Trial Outcomes & Findings for Safety Study of RMJH-111b to Treat Essential Hypertension (NCT NCT02822222)

NCT ID: NCT02822222

Last Updated: 2020-09-23

Results Overview

Safety \& tolerability were primarily assessed based on the incidence of reported TEAEs by system organ class \& preferred term, as well as by categories: TEAE, severe TEAE, serious TEAE, drug-related TEAE, drug-related severe TEAE, drug-related serious TEAE, TEAE leading to discontinuation, \& TEAE with outcome of death. Drug-related TEAEs were defined as TEAEs assigned a Study Drug (active or placebo) relationship of "adverse reaction" or "suspected adverse reaction". TEAEs were coded using the Medical Dictionary for Regulatory Activities, version 19.0. The severity of TEAEs were graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events, version 4.03. TEAEs were defined as any adverse event that started or increased in severity after the first randomized dose of Study Drug on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).

Recruitment status

COMPLETED

Study phase

PHASE1/PHASE2

Target enrollment

22 participants

Primary outcome timeframe

14 days +/- 3 days

Results posted on

2020-09-23

Participant Flow

This trial was conducted at a single Investigational Site (Orange County Research Center) in Tustin, CA. All subject recruitment procedures and materials were reviewed \& approved by a central IRB prior to their use. The 1st screening visit was May 31, 2016, the 1st subject was enrolled on June 10, 2016, \& the last subject visit was July 7, 2016.

Randomization to treatment took place on Day 4, following the 7-day washout period \& the 3-day run-in placebo period. Only subjects that continued to meet eligibility criteria following the Day 4 baseline procedures were randomized.

Participant milestones

Participant milestones
Measure
RMJH-111b
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Overall Study
STARTED
16
6
Overall Study
COMPLETED
15
6
Overall Study
NOT COMPLETED
1
0

Reasons for withdrawal

Reasons for withdrawal
Measure
RMJH-111b
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Overall Study
Protocol requirement SBP<110 mmHg
1
0

