Trial Outcomes & Findings for Intranasal Oxytocin and Maternal Neglect (NCT NCT02737436)

NCT ID: NCT02737436

Last Updated: 2025-01-28

Results Overview

We will compare the BOLD signal of regions in the brain (including the ventral striatum, caudate, putamen, amygdala, and the medial prefrontal cortex) in mothers who receive intranasal oxytocin, compared to placebo. In every analysis, we measure the difference in brain response (BOLD signal) between viewing the mother's own infant (O) more than unknown infant (U) faces (O\>U), during fMRI scanning.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

132 participants

Primary outcome timeframe

30 minutes after intranasal spray is administered

Results posted on

2025-01-28

Participant Flow

132 mothers signed a consent form and enrolled in the study. Of these, 74 had at least one scanning session (and were assigned to an Arm), and 58 completed both scanning sessions. 45 individuals were included in the final scanning analysis, each of whom had 2 completed scans and usable data.

Unit of analysis: Scan

Participant milestones

Participant milestones
Measure
PLACEBO FIRST GROUP: Functional MRI (fMRI) Following Placebo, Then fMRI Following Oxytocin
Intranasal spray given 50 minutes prior to fMRI scanning. On the first scanning visit, the participant received placebo; on the second scanning visit the participant received oxytocin.
OXYTOCIN FIRST GROUP: fMRI Following Oxytocin, Then fMRI Following Placebo
Intranasal spray given 50 minutes prior to fMRI scanning. On the first scanning visit, the participant received oxytocin (24 IU); on the second scanning visit the participant received placebo.
First Intervention (Day of 1st Scan)
STARTED
30 30
44 44
First Intervention (Day of 1st Scan)
COMPLETED
30 30
44 44
First Intervention (Day of 1st Scan)
NOT COMPLETED
0 0
0 0
Washout Period
STARTED
30 30
44 44
Washout Period
COMPLETED
25 25
33 33
Washout Period
NOT COMPLETED
5 5
11 11
Second Intervention (Day of 2nd Scan)
STARTED
25 25
33 33
Second Intervention (Day of 2nd Scan)
COMPLETED
25 25
33 33
Second Intervention (Day of 2nd Scan)
NOT COMPLETED
0 0
0 0
Data Analysis
STARTED
25 50
33 66
Data Analysis
COMPLETED
20 40
25 50
Data Analysis
NOT COMPLETED
5 10
8 16

Reasons for withdrawal

Reasons for withdrawal
Measure
PLACEBO FIRST GROUP: Functional MRI (fMRI) Following Placebo, Then fMRI Following Oxytocin
Intranasal spray given 50 minutes prior to fMRI scanning. On the first scanning visit, the participant received placebo; on the second scanning visit the participant received oxytocin.
OXYTOCIN FIRST GROUP: fMRI Following Oxytocin, Then fMRI Following Placebo
Intranasal spray given 50 minutes prior to fMRI scanning. On the first scanning visit, the participant received oxytocin (24 IU); on the second scanning visit the participant received placebo.
Washout Period
Lost to Follow-up
5
11
Data Analysis
Scanning data problems
5
8

Baseline Characteristics

Intranasal Oxytocin and Maternal Neglect

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Study Participants
n=45 Participants
Functional MRI scanning: All study participants underwent two functional MRI scanning visits. On one occasion this was after receiving an intranasal dose of oxytocin; on the other occasions, after receiving intranasal placebo dose.
Age, Categorical
<=18 years
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
45 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
Age, Continuous
30.6 years
STANDARD_DEVIATION 4.4 • n=5 Participants
Sex: Female, Male
Female
45 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
7 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
35 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
3 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
4 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
6 Participants
n=5 Participants
Race (NIH/OMB)
White
28 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
7 Participants
n=5 Participants

PRIMARY outcome

Timeframe: 30 minutes after intranasal spray is administered

Population: Participants were included if they completed both scans AND had good quality data on both scans. As pre-specified in the study protocol and similar to most functional MRI studies, the contrasts between two stimuli are reported (e.g., own vs unknown baby faces).

We will compare the BOLD signal of regions in the brain (including the ventral striatum, caudate, putamen, amygdala, and the medial prefrontal cortex) in mothers who receive intranasal oxytocin, compared to placebo. In every analysis, we measure the difference in brain response (BOLD signal) between viewing the mother's own infant (O) more than unknown infant (U) faces (O\>U), during fMRI scanning.

Outcome measures

Outcome measures
Measure
Placebo
n=45 Participants
Intranasal placebo spray given 50 minutes prior to scanning Placebo: Intranasal placebo spray given 50 minutes prior to scanning Functional MRI scanning: All study participants underwent two functional MRI scanning visits. On one occasion this was after receiving an intranasal dose of oxytocin; on the other occasions, after receiving intranasal placebo dose.
Oxytocin
n=45 Participants
Intranasal oxytocin (24IU) given 50 minutes prior to scanning Intranasal Oxytocin: Intranasal oxytocin (24IU) given 50 minutes prior to scanning Functional MRI scanning: All study participants underwent two functional MRI scanning visits. On one occasion this was after receiving an intranasal dose of oxytocin; on the other occasions, after receiving intranasal placebo dose.
Differences in Brain Activation (Blood Oxygen Level Dependent [BOLD] Signal) of Dopamine Reward Regions and Prefrontal Cortex After Intranasal Oxytocin Administration in Mothers
Region of Interest (ROI) analysis (O>U): Ventral striatum activation
11.28 BOLD signal
Interval -1.71 to 24.27
11.06 BOLD signal
Interval -3.64 to 25.76
Differences in Brain Activation (Blood Oxygen Level Dependent [BOLD] Signal) of Dopamine Reward Regions and Prefrontal Cortex After Intranasal Oxytocin Administration in Mothers
ROI analysis (O>U): Caudate activation
9.01 BOLD signal
Interval -2.54 to 20.56
43.32 BOLD signal
Interval 28.29 to 58.35
Differences in Brain Activation (Blood Oxygen Level Dependent [BOLD] Signal) of Dopamine Reward Regions and Prefrontal Cortex After Intranasal Oxytocin Administration in Mothers
ROI analysis (O>U): Putamen activation
20.60 BOLD signal
Interval 11.72 to 29.48
25.34 BOLD signal
Interval 13.27 to 37.41
Differences in Brain Activation (Blood Oxygen Level Dependent [BOLD] Signal) of Dopamine Reward Regions and Prefrontal Cortex After Intranasal Oxytocin Administration in Mothers
ROI analysis (O>U): Amygdala activation
25.57 BOLD signal
Interval 15.77 to 35.37
29.14 BOLD signal
Interval 14.17 to 44.11
Differences in Brain Activation (Blood Oxygen Level Dependent [BOLD] Signal) of Dopamine Reward Regions and Prefrontal Cortex After Intranasal Oxytocin Administration in Mothers
ROI analysis (O>U): Medial prefrontal cortex activation
-9.93 BOLD signal
Interval -19.98 to 0.12
9.58 BOLD signal
Interval 0.45 to 18.71

Adverse Events

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Oxytocin

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr Lane Strathearn

University of Iowa

Phone: 2193849464

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place