Bioavailability of Red Wine Anthocyanins

NCT ID: NCT02975856

Last Updated: 2016-11-29

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE1
• Total Enrollment: 6 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2015-12-31
• Study Completion Date: 2016-07-31

Brief Summary

The main aim of this study is to evaluate the bioavailability of table red wine and young Port red wine anthocyanins in healthy men.

Detailed Description

Anthocyanins (ANT) are water soluble pigments found in plants, flowers and fruits that are consumed as part of the human diet, many times in higher amounts than other flavonoid classes. Red wine is also an important source of anthocyanins, especially Mv3glc.

Several epidemiological studies have suggested that the consumption of anthocyanin-rich foods, including the moderate consumption of red wine, is positively associated with the prevention of cardiovascular heart disease.

It has become clear that the flavonoid bioactive forms in vivo are not necessarily those which occur in nature, but metabolites arising after absorption takes place.Therefore, studying the bioavailability of red wine ANT is very important to identify which metabolites (originated in vivo) can actually reach the target organs (and in which concentrations) and may be responsible for the postulated health benefits of red wine.

Intervention protocol:

Urine samples and peripheral venous blood (10 ml) will be collected from 10 h-fasting subjects. Afterwards, each volunteer will consume 250 ml of table red wine or 150 ml of young Port red wine and blood samples will be collected 15, 30, 60 and 120 min after wines ingestion. Another urine sample will be collected at 120 min.

Table red wine 12 % (250 ml) and young Port red wine 20 % (150 ml), two different food matrices rich in ANT, provided the same amount of ethanol (24 g of ethanol).

A thorough screening analysis for ANT will be performed in plasma and urine samples collected from the volunteers at different time points.

Conditions

Healthy

Keywords

Anthocyanins; Bioavailability; Flavonoids; Port wine; Red wine

Study Design

• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Table Red Wine

250 ml Table Red Wine (12% ethanol)

Group Type: EXPERIMENTAL

Table Red Wine

Intervention Type: DIETARY_SUPPLEMENT

Young Port Red Wine

150 ml Young Port Red Wine (20% ethanol)

Group Type: EXPERIMENTAL

Young Port Red Wine

Intervention Type: DIETARY_SUPPLEMENT

Interventions

Table Red Wine

Intervention Type: DIETARY_SUPPLEMENT

Young Port Red Wine

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• Men
• Age 18-65 years
• BMI < 25 kg/m2
• Willing and able to provide written consent

Exclusion Criteria

• Subjects with current or previous CVD, diabetes or other severe chronic disease
• Subjects under prescription of any chronic medication
• Intake of red fruits or red wine in the last 24 h prior to the beginning of the intervention
• Subjects involved in any clinical or food study within the preceding month

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 45 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

REQUIMTE/LAQV, Departamento de Química e Bioquímica, FCUP

UNKNOWN

Sponsor Role: collaborator

Center for Health Technology and Services Research

OTHER

Sponsor Role: collaborator

Vinoflavo

INDUSTRY

Sponsor Role: collaborator

Universidade do Porto

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Conceição Calhau, PhD

Role: PRINCIPAL_INVESTIGATOR

CINTESIS - Faculty of Medicine of University of Porto

Locations

CINTESIS - Faculty of Medicine of the University of Porto

Porto, , Portugal

Countries

Portugal

Other Identifiers

VINOFLAVO_02

Identifier Type: -

Identifier Source: org_study_id