L-NMMA Plus Taxane Chemotherapy in Refractory Locally Advanced or Metastatic Triple Negative Breast Cancer Patients

NCT ID: NCT02834403

Last Updated: 2023-12-15

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

37 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-11-30

Study Completion Date

2021-01-31

Brief Summary

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This is a Phase Ib/II study assessing the maximum tolerated dose (MTD), dose-limiting toxicities (DLTs), recommended Phase 2 dose (RP2D), and efficacy of L-NMMA when combined with docetaxel in refractory locally advanced or metastatic triple negative breast cancer patients. The Phase Ib portion of the study is designed to investigate the combination at two dose levels of docetaxel (75 and 100 mg/m2) and 7 dose levels of L-NMMA (5, 7.5, 10, 12.5, 15, 17.5, and 20 mg/kg). The starting dose of L-NMMA will be 7.5 mg/kg. In the Phase II portion of the study, the starting dose will be the RP2D determined in the Phase Ib portion of the study.

Detailed Description

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This is a Phase Ib/II study assessing the maximum tolerated dose (MTD), dose-limiting toxicities (DLTs), recommended Phase 2 dose (RP2D), and efficacy of L-NMMA when combined with docetaxel in refractory locally advanced or metastatic triple negative breast cancer patients. The Phase Ib portion of the study is designed to investigate the combination at two dose levels of docetaxel (75 and 100 mg/m2) and 7 dose levels of L-NMMA (5, 7.5, 10, 12.5, 15, 17.5, and 20 mg/kg). The starting dose of L-NMMA will be 7.5 mg/kg. L-NMMA dose will escalate/de-escalate based on DLT occurrence. For the 5, 7.5, 10, 12.5, and 15 mg/kg L-NMMA doses, docetaxel will be administered at 75 mg/m2. For the 17.5 and 20 mg/kg L-NMMA doses, docetaxel will be administered at 100 mg/m2. In the Phase II portion of the study, the starting dose will be the RP2D determined in the Phase Ib portion of the study. In the phase II portion of the study, patients will be treated with L-NMMA and taxane (docetaxel, paclitaxel, or nab-paclitaxel) per physician's choice. Patients will be treated with L-NMMA and taxane chemotherapy (docetaxel, paclitaxel, or nab-paclitaxel) per physician's choice. L-NMMA will be administered on Days 1-5 and taxane chemotherapy on Day 1 Q3W or Day 1 Q1W. L-NMMA and docetaxel will be administered at the RP2D determined in the phase Ib portion of the study. Paclitaxel at 175 mg/m2 will be IV infused over 3 hours or 80 mg/m2 will be IV infused over 1 hour, and nab-paclitaxel at 260 mg/m2 will be IV infused over 30 minutes. For L-NMMA-induced hypertension, amlodipine (10 mg) and enteric-coated low-dose aspirin (81 mg) will be orally administered. Amlodipine will be administered for 6 days at each cycle, starting 24 hours before the first dose of L-NMMA. Enteric-coated low-dose aspirin will be administered once daily during the 6 21-day cycles. For docetaxel-induced leukopenia, pegfilgrastim (6 mg) will be administered via subcutaneous injection approximately 24 hours after every dose of docetaxel.

Conditions

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Metastatic Triple Negative Breast Cancer

Keywords

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breast cancer nitric oxide synthase docetaxel L-NMMA

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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L-NMMA 7.5 mg/kg and Docetaxel 75 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 7.5 mg/kg (starting dose) will be administered IV on Days 1-5. Docetaxel will be administered at 75 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

L-NMMA 10 mg/kg and Docetaxel 75 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 10 mg/kg will be administered IV on Days 1-5. Docetaxel will be administered at 75 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

L-NMMA 12.5 mg/kg and Docetaxel 75 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 12.5 mg/kg will be administered IV on Days 1-5. Docetaxel will be administered at 75 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

L-NMMA 15 mg/kg and Docetaxel 75 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 15 mg/kg will be administered IV on Days 1-5. Docetaxel will be administered at 75 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

L-NMMA 17.5 mg/kg and Docetaxel 100 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 17.5 mg/kg will be administered IV on Days 1-5. Docetaxel will be administered at 100 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

L-NMMA 20 mg/kg and Docetaxel 100 mg/m2

Phase Ib: L-NMMA and docetaxel will be given for 6 21-day cycles. L-NMMA at doses of 20 mg/kg will be administered IV on Days 1-5. Docetaxel will be administered at 100 mg/m2 as an IV 15 min after L-NMMA infusion Day 1.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

Phase II: RP2D determined in the Phase Ib

Phase II: L-NMMA starting dose will be the RP2D determined in the Phase Ib portion of the study.

