Dysregulation of Glutamine Activity in the Pathogenesis of Multiple Myeloma

NCT ID: NCT03119883

Last Updated: 2024-08-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

35 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-06-02

Study Completion Date

2024-06-30

Brief Summary

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This clinical trial studies the utilization of glutamine by the bone marrow plasma cells from patients with monoclonal gammopathy of undetermined significance (MGUS) compared to multiple myeloma (MM). Results from this study may identify metabolic differences between pre-malignant and malignant clonal plasma cells in MGUS and MM, respectively. It may also allow researchers better determine the transition from MGUS to MM for the development of potential early diagnostic purposes of preventative strategies.

Detailed Description

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PRIMARY OBJECTIVE:

I. Compare the glutamine anaplerosis activity in bone marrow clonal plasma cells (cPCs) between patients with MGUS and MM using an in vivo and an ex vivo approach.

SECONDARY OBJECTIVES:

I. Compare the tricarboxylic acid (TCA) isotopomer enrichment in the bone marrow plasma obtained from patients with MGUS and MM with the in vivo approach.

II. Determine the peripheral blood enrichment of 13C in the TCA cycle intermediates from patients with MGUS and MM with the in vivo approach.

OUTLINE: Patients are assigned to 1 of 2 groups.

GROUP I (EX-VIVO): After an overnight fast of 6 hours, patients undergo collection of blood samples.

GROUP II (IN VIVO): After an overnight fast of 6 hours, patients receive 13-carbon labeled glutamine or 13-carbon-labeled glucose intravenously (IV) over 90 minutes. Patients also undergo collection of blood and bone marrow aspirate samples.

After completion of study, patient are followed up for 4 weeks.

Conditions

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Monoclonal Gammopathy of Undetermined Significance Plasma Cell Myeloma

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Group I (biospecimen collection)

EX-VIVO: After an overnight fast of 6 hours, patients undergo collection of bone marrow aspirate samples.

Group Type EXPERIMENTAL

Biospecimen Collection

Intervention Type PROCEDURE

Undergo collection of bone marrow aspirate samples

Group II (biospecimen collection, glutamine, glucose)

IN VIVO: After an overnight fast of 6 hours, patients receive 13-carbon labeled glutamine or 13-carbon-labeled glucose IV over 90 minutes. Patients also undergo collection of blood and bone marrow aspirate samples.

Group Type EXPERIMENTAL

5-Carbon C 13-labeled Glutamine

Intervention Type OTHER

Given IV

Biospecimen Collection

Intervention Type PROCEDURE

Undergo collection of blood

Biospecimen Collection

Intervention Type PROCEDURE

Undergo collection of bone marrow aspirate samples

Uniformly-labeled [13C]glucose

Intervention Type OTHER

Given IV

Interventions

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5-Carbon C 13-labeled Glutamine

Given IV

Intervention Type OTHER

Biospecimen Collection

Undergo collection of blood

Intervention Type PROCEDURE

Biospecimen Collection

Undergo collection of bone marrow aspirate samples

Intervention Type PROCEDURE

Uniformly-labeled [13C]glucose

Given IV

Intervention Type OTHER

Other Intervention Names

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5-13C-Glutamine [5-13C]-glutamine GLUTAMINE 5-C-13 Biological Sample Collection Biospecimen Collected Specimen Collection Biological Sample Collection Biospecimen Collected Specimen Collection [U-13C] Glucose U-13C-Glucose

Eligibility Criteria

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Inclusion Criteria

* At least 18 years of age
* International Myeloma Working Group (IMWG) criteria for the diagnosis of either MGUS or MM

Exclusion Criteria

* Unable to provide consent
* Eastern Cooperative Oncology Group (ECOG) performance status (PS) \> 3
* Hemoglobin \< 8 g/dL
* Women who are pregnant
* Prior history of adverse events with conscious sedation
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Mayo Clinic

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Wilson I Gonsalves, M.D.

Role: PRINCIPAL_INVESTIGATOR

Mayo Clinic in Rochester

Locations

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Mayo Clinic in Rochester

Rochester, Minnesota, United States

Site Status

Countries

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United States

References

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Gonsalves WI, Jang JS, Jessen E, Hitosugi T, Evans LA, Jevremovic D, Pettersson XM, Bush AG, Gransee J, Anderson EI, Kumar SK, Nair KS. In vivo assessment of glutamine anaplerosis into the TCA cycle in human pre-malignant and malignant clonal plasma cells. Cancer Metab. 2020 Dec 11;8(1):29. doi: 10.1186/s40170-020-00235-4.

Reference Type DERIVED
PMID: 33308307 (View on PubMed)

Related Links

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Other Identifiers

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NCI-2021-02642

Identifier Type: REGISTRY

Identifier Source: secondary_id

K23CA218742

Identifier Type: NIH

Identifier Source: secondary_id

View Link

17-003133

Identifier Type: -

Identifier Source: org_study_id

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