Trial Outcomes & Findings for Identification and Characterization of Bacteria in the Lungs of Children From 6 Months up to 6 Years Old, With Suspected Infection of the Lungs, in Spain. (NCT NCT02838407)

NCT ID: NCT02838407

Last Updated: 2019-10-04

Results Overview

Bacterial aetiology was assessed by culture growth from BAL fluid sample for Steptococcus pneumoniae (S.p.), Haemophilus influenzae (H.i.) and Moraxella catarrhalis (M.c.) , through either qualitative or quantitative bacterial identification (B.I.). The categories assessed were: positive (Pos.), negative (Neg.) and Missing (Mis.). For quantitative bacterial identification, S.p., H.i. and M.c. were confirmed by bacterial identification load higher than (\>) 10\^4 colony forming units per milliliter (cfu/mL), if bacterial species were present alone, or \>10\^5 cfu/mL if present as co-infection. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

197 participants

Primary outcome timeframe

From Day 0 up to Year 2

Results posted on

2019-10-04

Participant Flow

Participant milestones

Participant milestones
Measure
Total Group
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Overall Study
STARTED
197
Overall Study
COMPLETED
197
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Identification and Characterization of Bacteria in the Lungs of Children From 6 Months up to 6 Years Old, With Suspected Infection of the Lungs, in Spain.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Total Group
n=197 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Age, Continuous
40.7 Months
STANDARD_DEVIATION 18.2 • n=93 Participants
Sex: Female, Male
Female
95 Participants
n=93 Participants
Sex: Female, Male
Male
102 Participants
n=93 Participants

PRIMARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling results available.

Bacterial aetiology was assessed by culture growth from BAL fluid sample for Steptococcus pneumoniae (S.p.), Haemophilus influenzae (H.i.) and Moraxella catarrhalis (M.c.) , through either qualitative or quantitative bacterial identification (B.I.). The categories assessed were: positive (Pos.), negative (Neg.) and Missing (Mis.). For quantitative bacterial identification, S.p., H.i. and M.c. were confirmed by bacterial identification load higher than (\>) 10\^4 colony forming units per milliliter (cfu/mL), if bacterial species were present alone, or \>10\^5 cfu/mL if present as co-infection. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. qualitative B.I. for any of the 3 bacteria
141 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. qualitative B.I. for any of the 3 bacteria
49 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. qualitative B.I. for any of the 3 bacteria
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. qualitative B.I. for S. pneumoniae
58 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. qualitative B.I. for S. pneumoniae
132 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. qualitative B.I. for S. pneumoniae
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. qualitative B.I. for H. influenzae
97 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. qualitative B.I. for H. influenzae
93 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. qualitative B.I. for H. influenzae
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. qualitative B.I. for M. catarrhalis
94 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. qualitative B.I. for M. catarrhalis
96 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. qualitative B.I. for M. catarrhalis
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. qualitative B.I. for other bacteria
12 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. qualitative B.I. for other bacteria
0 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. qualitative B.I. for other bacteria
179 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. quantitative B.I. for any of the 3 bacteria
42 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. quantitative B.I. for any of the 3 bacteria
148 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. quantitative B.I. for any of the 3 bacteria
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. quantitative B.I. for S. pneumoniae
20 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. quantitative B.I. for S. pneumoniae
170 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. quantitative B.I. for S. pneumoniae
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. quantitative B.I. for H. influenzae
17 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. quantitative B.I. for H. influenzae
173 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. quantitative B.I. for H. influenzae
1 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Pos. quantitative B.I. for M. catarrhalis
12 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Neg. quantitative B.I. for M. catarrhalis
178 Participants
Number of Subjects With Bacterial Aetiology, Assessed by Culture Growth, From Bronchoalveolar Lavage (BAL) Fluid Samples
Mis. quantitative B.I. for M. catarrhalis
1 Participants

PRIMARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling results available.

S. pneumoniae (S.p.), H. influenzae (H.i.) and M. catarrhalis (M.c.) were confirmed by bacterial identification (B.I.) load higher than (\>) 10\^4 colony forming units per milliliter (cfu/mL), if bacterial species were present alone, or \>10\^5 cfu/mL if presented as co-infection. Analysis was also performed for other bacterial pathogens alone or as co-infection. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
S.p. alone (B.I. >10^4 cfu/mL)
15 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
H.i. alone (B.I. >10^4 cfu/mL)
14 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
M.c. alone (B.I. >10^4 cfu/mL)
6 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
Other bacterial pathogens - Any load
12 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
Other bacterial pathogens alone - Any load
10 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
S.p. and H.i. (B.I. >10^5 cfu/mL)
1 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
S.p. and M.c. (B.I. >10^5 cfu/mL)
4 Participants
Number of Subjects With Bacterial Aetiology Characteristics in BAL Fluid Samples
H.i. and M.c. (B.I. >10^5 cfu/mL)
2 Participants

PRIMARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with negative M.catarrhalis BAL Fluid samples results available.

S. pneumoniae, H. influenzae and M. catarrhalis were confirmed by bacterial identification load higher than (\>) 10\^4 colony forming units per milliliter (cfu/mL), if bacterial species were present alone. Bacterial load referred to Negative M. catarrhalis - Negative S. pneumoniae - Negative H. influenzae (N.M.c.-N.S.p.-N.H.i.), Negative M. catarrhalis - Negative S. pneumoniae - Positive H. influenzae (N.M.c.-N.S.p.-P.H.i.), Negative M. catarrhalis - Positive S. pneumoniae - Negative H. influenzae (N.M.c.-P.S.p.-N.H.i.), Negative M. catarrhalis - Positive S. pneumoniae - Positive H. influenzae (N.M.c.-P.S.p.-P..H.i.). Notes: bacterial identification for the N.M.c.-P.S.p.-P.H.i. category was confirmed as a co-infection with a bacterial load \>10\^5 cfu/mL. For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=178 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Negative M. Catarrhalis" BAL Fluid Samples Results Category)
N.M.c.-N.S.p.-N.H.i.
148 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Negative M. Catarrhalis" BAL Fluid Samples Results Category)
N.M.c.-N.S.p.-P.H.i.
14 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Negative M. Catarrhalis" BAL Fluid Samples Results Category)
N.M.c.-P.S.p.-N.H.i.
15 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Negative M. Catarrhalis" BAL Fluid Samples Results Category)
N.M.c.-P.S.p.-P.H.i.
1 Participants

PRIMARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with positive M.catarrhalis BAL Fluid samples results available.