Baseline Characteristics

Safety Study of RMJH-111b to Treat Essential Hypertension

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Total
n=22 Participants
Total of all reporting groups
Sex: Female, Male
Male
8 Participants
n=5 Participants
6 Participants
n=7 Participants
14 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
1 Participants
n=5 Participants
1 Participants
n=7 Participants
2 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
15 Participants
n=5 Participants
5 Participants
n=7 Participants
20 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
9 Participants
n=5 Participants
3 Participants
n=7 Participants
12 Participants
n=5 Participants
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
14 Participants
n=5 Participants
4 Participants
n=7 Participants
18 Participants
n=5 Participants
Age, Categorical
>=65 years
2 Participants
n=5 Participants
2 Participants
n=7 Participants
4 Participants
n=5 Participants
Age, Continuous
58.08 years
STANDARD_DEVIATION 8.882 • n=5 Participants
61.36 years
STANDARD_DEVIATION 5.889 • n=7 Participants
58.98 years
STANDARD_DEVIATION 8.175 • n=5 Participants
Sex: Female, Male
Female
8 Participants
n=5 Participants
0 Participants
n=7 Participants
8 Participants
n=5 Participants
Race (NIH/OMB)
White
5 Participants
n=5 Participants
3 Participants
n=7 Participants
8 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
16 participants
n=5 Participants
6 participants
n=7 Participants
22 participants
n=5 Participants
Height
170.3 cm
STANDARD_DEVIATION 8.79 • n=5 Participants
174.0 cm
STANDARD_DEVIATION 10.43 • n=7 Participants
171.3 cm
STANDARD_DEVIATION 9.16 • n=5 Participants
Weight
90.54 kg
STANDARD_DEVIATION 14.865 • n=5 Participants
89.35 kg
STANDARD_DEVIATION 9.492 • n=7 Participants
90.21 kg
STANDARD_DEVIATION 13.401 • n=5 Participants
Heart rate
72.50 beats per minute
STANDARD_DEVIATION 7.572 • n=5 Participants
66.33 beats per minute
STANDARD_DEVIATION 8.710 • n=7 Participants
70.82 beats per minute
STANDARD_DEVIATION 8.180 • n=5 Participants
Respiration rate
12.75 breaths per minute
STANDARD_DEVIATION 1.000 • n=5 Participants
12.33 breaths per minute
STANDARD_DEVIATION 0.816 • n=7 Participants
12.64 breaths per minute
STANDARD_DEVIATION 0.953 • n=5 Participants
Temperature
36.59 degrees Celsius
STANDARD_DEVIATION 0.208 • n=5 Participants
36.60 degrees Celsius
STANDARD_DEVIATION 0.063 • n=7 Participants
36.60 degrees Celsius
STANDARD_DEVIATION 0.179 • n=5 Participants
Seated SBP
160.4 mmHg
STANDARD_DEVIATION 8.58 • n=5 Participants
162.0 mmHg
STANDARD_DEVIATION 5.06 • n=7 Participants
160.9 mmHg
STANDARD_DEVIATION 7.69 • n=5 Participants
Seated DBP
101.4 mmHg
STANDARD_DEVIATION 5.18 • n=5 Participants
102.0 mmHg
STANDARD_DEVIATION 5.18 • n=7 Participants
101.6 mmHg
STANDARD_DEVIATION 5.06 • n=5 Participants
SBPday
149.8 mmHg
STANDARD_DEVIATION 13.27 • n=5 Participants
147.4 mmHg
STANDARD_DEVIATION 9.61 • n=7 Participants
149.1 mmHg
STANDARD_DEVIATION 12.22 • n=5 Participants
SBPnight
138.0 mmHg
STANDARD_DEVIATION 17.34 • n=5 Participants
133.3 mmHg
STANDARD_DEVIATION 9.31 • n=7 Participants
136.7 mmHg
STANDARD_DEVIATION 15.48 • n=5 Participants
SBP24hr
145.3 mmHg
STANDARD_DEVIATION 14.02 • n=5 Participants
141.2 mmHg
STANDARD_DEVIATION 8.07 • n=7 Participants
144.2 mmHg
STANDARD_DEVIATION 12.62 • n=5 Participants
DBPday
92.6 mmHg
STANDARD_DEVIATION 11.20 • n=5 Participants
93.3 mmHg
STANDARD_DEVIATION 9.81 • n=7 Participants
92.8 mmHg
STANDARD_DEVIATION 10.61 • n=5 Participants
DBPnight
82.3 mmHg
STANDARD_DEVIATION 12.14 • n=5 Participants
82.6 mmHg
STANDARD_DEVIATION 3.23 • n=7 Participants
82.4 mmHg
STANDARD_DEVIATION 10.38 • n=5 Participants
DBP24hr
88.6 mmHg
STANDARD_DEVIATION 10.91 • n=5 Participants
88.8 mmHg
STANDARD_DEVIATION 6.04 • n=7 Participants
88.7 mmHg
STANDARD_DEVIATION 9.69 • n=5 Participants
Total Serum Magnesium AUC
41.4 mEq*24hr/L
STANDARD_DEVIATION 2.42 • n=5 Participants
42.8 mEq*24hr/L
STANDARD_DEVIATION 2.76 • n=7 Participants
41.8 mEq*24hr/L
STANDARD_DEVIATION 2.53 • n=5 Participants
Total Serum Magnesium Concentration
1.75 mEq/L
STANDARD_DEVIATION 0.103 • n=5 Participants
1.78 mEq/L
STANDARD_DEVIATION 0.117 • n=7 Participants
1.76 mEq/L
STANDARD_DEVIATION 0.105 • n=5 Participants
24-hour urinary magnesium excretion
7.3 mEq/24 hrs
STANDARD_DEVIATION 2.59 • n=5 Participants
7.0 mEq/24 hrs
STANDARD_DEVIATION 4.34 • n=7 Participants
7.2 mEq/24 hrs
STANDARD_DEVIATION 3.05 • n=5 Participants
12-lead ECG Ventricular Rate
70.31 bpm
STANDARD_DEVIATION 9.904 • n=5 Participants
65.83 bpm
STANDARD_DEVIATION 14.851 • n=7 Participants
69.09 bpm
STANDARD_DEVIATION 11.258 • n=5 Participants
12-Lead ECG Intervals
RR Interval
863.31 msec
STANDARD_DEVIATION 113.781 • n=5 Participants
944.17 msec
STANDARD_DEVIATION 199.272 • n=7 Participants
885.36 msec
STANDARD_DEVIATION 141.635 • n=5 Participants
12-Lead ECG Intervals
PR Interval
168.38 msec
STANDARD_DEVIATION 18.143 • n=5 Participants
155.67 msec
STANDARD_DEVIATION 10.985 • n=7 Participants
164.91 msec
STANDARD_DEVIATION 17.246 • n=5 Participants
12-Lead ECG Intervals
QRS Interval
90.00 msec
STANDARD_DEVIATION 9.033 • n=5 Participants
97.67 msec
STANDARD_DEVIATION 19.159 • n=7 Participants
92.09 msec
STANDARD_DEVIATION 12.566 • n=5 Participants
12-Lead ECG Intervals
QT Interval
398.56 msec
STANDARD_DEVIATION 24.916 • n=5 Participants
416.00 msec
STANDARD_DEVIATION 48.233 • n=7 Participants
403.32 msec
STANDARD_DEVIATION 32.566 • n=5 Participants
12-Lead ECG Intervals
Corrected QT Interval
416.25 msec
STANDARD_DEVIATION 21.592 • n=5 Participants
424.00 msec
STANDARD_DEVIATION 23.639 • n=7 Participants
418.36 msec
STANDARD_DEVIATION 21.875 • n=5 Participants
12-Lead ECG Diagnosis
Normal
9 Participants
n=5 Participants
3 Participants
n=7 Participants
12 Participants
n=5 Participants
12-Lead ECG Diagnosis
Abnormal NCS
7 Participants
n=5 Participants
3 Participants
n=7 Participants
10 Participants
n=5 Participants
12-Lead ECG Diagnosis
Abnormal CS
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Patellar Reflex Score
2+
11 Participants
n=5 Participants
6 Participants
n=7 Participants
17 Participants
n=5 Participants
Patellar Reflex Score
1+
5 Participants
n=5 Participants
0 Participants
n=7 Participants
5 Participants
n=5 Participants

