Study of Adoptive Immunotherapy With Donor-derived CMV-specific T Cells for Recipients of Allo-HSCT

NCT ID: NCT02988258

Last Updated: 2018-09-12

Basic Information

• Recruitment Status: SUSPENDED
• Clinical Phase: PHASE1
• Total Enrollment: 10 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2013-07-31
• Study Completion Date: 2019-08-31

Brief Summary

The study will test the hypothesis that CMV TCR-transduced T cells, at a specific T-cell dose/kg, can generate a functional CMV immune response post-transplant, where CMV-specific donor T cells cannot be isolated by conventional means. This will be tested in the context of adult HLA-matched sibling allogeneic HSCT.

In the proposed trial, an HLA-A*0201-restricted CMV pp65-specific T cell receptor (TCR) will be introduced into donor T cells via ex vivo GMP retroviral transduction. Donor T cells will be isolated from peripheral blood following a simple venesection procedure. The CMV TCR-transduced T cells will be tested for TCR expression, CMV-specific cytokine secretion and microbiological contamination before being frozen and stored at -80C. CMV seropositive transplant recipients will be tested weekly for CMV reactivation by quantitative PCR on peripheral blood. On first detection of CMV DNA > 200 copies/ml, 104 (cohort 1) or 105 (cohort 2) bulk CMV TCR-transduced T cells/kg recipient weight will be infused into the patient.

Blood will be taken regularly to determine persistence and expansion of the CMV TCR-transduced T cells. Weekly CMV PCR will be continued. Patients will be examined at appropriate intervals (daily if inpatients, twice weekly in BMT clinic if outpatients) for the development of graft versus host disease (GVHD) or other potential side effects.

Detailed Description

Reactivation of the latent human herpes virus, Cytomegalovirus (CMV), post allogeneic haematopoietic stem cell transplantation (Allo-HSCT) can result in significant morbidity and mortality unless treated promptly. Anti-viral therapy is usually effective, but has serious side effects typically requiring prolonged inpatient admission, such as myelosuppression (Ganciclovir) or nephrotoxicity (Foscarnet). Cellular immunotherapy for CMV has been tested in Phase I/II trials in the UK and Europe. In these trials CMV-specific T cells were isolated from the peripheral blood of CMV seropositive donors and re-infused into recipients following CMV reactivation resulting in sustained anti-viral responses. It is clear that post-transplant recovery of CD8+ CMV-specific cytotoxic T-cells (CTL) abrogates the development of CMV-related disease. An advantage of cellular therapy for CMV reactivation is the transfer of immunological memory, which can reduce the number of subsequent reactivations. This is important, as rapidly increasing numbers of highly immunosuppressive (or T cell depleted) reduced intensity conditioning Allo-HSCTs are being performed in the UK. These approaches reduce the toxicity of transplantation in older patients with more co-morbidities. Reduced intensity allogeneic transplants are currently part of the UK MRC AML15 trial and are to be recommended in the proposed MRC/ECOG UKALL14 study and again in UK MRC AML17. In addition, a number of NCRN approved UK multicentre extended Phase II studies have recently started using reduced intensity Allo-HSCT in various lymphoma subtypes.

Transplant recipients with CMV seronegative donors cannot benefit from currently available cellular immunotherapy approaches due to the lack of CMV-specific memory T cells in these donors. At present, there is no reliable strategy to isolate virus specific T cells from uninfected naïve individuals, as the precursor frequency is low or absent and the in vitro priming of T-cell responses is inefficient. T-cell receptor (TCR) gene transfer offers a strategy to produce antigen-specific T cells independent of precursor frequency and without the need for T-cell priming. As approximately 50% of adult individuals have been previously infected with CMV, there are significant numbers of CMV 'mismatched' Allo-HSCT performed, where the donor is CMV seronegative and the recipient CMV seropositive. The proposed study will test the feasibility of generating donor-derived CMV-specific T cells via the ex vivo introduction of a CMV-specific T cell receptor using a GMP grade retroviral vector.

