Reconstitution of a Human Immune System in a Patient Derived Xenograft (PDX) Model of Genitourinary (GU) Cancers

NCT ID: NCT03134027

Last Updated: 2024-07-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Total Enrollment

3 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-10-19

Study Completion Date

2018-05-09

Brief Summary

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The objective of this study is to obtain human blood CD34+ hematopoietic stem/progenitor cells (HSPCs) to reconstitute a match human immune system in our PDX model. The hypothesis is that by using matched leukocytes and PDX from the same patient, rejection of the PDX by the host immune system will not be observed and therefore a preclinical model to study immunotherapy can be developed to study, understand and improve upon our current therapies. HSPCs will be collected from bone marrow aspirate obtained from a bone marrow biopsy.

The secondary objective is to use patient tumor biopsy samples or circulating tumor cell samples to develop additional preclinical models of GU cancers, particularly prostate cancer, that are clinically relevant by generating additional PDXs.

Detailed Description

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Conditions

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Genito Urinary Cancer Bladder Cancer Kidney Cancer Prostate Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Subjects from which PDXs have been generated.

Subjects will be identified from which PDXs have been generated from an already approved IRB protocol.

Bone marrow biopsy

Intervention Type PROCEDURE

Subjects will undergo a bone marrow biopsy to obtain aspirate for stem cell collection.

Subjects without an existing PDX

Subjects with prostate cancer amenable to a tumor biopsy.

Tumor biopsy

Intervention Type PROCEDURE

Subjects will undergo a tumor biopsy to obtain tissue for generation of a PDX.

Interventions

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Bone marrow biopsy

Subjects will undergo a bone marrow biopsy to obtain aspirate for stem cell collection.

Intervention Type PROCEDURE

Tumor biopsy

Subjects will undergo a tumor biopsy to obtain tissue for generation of a PDX.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

1. Patients with Genitourinary (GU) Cancers and presence of existing patient derived xenograft or planned collection of tumor for PDX generation
2. Age ≥ 18 years.
3. Ability to understand and provide signed informed consent that fulfills Institutional Review Board guidelines.
4. Ability to undergo bone marrow biopsy
5. Laboratory requirements (Hematocrit 30%, Platelets 75,000 + 10\^6/l and WBC 4000 X 10\^6/l)
6. Karnofsky performance status \>70%


1. Patients with prostate cancer

a. Biopsies in this study for diseases other than prostate cancer are not permitted.
2. Age ≥ 18 years.
3. Ability to understand and provide signed informed consent that fulfills Institutional Review Board guidelines.
4. Planned or willing to undergo a tumor biopsy. Biopsies may be for research or clinical purposes depending on the treating provider for men with prostate cancer. Biopsies in this study for diseases other than prostate cancer are not permitted

1. For research only biopsies: At least one lymph node or bone metastatic amenable to a minimum risk biopsy in the opinion of the treating physician.
2. For clinical purpose biopsies: There are no location or risk requirements.
5. Laboratory requirements (Hematocrit 30%, Platelets 75,000 X 10\^6/l and WBC 4000 X 10\^6/l)
6. Karnofsky performance status \>70%

Exclusion Criteria

1\. Participants with serious concurrent chronic or acute illness that would affect the safety of a bone marrow biopsy.


1\. Participants with serious concurrent chronic or acute illness that would affect the safety of a tumor biopsy.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Duke University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Andrew Armstrong, MD, ScM

Role: PRINCIPAL_INVESTIGATOR

Duke Cancer Institute

Locations

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Duke University Medical Center

Durham, North Carolina, United States

Site Status

Countries

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United States

Other Identifiers

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Pro00082398

Identifier Type: -

Identifier Source: org_study_id

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