PRIMARY outcome

Timeframe: 14 days +/- 3 days

Population: Safety population = all randomized subjects who received at least one dose of Study Drug (active or placebo).

Safety \& tolerability were primarily assessed based on the incidence of reported TEAEs by system organ class \& preferred term, as well as by categories: TEAE, severe TEAE, serious TEAE, drug-related TEAE, drug-related severe TEAE, drug-related serious TEAE, TEAE leading to discontinuation, \& TEAE with outcome of death. Drug-related TEAEs were defined as TEAEs assigned a Study Drug (active or placebo) relationship of "adverse reaction" or "suspected adverse reaction". TEAEs were coded using the Medical Dictionary for Regulatory Activities, version 19.0. The severity of TEAEs were graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events, version 4.03. TEAEs were defined as any adverse event that started or increased in severity after the first randomized dose of Study Drug on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
TEAE
5 Participants
1 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Severe TEAE
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Serious TEAE
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Drug-related TEAE
2 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Drug-related severe TEAE
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Drug-related serious TEAE
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
TEAE leading to discontinuation from study
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
TEAE with outcome of death
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Mild abdominal pain upper
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Moderate constipation
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Mild diarrhoea
2 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Mild flatulence
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Mild headache
1 Participants
1 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Treatment Emergent Adverse Events (TEAEs)
Mild cough
2 Participants
0 Participants

PRIMARY outcome

Timeframe: 8 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 10-11 assessment (i.e., n=15).

