Volumetric Study of Fat Resorption After Breast Lipofilling

NCT ID: NCT03016663

Last Updated: 2017-01-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

10 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-09-30

Study Completion Date

2016-12-31

Brief Summary

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Prospective observational study to evaluate effects on washing to improve viability of fat grafts in breast lipofilling.

Detailed Description

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Conditions

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Cosmetic Techniques Mammaplasty

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Breast Lipofilling Technique

Preliminary MRI breast were performed for volumetric measurement. Fat harvesting performed by same surgeon using water-assisted liposuction (WAL) and subsequent washing with buffered lactate in a standardized technique. Fat transfer performed using Berlin autologous lipotransplantation technique according to the BEAULI protocol .Patient were followed up monthly and MRI breast were repeated at 1st and 6th months after lipofilling. Clinical assessment and MRI Volumetric measurement performed using OsiriX (v7.0.3, 64 bit, Pixmeo c) software by same radiologist based on predefined operational procedure

Technique of Breast Lipofilling

Intervention Type PROCEDURE

1. Identification of the donor site Procedure carried out in single sessions under GA. Abdominal site identified and outlined with a skin marker.
2. Fat Harvesting Berlin autologous lipotransplantation technique used according to the BEAULI protocol described by Ueberreiter et al.

Infiltration solution -"Klein'solution" contains 1 cc of epinephrine (1:500,000) diluted in 500 cc of 0.001% lactate ringer solution (LRS).
3. Fat Processing After aspiration of nonviable components of the aspirate, fat graft washed using Lactate Ringer Solution for three times. The fat processing and washing were performed by the same assistant for all patients.
4. Fat Transfer (Injection) Fat is injected in small amounts as the needle is gradually withdrawn. Periglandular planes and intrapectoral planes are used. Intraparenchymal injection avoided.

Interventions

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Technique of Breast Lipofilling

1. Identification of the donor site Procedure carried out in single sessions under GA. Abdominal site identified and outlined with a skin marker.
2. Fat Harvesting Berlin autologous lipotransplantation technique used according to the BEAULI protocol described by Ueberreiter et al.

Infiltration solution -"Klein'solution" contains 1 cc of epinephrine (1:500,000) diluted in 500 cc of 0.001% lactate ringer solution (LRS).
3. Fat Processing After aspiration of nonviable components of the aspirate, fat graft washed using Lactate Ringer Solution for three times. The fat processing and washing were performed by the same assistant for all patients.
4. Fat Transfer (Injection) Fat is injected in small amounts as the needle is gradually withdrawn. Periglandular planes and intrapectoral planes are used. Intraparenchymal injection avoided.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Patients with breast defect post breast surgery and unsatisfied. The first lipofilling procedure delayed for at least 9 months after the completion of radiotherapy. Patient on chemotherapy deferred until at least 6 month of last chemotherapy.

Exclusion Criteria

* Patients are excluded if they experienced local recurrence or distant metastasis
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University of Malaya

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Alizan ABDUL KHALIL

Role: PRINCIPAL_INVESTIGATOR

Department of Surgery University Malaya Medical Centre

Locations

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University Malaya Medical Centre

Lembah Pantai, Kuala Lumpur, Malaysia

Site Status

Countries

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Malaysia

References

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Fiaschetti V, Pistolese CA, Fornari M, Liberto V, Cama V, Gentile P, Floris M, Floris R, Cervelli V, Simonetti G. Magnetic resonance imaging and ultrasound evaluation after breast autologous fat grafting combined with platelet-rich plasma. Plast Reconstr Surg. 2013 Oct;132(4):498e-509e. doi: 10.1097/PRS.0b013e3182a00e57.

Reference Type BACKGROUND
PMID: 24076696 (View on PubMed)

Agha RA, Goodacre T, Orgill DP. Use of autologous fat grafting for reconstruction postmastectomy and breast conserving surgery: a systematic review protocol. BMJ Open. 2013 Oct 22;3(10):e003709. doi: 10.1136/bmjopen-2013-003709.