S. pneumoniae, H. influenzae and M. catarrhalis were confirmed by bacterial identification load higher than (\>) 10\^4 colony forming units per milliliter (cfu/mL), if bacterial species were present alone and by bacterial load \>10\^5 cfu/mL if presented as co-infection. Categories referred to Positive M. catarrhalis -Negative S. pneumoniae - Negative H. influenzae (P.M.c.-N.S.p.-N.H.i.), Positive M. catarrhalis -Negative S. pneumoniae - Positive H. influenzae (P.M.c.-N.S.p.-P.H.i.), Positive M. catarrhalis - Positive S. pneumoniae - Negative H. influenzae (P.M.c.-P.S.p.-N.H.i.), Positive M. catarrhalis - Positive S. pneumoniae - Positive H. influenzae (P.M.c.-P.S.p.-P.H.i.). Notes: bacterial identification for the P.M.c.-N.S.p.-P.H.i., P.M.c.-P.S.p.-N.H.i. and P.M.c.-P.S.p.-P.H.i. categories was confirmed by a bacterial identification \>10\^5 cfu/mL. For one subject who underwent BAL procedure, no BAL fluid was available for study analyses

Outcome measures

Outcome measures
Measure
Total Group
n=12 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Positive M. Catarrhalis" BAL Fluid Samples Results Category)
P.M.c.-N.S.p.-N.H.i.
6 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Positive M. Catarrhalis" BAL Fluid Samples Results Category)
P.M.c.-N.S.p.-P.H.i.
2 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Positive M. Catarrhalis" BAL Fluid Samples Results Category)
P.M.c.-P.S.p.-N.H.i.
4 Participants
Number of Subjects With Bacterial Identification >10^4 Cfu/mL in BAL Fluid Samples (S. Pneumoniae and H. Influenzae Results for the "Positive M. Catarrhalis" BAL Fluid Samples Results Category)
P.M.c.-P.S.p.-P.H.i.
0 Participants

SECONDARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with nasopharyngeal swab sampling results available.

Bacterial colonization was assessed by culture growth from nasopharyngeal swab samples for S. pneumoniae, H. influenzae and M. catarrhalis, through either qualitative or quantitative bacterial identification (B.I.). The categories assessed were: positive (Pos.) and negative (Neg.).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. qualitative B.I. for any of the 3 bacteria
154 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. qualitative B.I. for any of the 3 bacteria
37 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. qualitative B.I. for S. pneumoniae
99 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. qualitative B.I. for S. pneumoniae
92 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. qualitative B.I. for H. influenzae
89 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. qualitative B.I. for H. influenzae
102 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. qualitative B.I. for M. catarrhalis
107 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. qualitative B.I. for M. catarrhalis
84 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. quantitative B.I. for any of the 3 bacteria
37 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. quantitative B.I. for any of the 3 bacteria
154 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. quantitative B.I. for S. pneumoniae
24 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. quantitative B.I. for S. pneumoniae
167 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. quantitative B.I. for H. influenzae
7 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. quantitative B.I. for H. influenzae
184 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Pos. quantitative B.I. for M. catarrhalis
10 Participants
Number of Subjects With Bacterial Colonization, Assessed by Culture Growth, From Nasopharyngeal Swab Samples
Neg. quantitative B.I. for M. catarrhalis
181 Participants

SECONDARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling results available for the outcome measure.

Bacterial load was detected by quantitative culture growth from BAL fluid samples for S. pneumoniae, H. influenzae and M. catarrhalis and expressed in colony-forming unit/ milliliter (cfu/mL). Quantitative bacterial identification was used to indicate the presence of bacterial pathogen load \>10\^4 cfu/mL if present alone or \> 10\^5 cfu/mL if present as co-infection. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=97 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Bacterial Load Detected (log10 Transformation) by Quantitative Culture Growth, From BAL Fluid Samples
S. pneumoniae Bacterial load
4.28 cfu/mL
Standard Deviation 1.04
Bacterial Load Detected (log10 Transformation) by Quantitative Culture Growth, From BAL Fluid Samples
H. influenzae Bacterial load
3.81 cfu/mL
Standard Deviation 1.11
Bacterial Load Detected (log10 Transformation) by Quantitative Culture Growth, From BAL Fluid Samples
M. catarrhalis Bacterial load
3.54 cfu/mL
Standard Deviation 1.01

SECONDARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling results available for the outcome measure.

Bacterial load was detected by quantitative molecular techniques (PCR) from BAL fluid samples for S. pneumoniae, H. influenzae and M. catarrhalis and expressed in colony-forming unit/milliliter (cfu/mL). Quantitative bacterial identification was used to indicate the presence of bacterial pathogen load \>10\^4 cfu/mL if present alone or \> 10\^5 cfu/mL if present as co-infection. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=138 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Bacterial Load Detected (log10 Transformation) by Quantitative Molecular Techniques (Polymerase Chain Reaction) From BAL Fluid Samples
S. pneumoniae Bacterial load
4.06 cfu/mL
Standard Deviation 1.27
Bacterial Load Detected (log10 Transformation) by Quantitative Molecular Techniques (Polymerase Chain Reaction) From BAL Fluid Samples
H. influenzae Bacterial load
4.31 cfu/mL
Standard Deviation 1.32
Bacterial Load Detected (log10 Transformation) by Quantitative Molecular Techniques (Polymerase Chain Reaction) From BAL Fluid Samples
M. catarrhalis Bacterial load
3.92 cfu/mL
Standard Deviation 1.29

SECONDARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling results available.