Change in the mean value of 24-hour urinary magnesium excretion from baseline (Day 3 to pre-dose Day 4) to end of treatment (Day 10 to Day 11). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 24-hour Urinary Magnesium Excretion
9.1 mEq/24 hours
Standard Deviation 5.44
-1.3 mEq/24 hours
Standard Deviation 5.57

PRIMARY outcome

Timeframe: 7 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 11 assessments (i.e., n=15).

Changes in the mean values for seated SBP \& DBP from baseline (pre-dose Day 4) to end of treatment (Day 11). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Seated SBP and DBP
Change in seated SBP
-14.6 mmHg
Standard Deviation 13.58
-7.2 mmHg
Standard Deviation 7.36
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Seated SBP and DBP
Change in seated DBP
-6.4 mmHg
Standard Deviation 6.08
-2.3 mmHg
Standard Deviation 6.25

PRIMARY outcome

Timeframe: 10 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 11 assessment (i.e., n=15).

Change in mean body weight from baseline (Day 1) to end of treatment (Day 11). The weight measurements were made using a calibrated scale with the subject wearing light clothes and no shoes. Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Body Weight
-1.09 kg
Standard Deviation 2.372
-0.79 kg
Standard Deviation 1.421

PRIMARY outcome

Timeframe: Up to 6 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug

Change in mean 12-lead ECG ventricular rate from baseline (Screening or Day 1) to 3 hours after the first randomized dose (Day 4). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. The ECG assessments were recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Ventricular Rate (3 Hours After 1st Dose)
-0.31 bpm
Standard Deviation 8.554
-5.17 bpm
Standard Deviation 6.735

PRIMARY outcome

Timeframe: Up to 13 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 11 assessment (i.e., n=15).

Change in mean 12-lead ECG ventricular rate from baseline (Screening or Day 1) to after the last randomized dose (Day 11). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. The ECG assessments were recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Ventricular Rate (After Last Dose)
4.73 bpm
Standard Deviation 10.579
-3.83 bpm
Standard Deviation 8.519

PRIMARY outcome

Timeframe: Up to 6 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug

Change in mean 12-lead ECG intervals (RR interval, PR interval, QRS interval, QT interval, corrected QT interval based on Fridericia formula) from baseline (Screening or Day 1) to 3 hours after the first randomized dose (Day 4). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. The ECG assessments were recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (3 Hours After 1st Dose)
Change in RR Interval
8.06 msec
Standard Deviation 102.179
55.33 msec
Standard Deviation 96.804
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (3 Hours After 1st Dose)
Change in PR Interval
0.75 msec
Standard Deviation 13.364
8.67 msec
Standard Deviation 4.502
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (3 Hours After 1st Dose)
Change in QRS Interval
-2.63 msec
Standard Deviation 4.113
2.33 msec
Standard Deviation 4.274
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (3 Hours After 1st Dose)
Change in QT Interval
-5.06 msec
Standard Deviation 26.727
-0.33 msec
Standard Deviation 23.372
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (3 Hours After 1st Dose)
Change in Corrected QT Interval
-5.06 msec
Standard Deviation 16.097
-9.17 msec
Standard Deviation 16.005

PRIMARY outcome

Timeframe: Up to 13 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 11 assessment (i.e., n=15).

