Clinical Response to Rhinovirus Challenge

NCT ID: NCT02910401

Last Updated: 2024-01-09

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 38 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2016-09-30
• Study Completion Date: 2022-09-21

Brief Summary

Rhinovirus (RV) infections represent the most common cause of asthma exacerbations in children and adolescents. The investigators hypothesize that the immune responses generated in the nose of allergic rhinitics and asthmatics underlie subsequent systemic modulation of the immune system, and that - in susceptible individuals (i.e., those with pre-existing asthma) - this modified nasal milieu is responsible for the asthma exacerbation.

Open label single center study in asthmatics as well as allergic rhinitis (AR) and healthy controls. All subjects will undergo good manufacturing practice (GMP) RV16 inoculation and responses will be compared between the 3 cohorts.

Detailed Description

Primary objectives are:

To determine whether RV increases expression of interleukin (IL)-25 transcripts by nasal epithelial cells in the asthma and AR but not control cohorts at the peak of infection (days 3 and 4).

To determine whether RV increases lower respiratory symptoms in the asthma but not AR and control cohorts.

To determine whether asthmatics and allergic rhinitics will demonstrate an increased severity of infection in comparison to control subjects.

Secondary objectives are:

1. To determine whether asthmatic and AR cohorts demonstrate increased IL-25 transcript expression over the course of RV infection

2. To determine whether asthmatic and AR cohorts demonstrate increased expression of mRNA transcripts of a type 2 cytokine-inducing profile (IL-33 and thymic stromal lymphopoietin (TSLP)).

3. To determine whether increased transcript expression of this type 2 cytokine-inducing profile can be corroborated as increased expression of protein.

4. To determine whether RV infection in the asthma cohort is associated with increases in biomarkers of inflammation.

5. To determine whether increased severity of RV infection in the asthma and AR cohorts will be associated with more symptoms.

6. To determine whether increased severity of RV infection in the asthma and AR cohorts is related to decreased innate immunity.

Conditions

Asthma; Allergic Rhinitis

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: HEALTH_SERVICES_RESEARCH
• Blinding Strategy: NONE

Study Groups

Asthmatic

Asthmatic subjects will be infected with Rhinovirus (GMP RV16 human (H)RV-16)

Group Type: ACTIVE_COMPARATOR

Rhinovirus (GMP RV16 HRV-16)

Intervention Type: BIOLOGICAL

300 tissue culture infectious dose (TCID)50 mg/ml intranasal one time only

Allergic rhinitis

Allergic rhinitis subjects will be infected with Rhinovirus (GMP RV16 HRV-16)

Group Type: ACTIVE_COMPARATOR

Rhinovirus (GMP RV16 HRV-16)

Intervention Type: BIOLOGICAL

300 tissue culture infectious dose (TCID)50 mg/ml intranasal one time only

Healthy control

Healthy controls will be infected with Rhinovirus (GMP RV16 HRV-16)

Group Type: ACTIVE_COMPARATOR

Rhinovirus (GMP RV16 HRV-16)

Intervention Type: BIOLOGICAL

300 tissue culture infectious dose (TCID)50 mg/ml intranasal one time only

Interventions

Rhinovirus (GMP RV16 HRV-16)

300 tissue culture infectious dose (TCID)50 mg/ml intranasal one time only

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

All subjects:

1. Subjects must be able to understand and provide written informed consent.

2. Age 18 to ≤40 years of age, any gender, any racial/ethnic origin

3. Female subjects of childbearing potential must have a negative pregnancy test upon study entry (day -7) and before each procedure involving pharmacologic interventions (days 0, 4, and 7).

4. Female (and male) subjects with reproductive potential, must agree to use FDA approved methods of birth control for the duration of the study such as, but not limited to, birth control pills, contraceptive foam, diaphragm, IUD, abstinence, or condoms.

5. Participants must be willing to comply with study procedures and requirements.

6. Negative test for serum neutralizing antibody to RV16 at enrollment visit (<1:8) (Visit 1).

Allergic Rhinitis Subjects:

1. Allergy as determined by ≥1 positive prick skin test (wheal ≥5 mm diameter and 3mm larger than the diluent control) to Virginia inhalant panel within 5 years, and a history of symptoms of sneezing, rhinorrhea, pruritus, nasal congestion, and/or allergic conjunctivitis on natural exposure to relevant allergens.