Bacterial pathogens were detected by qualitative culture from BAL fluid samples and included: Staphylococcus aureus, Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Pseudomonas putida. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture, From BAL Fluid Samples
Staphylococcus aureus (Growth)
1 Participants
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture, From BAL Fluid Samples
Pseudomonas aeruginosa (Growth)
5 Participants
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture, From BAL Fluid Samples
Stenotrophomonas maltophilia (Growth)
4 Participants
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture, From BAL Fluid Samples
Pseudomonas putida (Growth)
2 Participants

SECONDARY outcome

Timeframe: From Day 0 up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with nasopharyngeal swab sampling results available for the outcome measure.

Bacterial load was detected by quantitative culture from nasopharyngeal swab samples for S. pneumoniae, H. influenzae and M. catarrhalis and expressed in colony-forming unit/ milliliter (cfu/mL). Quantitative bacterial identification was used to indicate the presence of bacterial pathogen load \>10\^4 cfu/mL if present alone or \> 10\^5 cfu/mL if present as co-infection.

Outcome measures

Outcome measures
Measure
Total Group
n=107 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Bacterial Load Detected (log10 Transformation) by Quantitative Culture From Nasopharyngeal Swab Samples
H. influenzae Bacterial load
3.61 cfu/mL
Standard Deviation 0.88
Bacterial Load Detected (log10 Transformation) by Quantitative Culture From Nasopharyngeal Swab Samples
S. pneumoniae Bacterial load
3.99 cfu/mL
Standard Deviation 1.15
Bacterial Load Detected (log10 Transformation) by Quantitative Culture From Nasopharyngeal Swab Samples
M. catarrhalis Bacterial load
3.36 cfu/mL
Standard Deviation 1.05

SECONDARY outcome

Timeframe: From Day 0 and up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with nasopharyngeal swab sampling results available for the outcome measure.

Bacterial load was detected by quantitative molecular techniques from nasopharyngeal swab samples for S. pneumoniae, H. influenzae and M. catarrhalis and expressed in colony-forming unit/milliliter (cfu/mL). Quantitative bacterial identification was used to indicate the presence of bacterial pathogen load \>10\^4 cfu/mL if present alone or \> 10\^5 cfu/mL if present as co-infection.

Outcome measures

Outcome measures
Measure
Total Group
n=133 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Bacterial Load Detected (log10 Transformation) by Molecular Techniques (PCR) From Nasopharyngeal Swab Samples
S. pneumoniae Bacterial load
4.39 cfu/mL
Standard Deviation 1.13
Bacterial Load Detected (log10 Transformation) by Molecular Techniques (PCR) From Nasopharyngeal Swab Samples
H. influenzae Bacterial load
4.16 cfu/mL
Standard Deviation 1.17
Bacterial Load Detected (log10 Transformation) by Molecular Techniques (PCR) From Nasopharyngeal Swab Samples
M. catarrhalis Bacterial load
4.05 cfu/mL
Standard Deviation 1.24

SECONDARY outcome

Timeframe: From Day 0 and up to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with nasopharyngeal swab sampling results available.

Bacterial pathogens were detected by qualitative culture from nasopharyngeal swab samples and included: Staphylococcus aureus and Stenotrophomonas maltophilia

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture From Nasopharyngeal Swab Samples
Stenotrophomonas maltophilia (Growth)
1 Participants
Number of Subjects With Other Bacterial Pathogens Detected by Qualitative Culture From Nasopharyngeal Swab Samples
Staphylococcus aureus (Growth)
6 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable unique isolates of subjects for S. pneumoniae, who complied with the protocol and with BAL fluid sampling results available.

Two isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. S.p. serogoups and serotypes isolates included: 3, 6B, 7C, 10A, 11A, 11D, 12F, 14, 15B, 16F, 18C, 18F, 19A, 19F, 21, 22F, 23A, 23B, 31, 35F, 42, 48, NT (not typeable), Synflorix serotypes (1, 4, 5, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F) and Pneumococcal conjugate vaccine (PCV) 13 serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F). Note: For one subject who underwent BAL procedure, no BALF was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=65 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
3
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
6B
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
7C
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
10A
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
11A
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
11D
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
12F
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
14
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
15B
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
16F
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
18C
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
18F
10 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
19A
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
19F
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
21
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
22F
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
23A
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
23B
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
31
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
35F
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
42
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
48
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
NT
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
Synflorix serotypes
15 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes in BAL Fluid Samples
PCV13 serotypes
17 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable unique isolates of subjects for H. influenzae, who complied with the protocol and with BAL fluid sampling results available.

The H. influenzae typing results included "f" and "NT" (not typeable). Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BALF was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=98 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Qualitative Positive Unique Isolates of Subjects With H. Influenzae Typing Results From BAL Fluid Samples
f
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With H. Influenzae Typing Results From BAL Fluid Samples
NT
95 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable unique isolates of subjects for S. pneumoniae, who complied with the protocol and with nasopharyngeal swab sampling results available.

S. pneumoniae serogoups and serotypes included: 3, 6A, 6B, 7C, 9N, 10A, 11A, 11D, 12B, 12F, 14, 16F, 18C, 18F, 19A, 19C, 19F, 21, 23A, 23B, 31, 35A, 35F, 42, 48, NT (not typeable), Synflorix serotypes (1, 4, 5, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F) and Pneumococcal conjugate vaccine (PCV) 13 serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 23F). Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=105 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
3
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
6A
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
6B
6 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
7C
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
9N
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
10A
5 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
11A
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
11D
5 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
12B
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
12F
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
14
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
16F
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
18C
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
18F
14 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
19A
6 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
19C
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
19F
9 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
21
7 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
23A
5 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
23B
2 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
31
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
35A
1 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
35F
3 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
42
6 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
48
4 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
NT
11 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
Synflorix serotypes
24 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With S. Pneumoniae Serogroups and Serotypes From Nasopharyngeal Swab Samples
PCV13 serotypes
27 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable unique isolates of subjects for H. influenzae, who complied with the protocol and with nasopharyngeal swab sampling results available.