Change in mean 12-lead ECG intervals (RR interval, PR interval, QRS interval, QT interval, corrected QT interval based on Fridericia formula) from baseline (Screening or Day 1) to after the last randomized dose (Day 11). Mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. The ECG assessments were recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (After Last Dose)
Change in RR Interval
-57.00 msec
Standard Deviation 122.412
29.67 msec
Standard Deviation 82.921
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (After Last Dose)
Change in PR Interval
-4.00 msec
Standard Deviation 12.806
8.33 msec
Standard Deviation 13.530
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (After Last Dose)
Change in QRS Interval
-1.33 msec
Standard Deviation 3.677
-0.67 msec
Standard Deviation 5.317
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (After Last Dose)
Change in QT Interval
-11.67 msec
Standard Deviation 24.732
5.67 msec
Standard Deviation 22.393
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Intervals (After Last Dose)
Change in Corrected QT Interval
-1.47 msec
Standard Deviation 11.332
-0.33 msec
Standard Deviation 12.307

PRIMARY outcome

Timeframe: Up to 6 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug

Change in 12-lead ECG diagnosis \[i.e., normal to abnormal not clinically significant (NCS); normal to abnormal clinically significant (CS); abnormal NCS to abnormal CS; remained abnormal NCS; remained normal\] from baseline (Screening or Day 1) to 3 hours after the first randomized dose (Day 4). The ECG was recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (3 Hours After 1st Dose)
Changed from Normal to Abnormal NCS
1 Participants
1 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (3 Hours After 1st Dose)
Changed from Normal to Abnormal CS
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (3 Hours After 1st Dose)
Changed from Abnormal NCS to Abnormal CS
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (3 Hours After 1st Dose)
Remained Abnormal NCS
7 Participants
3 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (3 Hours After 1st Dose)
Remained Normal
8 Participants
2 Participants

PRIMARY outcome

Timeframe: Up to 13 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 subject in RMJH-111b arm was discontinued on Day 9 due to protocol-specified criterion \[SBP\<110 mmHg; the SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs\] \& is therefore missing Day 11 assessment (i.e., n=15).

Change in 12-lead ECG diagnosis \[i.e., normal to abnormal not clinically significant (NCS); normal to abnormal clinically significant (CS); abnormal NCS to abnormal CS; remained abnormal NCS; remained normal\] from baseline (Screening or Day 1) to after the last randomized dose (Day 11). The ECG was recorded after the subject was resting at least 5 minutes in the supine position in a quiet environment.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Changed from Normal to Abnormal NCS
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Changed from Normal to Abnormal CS
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Changed from Abnormal NCS to Abnormal CS
0 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Remained Abnormal NCS
6 Participants
2 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Remained Normal
7 Participants
3 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in 12-lead ECG Diagnosis (After Last Dose)
Changed from Abnormal NCS to Normal
1 Participants
1 Participants

PRIMARY outcome

Timeframe: 14 days +/- 3 days

Population: Safety population = all randomized subjects who received at least 1 dose of Study Drug. 1 RMJH-111b subject was discontinued on Day 9 due to protocol requirement \[SBP\<110 mmHg; SBPs (102-109 mmHg) not associated with clinical symptoms \& not assigned as TEAEs\] \& lacked Day 10-11 assessments (i.e., n=15). Subject was at 2+ for Day 4-9 \& Final Visit.

Changes in patellar reflex score from baseline (pre-dose Day 4) to interim time points during the randomized treatment period (prior to each morning dose on Days 5 through 10) and to post-dose time points (Day 11 \& Day 18 ± 3 days). The scoring values included 0, 1+, 2+, 3+, and 4+, where 2+ means normal patellar reflex and lower scores indicate a worsened outcome (1+ means reflex present only with reinforcement and 0 means loss of reflex). The patellar reflex assessment was made with the subject in the seated position, with legs hanging freely from the exam table. Loss of patellar reflex is an early sign of magnesium toxicity, and thus the test serves as an assessment for functional magnesium status. A clinical indication of a safe magnesium dosage regimen included the presence of the patellar reflex (knee jerk).

