muscle autophagy to degrade MAA adducts.

Detailed Description

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Hypothesis: Ethanol mediated modification of skeletal muscle proteins to form MAA adducts that in turn induce skeletal muscle autophagy.

Patients with alcoholic steatosis, hepatitis and cirrhosis (n=10 each) will be recruited from the liver transplant nutrition clinic or the hepatology inpatient service and their body composition quantified using anthropometry, bioelectrical impedance analysis, CT image analysis and DEXA if available.

Control Subjects. Controls will be recruited by advertisement. All control subjects will have a normal clinical history, physical examination, and screening chemistries. They will not have any significant medical conditions requiring the use of medications. Their nutritional status within 20% of normal as defined by ideal body weight.

Conditions

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Alcoholic Liver Disease

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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Alcoholic Steatosis

This group will have 10 patients with alcoholic steatosis which is milder disease and muscle biopsies will be performed

Biopsies

Intervention Type OTHER

Biopsy will be done on the Vastus Lateralis muscle in all groups

Cirrhosis

This group will consist of 10 patients with cirrhosis. We anticipate a 50% difference in these patients and the controls in the autophagy readouts and muscle biopsies will be performed

Biopsies

Intervention Type OTHER

Biopsy will be done on the Vastus Lateralis muscle in all groups

Steatohepatitis

This group will have 10 patients with alcoholic steatohepatitis that have more severe necroinflammation in the liver but for a shorter duration of illness and muscle biopsies will be performed

Biopsies

Intervention Type OTHER

Biopsy will be done on the Vastus Lateralis muscle in all groups

controls

This group will consist of 10 patients who are healthy and have no liver disease diagnosis and muscle biopsies will be performed

Biopsies

Intervention Type OTHER

Biopsy will be done on the Vastus Lateralis muscle in all groups

Interventions

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Biopsies

Biopsy will be done on the Vastus Lateralis muscle in all groups

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

Alcoholic liver disease:

* clinical, biochemical, imaging criteria and liver biopsy where available
* Age 18 - 65 years old

Controls:

* Serum liver transaminases (i.e. ALT and AST) 40 IU/L
* Normal liver ultrasound
* Age 18 - 65 years old

Exclusion Criteria

For both groups - alcoholic liver disease and controls:

* Poorly controlled diabetes mellitus (HbA1C\>9.5 g/dl)
* Untreated Hyper- / hypo- thyroidism
* Patients on dialysis, renal disease with serum creatinine 1.5 mg/dL
* Active intravenous drug use
* History of bowel surgery or gastric bypass surgery
* Medications known to alter muscle protein metabolism (i.e. corticosteroids, tamoxifen, high-dose estrogen, testosterone or anabolic steroids)
* Metastatic disease, Advanced cardiac or pulmonary disease
* Pregnancy
* Coagulopathy- INR \>1.4 and platelet count \<80,000/ml.
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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The Cleveland Clinic

OTHER

Sponsor Role lead

Responsible Party

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Srinivasan Dasarathy

Staff Physician

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Srinivasan Dasarathy, MD

Role: PRINCIPAL_INVESTIGATOR

The Cleveland Clinic

Locations

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Cleveland Clinic

Cleveland, Ohio, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Revathi Penumatsa, MPH

Role: CONTACT

2164450688

Annette Bellar, MSLA

Role: CONTACT

2166365247

Facility Contacts

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Revathi Penumatsa

Role: primary

216-445-0688

Annette Bellar

Role: backup

2166365247

Other Identifiers

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14-1287

Identifier Type: -

Identifier Source: org_study_id

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