2. Negative methacholine challenge (less than 20% decline in functional expiratory volume in 1 second (FEV1) at ≤8mg/ml) within 1 year

3. FEV1 ≥80% predicted, FEV1/FVC ≥80%.

4. No history of wheezing with viral infection in the last 6 years, and no use of rescue inhalers or long-term controllers for asthma in the last 6 years.

Allergic Asthmatic Subjects:

1. Allergy as determined by ≥1 positive prick skin test (wheal ≥5 mm diameter and 3mm larger than the diluent control) to Virginia inhalant panel. Subjects are not required to have allergy symptoms at the time of study. Subjects will report history of symptoms of sneezing, rhinorrhea, pruritus, nasal congestion, and/or allergic conjunctivitis on natural exposure to relevant allergens.

2. Asthma determined by physician diagnosis and by a positive methacholine challenge (at least 20% fall in FEV1 at a methacholine concentration of ≤8 mg/ml) at screening protocol visit before enrollment (obtained within the past year).

3. Asthma must be controlled as determined by asthma control test (ACT) score ≥20 and normal lung function (FEV1>70% predicted or FEV1/FVC ratio >75% for subjects with FVC values between 80 and 87% predicted whose FEV1 values fall below 70%) at Visits 1 and 2.

Exclusion Criteria

1. Positive test for serum neutralizing antibody to RV16 at enrollment visit (≥1:8) (Visit 1).

2. Upper airway modified Jackson criteria symptom scores ≥7 at time of inoculation.

3. Chronic heart disease including bradycardia, lung diseases other than asthma, or other chronic illnesses including epilepsy, peptic ulcer disease, thyroid disease, urinary tract infection, vagotonia, autoimmune disease, primary or secondary immunodeficiency or any household contacts who are known to be immune deficient. Any medical conditions that could be adversely affected by the administration of cholinergic agent.

4. Any use of corticosteroids, leukotriene (LT) modifiers, antihistamines, omalizumab, theophylline, long-acting anti-muscarinic antagonists (LAMAs), long-acting beta-agonists (LABAs), nedocromil, cromolyn use on a daily basis within 4 weeks prior to Visit 1.

5. Current use of ß-blockers or cholinesterase inhibitors (for myasthenia gravis).

6. ß2-agonist use ≥4 days/week in any week or ≥2 nights/month during the month before Visit 1.

7. Recent (within 1-yr) asthma exacerbation requiring urgent care visit (unless the treatment involved only the use of a bronchodilator), hospitalization, or oral CCS

8. Intubation or management in the intensive care unit (ICU) for an asthma exacerbation ever.

9. An upper or lower respiratory tract infection within 2 months prior to enrollment.

10. Previous nasal or sinus surgery within the last 12 months

11. >5 pack-year smoking history or any smoking within the past 6 mos.

12. Hemoglobin <11.5 g/dL for non-African American subjects or hemoglobin < 11.0 g/dL for African American subjects detected at Visit 1.

13. Laboratory values (other than hemoglobin and absolute neutrophil count (ANC)) measured at Visit 1 that are considered to be of clinical relevance by the Investigator.

14. Absolute neutrophil count (ANC) <1500 cells/mm3 (or 1.5 K/µL) or absolute lymphocyte count (ALC) <800 cells/mm3 detected at Visit 1.

15. Use of investigational drugs within 12 weeks of participation

16. Past or current medical problems or findings from physical examination or laboratory testing that are not listed above, which, in the opinion of the investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or that may impact the quality or interpretation of the data obtained from the study.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

National Institutes of Health (NIH)

NIH

Sponsor Role: collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role: collaborator

University of Virginia

OTHER

Sponsor Role: lead

Responsible Party

Larry Borish, MD

Professor of Medicine and Microbiology

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Larry Borish, MD

Role: PRINCIPAL_INVESTIGATOR

University of Virginia

Locations

University of Virginia Health System

Charlottesville, Virginia, United States

Countries

United States

References

Feng X, Lawrence MG, Payne SC, Mattos J, Etter E, Negri JA, Murphy D, Kennedy JL, Steinke JW, Borish L. Lower viral loads in subjects with rhinovirus-challenged allergy despite reduced innate immunity. Ann Allergy Asthma Immunol. 2022 Apr;128(4):414-422.e2. doi: 10.1016/j.anai.2022.01.007. Epub 2022 Jan 12.

Reference Type: RESULT

PMID: 35031416 (View on PubMed)

Provided Documents

Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form

View Document

Other Identifiers

1U01AI123337

Identifier Type: NIH

Identifier Source: secondary_id

View Link

19157

Identifier Type: -

Identifier Source: org_study_id