Trial Outcomes & Findings for OTL38 Injection for Intraoperative Imaging of Folate Receptor Positive Lung Nodules (NCT NCT02872701)

NCT ID: NCT02872701

Last Updated: 2023-03-24

Results Overview

Sensitivity or True Positive Rate (TPR) for OTL38 in combination with fluorescent light, defined as the proportion of fluorescent light positive tissue samples (nodule, synchronous lesion, and margin but excluding lymph nodes) that are histologically confirmed to be FR+ and lung cancer by central pathology relative to the total number of tissue samples confirmed to be FR+ and lung cancer by central pathology. Sensitivity = (True Positive)/(True Positive +False Negative)

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

100 participants

Primary outcome timeframe

1 day

Results posted on

2023-03-24

Participant Flow

Participant milestones

Participant milestones
Measure
Patients Receiving OTL38
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Overall Study
STARTED
100
Overall Study
COMPLETED
97
Overall Study
NOT COMPLETED
3

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

OTL38 Injection for Intraoperative Imaging of Folate Receptor Positive Lung Nodules

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Patients Receiving OTL38
n=100 Participants
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Age, Categorical
<=18 years
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
28 Participants
n=5 Participants
Age, Categorical
>=65 years
72 Participants
n=5 Participants
Age, Continuous
67.4 years
STANDARD_DEVIATION 9.35 • n=5 Participants
Sex: Female, Male
Female
68 Participants
n=5 Participants
Sex: Female, Male
Male
32 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
1 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
98 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
1 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
1 Participants
n=5 Participants
Race (NIH/OMB)
Asian
7 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
6 Participants
n=5 Participants
Race (NIH/OMB)
White
86 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Region of Enrollment
Netherlands
10 Participants
n=5 Participants
Region of Enrollment
United States
90 Participants
n=5 Participants

PRIMARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

Sensitivity or True Positive Rate (TPR) for OTL38 in combination with fluorescent light, defined as the proportion of fluorescent light positive tissue samples (nodule, synchronous lesion, and margin but excluding lymph nodes) that are histologically confirmed to be FR+ and lung cancer by central pathology relative to the total number of tissue samples confirmed to be FR+ and lung cancer by central pathology. Sensitivity = (True Positive)/(True Positive +False Negative)

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=107 Lesions
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Sensitivity or True Positive Rate (TPR)
0.854 proportion of lesions
Interval 0.74 to 0.92

PRIMARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

False positive rate (FPR) for OTL38 in combination with fluorescent light, for the purpose of this protocol, will be calculated as 1 - the Positive Predictive Value (PPV) and is defined as the proportion of fluorescent light positive tissue samples removed (nodule, synchronous lesion, and margin but excluding lymph nodes) that are histologically confirmed to be non-cancerous, or if cancerous, not FR+ and lung cancer, by central pathology relative to the total number of tissue samples removed with fluorescent light imaging. False Positive Rate = (False Positives) / (True Positives + False Positives)

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=107 Lesions
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
False Positive Rate (FPR)
0.138 proportion of lesions
Interval 0.08 to 0.23

SECONDARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

Proportion of patients with at least 1 Clinically Significant Event (CSE as a result of utilizing OLT-38 and Near Infrared Imaging

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=75 Participants
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Proportion of Patients With at Least 1 Clinically Significant Event (CSE)
0.293 proportion of participants
Interval 0.194 to 0.41

SECONDARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

Number of patients where at least one positive margin (fluorescent cancerous cells within 5 mm of the staple line) is identified with only OTL-38 and NIR

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=75 Participants
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Positive Margin Identification
8 Participants

SECONDARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

Number of patients where at least one synchronous lesions is identified only with OTL-38 and NIR

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=75 Participants
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Synchronous Lesion Identification
7 Participants

SECONDARY outcome

Timeframe: 1 day

Population: The FAS for the analysis of the two co-primary endpoints, sensitivity and FPR, included patients exposed to OTL38 who: * Were evaluated under both normal light and fluorescent light imaging, and * Had central pathology and histology confirmation (positive for FR+ Lung Cancer) for at least one tissue sample detected under normal light or fluorescent light imaging

Number of pulmonary nodules identified with OTL-38 and Near Infrared Imaging (NIR) that could not otherwise be identified by white light and palpation.