Reference Type BACKGROUND
PMID: 24154518 (View on PubMed)

Petit JY, Lohsiriwat V, Clough KB, Sarfati I, Ihrai T, Rietjens M, Veronesi P, Rossetto F, Scevola A, Delay E. The oncologic outcome and immediate surgical complications of lipofilling in breast cancer patients: a multicenter study--Milan-Paris-Lyon experience of 646 lipofilling procedures. Plast Reconstr Surg. 2011 Aug;128(2):341-346. doi: 10.1097/PRS.0b013e31821e713c.

Reference Type BACKGROUND
PMID: 21502905 (View on PubMed)

Claro F Jr, Figueiredo JC, Zampar AG, Pinto-Neto AM. Applicability and safety of autologous fat for reconstruction of the breast. Br J Surg. 2012 Jun;99(6):768-80. doi: 10.1002/bjs.8722. Epub 2012 Apr 4.

Reference Type BACKGROUND
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Sasaki GH. Water-assisted liposuction for body contouring and lipoharvesting: safety and efficacy in 41 consecutive patients. Aesthet Surg J. 2011 Jan;31(1):76-88. doi: 10.1177/1090820X10391465.

Reference Type BACKGROUND
PMID: 21239675 (View on PubMed)

Ueberreiter K, von Finckenstein JG, Cromme F, Herold C, Tanzella U, Vogt PM. [BEAULI--a new and easy method for large-volume fat grafts]. Handchir Mikrochir Plast Chir. 2010 Dec;42(6):379-85. doi: 10.1055/s-0030-1267913. Epub 2010 Dec 15. German.

Reference Type BACKGROUND
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Crawford JL, Hubbard BA, Colbert SH, Puckett CL. Fine tuning lipoaspirate viability for fat grafting. Plast Reconstr Surg. 2010 Oct;126(4):1342-1348. doi: 10.1097/PRS.0b013e3181ea44a9.

Reference Type BACKGROUND
PMID: 20885257 (View on PubMed)

Herold C, Knobloch K, Grimme M, Vogt PM. Does the injection plane matter in autologous fat transplantation? Aesthetic Plast Surg. 2010 Oct;34(5):678-9. doi: 10.1007/s00266-010-9490-1. No abstract available.

Reference Type BACKGROUND
PMID: 20333518 (View on PubMed)

Coleman SR. Structural fat grafting: more than a permanent filler. Plast Reconstr Surg. 2006 Sep;118(3 Suppl):108S-120S. doi: 10.1097/01.prs.0000234610.81672.e7.

Reference Type BACKGROUND
PMID: 16936550 (View on PubMed)

Kanchwala SK, Glatt BS, Conant EF, Bucky LP. Autologous fat grafting to the reconstructed breast: the management of acquired contour deformities. Plast Reconstr Surg. 2009 Aug;124(2):409-418. doi: 10.1097/PRS.0b013e3181aeeadd.

Reference Type BACKGROUND
PMID: 19644255 (View on PubMed)

Hamza A, Lohsiriwat V, Rietjens M. Lipofilling in breast cancer surgery. Gland Surg. 2013 Feb;2(1):7-14. doi: 10.3978/j.issn.2227-684X.2013.02.03.

Reference Type BACKGROUND
PMID: 25083450 (View on PubMed)

Saint-Cyr M, Rojas K, Colohan S, Brown S. The role of fat grafting in reconstructive and cosmetic breast surgery: a review of the literature. J Reconstr Microsurg. 2012 Feb;28(2):99-110. doi: 10.1055/s-0031-1287675. Epub 2011 Sep 16.

Reference Type BACKGROUND
PMID: 21928171 (View on PubMed)

Losken A, Pinell XA, Sikoro K, Yezhelyev MV, Anderson E, Carlson GW. Autologous fat grafting in secondary breast reconstruction. Ann Plast Surg. 2011 May;66(5):518-22. doi: 10.1097/SAP.0b013e3181fe9334.

Reference Type BACKGROUND
PMID: 21451387 (View on PubMed)

ELFadl D, Garimella V, Mahapatra TK, McManus PL, Drew PJ. Lipomodelling of the breast: a review. Breast. 2010 Jun;19(3):202-9. doi: 10.1016/j.breast.2010.02.009. Epub 2010 Mar 26.

