A Safety and Immunogenicity Trial of IHV01

NCT ID: NCT02756208

Last Updated: 2021-12-16

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

65 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-11-30

Study Completion Date

2018-07-31

Brief Summary

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This study is designed to evaluate the safety of the FLSC vaccine and will be a randomized, placebo-controlled, modified double-blinded dose escalation study in 60 healthy adult volunteers (Human Immunodeficiency Virus-1 uninfected).

Detailed Description

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This is a Phase 1 randomized, placebo-controlled, modified double-blinded dose escalation study in 60 healthy adult volunteers who are Human Immunodeficiency Virus-1 (HIV-1) uninfected. Participants in the study will receive 4 injections at 0, 4, 8 and 24 weeks and will be followed for an additional 24 weeks. The total study duration will be 48 weeks. As this is a Phase 1 trial, the primary objective is to document safety of the Full Length Single Chain (FLSC) gp120-CD4 complex vaccine with a secondary objective to evaluate immune responses induced by the vaccine. This vaccine is being evaluated as it is constructed so that the gp120 and CD4 moieties form a stable intra-chain binding interaction that forms a transition state structure that presents conserved, conformational domains involved in the early HIV replication process. It is hypothesized that antibodies directed to these epitopes would be highly cross-reactive and potentially useful for HIV vaccine development.

Conditions

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HIV Infection

Keywords

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vaccine healthy volunteer

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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300 ug FLSC vaccine

Subjects will be vaccinated with 300 ug FLSC vaccine (highest vaccine dose) on study days 0, 28, 56 and 168.

Group Type EXPERIMENTAL

300 ug FLSC vaccine

Intervention Type BIOLOGICAL

FLSC vaccine 300 ug (1.0 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

150 ug FLSC vaccine

Subjects will be vaccinated with 150 ug FLSC vaccine (middle vaccine dose) on study days 0, 28, 56 and 168.

Group Type EXPERIMENTAL

150 ug FLSC vaccine

Intervention Type BIOLOGICAL

FLSC vaccine 150 ug (0.5 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

75 ug FLSC vaccine

Subjects will be vaccinated with 75 ug FLSC vaccine (lowest vaccine dose) on study days 0, 28, 56 and 168.

Group Type EXPERIMENTAL

75 ug FLSC vaccine

Intervention Type BIOLOGICAL

FLSC vaccine 75 ug (0.25 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Placebo

Subjects will be vaccinated with placebo (control group) on study days 0, 28, 56 and 168.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Placebo sterile saline (0.25 - 1.0 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Interventions

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300 ug FLSC vaccine

FLSC vaccine 300 ug (1.0 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Intervention Type BIOLOGICAL

150 ug FLSC vaccine

FLSC vaccine 150 ug (0.5 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Intervention Type BIOLOGICAL

75 ug FLSC vaccine

FLSC vaccine 75 ug (0.25 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Intervention Type BIOLOGICAL

Placebo

Placebo sterile saline (0.25 - 1.0 mL) given by intramuscular injection into the arm on study Days 0, 28, 56, 168

Intervention Type BIOLOGICAL

Other Intervention Names

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Full length single chain gp120-CD4 complex vaccine Full length single chain gp120-CD4 complex vaccine Full length single chain gp120-CD4 complex vaccine sterile saline

Eligibility Criteria

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Inclusion Criteria

1. Age: 18 to 45 years of age.
2. Sex: Male or Female (female volunteers of child bearing potential must have a negative serum beta human chorionic gonadotropin (b-HCG or pregnancy) test at time of screening and entry into the study and provide assurance of the use of effective(as judged by the investigator) birth control methods or abstinence beginning at least 60 days prior to the study and during the study
3. Documented HIV-1 negative by ELISA

Exclusion Criteria

5. No identifiable risk factor for acquisition of HIV infection (i.e., intravenous drug use/needle sharing, unprotected sex with multiple partners)
6. Negative b-HCG pregnancy test on the day of initial vaccination.
7. Negative screen for Hepatitis B surface antigen (HBsAg);
8. Negative screen for antibodies to Hepatitis C virus (Patient may enroll if patient can provide documentation of negative hepatitis C viral load.)
9. Participant must have a CD4 count ( a type of white blood cells) within the normal range of the clinical laboratory utilized for the study and a CD4 percentage within 20% of the normal range of the clinical laboratory
11. Volunteers must be willing and able to provide written informed consent to participate in the study.
12. Available for at least 48 weeks of follow-up.