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained at 2+
9 Participants
6 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained at 1+
2 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained at 2+ except Day 9 (1+)
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained at 1+ except Day 5 & 8 (2+)
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained at 1+ except Day 18 (2+)
1 Participants
0 Participants
Safety and Tolerability of RMJH-111b Compared to Placebo Based on Change in Patellar Reflex Score
Remained 1+ except Days 6, 10, & 11 (2+)
1 Participants
0 Participants

SECONDARY outcome

Timeframe: 8 days

Population: PK population = all randomized subjects who received at least 1 dose of Study Drug with valid data for at least 1 pre- \& post-dose assessment. 1 subject in RMJH-111b arm was missing PK assessments beyond Day 8, \& thus is only included in the analyses for Days 3 \& 4 and not for Day 10 (i.e., n = 16 for Days 3 \& 4, but n = 15 for Day 10).

Venous blood samples were collected within 1 hour prior to \& 0.5, 1, 2, 3, 6, \& 12 hours following the morning dose of Study Drug on Days 3 (run-in placebo) and 4, \& within 1 hour prior to \& 0.5, 1, 2, 3, 6, 12, \& 24 hours following the morning dose of Study Drug on Day 10. Blood samples were processed to serum \& assayed for total serum magnesium by a central laboratory. The LLOQ was 0.06 mEq/L. Individual PK parameters for Days 4 \& 10 were to be calculated using corrected concentration-time curves (with individual's baseline assessments of endogenous total serum magnesium at Day 3 subtracted); however, due to small numerical values after correction, the planned PK parameters could not be derived. The analyses were reattempted using the observed values (that include the endogenous levels of magnesium), which allowed AUC0-24 to be derived.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on AUC
Day 3 (pre-dose)
41.4 mEq*24hr/L
Standard Deviation 2.42
42.8 mEq*24hr/L
Standard Deviation 2.76
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on AUC
Day 4 (1st day of dosing)
43.9 mEq*24hr/L
Standard Deviation 2.53
42.4 mEq*24hr/L
Standard Deviation 3.03
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on AUC
Day 10 (7th day of dosing)
43.9 mEq*24hr/L
Standard Deviation 1.87
41.7 mEq*24hr/L
Standard Deviation 2.52

SECONDARY outcome

Timeframe: 6 days

Population: PK population = all randomized subjects who received at least 1 dose of Study Drug with valid data for at least 1 pre- \& post-dose assessment. 1 subject in RMJH-111b arm was missing PK assessments beyond Day 8, \& thus is only included in the analyses for Days 5-8 and not for Days 9-10 (i.e., n = 16 for Days 5-8, but n = 15 for Days 9-10).

Venous blood samples were collected within 1 hour prior to the morning dose of Study Drug (active or placebo) on Days 4, 5, 6, 7, 8, 9, and 10. Blood samples were processed to serum \& assayed for total serum magnesium by a central laboratory (LLOQ = 0.06 mEq/L). Ratios of the individual total serum magnesium trough concentrations at Days 5, 6, 7, 8, 9, and 10 relative to baseline (pre-dose Day 4) were calculated. Observed concentrations (including the endogenous levels of magnesium) were used for this purpose.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 5/Day 4 trough concentrations
1.09 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.059
1.00 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.034
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 6/Day 4 trough concentrations
1.10 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.071
0.99 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.041
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 7/Day 4 trough concentrations
1.08 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.097
0.98 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.045
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 8/Day 4 trough concentrations
1.09 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.087
1.01 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.043
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 9/Day 4 trough concentrations
1.06 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.063
1.02 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.058
Pharmacokinetics of Total Serum Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on Trough Concentration Ratio
Day 10/Day 4 trough concentrations
1.02 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.080
0.96 unitless (i.e., ratio of mEq/L to mEq/L)
Standard Deviation 0.028

SECONDARY outcome

Timeframe: 8 days

Population: PK population = all randomized subjects who received at least 1 dose of Study Drug with valid data for at least 1 pre- \& post-dose assessment. 1 subject in RMJH-111b arm was missing PK assessments beyond Day 8, \& thus is only included in the analyses for Days 3 \& 4 and not for Day 10 (i.e., n = 16 for Days 3 and 4, but n = 15 for Day 10).