The H. influenzae typing results include "f " and "NT" (not typeable). Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=92 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Qualitative Positive Unique Isolates of Subjects With H. Influenzae Typing Results From Nasopharyngeal Swab Samples
NT
88 unique isolates of subjects
Number of Qualitative Positive Unique Isolates of Subjects With H. Influenzae Typing Results From Nasopharyngeal Swab Samples
f
4 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the S. pneumoniae unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antibiotic response of S.pneumoniae was susceptible (Sus), or intermediate (Int), or resistant (Res)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]) towards the tested antibiotics which included: Penicilin, Amoxicillin/Clavulanate, Erythromycin, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcome description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=105 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Penicilin (NPS)
55 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (BALF)
41 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Erythromycin (BALF)
21 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Penicilin (BALF)
36 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Penicilin (BALF)
13 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Penicilin (BALF)
16 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Penicilin (NPS)
27 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Penicilin (NPS)
23 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/ Clavulanate (BALF)
50 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/ Clavulanate (BALF)
7 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/ Clavulanate (BALF)
8 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/ Clavulanate (NPS)
86 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/ Clavulanate (NPS)
10 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/ Clavulanate (NPS)
9 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Erythromycin (BALF)
43 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Erythromycin (BALF)
1 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Erythromycin (NPS)
55 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Erythromycin (NPS)
1 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Erythromycin (NPS)
49 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (BALF)
3 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (BALF)
21 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (NPS)
57 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (NPS)
4 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (NPS)
44 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (BALF)
51 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (BALF)
14 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (BALF)
0 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (NPS)
71 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (NPS)
3 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (NPS)
31 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (BALF)
65 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (BALF)
0 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (BALF)
0 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (NPS)
103 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (NPS)
0 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (NPS)
2 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
24 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
13 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
28 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
52 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
13 unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (NPS)
40 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antibiotic response of H.ifluenzae was susceptible (Sus), or intermediate (Int), or resistant (Res)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]) towards the tested antibiotics which included: Amoxicillin/Clavulanate, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcomes description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=98 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (NPS)
92 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (NPS)
3 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (BALF)
98 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (BALF)
98 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (NPS)
92 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
51 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
16 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
31 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
49 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
18 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (NPS)
25 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/ Clavulanate (BALF)
91 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/ Clavulanate (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/ Clavulanate (BALF)
7 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/ Clavulanate (NPS)
91 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/ Clavulanate (NPS)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/ Clavulanate (NPS)
1 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (BALF)
95 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (BALF)
0 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (BALF)
3 unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (NPS)
89 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antibiotic response of H. influenzae was tested against antibiotics which included: Penicilin and Erythromycin (following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]). Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=98 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Descriptive Statistics of the Antimicrobial Susceptibility Response of H. Influenzae for Unique Isolates Among Qualitative Positive Subjects, for Penicilin and Erythromycin
Penicilin (BALF)
0.5 unique isolates of subjects
Interval 0.13 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H. Influenzae for Unique Isolates Among Qualitative Positive Subjects, for Penicilin and Erythromycin
Penicilin (NPS)
0.5 unique isolates of subjects
Interval 0.13 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H. Influenzae for Unique Isolates Among Qualitative Positive Subjects, for Penicilin and Erythromycin
Erythromycin (BALF)
4 unique isolates of subjects
Interval 1.0 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H. Influenzae for Unique Isolates Among Qualitative Positive Subjects, for Penicilin and Erythromycin
Erythromycin (NPS)
4.00 unique isolates of subjects
Interval 0.25 to 16.0

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the M. catarrhalis unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antibiotic response of M. catarrhalis was susceptible (Sus), or intermediate (Int), or resistant (Res)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]) towards the tested antibiotics which included: Amoxicillin/Clavulanate, Erythromycin, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcome description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=110 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/Clavulanate (BALF)
95 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/Clavulanate (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/Clavulanate (BALF)
3 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Amoxicillin/Clavulanate (NPS)
105 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Amoxicillin/Clavulanate (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Amoxicillin/Clavulanate (NPS)
5 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Erythromycin (BALF)
90 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Erythromycin (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Erythromycin (BALF)
8 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Erythromycin (NPS)
106 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Erythromycin (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Erythromycin (NPS)
4 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (BALF)
84 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (BALF)
14 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Azithromycin (NPS)
106 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Azithromycin (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Azithromycin (NPS)
4 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (BALF)
98 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Tetracycline (NPS)
110 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Tetracycline (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Tetracycline (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (BALF)
98 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (BALF)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Levofloxacin (NPS)
110 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Levofloxacin (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Levofloxacin (NPS)
0 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
56 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
35 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
7 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
103 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
7 unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response, Among Qualitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole(NPS)
0 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the M. catarrhalis unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antibiotic response of M. catarrhalis was tested against antibiotics which included: Penicilin. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=110 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Descriptive Statistics of the Antimicrobial Susceptibility Response of M. Catarrhalis for Unique Isolates Among Qualitative Positive Subjects, for Penicilin
Penicilin (BALF)
8.00 unique isolates of subjects
Interval 0.02 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of M. Catarrhalis for Unique Isolates Among Qualitative Positive Subjects, for Penicilin
Penicilin (NPS)
16.00 unique isolates of subjects
Interval 0.02 to 16.0

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae and M. catarrhalis unique isolates from qualitative positive subjects who were included in the ATP cohort.