Group Type EXPERIMENTAL

L-NMMA

Intervention Type DRUG

Nitric oxide synthase inhibitor

Docetaxel

Intervention Type DRUG

Mitotic inhibitor, cytotoxic

Amlodipine

Intervention Type DRUG

Long-acting calcium channel blocker

Pegfilgrastim

Intervention Type DRUG

Colony-stimulating factor

Enteric-coated aspirin

Intervention Type DRUG

non-steroidal anti-inflammatory drug

Interventions

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L-NMMA

Nitric oxide synthase inhibitor

Intervention Type DRUG

Docetaxel

Mitotic inhibitor, cytotoxic

Intervention Type DRUG

Amlodipine

Long-acting calcium channel blocker

Intervention Type DRUG

Pegfilgrastim

Colony-stimulating factor

Intervention Type DRUG

Enteric-coated aspirin

non-steroidal anti-inflammatory drug

Intervention Type DRUG

Other Intervention Names

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NG-monomethyl-l-arginine TAXOTERE besylate salt of amlodipine; NORVASC NEULASTA acetylsalicylic acid

Eligibility Criteria

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Inclusion Criteria

Patient must meet all of the following criteria:

• Female patients with pathologically determined advanced (progressive disease or refractory to 3 cycles of standard chemotherapy) or metastatic (any line) triple negative breast cancer (TNBC). TNBC is defined as: Estrogen receptor negative and progesterone receptor negative (\<10% staining by immunohistochemistry \[IHC\]).

Human epidermal growth factor receptor 2 (HER2) negative. HER2 negativity must be confirmed by one of the following:

* Fluorescence in situ hybridization (FISH)-negative (FISH ratio \<2), or
* IHC 0-1+, or
* IHC 2+ AND FISH-negative (FISH ratio \<2). Eastern Cooperative Oncology Group performance status of ≤ 2

* Age ≥ 18 years
* Laboratory values within the following ranges:
* Hemoglobin ≥9.0 g/dL (transfusions permitted)
* Absolute neutrophil count ≥1500/mm3 (1.5 x 109/L)
* Platelet count ≥100,000/mm3 (100 x 109/L)
* Total bilirubin \<2 X upper limit of normal (ULN)
* Creatinine (Cr) \<2 X ULN and Cr clearance (CrCl) ≥30 by Cockcroft and Gault
* Alanine transaminase (ALT) and aspartate transaminase (AST) \<2 X ULN (if liver metastases are present then ALT and AST must be \<5 X ULN)

* Have adequate organ function (cardiac ejection fraction of ≥ 45%)
* Negative serum pregnancy test within 7 days of the administration of the first treatment dose for women of childbearing potential (WOCBP). For WOCBP, adequate contraception must be used throughout the study.
* Ability to understand the requirements of the study, provide written informed consent and authorization of use and disclosure of protected health information, and agree to abide by the study restrictions and return for the required assessments.
* Patient must be willing to undergo biopsies as required by the study protocol. Biopsies will be based on acceptable clinical risks as judged by investigator. Tissue from a previous biopsy will be accepted in the form of tissue slides.

Exclusion Criteria

History of poorly controlled hypertension (defined as systolic blood pressure \>150 mmHg at baseline)

* Patients with metastatic disease who have received radiation therapy, chemotherapy, or non-cytotoxic investigational agents within 2 weeks of study treatment initiation.
* Patients who received docetaxel at any line of treatment within the past 12 months
* Evidence of New York Heart Association class III or greater cardiac disease
* History of myocardial infarction, stroke, ventricular arrhythmia, or symptomatic conduction abnormality within the past 12 months
* History of congenital QT prolongation
* Absolute corrected QT interval of \>480 msec in the presence of potassium \>4.0 milliequivalent/L and magnesium \>1.8 mg/dL
* Any medical or psychiatric condition that would prevent informed consent or limit expected survival to less than 4 weeks
* Symptomatic central nervous system metastases
* Pregnant or nursing women
* Hypersensitivity or intolerance to L-NMMA, docetaxel, amlodipine, pegfilgrastim, or their components
* Use of amlodipine or another calcium channel blocker in the past 14 days
* Alcoholism or hepatic disease with the exception of liver metastases
* Severe renal insufficiency (CrCl \<30 mL/min \[Cockcroft and Gault\])
* History of gastrointestinal bleeding, ulceration, or perforation
* Concurrent use of potent cytochrome P450 (CYP)3A4 inhibitors
* Concurrent use of potent CYP3A4 inducers
* Concurrent use of medications that interact with nitrate/nitrites
* Use of an investigational drug within 14 days preceding the first dose of study medication.
* Concurrent use of any complementary or alternative medicines
* Patients with \> Grade 2 neuropathy
* Inability to take aspirin
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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The Methodist Hospital Research Institute

OTHER

Sponsor Role lead

Responsible Party

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Polly A. Niravath, MD

Principal Investigator, Medical Oncologist

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Polly Niravath, M.D.

Role: PRINCIPAL_INVESTIGATOR

Houston Methodist Cancer Center

Locations

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Houston Methodist Hospital

Houston, Texas, United States

Site Status

Countries

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United States

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Other Identifiers

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Pro00011685

Identifier Type: -

Identifier Source: org_study_id