Trial Outcomes & Findings for Propranolol Hydrochloride in Treating Patients With Prostate Cancer Undergoing Surgery (NCT NCT03152786)

NCT ID: NCT03152786

Last Updated: 2025-02-17

Results Overview

Determined using western blot in prostate tissue from men. CREB is a type of protein that binds to DNA in very specific places. CREB is related to long-term memory formation in the brain and is important in the formation of spatial memory. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or otherwise modifying its function. The level of CREB phosphorylation is an indicator of BAD activity.

Recruitment status

TERMINATED

Study phase

PHASE2

Target enrollment

46 participants

Primary outcome timeframe

2 hours after taking or not taking propranolol prior to prostatectomy

Results posted on

2025-02-17

Participant Flow

January 15, 2019 to June 8, 2021

Participant milestones

Participant milestones
Measure
Group I (Propranolol Hydrochloride)
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Overall Study
STARTED
23
23
Overall Study
COMPLETED
21
22
Overall Study
NOT COMPLETED
2
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Group I (Propranolol Hydrochloride)
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Overall Study
Withdrawal by Subject
2
1

Baseline Characteristics

Propranolol Hydrochloride in Treating Patients With Prostate Cancer Undergoing Surgery

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Group I (Propranolol Hydrochloride)
n=23 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=23 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Total
n=46 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
8 Participants
n=5 Participants
9 Participants
n=7 Participants
17 Participants
n=5 Participants
Age, Categorical
>=65 years
15 Participants
n=5 Participants
14 Participants
n=7 Participants
29 Participants
n=5 Participants
Age, Continuous
68.35 years
STANDARD_DEVIATION 6.21 • n=5 Participants
64.61 years
STANDARD_DEVIATION 64.61 • n=7 Participants
66.48 years
STANDARD_DEVIATION 6.71 • n=5 Participants
Sex: Female, Male
Female
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Sex: Female, Male
Male
23 Participants
n=5 Participants
23 Participants
n=7 Participants
46 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
6 Participants
n=5 Participants
5 Participants
n=7 Participants
11 Participants
n=5 Participants
Race (NIH/OMB)
White
15 Participants
n=5 Participants
18 Participants
n=7 Participants
33 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
2 Participants
n=5 Participants
0 Participants
n=7 Participants
2 Participants
n=5 Participants
Region of Enrollment
United States
23 participants
n=5 Participants
23 participants
n=7 Participants
46 participants
n=5 Participants

PRIMARY outcome

Timeframe: 2 hours after taking or not taking propranolol prior to prostatectomy

Population: levels of total CREB signal were below detection limit in missing participants

Determined using western blot in prostate tissue from men. CREB is a type of protein that binds to DNA in very specific places. CREB is related to long-term memory formation in the brain and is important in the formation of spatial memory. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or otherwise modifying its function. The level of CREB phosphorylation is an indicator of BAD activity.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=6 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=5 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Mean Level of CREB Phosphorylation
1.34 West blot band intensity,arbitrary units
Standard Deviation .33
1.43 West blot band intensity,arbitrary units
Standard Deviation .46

SECONDARY outcome

Timeframe: 2 hours after taking or not taking propranolol prior to prostatectomy

Population: BAD phosphorylation not detected

Will be assessed by western blot in prostate tissue from men. BAD is a protein that plays a critical role in cell survival. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or otherwise modifying its function. This analysis shows how much phosphorylation of BAD protein is happening in cells.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=21 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=22 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
BAD Phosphorylation
0 kDa
Standard Deviation 0
0 kDa
Standard Deviation 0

SECONDARY outcome

Timeframe: On the day of surgery before taking propranolol, prior to prostatectomy

Will be measured by The Distress Thermometer from men. The NCCN Distress Thermometer (DT) is a one-item, 11-point Likert scale represented on a visual graphic of a thermometer that ranges from 0 (no distress) to 10 (extreme distress), with which patients indicate their level of distress over the course of the week prior to assessment. Scoring scale ranges from 0 (no distress) to 10 (extreme distress) with higher scores indicating more distress.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=20 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=23 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Distress Score - National Comprehensive Cancer Network Distress Thermometer Questionnaire
3.6 score on a scale
Standard Deviation 2.3
2.7 score on a scale
Standard Deviation 2.6

SECONDARY outcome

Timeframe: 2 hours after taking or not taking propranolol prior to prostatectomy

Population: insufficient material for RNA extraction

Difference in levels of transcripts that reflect ADRB2/PKA activation will be measured by real-time polymerase chain reaction in prostate tissue from men.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=21 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=22 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Levels of Transcripts That Reflect ADRB2/PKA Activation
NA relative gene expression
Standard Deviation NA
insufficient material for RNA extraction
NA relative gene expression
Standard Deviation NA
insufficient material for RNA extraction

SECONDARY outcome

Timeframe: 2 hours after taking or not taking propranolol prior to prostatectomy

Population: one blood sample was missing during Epi measurement

Will be measured by enzyme-linked immunosorbent assay from men. 2-sample t-tests will be calculated to compare the groups who were randomized to receive propranolol (yes/no). Descriptive statistics will be calculated for each measure within each group. These statistics include n, mean, standard deviations and 95% confidence intervals.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=7 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=8 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Plasma Catecholamine Levels (Including Epinephrine)
0.36 nano mole (nm) per liter (L)
Standard Deviation 0.22
0.34 nano mole (nm) per liter (L)
Standard Deviation 0.22

SECONDARY outcome

Timeframe: 2 hours after taking or not taking propranolol prior to prostatectomy

Population: Number of samples collected for analysis.

Will be measured by fluorometric detection from men. 2-sample t-tests will be calculated to compare the groups who were randomized to receive propranolol (yes/no). Descriptive statistics will be calculated for each measure within each group. These statistics include n, mean, standard deviations and 95% confidence intervals.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=8 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=8 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Plasma Propranolol Levels
10.89 nanograms per milliliter (ng/ml)
Standard Deviation 4.58
0.00 nanograms per milliliter (ng/ml)
Standard Deviation 0.00

SECONDARY outcome

Timeframe: On the day of surgery before taking propranolol, prior to prostatectomy

Will be measured by The Perceived Stress Questionnaire from men. The perceived stress questionnaire is a set of 4 questions asking participants about their feelings/problems with stress over the last two weeks. Scoring scale ranges from 0 to 16 with higher scores indicating a higher level of stress.

Outcome measures

Outcome measures
Measure
Group I (Propranolol Hydrochloride)
n=20 Participants
Patients receive propranolol hydrochloride PO 2 hours prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Propranolol Hydrochloride: Given PO Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Group II (no Treatment)
n=23 Participants
Patients receive no treatment prior to standard of care prostatectomy. Laboratory Biomarker Analysis: Correlative studies Questionnaire Administration: Ancillary studies Survey Administration: Ancillary studies
Self-perceived Stress
7.4 score on a scale
Standard Deviation 2.3
8.2 score on a scale
Standard Deviation 1.4

Adverse Events

Group I (Propranolol Hydrochloride)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Group II (no Treatment)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Ashok Hemal, MD

Wake Forest School of Medicine

Phone: 336-716-5694

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place