Antimicrobial response of H. influenzae and M. catarrhalis was tested for Beta-lactamase (following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]). Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=110 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of H. Influenzae or M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response Among Qualitative Positive Siubjects for Beta-lactamase
H. influenzae (BALF)
7 unique isolates of subjects
Number of H. Influenzae or M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response Among Qualitative Positive Siubjects for Beta-lactamase
H. influenzae (NPS)
9 unique isolates of subjects
Number of H. Influenzae or M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response Among Qualitative Positive Siubjects for Beta-lactamase
M. catarrhalis (BALF)
93 unique isolates of subjects
Number of H. Influenzae or M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response Among Qualitative Positive Siubjects for Beta-lactamase
M. catarrhalis (NPS)
108 unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the S. pneumoniae unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antibiotic response of S.pneumoniae was susceptible (Sus), or intermediate (Int), or resistant (Res)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]) towards the tested antibiotics which included: Penicilin, Amoxicillin/Clavulanate, Erythromycin, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcome description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=26 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Penicilin (BALF)
4 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Penicilin (BALF)
15 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Penicilin (BALF)
2 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Penicilin (NPS)
11 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Penicilin (NPS)
10 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Penicilin (NPS)
5 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (BALF)
17 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (BALF)
3 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (BALF)
1 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (NPS)
22 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (NPS)
3 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (NPS)
1 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Erythromycin (BALF)
17 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Erythromycin (BALF)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Erythromycin (BALF)
4 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Erythromycin (NPS)
11 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Erythromycin (NPS)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Erythromycin (NPS)
15 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (BALF)
15 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (BALF)
2 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (BALF)
4 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (NPS)
11 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (NPS)
2 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (NPS)
13 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (BALF)
18 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (BALF)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (BALF)
3 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (NPS)
16 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (NPS)
1 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (NPS)
9 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (BALF)
21 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (NPS)
25 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (NPS)
0 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (NPS)
1 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
3 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
5 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
13 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
12 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
2 Unique isolates of subjects
Number of S. Pneumoniae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (NPS)
12 Unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antibiotic response of H.ifluenzae was susceptible (Sus), or intermediate (Int), or resistant (Res)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\])towards the tested antibiotics which included: Amoxicillin/Clavulanate, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcomes description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=18 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
5 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (BALF)
15 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (BALF)
3 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (NPS)
8 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (BALF)
17 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (BALF)
1 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (NPS)
8 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (BALF)
18 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (NPS)
8 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (BALF)
18 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (NPS)
8 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (NPS)
0 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
5 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
7 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
6 Unique isolates of subjects
Number of H. Influenzae Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (NPS)
3 Unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antibiotic response of H.ifluenzae was tested against antibiotics which included: Penicilin and Erythromycin (following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]). Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=18 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Descriptive Statistics of the Antimicrobial Susceptibility Response of H.Influenzae for Unique Isolates Among Quantitative Positive Subjects, for Penicilin and Erythromycin
Penicilin (BALF)
0.50 Unique isolates of subjects
Interval 0.25 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H.Influenzae for Unique Isolates Among Quantitative Positive Subjects, for Penicilin and Erythromycin
Penicilin (NPS)
0.5 Unique isolates of subjects
Interval 0.13 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H.Influenzae for Unique Isolates Among Quantitative Positive Subjects, for Penicilin and Erythromycin
Erythromycin (BALF)
4.00 Unique isolates of subjects
Interval 2.0 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of H.Influenzae for Unique Isolates Among Quantitative Positive Subjects, for Penicilin and Erythromycin
Erythromycin (NPS)
4.00 Unique isolates of subjects
Interval 2.0 to 8.0

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the M. catarrhalis unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antibiotic response of M. catarrhalis was susceptible (Sus.), or intermediate (Int.), or resistant (Res.)(following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]) towards the tested antibiotics which included: Amoxicillin/Clavulanate, Erythromycin, Azithromycin, Tetracycline, Levofloxacin, Trimethoprim/Sulfamethoxazole. Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. For the "Unique isolate definition", please refer to the previous outcome description. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=13 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (BALF)
12 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (BALF)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Amoxicillin/Clavulanate (NPS)
9 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Amoxicillin/Clavulanate (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Amoxicillin/Clavulanate (NPS)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Erythromycin (BALF)
12 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Erythromycin (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Erythromycin (BALF)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Erythromycin (NPS)
9 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Erythromycin (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Erythromycin (NPS)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (BALF)
11 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (BALF)
2 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Azithromycin (NPS)
9 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Azithromycin (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Azithromycin (NPS)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (BALF)
13 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Tetracycline (NPS)
10 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Tetracycline (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Tetracycline (NPS
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (BALF)
13 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (BALF)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Levofloxacin (NPS)
10 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Levofloxacin (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Levofloxacin (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (BALF)
7 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (BALF)
5 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (BALF)
1 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Sus. Trimethoprim/Sulfamethoxazole (NPS)
10 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Int. Trimethoprim/Sulfamethoxazole (NPS)
0 Unique isolates of subjects
Number of M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Response From Quantitative Positive Subjects
Res. Trimethoprim/Sulfamethoxazole (NPS)
0 Unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the M. catarrhalis unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antibiotic response of M. catarrhalis was tested against antibiotics which included: Penicilin (following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]). Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=13 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Descriptive Statistics of the Antimicrobial Susceptibility Response of M. Catarrhalis for Unique Isolates Among Quantitative Positive Subjects, for Penicilin
Penicilin (BALF)
16.00 Unique isolates of subjects
Interval 0.5 to 16.0
Descriptive Statistics of the Antimicrobial Susceptibility Response of M. Catarrhalis for Unique Isolates Among Quantitative Positive Subjects, for Penicilin
Penicilin (NPS)
16.00 Unique isolates of subjects
Interval 1.0 to 16.0

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the H. influenzae or M. catarrhalis unique isolates from quantitative positive subjects who were included in the ATP cohort.

