Adoptive Transfer of Specific HCC Antigens CD8+ T Cells for Treating Patients With Relapsed/Advanced HCC

NCT ID: NCT03175705

Last Updated: 2018-01-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE1

Total Enrollment

18 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-05-01

Study Completion Date

2019-03-30

Brief Summary

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This study enrolls patients who have relapsed/advanced hepatocellular carcinoma (HCC, BCLC stage C). The HCC tumor relapsed or metastasized through the body after standard treatment or the patients cannot receive standard treatment under current conditions. This research study uses specific HCC antigens CD8+ T cells, a new experimental treatment.

The purpose of this study is to evaluate the safety and tolerance as well as the potential clinical efficacy of an adoptive transfer of CD8+ T cells, sorted with human leukocyte antigen (HLA)-peptide multimers and specific for Glypican (GPC)-3 /New York Esophageal Squamous-1 (NY-ESO-1) /alpha-fetoprotein (AFP) antigens and cultured in vitro, to patients suffering from relapsed/advanced hepatocellular carcinoma (HCC).

Detailed Description

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Conditions

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Hepatocellular Carcinoma

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Autologous T cell therapy+Tegafur+Interleukin-2 (IL-2)

Autologous in vitro expanded HCC antigens-specific CD8+ T lymphocytes in conjunction with IL-2 and along with lymphodepleting chemotherapy (Tegafur) will be administered to patients with relapsed/advanced HCC.

Group Type EXPERIMENTAL

HCC antigens-specific CD8+ T lymphocytes

Intervention Type BIOLOGICAL

Three different dosing schedules will be evaluated. Three patients will be evaluated on each dosing schedule.

The following dose levels will be evaluated:

Loading Dose 1: 3x10\^7/m2 Loading Dose 2: 6x10\^7/m2 Loading Dose 3: 9x10\^7/m2 The doses are calculated according to the actual number of GPC3/NY-ESO-1/AFP specific cytotoxic lymphocytes (CTLs)

IL-2

Intervention Type DRUG

IL-2 will be given at a dose of 25000 IU/kg/day for 5-14 days.

Tegafur

Intervention Type DRUG

Tegafur will be given at a dose of 40\~60 mg bis in die (BID) 2 weeks.

Interventions

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HCC antigens-specific CD8+ T lymphocytes

Three different dosing schedules will be evaluated. Three patients will be evaluated on each dosing schedule.

The following dose levels will be evaluated:

Loading Dose 1: 3x10\^7/m2 Loading Dose 2: 6x10\^7/m2 Loading Dose 3: 9x10\^7/m2 The doses are calculated according to the actual number of GPC3/NY-ESO-1/AFP specific cytotoxic lymphocytes (CTLs)

Intervention Type BIOLOGICAL

IL-2

IL-2 will be given at a dose of 25000 IU/kg/day for 5-14 days.

Intervention Type DRUG

Tegafur

Tegafur will be given at a dose of 40\~60 mg bis in die (BID) 2 weeks.

Intervention Type DRUG

Other Intervention Names

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Human recombinated Interleukin-2 Gimeracil and Oteracil potassium Capsules

Eligibility Criteria

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Inclusion Criteria

* Age 18-80 years
* Patients with relapsed/advanced HCC (BCLC, stage C) proved by histopathology or proved by CT or MRI imaging system, proven GPC3/NY-ESO-1/AFP(+), relapsed after previous therapy and no effective therapies known at this time.
* Life expectancy of ≥ 12 weeks.
* WBC\>3.5×10\^9/L, LYMPH\> 0.8×10\^9/L, Hb\>85g/L, PLT\>50×10\^9/L, Cre\<1.5×the upper limit of normal value.
* Able to understand and sign the informed consent.

Exclusion Criteria

* Any uncontrolled systematic disease: hypertension, heart disease, and et al.;
* Portal vein tumor thrombus, central nervous system tumor metastasis, or combined with other tumors;
* Receiving radiochemotherapy, local therapy, or targeting drugs within 4 weeks prior to this treatment;
* Unstable immune systematic diseases or Infectious diseases;
* Combined with AIDS or syphilis;
* Patients with history of stem cell or organ transplantation;
* Patients with allergic history to related drugs and immunotherapy;
* Patients with complications associated with liver diseases: moderate or severe pleural effusion, pericardial effusion, ascites, or gastrointestinal hemorrhage;
* Pregnant or lactating subjects;
* Unsuitable subjects considered by clinicians.
Minimum Eligible Age

18 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Beijing YouAn Hospital

OTHER

Sponsor Role lead

Responsible Party

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LU JUN

Director of Hepatology and Cancer Biotherapy Ward

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Beijing Youan Hospital,Capital Medical University

Beijing, Beijing Municipality, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Jun Lu, Director

Role: CONTACT

86-13661381489

Xuli Bao, Clinician

Role: CONTACT

86-13161935299

Facility Contacts

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Xuli Bao, Clinician

Role: primary

86-13161935299

Other Identifiers

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Beijing Youan Ethics[2017]06

Identifier Type: -

Identifier Source: org_study_id

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