TSK (Tryptophan - Serotonin - Kynurenine) Biomarkers Assessment in Stroke

NCT ID: NCT02963545

Last Updated: 2016-11-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

28 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-10-31

Study Completion Date

2016-05-31

Brief Summary

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Single-center, prospective, descriptive and biomedical research with controls, without health product.

Depression is the second risk factor for stroke as tobacco smoking following hypertension. Peripheral abnormalities in serotonin parameters were described in depression and tobacco smoking. The investigators hypothesized dysregulations in pathways of serotonin (5-HT), which has notably complex vasomotor effects and of kynurenine which could have cognitive dysfunction effects.

The aim of this study is to evaluate simultaneously the involvement of serotonin and kynurenine pathways parameters in patients suffering from a cerebral infarction shortly after the onset (less than 4 hours and a half), within a 2 days follow-up (Day 1 and Day 2) and 3 months after the cerebral infarction.

Detailed Description

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Tryptophan (TRP), an essential aminoacid, is metabolized in the serotonin (5-HT) or in the kynurenine (KYN) pathway. Serotonin is catabolized to 5-HIAA (5-hydroxyindole amino acid) by monoamine oxidase A (MAOA). The TRP to KYN transformation is regulated by indoleamine 2,3-dioxygenase (IDO).

The primary objective was the measurements of serotonin and kynurenine pathways parameters which were performed in blood and urine samples using different HPLC techniques and of serotonin transporters and receptors which were determined in blood platelets. The results in patients were compared to those measured in controls matched for age, gender, tobacco consumption and season of inclusion.

The secondary objective was to evaluate the potential relationships between these 5-HT and Kyn pathways biomarkers concentrations, MAOA and IDO enzymatic activations and clinical outcome and criteria.

Conditions

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Cerebral Infarction

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

NONE

Study Groups

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Patients presenting cerebral infarction

no intervention of health product administration,

Group Type EXPERIMENTAL

Usual care patients in neurology department

Intervention Type OTHER

patients characteristics, history, clinical signs chronology and usual medical care by the emergency units, cerebral infarction area

blood and urines sampling

Intervention Type BIOLOGICAL

Collection samples for biochemical determinations of serotonin pathway and kynurenine pathway parameters determinations

Psychiatric evaluation

Intervention Type PROCEDURE

depression scale, impulsivity scale, hostility scale , tobacco consumption questioning

Historical controls

no intervention of health product administration, patients characteristics, history, matched with patients for age, gender, tobacco consumption and season of inclusion, free of neurologic or psychiatric disease or psychotropic medications or medications known to impact on serotonin

Group Type OTHER

blood and urines sampling

Intervention Type BIOLOGICAL

Collection samples for biochemical determinations of serotonin pathway and kynurenine pathway parameters determinations

Interventions

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Usual care patients in neurology department

patients characteristics, history, clinical signs chronology and usual medical care by the emergency units, cerebral infarction area

Intervention Type OTHER

blood and urines sampling

Collection samples for biochemical determinations of serotonin pathway and kynurenine pathway parameters determinations

Intervention Type BIOLOGICAL

Psychiatric evaluation

depression scale, impulsivity scale, hostility scale , tobacco consumption questioning

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Age ≥ 18 ans
* Cerebral infarction with a medical care to emergencies less than 4.30 hours after the symptoms onset.
* Written informed consent signed by the patient, or by a trusted person then by the patient himself if permitted by his condition.

Exclusion Criteria

* Cerebral infarction with a medical care to emergencies more than 4.30 hours after the symptoms onset.
* Patient with subarachnoid haemorrhage, cerebral hematoma.
* Pregnant woman
* Patient under guardianship or trusteeship, or safeguard justice.

Control group :

* Matching criteria for age, gender, tobacco smoking, inclusion season
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Versailles Hospital

OTHER

Sponsor Role lead

Responsible Party

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Odile VAROQUAUX

Investigator coordinator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Odile SPREUX-VAROQUAUX, PhD

Role: PRINCIPAL_INVESTIGATOR

Pharmacology, Versailles Hospital and Versailles University

Fernando PICO, Neurology Department head

Role: STUDY_DIRECTOR

Versailles Hospital

Locations

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Centre Hospitalier de Versailles

Le Chesnay, , France

Site Status

Countries

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France

Other Identifiers

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P11/25_AVC-TSK

Identifier Type: -

Identifier Source: org_study_id