Antimicrobial response of H. influenzae and M. catarrhalis was tested for Beta-lactamase (following Clinical and Laboratory Standards Institute \[CLSI\] guidelines \[CLSI, 2015\]). Samples assessed were BAL fluid (BALF) samples and Nasopharyngeal swab (NPS) samples. Unique isolate definition: 2 isolates of S.p., H.i. and M.c. were selected per specimen type per subjects. For analyses, the 2 isolates were compared to determine if they were clones vs. different ("unique") pathogen. Isolates were considered "unique" based on the comparison of serotype and antibiotic susceptibility (AS) profile for S.p. and H.i. and on the AS profile alone for M.c.. If the 2 isolates had different serotypes, or the same serotype but a different AS profile, then the 2 isolates were considered "unique", and both isolates were retained in the analyses. Note: For one subject who underwent BAL procedure, no BAL fluid was available for study analyses.

Outcome measures

Outcome measures
Measure
Total Group
n=18 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of H. Influenzae and M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response From Quantitative Positive Subjects, for Beta-lactamase
H. influenzae (BALF)
3 Unique isolates of subjects
Number of H. Influenzae and M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response From Quantitative Positive Subjects, for Beta-lactamase
H. influenzae (NPS)
1 Unique isolates of subjects
Number of H. Influenzae and M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response From Quantitative Positive Subjects, for Beta-lactamase
M. catarrhalis (BALF)
13 Unique isolates of subjects
Number of H. Influenzae and M. Catarrhalis Unique Isolates With Antimicrobial Susceptibility Positive Response From Quantitative Positive Subjects, for Beta-lactamase
M. catarrhalis (NPS)
10 Unique isolates of subjects

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Demographic characteristics included age, which was expressed in months.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Mean Age of Subjects
39.84 Months
Standard Deviation 17.76

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Demographic characteristics included gender: female and male.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Subject Gender
Gender (Female)
92 Participants
Subject Gender
Gender (Male)
99 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Demographic characteristics included weight which was expressed in kilograms (kg).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Mean Weight of Subjects
14.67 Kilograms
Standard Deviation 3.83

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Demographic characteristics included height which was expressed in centimeters (cm).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Mean Height of Subjects
96.04 Centimeters
Standard Deviation 13.10

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Demographic characteristics included body mass index (BMI) whose z-scores were measures of relative weight adjusted for child age and sex.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Marked thinness [< z-score (-3)]
5 Participants
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Thinness [≥ z-score (-3) and < z-score (-2)]
9 Participants
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Normal [≥ z-score (-2) and ≤ z-score (1)]
122 Participants
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Overweight [> z-score (1) and ≤ z-score (2)]
26 Participants
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Obesity [> z-score (2) and ≤ z-score (3)]
14 Participants
Number of Subjects With Body Mass Index (BMI) Characteristics
BMI - Extreme obesity [> z-score (3)]
4 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

General medical history characteristics included: any pre-existing conditions, congenital chromosomal abnormality, prematurity (less than 37 weeks) neonatal problems, chronic renal failure, immune system disorder (including auto-immune), atopic dermatitis, clinician-confirmed eczema, asthma and other.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects Presenting General Medical History Characteristics
Any other pre-existing conditions (Yes)
176 Participants
Number of Subjects Presenting General Medical History Characteristics
Any other pre-existing conditions (No)
15 Participants
Number of Subjects Presenting General Medical History Characteristics
Congenital chromosomal abnormality (Yes)
2 Participants
Number of Subjects Presenting General Medical History Characteristics
Prematurity (<37 weeks) (Yes)
33 Participants
Number of Subjects Presenting General Medical History Characteristics
Neonatal problems (Yes)
18 Participants
Number of Subjects Presenting General Medical History Characteristics
Chronic renal failure (Yes)
1 Participants
Number of Subjects Presenting General Medical History Characteristics
Immune system disorder (Yes)
1 Participants
Number of Subjects Presenting General Medical History Characteristics
History of inhaled steroids (Yes)
134 Participants
Number of Subjects Presenting General Medical History Characteristics
Atopic dermatitis (Yes)
49 Participants
Number of Subjects Presenting General Medical History Characteristics
Clinician-confirmed eczema (Yes)
4 Participants
Number of Subjects Presenting General Medical History Characteristics
Asthma (Yes)
72 Participants
Number of Subjects Presenting General Medical History Characteristics
Other (Yes)
117 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

General medical history included mean gestational age.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Mean Gestational Age of Subjects
33.00 Weeks
Standard Deviation 2.95

