Atopic Dermatitis (AD) and Food Allergy

NCT ID: NCT03168113

Last Updated: 2018-02-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

62 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-06-12

Study Completion Date

2018-01-31

Brief Summary

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This is a prospective, single center, clinical mechanistic pilot clinical research study. Participants will not receive any investigational agent. The investigators will examine whether children with atopic dermatitis (AD) and food allergy have a different skin barrier, microbiome, epidermal transcriptome, and epidermal lipid composition than children with AD and no food allergy and non-atopic (NA) children. Participation involves a single study visit.

Detailed Description

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Atopic Dermatitis (AD) is a chronic inflammatory skin disorder in which the skin becomes extremely itchy and is susceptible to recurrent skin infections. AD is thought to occur from a combination of immunological, genetic, and environmental factors.

Those with atopic dermatitis (AD) often have food allergy and Staphylococcus aureus (S. aureus) colonization of the skin. There is evidence suggesting that skin barrier dysfunction, measurable as increased transepidermal water loss (TEWL), is a predisposing factor to food sensitization and food allergy from epicutaneous penetration of environmental food allergens. Furthermore, the investigators for this study have identified that AD children with food allergy, especially peanut allergy, are colonized with Staphylococcus aureus. However, only half (50%) of children with AD have food allergy or S. aureus colonization, suggesting there are other factors accounting for food allergy. There have been no previous studies of TEWL or, microbial or molecular profiling of the skin in those with AD prone to food allergy versus AD without food allergy.

Conditions

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Atopic Dermatitis (AD) Non-atopic Healthy Controls

Keywords

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skin barrier function microbiome transepidermal water loss (TEWL) epidermal transcriptome epidermal lipid composition

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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AD and Peanut Food Allergy

Participants with active Atopic Dermatitis (AD) and food allergy to peanut.\* Twenty participants ages 4 to 17 years of age will be enrolled in this group.

\*Peanut skin prick test wheal ≥ 8 mm.

No interventions assigned to this group

AD and No Food Allergy

Participants with active Atopic Dermatitis (AD) and no food allergy.\* Twenty participants ages 4 to 17 years of age will be enrolled in this group.

\*Negative skin prick test (wheal \< 3 mm) to peanut, milk, egg, wheat, soy, shellfish mix, tree nuts, and sesame seed.

No interventions assigned to this group

Non-Atopic Health Control

Participants that are non-atopic, healthy controls\*. Twenty participants ages 4 to 17 years of age will be enrolled in this group.

\*Negative for Atopic Dermatitis (AD), asthma, or allergic rhinitis; negative for food allergy; and negative by skin prick test to peanut, milk, egg, wheat, soy, shellfish mix, tree nuts, and sesame seed, and negative to environmental allergens - cat, dog, dust mite, cockroach, and local trees/grasses/weeds/molds.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

Participants fulfilling all of the following criteria are eligible for enrollment-

-Parent/guardian must be able to understand and provide informed consent and participant provide assent as applicable per Institutional Review Board (IRB) guidelines and regulations;

For Eligibility to One of the Two Active Atopic Dermatitis (AD) Groups:

-Active Atopic Dermatitis (AD) without a history or current manifestations of eczema herpeticum (EH), as diagnosed using the Atopic Dermatitis Registry

Network (ADRN) Standard Diagnostic Criteria and food allergy to peanut. Participant must meet all of the following criteria:

* Self-report or documentation of a positive oral food challenge to peanut or self-report of an allergic reaction to peanut within 2 hours of ingestion
* Peanut skin prick test wheal ≥ 8 mm. OR -Active AD without a history or current manifestations of EH, as diagnosed using ADRN Standard Diagnostic Criteria and no food allergy.

Participant must meet all of the following criteria:

* No personal history or current manifestations of food allergy (based on no self-report of a positive oral food challenge, positive skin test, positive blood test, or allergic reactions).
* Negative skin prick test (wheal \< 3 mm) to peanut, milk, egg, wheat, soy, shellfish mix, tree nuts, and sesame seed.

For Non-atopic (NA) Group Eligibility:

Participant must meet all of the following criteria:

* No personal history or current manifestations of AD, asthma, or allergic rhinitis (based on self-report);
* No personal history or current manifestations of food allergy (based on no self- report of a positive oral food challenge, positive skin test, positive blood test, or allergic reactions);
* Negative skin prick test (wheal \< 3 mm) to peanut, milk, egg, wheat, soy, shellfish mix, tree nuts, and sesame seed; and
* Negative skin prick test (wheal \< 3 mm) to environmental allergens (cat, dog, dust mite, cockroach, and local trees/grasses/weeds/molds).

Exclusion Criteria

* Inability or unwillingness of a parent/guardian to give written informed consent, or participant to give assent, if applicable, or to comply with study protocol;
* Subjects with skin disease other than AD that might compromise the stratum corneum barrier (e.g., bullous diseases, psoriasis, cutaneous T cell lymphoma \[also called Mycosis Fungoides or Sezary syndrome\], dermatitis herpetiformis, Hailey- Hailey, or Darier's disease);
* Pregnant or lactating females;
* Known or suspected immunosuppression;
* Severe concomitant illness(es);
* History of serious life-threatening reaction to latex, tape, or adhesives;
* Past or current medical problems or findings from physical examination or laboratory testing that are not listed above, which, in the opinion of the investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or that may impact the quality or interpretation of the data obtained from the study;
* Use of biologics within 5 half-lives (if known) or 16 weeks of the Screening Visit;
* Use of an investigational drug within 5 half-lives (if known) or 8 weeks of the Screening Visit; or
* Has received immunotherapy within 12 months of the Screening Visit.
Minimum Eligible Age

4 Years

Maximum Eligible Age

17 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Atopic Dermatitis Research Network

OTHER

Sponsor Role collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Donald Leung, M.D., Ph.D.

Role: PRINCIPAL_INVESTIGATOR

National Jewish Health: Division of Pediatric Allergy and Clinical Immunology

Locations

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National Jewish Health: Division of Pediatric Allergy and Clinical Immunology

Denver, Colorado, United States

Site Status

Countries

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United States

References

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Lyubchenko T, Collins HK, Goleva E, Leung DYM. Skin tape sampling technique identifies proinflammatory cytokines in atopic dermatitis skin. Ann Allergy Asthma Immunol. 2021 Jan;126(1):46-53.e2. doi: 10.1016/j.anai.2020.08.397. Epub 2020 Sep 5.

Reference Type DERIVED
PMID: 32896640 (View on PubMed)

Related Links

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http://www.niaid.nih.gov/

National Institute of Allergy and Infectious Diseases (NIAID) Website

Other Identifiers

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DAIT ADRN-10

Identifier Type: -

Identifier Source: org_study_id