Urinary excretion of magnesium was assessed over three 24-hour periods. The first urine collection started immediately after the morning dose of run-in placebo on Day 3, and the second and third collections started immediately after the morning dose of Study Drug on Days 4 and 10, respectively. Observed 24-hour urinary magnesium excretion values at Days 3, 4, and 10 (i.e., without subtraction of the Day 3 values to correct for the individual's baseline assessment of endogenous urinary magnesium excretion) were used for comparisons with the total serum magnesium data (as only the observed serum values allowed for PK parameter derivation).

Outcome measures

Outcome measures
Measure
RMJH-111b
n=16 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Pharmacokinetics of Urine Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on 24-hour Urinary Excretion
Day 3 (pre-dose)
7.3 mEq/24 hrs
Standard Deviation 2.59
7.0 mEq/24 hrs
Standard Deviation 4.34
Pharmacokinetics of Urine Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on 24-hour Urinary Excretion
Day 4 (1st day of dosing)
9.8 mEq/24 hrs
Standard Deviation 3.90
5.7 mEq/24 hrs
Standard Deviation 1.97
Pharmacokinetics of Urine Magnesium After Single and Repeated Oral Administrations of RMJH-111b Compared to Placebo Based on 24-hour Urinary Excretion
Day 10 (7th day of dosing)
16.5 mEq/24 hrs
Standard Deviation 6.23
5.7 mEq/24 hrs
Standard Deviation 1.63

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

SBPday = mean daytime (8 AM to 4 PM) ABPM SBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. SBPday was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in SBPday After 7 Days of Treatment
-4.6 mmHg
Standard Deviation 11.72
1.6 mmHg
Standard Deviation 11.36

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

SBPnight = mean daytime (10 PM to 6 AM) ABPM SBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. SBPnight was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in SBPnight After 7 Days of Treatment
-4.2 mmHg
Standard Deviation 12.54
0.7 mmHg
Standard Deviation 12.13

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

SBP24hr = mean 24-hour ABPM SBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. SBP24hr was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in SBP24hr After 7 Days of Treatment
-3.8 mmHg
Standard Deviation 11.72
2.3 mmHg
Standard Deviation 11.61

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

DBPday = mean daytime (8 AM to 4 PM) ABPM DBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. DBPday was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in DBPday After 7 Days of Treatment
-3.5 mmHg
Standard Deviation 6.88
-1.2 mmHg
Standard Deviation 7.12

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

DBPnight = mean nighttime (10 PM to 6 AM) ABPM DBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. DBPnight was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in DBPnight After 7 Days of Treatment
-1.9 mmHg
Standard Deviation 8.76
-0.6 mmHg
Standard Deviation 7.46

SECONDARY outcome

Timeframe: 8 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& the Day 3-Day 4 and Day 10-Day 11 ABPM assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 due to meeting protocol-specified criterion (SBP \< 110 mmHg). The SBPs (102-109 mmHg) were not associated with clinical symptoms \& were not assigned as TEAEs.

DBP24hr = mean 24-hour ABPM DBP. Note, mean values of change that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase. DBP24hr was measured twice during the subject's stay at the CRU: baseline measurements were collected from Day 3 to pre-dose Day 4 \& post-dose measurements were collected from Day 10 to Day 11. An Investigational Site staff member placed the cuff on the subject's non-dominant arm \& connected the ABPM at approximately 7 AM (± 60 minutes) on Days 3 \& 10. A pre-dose ABPM reading was recorded immediately prior to the morning dose of run-in period placebo on Day 3 \& the morning dose of treatment period Study Drug (active or placebo) on Day 10. The ABPM was started again immediately after dosing. The ABPM was removed at approximately 8 AM (± 60 minutes) on Days 4 and 11, respectively, but no sooner than 24 hours after the continuous monitoring was initiated.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in DBP24hr After 7 Days of Treatment
-2.3 mmHg
Standard Deviation 6.97
0.3 mmHg
Standard Deviation 6.53

SECONDARY outcome

Timeframe: 7 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& pre-dose Day 4 \& Day 11 assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 for meeting protocol-specified criterion \[SBP\<110 mmHg; SBPs (102-109 mmHg) were not associated with clinical symptoms \& not assigned as TEAEs\] \& is therefore excluded.

