Microbiome and Sarcopenia in Patients With Liver Cirrhosis

NCT ID: NCT03080129

Last Updated: 2024-10-09

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: NA
• Total Enrollment: 120 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2017-04-11
• Study Completion Date: 2026-12-31

Brief Summary

Protein-energy malnutrition (PEM) occurs in 65-90% of patients with liver cirrhosis. Severity of malnutrition correlates with progression of liver disease and leads to sarcopenia in 30-70% of cirrhotic patients. Malnutrition and sarcopenia are associated with an increased risk of complications and mortality.

In cirrhosis the gut microbiome is altered leading to increased gut permeability, bacterial translocation and inflammation. Since the microbiome is involved in nutrient uptake and metabolism, it is hypothesized that microbiome alterations contribute to sarcopenia. A prospective controlled cohort study to investigate the interrelation of microbiome changes and sarcopenia in cirrhosis will be conducted. Furthermore the effect of nutritional interventions on the microbiome in cirrhosis will be studied. From this study information on how the gut microbiome composition and sarcopenia are associated in cirrhosis and if modulation of the gut microbiome by nutritional interventions is feasible will be collected.

Detailed Description

Scientific background

Protein-energy malnutrition (PEM) occurs in 65-90% of patients with chronic liver disease. PEM is caused by various factors including poor dietary intake, loss of appetite, decreased hepatic protein synthesis, malabsorption and hypermetabolism. It is associated with an increased risk of complications including ascites, hepatic encephalopathy, variceal bleeding, hepatorenal syndrome and mortality. There is a direct relation between the progression of the liver disease and the severity of malnutrition.

Malnutrition and sarcopenia in liver cirrhosis patients

PEM leads to sarcopenia as a common, but frequently overlooked, complication. Sarcopenia is defined as a decrease in muscle mass two standard deviations below the healthy young adult mean. Sarcopenia is associated with aging, chronic diseases and malignancy. To determine the severity of muscle wasting, computed tomography scan (CT) or magnetic resonance imaging (MRI) are an objective and reproducible technique. Sarcopenia negatively impacts on survival, correlates with the risk of infections, increases surgical risk and leads to a poor quality of life. Besides PEM also inflammation is of importance in the development of sarcopenia.

Diversity in the microbiome in patients with liver cirrhosis and association with sarcopenia.

The gut microbiome of liver cirrhosis patients is altered compared to healthy individuals. Dysbiosis leads to an increased gut permeability, bacterial translocation and inflammation. This contributes to fibrogenesis and may also be related to hepatocarcinogenesis. Hence, new treatment approaches in cirrhosis focus on changing the microbial landscape.

Modulation the gut microbiome may also be a strategy to reverse sarcopenia by reducing systematic inflammation.

Hypothesis and aims

There is an association between gut microbiome composition, gut permeability and the existence of sarcopenia in cirrhotic patients.

Primary hypothesis: Diversity of the gut microbiome is reduced in liver cirrhosis patients with sarcopenia compared to those without sarcopenia or healthy controls.

Secondary hypotheses: There is an association between gut microbiome composition, biomarker of gut permeability and bacterial translocation with the presence of sarcopenia in cirrhosis. Oral nutrition supplements (ONS) can influence the composition of the gut microbiome, gut permeability, bacterial translocation and inflammation. Sarcopenia can be diagnosed from patients portraits.

Aims: to investigate:

• the composition of the gut microbiome
• biomarkers of gut permeability, bacterial translocation and inflammation
• the incidence and severity of sarcopenia
• the impact of oral nutrition supplements (ONS) on the gut microbiome
• whether artificial intelligence can be used to diagnose sarcopenia from face portraits.

Conditions

Sarcopenia; Liver Cirrhosis

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: SINGLE

Study Groups

Cirrhosis + sarcopenia

Patients with cirrhosis will receive 200ml of an oral nutritional supplement daily for 7 days.

Group Type: EXPERIMENTAL

Fresubin energy

Intervention Type: DIETARY_SUPPLEMENT

dietary protein energy supplement

Control

Patients with sarcopenia and no evidence of cirrhosis and healthy controls

Group Type: NO_INTERVENTION

No interventions assigned to this group

Interventions

Fresubin energy

dietary protein energy supplement

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• Hospitalized patients for any reason with clinical/radiological/histological diagnosis of cirrhosis
• Age >18y
• Informed consent
• CT/MRI scan within +/-14 days of the baseline study visit

Exclusion Criteria

• Hepatic encephalopathy > grade 2 and or other cognitive disorder not allowing informed consent
• advanced hepatocellular carcinoma
• Any other condition or circumstance, which, in the opinion of the investigator, would affect the patient's ability to participate in the protocol

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 100 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Medical University of Graz

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Medical University Graz

Graz, , Austria

Site Status: RECRUITING

Countries

Austria

Central Contacts

Vanessa Stadlbauer-Köllner, AssocProf Dr

Role: CONTACT

vanessa.stadlbauer@medunigraz.at

+4331638582282

Julia Haberl, BSc

Role: CONTACT

julia.haberl@klinikum-graz.at

+4331638580777

Facility Contacts

Vanessa Stadlbauer-Köllner, MD

Role: primary

vanessa.stadlbauer@medunigraz.at

0043 316 385 ext. 82282

References

Woltsche J, Pacher-Deutsch C, Furst S, Gulden L, Schwarzl J, Feldbacher N, Nepel M, Celcer L, Hasl N, Rieper V, Stadlbauer V, Horvath A. Microbial Dysbiosis in the Urinary Microbiome of Patients With Cirrhosis. Am J Gastroenterol. 2025 Jul 7. doi: 10.14309/ajg.0000000000003634. Online ahead of print.

Reference Type: DERIVED

PMID: 40622440 (View on PubMed)

Other Identifiers

SAR

Identifier Type: -

Identifier Source: org_study_id