Outcome measures

Outcome measures
Measure
Patients Receiving OTL38
n=75 Participants
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Pulmonary Nodule Identification
10 number of pulmonary nodules

Adverse Events

Patients Receiving OTL38

Serious events: 14 serious events
Other events: 94 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Patients Receiving OTL38
n=100 participants at risk
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Respiratory, thoracic and mediastinal disorders
Haemothorax
2.0%
2/100 • Number of events 2 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Pleural effusion
2.0%
2/100 • Number of events 2 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Pneumothorax
2.0%
2/100 • Number of events 2 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Chylothorax
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Lung disorder
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Pulmonary embolism
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Respiratory failure
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Infections and infestations
Pneumonia
2.0%
2/100 • Number of events 2 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Injury, poisoning and procedural complications
Hip fracture
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Injury, poisoning and procedural complications
Post procedural haemorrhage
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Gastrointestinal disorders
Small intestinal obstruction
1.0%
1/100 • Number of events 2 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Vascular disorders
Arterial haemorrhage
1.0%
1/100 • Number of events 1 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration

Other adverse events

Other adverse events
Measure
Patients Receiving OTL38
n=100 participants at risk
All patients in this arm will receive OTL38 for injection and undergo intraoperative imaging. OTL38 for Injection: Folate analog ligand conjugated with an indole cyanine-like green dye as a solution in vials containing 3 mL at 2 mg/mL Near infrared camera imaging system: Near infrared camera imaging system Endoscopic or Thoracic Surgery: Endoscopic or Thoracic Surgery
Injury, poisoning and procedural complications
Procedural pain
50.0%
50/100 • Number of events 50 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Injury, poisoning and procedural complications
Incision site pain
20.0%
20/100 • Number of events 20 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Cough
29.0%
29/100 • Number of events 29 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Dyspnoea
17.0%
17/100 • Number of events 17 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Pneumothorax
17.0%
17/100 • Number of events 17 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Pleural effusion
13.0%
13/100 • Number of events 13 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Wheezing
7.0%
7/100 • Number of events 7 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Hypoxia
6.0%
6/100 • Number of events 6 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
6.0%
6/100 • Number of events 6 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Gastrointestinal disorders
Nausea
33.0%
33/100 • Number of events 33 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Gastrointestinal disorders
Vomiting
17.0%
17/100 • Number of events 17 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Gastrointestinal disorders
Constipation
10.0%
10/100 • Number of events 10 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
General disorders
Chest pain
12.0%
12/100 • Number of events 12 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
General disorders
Fatigue
10.0%
10/100 • Number of events 10 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
General disorders
Pyrexia
6.0%
6/100 • Number of events 6 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
General disorders
Respiratory complication associated with device
6.0%
6/100 • Number of events 6 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Vascular disorders
Hypertension
11.0%
11/100 • Number of events 11 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Vascular disorders
Hypotension
11.0%
11/100 • Number of events 11 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Cardiac disorders
Atrial fibrillation
8.0%
8/100 • Number of events 8 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Cardiac disorders
Tachycardia
5.0%
5/100 • Number of events 5 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Nervous system disorders
Dizziness
7.0%
7/100 • Number of events 7 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Musculoskeletal and connective tissue disorders
Muscle spasms
6.0%
6/100 • Number of events 6 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Infections and infestations
Pneumonia
7.0%
7/100 • Number of events 7 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Skin and subcutaneous tissue disorders
Pruritus
5.0%
5/100 • Number of events 5 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Skin and subcutaneous tissue disorders
Subcutaneous emphysema
5.0%
5/100 • Number of events 5 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Metabolism and nutrition disorders
Hypokalaemia
5.0%
5/100 • Number of events 5 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration
Renal and urinary disorders
Urinary retention
5.0%
5/100 • Number of events 5 • AEs were collected through Day 28 (+/- 4 days) from OTL38 administration

Additional Information

Tim Biro

On Target Laboratories

Phone: 216-533-3082

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place

Restriction type: LTE60