Change from baseline (pre-dose Day 4) in seated SBP after 7 days of treatment (Day 11) with RMJH-111b compared to placebo was a secondary efficacy variable (i.e., seated SBP served dual functions as safety and efficacy variables, with the safety population used for the safety analyses described in outcome 3 and the efficacy population used for the efficacy analyses described here). Note, mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in Seated SBP After 7 Days of Treatment
-14.6 mmHg
Standard Deviation 13.58
-7.2 mmHg
Standard Deviation 7.36

SECONDARY outcome

Timeframe: 7 days

Population: Efficacy population = all randomized subjects who completed 7 days of treatment \& pre-dose Day 4 \& Day 11 assessments. 1 subject in RMJH-111b arm was discontinued on Day 9 for meeting protocol-specified criterion \[SBP\<110 mmHg; SBPs (102-109 mmHg) were not associated with clinical symptoms \& not assigned as TEAEs\] \& is therefore excluded.

Change from baseline (pre-dose Day 4) in seated DBP after 7 days of treatment (Day 11) with RMJH-111b compared to placebo was a secondary efficacy variable (i.e., seated DBP served dual functions as safety and efficacy variables, with the safety population used for the safety analyses described in outcome 3 and the efficacy population used for the efficacy analyses described here). Note, mean values that are negative represent a decrease in that value from baseline, whereas values that are positive represent an increase.

Outcome measures

Outcome measures
Measure
RMJH-111b
n=15 Participants
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 Participants
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Efficacy of RMJH-111b Compared to Placebo Based on Change From Baseline in Seated DBP After 7 Days of Treatment
-6.4 mmHg
Standard Deviation 6.08
-2.3 mmHg
Standard Deviation 6.25

Adverse Events

RMJH-111b

Serious events: 0 serious events
Other events: 5 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
RMJH-111b
n=16 participants at risk
Four (4) RMJH-111b (magnesium citrate, tribasic anhydrous) soft gelatin capsules (110 mg elemental magnesium/capsule) orally bid for 7 days
Placebo
n=6 participants at risk
Four (4) placebo soft gelatin capsules (0 mg elemental magnesium/capsule) orally bid for 7 days
Gastrointestinal disorders
Abdominal pain upper
6.2%
1/16 • Number of events 1 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
0.00%
0/6 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
Gastrointestinal disorders
Constipation
6.2%
1/16 • Number of events 1 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
0.00%
0/6 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
Gastrointestinal disorders
Diarrhoea
12.5%
2/16 • Number of events 2 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
0.00%
0/6 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
Gastrointestinal disorders
Flatulence
6.2%
1/16 • Number of events 1 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
0.00%
0/6 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
Nervous system disorders
Headache
6.2%
1/16 • Number of events 1 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
16.7%
1/6 • Number of events 2 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
Respiratory, thoracic and mediastinal disorders
Cough
12.5%
2/16 • Number of events 2 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.
0.00%
0/6 • 14 +/- 3 days. Treatment emergent adverse events (TEAEs) were defined as any adverse event (AE) that started or increased in severity after the first randomized dose of Study Drug (active or placebo) on Day 4 through the Final Study Visit (8 +/-3 days after last randomized dose).
AE = any untoward medical occurrence associated with use of Study Drug (active or placebo), whether or not considered drug related. Includes any unfavorable \& unintended sign (e.g., an abnormal laboratory finding), symptom, or disease temporally associated with the use of the drug, \& does not imply any judgment about causality. Can arise with any use of the drug (e.g., off-label use, use in combination with another drug) \& any route of administration, formulation, or dose, including overdose.

Additional Information

Russell Jaffe, M.D., Chief Executive Officer

RMJ Holdings LLC d/b/a RMJH Rx

Phone: (703) 840-4440

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place