Conditions

Haematological Malignancies; CMV Infection

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Cohort 1 - 10^4 transduced cells/kg

The first 3 patients will receive a single infusion of bulk CMV-TCR transduced donor-derived T cells on first CMV reactivation post allogeneic HSCT, at a dose of 10\^4 T cells/kg recipient weight

Group Type: EXPERIMENTAL

CMV-TCR transduced donor-derived T cells

Intervention Type: GENETIC

Dose escalation

Cohort 2 - 10^5 transduced cells/kg

If no cases grade III-IV GVHD in Cohort 1 the remaining patients (N=7) each receive a single infusion of bulk CMV-TCR transduced donor-derived T cells on first CMV reactivation post allogeneic HSCT, at a dose of 10\^5 T cells/kg recipient weight

Group Type: EXPERIMENTAL

CMV-TCR transduced donor-derived T cells

Intervention Type: GENETIC

Dose escalation

Cohort 1a - 10^3 transduced cells/kg

If 1 case grade III-IV GVHD in Cohort 1, next three patients to be treated with a single infusion of bulk CMV-TCR transduced donor-derived T cells of 1 x 10\^3 T cells/kg recipient weight

Group Type: EXPERIMENTAL

CMV-TCR transduced donor-derived T cells

Intervention Type: GENETIC

Dose escalation

Interventions

CMV-TCR transduced donor-derived T cells

Dose escalation

Intervention Type: GENETIC

Eligibility Criteria

Inclusion Criteria

• Undergoing matched sibling allogeneic HSCT for an underlying haematological malignancy with a CMV seronegative donor
• Age ≥ 18 years and ≤ 65 years
• HLA-A*0201 positive
• CMV seropositive (CMV IgG detected) pre-transplant
• Informed consent in writing and ability to co-operate with treatment and follow up.

• Prepared to undergo additional study procedures as per study schedule
• Patient has undergone counselling about risk
• Serologically negative for HIV 1&2, Hep B, Hep C and syphilis
• Female patients of child-bearing age must have a negative pregnancy test and agree to use reliable contraceptive methods for the duration of the therapy and for 6 months afterwards
• Male patients must agree to use appropriate medically approved contraception during the trial and for six months afterwards

And to be assessed prior to CMV-specific T cell infusion (for confirmation prior to product release):

• Donor engraftment (neutrophils > 0.5x109/l).
• Single positive CMV PCR result (> 200 copies/ml)

Exclusion Criteria

• Pregnant or lactating women
• Co-existing medical problems that would place the patient at significant risk of death due to GVHD or its sequelae
• HIV infection

And to be assessed prior to CMV-specific T cell infusion (for confirmation prior to product release):

• Active acute GVHD > Grade I
• Concurrent use of systemic corticosteroids
• Organ dysfunction as measured by

• creatinine > 200 uM/l
• bilirubin > 50 uM/l
• ALT > 3x upper limit of normal

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

University College, London

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Emma Morris, Prof

Role: PRINCIPAL_INVESTIGATOR

University College, London

Hans Stauss, Prof

Role: STUDY_CHAIR

University College, London

Locations

University Hospitals Birmingham NHS Foundation Trust

Birmingham, , United Kingdom

University Hospitals Bristol NHS Foundation Trust

Bristol, , United Kingdom

University College London Hospital

London, , United Kingdom

Nottingham University Hospital

Nottingham, , United Kingdom

Countries

United Kingdom

References

Tendeiro Rego R, Morris EC, Lowdell MW. T-cell receptor gene-modified cells: past promises, present methodologies and future challenges. Cytotherapy. 2019 Mar;21(3):341-357. doi: 10.1016/j.jcyt.2018.12.002. Epub 2019 Jan 14.

Reference Type: DERIVED

PMID: 30655164 (View on PubMed)

Other Identifiers

2008-006649-18

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

G0701703

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

08/0214

Identifier Type: OTHER

Identifier Source: secondary_id

UCL 08/0214

Identifier Type: -

Identifier Source: org_study_id