Reference Type BACKGROUND
PMID: 20347309 (View on PubMed)

Costantini M, Cipriani A, Belli P, Bufi E, Fubelli R, Visconti G, Salgarello M, Bonomo L. Radiological findings in mammary autologous fat injections: a multi-technique evaluation. Clin Radiol. 2013 Jan;68(1):27-33. doi: 10.1016/j.crad.2012.05.009. Epub 2012 Jun 30.

Reference Type BACKGROUND
PMID: 22749812 (View on PubMed)

Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg. 2001 Sep;27(9):819-26. doi: 10.1046/j.1524-4725.2001.01062.x.

Reference Type BACKGROUND
PMID: 11553171 (View on PubMed)

Rennekampff HO, Reimers K, Gabka CJ, Germann G, Giunta RE, Knobloch K, Machens HG, Pallua N, Ueberreiter K, Heimburg Dv, Vogt PM. [Current perspective and limitations of autologous fat transplantation--"consensus meeting" of the German Society of Plastic, Reconstructive and Aesthetic Surgeons at Hannover; September 2009]. Handchir Mikrochir Plast Chir. 2010 Apr;42(2):137-42. doi: 10.1055/s-0030-1249672. Epub 2010 Mar 29. German.

Reference Type BACKGROUND
PMID: 20352577 (View on PubMed)

Spear SL, Wilson HB, Lockwood MD. Fat injection to correct contour deformities in the reconstructed breast. Plast Reconstr Surg. 2005 Oct;116(5):1300-5. doi: 10.1097/01.prs.0000181509.67319.cf.

Reference Type BACKGROUND
PMID: 16217471 (View on PubMed)

Smith P, Adams WP Jr, Lipschitz AH, Chau B, Sorokin E, Rohrich RJ, Brown SA. Autologous human fat grafting: effect of harvesting and preparation techniques on adipocyte graft survival. Plast Reconstr Surg. 2006 May;117(6):1836-44. doi: 10.1097/01.prs.0000218825.77014.78.

Reference Type BACKGROUND
PMID: 16651956 (View on PubMed)

Schultz I, Lindegren A, Wickman M. Improved shape and consistency after lipofilling of the breast: patients' evaluation of the outcome. J Plast Surg Hand Surg. 2012 Apr;46(2):85-90. doi: 10.3109/2000656X.2011.653256.

Reference Type BACKGROUND
PMID: 22471255 (View on PubMed)

Conde-Green A, de Amorim NF, Pitanguy I. Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: a comparative study. J Plast Reconstr Aesthet Surg. 2010 Aug;63(8):1375-81. doi: 10.1016/j.bjps.2009.07.018. Epub 2009 Aug 12.

Reference Type BACKGROUND
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Hsu VM, Stransky CA, Bucky LP, Percec I. Fat grafting's past, present, and future: why adipose tissue is emerging as a critical link to the advancement of regenerative medicine. Aesthet Surg J. 2012 Sep;32(7):892-9. doi: 10.1177/1090820X12455658.

Reference Type BACKGROUND
PMID: 22942117 (View on PubMed)

Mestak O, Hromadkova V, Fajfrova M, Molitor M, Mestak J. Evaluation of Oncological Safety of Fat Grafting After Breast-Conserving Therapy: A Prospective Study. Ann Surg Oncol. 2016 Mar;23(3):776-81. doi: 10.1245/s10434-015-4908-2. Epub 2015 Oct 14.

Reference Type BACKGROUND
PMID: 26467459 (View on PubMed)

Uda H, Sugawara Y, Sarukawa S, Sunaga A. Brava and autologous fat grafting for breast reconstruction after cancer surgery. Plast Reconstr Surg. 2014 Feb;133(2):203-213. doi: 10.1097/01.prs.0000437256.78327.12.

Reference Type BACKGROUND
PMID: 24150122 (View on PubMed)

Other Identifiers

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RP046D-15HTM

Identifier Type: -

Identifier Source: org_study_id

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