Trial Outcomes & Findings for NU-0129 in Treating Patients With Recurrent Glioblastoma or Gliosarcoma Undergoing Surgery (NCT NCT03020017)

NCT ID: NCT03020017

Last Updated: 2022-08-26

Results Overview

To evaluate the safety of intravenous NU-0129 in patients with recurrent GBM or GS, the number of adverse events will be assessed and will be graded according to the NCI's Common Terminology Criteria in Adverse Events (CTCAE) version 4.03 where the grading is as follows: Grade 1: Mild Grade 2: Moderate Grade 3: Severe Grade 4: Life-threatening Grade 5: Fatal

Recruitment status

COMPLETED

Study phase

EARLY_PHASE1

Target enrollment

8 participants

Primary outcome timeframe

Up to 21 days after study drug administration

Results posted on

2022-08-26

Participant Flow

Participant milestones

Participant milestones
Measure
NU-0129 Treatment
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
NU-0129
STARTED
8
NU-0129
COMPLETED
8
NU-0129
NOT COMPLETED
0
Standard of Care Resection
STARTED
8
Standard of Care Resection
COMPLETED
8
Standard of Care Resection
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

NU-0129 in Treating Patients With Recurrent Glioblastoma or Gliosarcoma Undergoing Surgery

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
NU-0129 Treatment
n=8 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Age, Continuous
55.5 years
n=5 Participants
Sex: Female, Male
Female
4 Participants
n=5 Participants
Sex: Female, Male
Male
4 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
8 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
1 Participants
n=5 Participants
Race (NIH/OMB)
White
7 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Region of Enrollment
United States
8 participants
n=5 Participants

PRIMARY outcome

Timeframe: Up to 21 days after study drug administration

To evaluate the safety of intravenous NU-0129 in patients with recurrent GBM or GS, the number of adverse events will be assessed and will be graded according to the NCI's Common Terminology Criteria in Adverse Events (CTCAE) version 4.03 where the grading is as follows: Grade 1: Mild Grade 2: Moderate Grade 3: Severe Grade 4: Life-threatening Grade 5: Fatal

Outcome measures

Outcome measures
Measure
NU-0129 Treatment
n=8 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Number of Patients With Adverse Events
Experienced any AE
8 Participants
Number of Patients With Adverse Events
Experienced an AE related to NU-01289
4 Participants
Number of Patients With Adverse Events
Experienced any SAE
1 Participants
Number of Patients With Adverse Events
Experienced an SAE related to NU-0129
0 Participants

SECONDARY outcome

Timeframe: At 1, 3, 5, 10, 30, and 60 minutes, and 4, 8, and 24 hours post infusion

Blood samples will be collected post-infusion to analyze drug concentration at specific time points after drug administration. Median plasma concentrations of NU-0129 were derived from time profiles for both Seven different small interfering RNA (siRNA) and gold (Au) concentrations, with Au plasma concentration determined by inductively coupled plasma mass spectrometry (ICP-MS) and siRNA concentration assessed by liquid chromatography-high performance liquid chromatography (LC-HPLC) using an atto dye-labeled PNA probe.

Outcome measures

Outcome measures
Measure
NU-0129 Treatment
n=8 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
NU-0129 Concentration in Blood After Drug Administration Using Maximum Concentration
Au maximum observed plasma concentration
4290 ng/ml
Interval 3120.0 to 7140.0
NU-0129 Concentration in Blood After Drug Administration Using Maximum Concentration
siRNA maximum observed plasma concentration
62.1 ng/ml
Interval 22.8 to 123.0

SECONDARY outcome

Timeframe: At time of surgery

Population: 2 patients tumor resections resulted in insufficient amount of viable tumor tissue for ICP-MS analysis and were not included in this analysis

Tissue will be collected during the scheduled surgery and assayed with Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to analyze the concentration of particles in various parts of tumor tissue. To analyze spatial distribution of Au within tumor tissue, synchrotron XFM elemental maps of GBM tissue slices were acquired at micron and submicron resolution and matched to adjacent hematoxylin and eosin (H\&E)- and Ki67-stained tumor sections. Approximate percentage of gold (Au) found in cancer cells is reported below.

Outcome measures

Outcome measures
Measure
NU-0129 Treatment
n=6 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Biodistribution of NU-0129 in Tumor Tissue
20.5 percentage of gold "Au"
Standard Deviation 8.15

SECONDARY outcome

Timeframe: At time of infusion (8-48 hours prior to resection) and during surgery

Population: Patients who had drug infused and underwent subsequent resection

Feasibility will be calculated as the rate of successful production, delivery, and administration of the investigational product and subsequent resection.

Outcome measures

Outcome measures
Measure
NU-0129 Treatment
n=8 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Feasibility of Giving NU-0129 as a Standard Treatment
Number of patients who had drug infused successfully
8 Participants
Feasibility of Giving NU-0129 as a Standard Treatment
Number of patient who underwent subsequent resection
8 Participants

SECONDARY outcome

Timeframe: At 1, 3, 5, 10, 30, and 60 minutes, and 4, 8, and 24 hours post infusion

Blood samples will be collected post-infusion to analyze drug concentration at specific time points after drug administration. Median plasma concentrations of NU-0129 were derived from time profiles for both Seven different small interfering RNA (siRNA) and gold (Au) concentrations, with Au plasma concentration determined by inductively coupled plasma mass spectrometry (ICP-MS) and siRNA concentration assessed by liquid chromatography-high performance liquid chromatography (LC-HPLC) using an atto dye-labeled PNA probe.

Outcome measures

Outcome measures
Measure
NU-0129 Treatment
n=8 Participants
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
NU-0129 Concentration in Blood After Drug Administration Using Half-life
siRNA half-life
0.06 hours
Interval 0.02 to 0.16
NU-0129 Concentration in Blood After Drug Administration Using Half-life
Au half-life
18 hours
Interval 12.0 to 23.0

Adverse Events

NU-0129 Treatment

Serious events: 1 serious events
Other events: 8 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
NU-0129 Treatment
n=8 participants at risk
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Nervous system disorders
Cerebrospinal fluid leakage
12.5%
1/8 • Number of events 1 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion

Other adverse events

Other adverse events
Measure
NU-0129 Treatment
n=8 participants at risk
NU-0129 will be administered inpatient at \~ 0.04mg/kg IV 8-48 hours once prior to scheduled tumor resection. Dose corresponds to 1/50th of the no-observed-adverse-event level.
Investigations
Alkaline phosphatase increased
12.5%
1/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion
Investigations
Lymphocyte count decreased
25.0%
2/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion
Investigations
Platelet count decreased
12.5%
1/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion
Investigations
White blood cell decreased
12.5%
1/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion
Metabolism and nutrition disorders
Hypophosphatemia
25.0%
2/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion
Vascular disorders
Hypertension
12.5%
1/8 • Patients were monitored for adverse events from the time of NU-0129 infusion through 21 days post infusion

Additional Information

Priya Kumthekar

Northwestern University

Phone: 312 908 5073

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place