Analyses of Bone Marrow and Blood Samples From Healthy Volunteers: Focus on the redOx Metabolism
NCT ID: NCT02789839
Last Updated: 2025-12-30
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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COMPLETED
NA
102 participants
INTERVENTIONAL
2016-08-31
2017-08-31
Brief Summary
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The level of ROS in cells is the result of production (mainly the NADPHoxydase and the mitochondrial respiratory chain) and their disposal via antioxidant enzymes. The hematology team of the university hospital of Tours (France) found that certain antioxidant enzymes are essential for the self-renewal of normal and leukemic cells such as glutathione peroxidase-3 (Herault O et al, J Exp Med 2012, 209:895). Their expression is modified in hematological diseases (patent WO2012085188A1 / 2012-06-28 and FR1000267310 / 2014-11-27).
Age potentially affects the level of expression of antioxidant genes, since it was established a decrease in glutathione peroxidase activity in women over 65 years (Sara E, J Gerontol A Biol Sci Med Sci 2008 63: 505). The study HEALTHOX will determine the impact of age on the oxidative metabolism of normal hematopoietic cells, and in particular the expression of antioxidant genes. It will provide a reference for analyzing disturbances of oxidative metabolism in blood diseases. The main objective is the comparison of peripheral blood and bone marrow cells depending from 3 groups of healthy volunteers: 18-39 years, 40-59 years and 60-85 years. The level of ROS and the expression of genes encoding the major antioxidant enzymes involved in the regulation of oxidative stress will be studied. The secondary objectives are to create a cell bank (blood and marrow), a blood plasma bank and a cDNA library (blood and marrow) from healthy volunteers.
Detailed Description
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Conditions
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Study Design
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NA
SINGLE_GROUP
BASIC_SCIENCE
NONE
Study Groups
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Healthy volunteer
Blood test Medullar test
Blood test
Blood test of 30mL
Medullar test
Medullar test for 2-5mL and a smear
Interventions
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Blood test
Blood test of 30mL
Medullar test
Medullar test for 2-5mL and a smear
Eligibility Criteria
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Inclusion Criteria
* Age ≥ 18 years old and ≤ 85 ans
* Informed and written consent
* Affiliation to Social Security
Exclusion Criteria
* Subject with an history of tumoral disease with chemotherapy or radiotherapy
* Subject with an history of infectious or inflammatory disease
* Subject taking a treatment in prevention of blood clots
* Subject under guardianship or protection measure
* Subject with in the last 30 days food supplement rich in antioxidant : ascorbic acid, trace elements, etc…
* Subject with in the last 30 days treatment with known effect on oxydative metabolism : N-Acetyl-Cysteine, etc…
* Participant to a drug study
* Pregnant woman
18 Years
85 Years
ALL
Yes
Sponsors
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University Hospital, Tours
OTHER
Responsible Party
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Principal Investigators
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Olivier HERAULT, PhD
Role: PRINCIPAL_INVESTIGATOR
CHRU TOURS
Locations
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CHRU Tours
Tours, , France
Countries
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References
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Guermouche H, Ravalet N, Gallay N, Deswarte C, Foucault A, Beaud J, Rault E, Saindoy E, Lachot S, Martignoles JA, Gissot V, Suner L, Gyan E, Delhommeau F, Herault O, Hirsch P. High prevalence of clonal hematopoiesis in the blood and bone marrow of healthy volunteers. Blood Adv. 2020 Aug 11;4(15):3550-3557. doi: 10.1182/bloodadvances.2020001582.
Other Identifiers
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PHAO 2016 - OH / HEALTHOX
Identifier Type: -
Identifier Source: org_study_id