Precision Diagnosis for Intraoperative Frozen Section of Early Stage Lung Cancer

NCT ID: NCT02941003

Last Updated: 2016-10-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

540 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-02-29

Study Completion Date

2019-10-31

Brief Summary

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The purpose of this study is:

1. To establish a set of diagnostic criteria of intraoperative frozen section of early stage lung adenocarcinoma, including clinicopathologic and molecular characteristics.
2. To assess its clinical usefulness in guiding surgical procedure for early stage lung adenocarcinoma.

Detailed Description

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The detection rate of small pulmonary nodules is increasing due to the widespread use of high-resolution computed tomography (CT) screening in clinical practice. These minute pulmonary nodules are suggestive of atypical adenomatous hyperplasia (AHH), adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and invasive adenocarcinoma. Hence, the precision diagnosis of intraoperative frozen section is particularly imperative for its fundamental role in assess indeterminate lung lesions and guide the extent of subsequent surgical procedure.

However, it is still difficult for the surgical pathologist to apply the various features according to the newly revised WHO classification of lung adenocarcinoma (2015) to guide surgical management due to their unascertained accuracy or heterogeneity of lung cancer or technique problem. Therefore, the establishment and assessment for a set of diagnostic criteria of intraoperative frozen section of early stage lung adenocarcinoma are our primary aim in this study.

Conditions

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Lung Cancer

Keywords

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Early stage lung adenocarcinoma Intraoperative frozen section Diagnostic criteria

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

FACTORIAL

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

SINGLE

Participants

Study Groups

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OCT treatment

Randomly assigned patients whose small pulmonary nodules are inflated with the diluted OCT medium (2:3) used for frozen section, and then detected under microscope for their histopathological characteristics, by immunostaining for specific protein expression, by NGS (next-generation sequencing) for driver mutations.

Group Type EXPERIMENTAL

microscope

Intervention Type DEVICE

Detection of of histopathological characteristics of OCT treatment or OCT free samples from patients .

immunostaining

Intervention Type OTHER

Detection of CD31/CD34, D2-40, Ki67 and p53 immunostaining of OCT treatment or OCT free samples from patients .

NGS

Intervention Type DEVICE

The investigators used the device to detect the gene mutations of OCT treatment or OCT free samples obtained from part of the patients.

OCT free

Randomly assigned patients whose small pulmonary nodules are uninflated with OCT used for frozen section, and then detected under microscope for their histopathological characteristics, by immunostaining for specific protein expression, by NGS for driver mutations.

Group Type EXPERIMENTAL

microscope

Intervention Type DEVICE

Detection of of histopathological characteristics of OCT treatment or OCT free samples from patients .

immunostaining

Intervention Type OTHER

Detection of CD31/CD34, D2-40, Ki67 and p53 immunostaining of OCT treatment or OCT free samples from patients .

NGS

Intervention Type DEVICE

The investigators used the device to detect the gene mutations of OCT treatment or OCT free samples obtained from part of the patients.

Interventions

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microscope

Detection of of histopathological characteristics of OCT treatment or OCT free samples from patients .

Intervention Type DEVICE

immunostaining

Detection of CD31/CD34, D2-40, Ki67 and p53 immunostaining of OCT treatment or OCT free samples from patients .

Intervention Type OTHER

NGS

The investigators used the device to detect the gene mutations of OCT treatment or OCT free samples obtained from part of the patients.

Intervention Type DEVICE

Other Intervention Names

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Illumina Nexseq500

Eligibility Criteria

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Inclusion Criteria

1. Patients with suspected pulmonary nodules (5\~20 mm) by chest CT scan;
2. Patients received surgical operation.

Exclusion Criteria

1. Patients compliance is poor and cannot accept follow-up;
2. Patients nursing or pregnant;
3. Patients with a history of any cancer;
4. Patients with confirmed pathological diagnosis or received radiotherapy or chemotherapy or target therapy preoperatively.
Minimum Eligible Age

40 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Shanghai Chest Hospital

OTHER

Sponsor Role lead

Responsible Party

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Jie Zhang

Chief Physician

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Jie Zhang, MD, MS.

Role: PRINCIPAL_INVESTIGATOR

Shanghai Chest Hospital

Locations

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Shanghai Chest Hospital

Shanghai, Shanghai Municipality, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Jie Zhang, MD, MS

Role: CONTACT

Phone: (+86)18017321572

Email: [email protected]

Keke Yu, MD, PhD

Role: CONTACT

Phone: (+86)18930859590

Email: [email protected]

Facility Contacts

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Jie Zhang, MD, MS

Role: primary

Keke Yu, MD, PhD

Role: backup

Other Identifiers

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Chest005

Identifier Type: -

Identifier Source: org_study_id