1. High risk behavior for acquisition of HIV within 24 weeks of study entry(i.e., intravenous drug use/needle sharing, unprotected sex with multiple partners)
2. Volunteers with an acute and clinically significant medical event (as determined by the investigator) within the past 30 days of screening.
3. Have active tuberculosis or other systemic infectious process by review of systems and physical examination
4. Have a history of immunodeficiency, autoimmune disease, or use of immunosuppressive medications
5. Current treatment for malignancy other than basal or squamous cell carcinoma of the skin or carcinoma in situ of the cervix
6. Is pregnant
7. History of any chronic illness that would interfere with conduct or completion of study(as determined by the investigator)
8. Have evidence of psychiatric, medical and/or substance abuse problems during the past 24 weeks that the investigator believes would adversely affect the volunteer's ability to participate in the trial
9. Have occupational or other responsibilities that would prevent completion of participation in the study
10. Have received any live, attenuated vaccine except rabies vaccine within 60 days of study entry
11. Vaccine (FDA approved; e.g. influenza, pneumovax, etc) administration within 30 days of immunization with the study vaccine. NOTE: Medically indicated subunit or killed vaccines (e.g., Hepatitis A or Hepatitis B) should be given prior to trial initiation or after completion of the study immunizations. If patient requires immunization, injections should be given more than 2 weeks prior or 2 weeks after study immunization
12. Have used experimental therapeutic agents within 30 days of study entry
13. Have received blood products or immunoglobulins in the past 12 weeks
14. Have a history of anaphylaxis or other serious adverse reactions to vaccines
15. Have previously received an HIV vaccine
16. Volunteers with any of the following laboratory parameters at the screening visit (within 30 days of immunization): Hemoglobin \<10 (without having received a blood or red blood cell transfusion within 30 days prior to laboratory test); neutrophil count \<750 cells/mm3; platelet count \<50,000/mm3; serum creatinine \> 2.0 mg/dL; aspartate aminotransferase (AST) or alanine aminotransferase (ALT) \> 3 times the upper limits of normal; total bilirubin \> 1.5 mg/dL
17. Pregnant women or women who are breast-feeding; female volunteers of childbearing potential who are not using or willing to use an effective (as judged by the investigator) barrier contraceptive methods or abstinence while enrolled in this study.
18. Use of any immune modulators or suppressors within 45 days of study entry including but not limited to agents such as interleukins (e.g. IL-2), interferons (e.g. IFN-\*), high dose systemic steroids (e.g. ≥ 20 mg prednisone equivalent/day) for \> 30 days, thalidomide, filgrastim (G-CSF), sargramostim (GM-CSF), dinitrochlorobenzene (DNCB), thymosin alpha, thymopentin, inosiplex, polyribonucleoside, ditiocarb sodium, cyclosporin, mycophenolate mofetil, methotrexate, and cancer chemotherapy.
19. No other investigational agent within 30 days of study entry
20. Any other condition which, in the opinion of the investigator, might interfere with completion of the study or evaluation of the results
21. Have active Hepatitis B virus infection (positive HBsAg) or Hepatitis C infection(defined as positive antibodies)
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Bill and Melinda Gates Foundation

OTHER

Sponsor Role collaborator

Auro Vaccines LLC

INDUSTRY

Sponsor Role collaborator

University of Maryland, Baltimore

OTHER

Sponsor Role lead

Responsible Party

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Mohammad Sajadi

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Charles E Davis, M.D.

Role: PRINCIPAL_INVESTIGATOR

Institute of Human Virology

Locations

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University of Maryland, Institute of Human Virology

Baltimore, Maryland, United States

Site Status

Countries

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United States

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form

View Document

Other Identifiers

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HP-00065547

Identifier Type: -

Identifier Source: org_study_id