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Pneumococcal vaccination history characteristics included vaccination, pneumococcal vaccines received (Prevnar only, Prevnar 13 only, Synflorix only, Mix of vaccines), Prevnar only doses (1-4), Prevnar 13 only doses (1-4), Synflorix only doses (4), medical history source validated or not.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Pneumococcal Vaccination History Characteristics
Child has received any PCV (Yes)
168 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Child has received only Prevenar
15 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Child has received only Prevenar 13
123 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Child has received only Synflorix
1 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Child has received Mix of vaccines
29 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar (1)
2 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar (2)
4 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar (3)
3 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar (4)
6 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar 13 (1)
4 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar 13 (2)
12 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar 13 (3)
46 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Prevenar 13 (4)
61 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Doses of Synflorix (4)
1 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Validated source
165 Participants
Number of Subjects With Pneumococcal Vaccination History Characteristics
Not validated source
3 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Flu vaccination history included vaccination, Flu vaccine doses (1-5), medical history source validated or not.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Flu Vaccination History Characteristics
Child has ever received any flu vaccine (Yes)
58 Participants
Number of Subjects With Flu Vaccination History Characteristics
Number of doses of any flu vaccine (1)
18 Participants
Number of Subjects With Flu Vaccination History Characteristics
Number of doses of any flu vaccine (2)
26 Participants
Number of Subjects With Flu Vaccination History Characteristics
Number of doses of any flu vaccine (3)
6 Participants
Number of Subjects With Flu Vaccination History Characteristics
Number of doses of any flu vaccine (4)
5 Participants
Number of Subjects With Flu Vaccination History Characteristics
Number of doses of any flu vaccine (5)
3 Participants
Number of Subjects With Flu Vaccination History Characteristics
Validated source
52 Participants
Number of Subjects With Flu Vaccination History Characteristics
Not validated source
6 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Haemophilus influenzae type b vaccination history included vaccination, Haemophilus influenzae type b vaccine doses (1-4), medical history source validated or not.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Child has received H.I. type b vaccine (Yes)
188 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Number of doses of H.I. type b vaccine (1)
2 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Number of doses of H.I. type b vaccine (2)
4 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Number of doses of H.I. type b vaccine (3)
38 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Number of doses of H.I. type b vaccine (4)
144 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Validated source
184 Participants
Number of Subjects With Haemophilus Influenzae Type b Vaccination History Characteristics
Not validated source
4 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Medication history referred to antibiotics administered in the past 6 months, drug names, antibiotic information source, other medications administered during the past 6 months, information source.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Antibiotics and Other Medications Administered
Antibiotic administered in the past 6 months (Yes)
153 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Penicillin
83 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Amoxicillin/ Clavulanate
76 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Cephalosporins
24 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Erythromycin
1 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Azithromycin
57 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Clarithromycin
8 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Ciprofloxacin
2 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Clindamycin
1 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Linezolid
1 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Meropenem
1 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Antibiotics source information. - parent recall
49 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Antibiotics source information. - medical file
120 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Other medications in the past 6 months (Yes)
177 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Source of information. - parent recall
49 Participants
Number of Subjects With Antibiotics and Other Medications Administered
Source of information. - medical file
149 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Clinical characteristics included affections/pre-conditions, affection episodes, number of children living in a household, children in day-care centres, exposure to cigarette smoke.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Clinical Characteristics
Shortness of breath secondary to cough (Yes)
33 Participants
Number of Subjects With Clinical Characteristics
Cough (Yes)
151 Participants
Number of Subjects With Clinical Characteristics
Wheezing (Yes)
86 Participants
Number of Subjects With Clinical Characteristics
Pathologic auscultation (Yes)
74 Participants
Number of Subjects With Clinical Characteristics
Infiltrates atelectasis diagnosed by X-ray (Yes)
131 Participants
Number of Subjects With Clinical Characteristics
Wet cough lasting 4 weeks without additional (Yes)
63 Participants
Number of Subjects With Clinical Characteristics
Persistent wheezing lasting 3 months (Yes)
8 Participants
Number of Subjects With Clinical Characteristics
Recurrent wheezing 3 or more episodes/year (Yes)
25 Participants
Number of Subjects With Clinical Characteristics
Persistent pathologic auscultation (Yes)
16 Participants
Number of Subjects With Clinical Characteristics
Persistent 1 month infiltrates atelectasis (Yes)
65 Participants
Number of Subjects With Clinical Characteristics
3 episodes/year infiltrates atelectasis (Yes)
14 Participants
Number of Subjects With Clinical Characteristics
Any respiratory/otitis media pre-conditions(Yes)
186 Participants
Number of Subjects With Clinical Characteristics
Any respiratory/otitis media pre- conditions (No)
5 Participants
Number of Subjects With Clinical Characteristics
Pneumonia (Yes)
114 Participants
Number of Subjects With Clinical Characteristics
Bronchiolitis (Yes)
85 Participants
Number of Subjects With Clinical Characteristics
Bronchioectasis (Yes)
15 Participants
Number of Subjects With Clinical Characteristics
Bronchitis (Yes)
143 Participants
Number of Subjects With Clinical Characteristics
Rhinitis (Yes)
15 Participants
Number of Subjects With Clinical Characteristics
Acute otitis media (Yes)
53 Participants
Number of Subjects With Clinical Characteristics
Otitis media with effusion or glue ear (Yes)
6 Participants
Number of Subjects With Clinical Characteristics
Otitis media with perforation and discharge (Yes)
8 Participants
Number of Subjects With Clinical Characteristics
The child had ever been breastfed (Yes)
150 Participants
Number of Subjects With Clinical Characteristics
The child had ever been breastfed (No)
41 Participants
Number of Subjects With Clinical Characteristics
The child was currently breastfed (Yes)
6 Participants
Number of Subjects With Clinical Characteristics
The child was currently breastfed (No)
144 Participants
Number of Subjects With Clinical Characteristics
Other children <6 years in the same household (0)
101 Participants
Number of Subjects With Clinical Characteristics
children <6 years in the same household (1-2)
87 Participants
Number of Subjects With Clinical Characteristics
children <6 years in the same household (≥3)
3 Participants
Number of Subjects With Clinical Characteristics
children ≥ 6 years in the same household (0)
134 Participants
Number of Subjects With Clinical Characteristics
children ≥ 6 years in the same household (1-2)
55 Participants
Number of Subjects With Clinical Characteristics
children ≥ 6 years in the same household (≥3)
2 Participants
Number of Subjects With Clinical Characteristics
Child at daycare &/school at least 2 day/week(Yes)
155 Participants
Number of Subjects With Clinical Characteristics
Child at day care &/school at least 2 day/week(No)
36 Participants
Number of Subjects With Clinical Characteristics
Child lived within 1 km of major polluters (Yes)
63 Participants
Number of Subjects With Clinical Characteristics
Child lived within 1 km of major polluters (No)
128 Participants
Number of Subjects With Clinical Characteristics
Major roadway (Yes)
20 Participants
Number of Subjects With Clinical Characteristics
Highway (Yes)
27 Participants
Number of Subjects With Clinical Characteristics
Area where trucks or vehicles were idle (Yes)
11 Participants
Number of Subjects With Clinical Characteristics
Major industry area with smokestacks (Yes)
13 Participants
Number of Subjects With Clinical Characteristics
Other (Yes)
6 Participants
Number of Subjects With Clinical Characteristics
Child with>3 day/week to cigarette smoke(Yes)
38 Participants
Number of Subjects With Clinical Characteristics
Child with>3 day/ week to cigarette smoked (No)
153 Participants
Number of Subjects With Clinical Characteristics
Previous episodes within the last year (0)
2 Participants
Number of Subjects With Clinical Characteristics
Previous episodes within the last year (1)
11 Participants
Number of Subjects With Clinical Characteristics
Previous episodes within the last year (2-3)
52 Participants
Number of Subjects With Clinical Characteristics
Previous episodes within the last year (4-5)
45 Participants
Number of Subjects With Clinical Characteristics
Previous episodes within the last year (6 or more)
73 Participants
Number of Subjects With Clinical Characteristics
Cough productive or wet (Yes)
153 Participants
Number of Subjects With Clinical Characteristics
Cough paroxysmal (Yes)
29 Participants
Number of Subjects With Clinical Characteristics
Cough laryngeal (Yes)
38 Participants
Number of Subjects With Clinical Characteristics
Shortness of breath independent of coughing (Yes)
35 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Laboratory results included blood sample results available, white blood Cell (WBC) count analysed, WBC reference range, Clinically relevant to the suspected chronic lower respiratory tract infection (LRTI) in the child if out of the range of WBC \[LRTI-WBC\], CRP (C-reactive protein) reference range, CRP groups, Clinically relevant to the suspected chronic LRTI in the child if out of the range of CRP \[LRTI-CRP\], ESR (Erythrocyte sedimentation rate) analysed, ESR reference range and Clinically relevant to the suspected chronic LRTI in the child if out of the range of ESR \[LRTI-ESR\].

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Laboratory Results
LRTI-ESR (No)
3 Participants
Number of Subjects With Laboratory Results
CRP reference range (missing range)
52 Participants
Number of Subjects With Laboratory Results
Blood sample results available (Yes)
109 Participants
Number of Subjects With Laboratory Results
WBC count analysed (Yes)
109 Participants
Number of Subjects With Laboratory Results
WBC reference range (low-high ranges)
78 Participants
Number of Subjects With Laboratory Results
WBC reference range (>high range)
31 Participants
Number of Subjects With Laboratory Results
LRTI-WBC (Yes)
18 Participants
Number of Subjects With Laboratory Results
LRTI-WBC (No)
17 Participants
Number of Subjects With Laboratory Results
CRP analysed (Yes)
57 Participants
Number of Subjects With Laboratory Results
CRP analysed (No)
52 Participants
Number of Subjects With Laboratory Results
CRP reference range (low-high ranges)
26 Participants
Number of Subjects With Laboratory Results
CRP reference range (>high range)
31 Participants
Number of Subjects With Laboratory Results
CRP groups <40 mg/L
46 Participants
Number of Subjects With Laboratory Results
CRP groups ≥40-80 mg/L
6 Participants
Number of Subjects With Laboratory Results
CRP groups ≥80-120 mg/L
3 Participants
Number of Subjects With Laboratory Results
CRP groups ≥120 mg/L
2 Participants
Number of Subjects With Laboratory Results
LRTI-CRP (Yes)
17 Participants
Number of Subjects With Laboratory Results
LRTI-CRP (No)
17 Participants
Number of Subjects With Laboratory Results
ESR analysed (Yes)
12 Participants
Number of Subjects With Laboratory Results
ESR analysed (No)
97 Participants
Number of Subjects With Laboratory Results
ESR reference range (low-high ranges)
8 Participants
Number of Subjects With Laboratory Results
ESR reference range (>high range)
4 Participants
Number of Subjects With Laboratory Results
ESR reference range (missing range)
97 Participants
Number of Subjects With Laboratory Results
LRTI-ESR (Yes)
2 Participants

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Laboratory results for WBC were expressed in 10\^9 cells per liter (10\^9 cells/L).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
White Blood Cells (WBC) Laboratory Results
540.17 10^9 cells/L
Standard Deviation 2651.17

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Laboratory results for CRP were expressed in milligrams per litre (mg/L).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
C-reactive Protein (CRP) Laboratory Results
25.45 mg/L
Standard Deviation 37.91

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Laboratory results for ESR were expressed in millimeters per hour (mm/hr).

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Erythrocyte Sedimentation Rate (ESR) Laboratory Results
25.33 mm/hr
Standard Deviation 30.67

SECONDARY outcome

Timeframe: From Day 0 to Year 2

Population: The analysis was performed on the According to protocol (ATP) cohort, which included all evaluable subjects, who complied with the protocol and with BAL fluid sampling and/ or nasopharyngeal swab sampling results available.

Radiological results included x-ray, result normality, relationship of abnormality to BAL sampling.

Outcome measures

Outcome measures
Measure
Total Group
n=191 Participants
All enrolled subjects, male or female aged between 6 months included and less than 6 years at the time of enrollment, who visited the hospital with suspected chronic lower respiratory tract infections (LRTIs) and who had an indication for bronchoalveolar lavage (BAL).
Number of Subjects With Radiological Results
Abnormality relevant to collecting the BALF (No)
21 Participants
Number of Subjects With Radiological Results
A Chest X-ray was done (Yes)
166 Participants
Number of Subjects With Radiological Results
A Chest X-ray was done (No)
25 Participants
Number of Subjects With Radiological Results
Result (Normal)
23 Participants
Number of Subjects With Radiological Results
Result (Abnormal)
143 Participants
Number of Subjects With Radiological Results
Abnormality relevant to collecting the BALF(Yes)
122 Participants

Adverse Events

Total Group

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

GSK Response Center

GlaxoSmithKline

Phone: 866-435-7343

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place